Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,496,512 C→T 10.0% intergenic (+23/‑163) leuX → / → intB tRNA‑Leu/pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,496,5120CT10.0% 376.6 / 20.3 130intergenic (+23/‑163)leuX/intBtRNA‑Leu/pseudogene, integrase homology;IS, phage, Tn; Phage‑related functions and prophages; KpLE2 phage‑like element; P4‑like integrase
Reads supporting (aligned to +/- strand):  ref base C (52/65);  new base T (5/8);  total (57/73)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 133 total aligned reads displayed.

CGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACA  >  NC_000913/4496363‑4496661
                                                                                                                                                     |                                                                                                                                                     
cGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGTAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAAc                                                                                                                                                       >  1:972605/1‑150 (MQ=255)
  aCAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTg                                                                                                                                                     <  1:1031544/150‑1 (MQ=255)
  aCAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTg                                                                                                                                                     >  2:1648279/1‑150 (MQ=255)
     ggTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAgg                                                                                                                                                  >  2:628549/1‑150 (MQ=255)
       tataATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTc                                                                                                                                                <  1:1142275/150‑1 (MQ=255)
           aTCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtatt                                                                                                                                            <  1:1052994/150‑1 (MQ=255)
           aTCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttt                                                                                                                                            >  1:952/1‑150 (MQ=255)
           aTCCACAACGTTTTCCGACTACCTCTTCAGTGCCGAAGTGGCGAAATCTGTAGAAGCAGTTGATTCAAAATCAAACGTAGAAATACGTTCCGGTTCGAGTCCTGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtatt                                                                                                                                            <  1:1072770/150‑1 (MQ=255)
            tCCACAACGTGTTGCGCATACCTCTTCAGTGCCGTAGTGGCGAAATCGGTTGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTGCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtattt                                                                                                                                           <  2:1097382/150‑1 (MQ=255)
              cacaACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtatttac                                                                                                                                         <  1:1542705/150‑3 (MQ=255)
               acaACGTTTTCCGCATCCCTCTTCAGTGCCGAAGGGGCGAAATCGGTAGACGCATTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGGCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTa                                                                                                                                        <  2:1076234/150‑1 (MQ=255)
                   cGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGcc                                                                                                                                    >  2:612165/1‑150 (MQ=255)
                     ttttCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAGCCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTg                                                                                                                                  <  1:1666706/150‑1 (MQ=255)
                     ttttCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTg                                                                                                                                  <  1:984312/150‑1 (MQ=255)
                         ccGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCAGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAAt                                                                                                                              >  2:71179/1‑150 (MQ=255)
                           gCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGATGTCTTTTTTTATGCCTGAAATcc                                                                                                                            <  2:1042209/150‑1 (MQ=255)
                           gCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATcc                                                                                                                            <  2:882316/150‑1 (MQ=255)
                             aTACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCTTAGAAATACGTGCCGGTTCGAGTGCGGCCTTCGGCACCAAAACTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAg                                                                                                                          <  1:706948/150‑1 (MQ=255)
                             aTACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAg                                                                                                                          <  2:1001870/150‑1 (MQ=255)
                              tACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgt                                                                                                                         >  1:34444/1‑150 (MQ=255)
                                 ctctTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCATAATCAACCGTAGAAATCCGTGCCGGTTCGATTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt                                                                                                                      <  1:538063/150‑1 (MQ=255)
                                     tCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatc                                                                                                                  <  2:1476244/150‑1 (MQ=255)
                                        gTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatatat                                                                                                               >  1:1080841/1‑150 (MQ=255)
                                         tGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatctatc                                                                                                              <  2:377545/150‑1 (MQ=255)
                                         tGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatctatc                                                                                                              <  2:929976/150‑1 (MQ=255)
                                           ccGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACAAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCtt                                                                                                            <  2:572544/150‑1 (MQ=255)
                                              ttttGGCGTAATCGTTTGACTCATTTGATTCAAATTCAACCTTTGAAATACGTGGCTGTTCGTGTCCGGCATTCGGCACCATAAGTATGTATATAGACCTCTTCTGAGGTCTTTTTTTATGCCTGTAATCCAGTGTTTATCTATCATTcc                                                                                                         <  2:335346/147‑1 (MQ=255)
                                               aGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTccc                                                                                                        <  2:952/150‑1 (MQ=255)
                                               aGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTccc                                                                                                        <  2:1256619/150‑1 (MQ=255)
                                                 tGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGc                                                                                                      >  1:312860/1‑150 (MQ=255)
                                                 tGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGc                                                                                                      >  2:299086/1‑150 (MQ=255)
                                                  ggCGAAATCGGTAGACGCAGTTGATTCAAAATCACCCGTAGAAATACGTGCCGGTTCGCGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGGCTTTTTTTATGCCGGAAATCCAGGGTTTATCTATCGTTCCCggt                                                                                                     >  1:320590/1‑148 (MQ=255)
                                                  ggCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCt                                                                                                     >  1:1613866/1‑150 (MQ=255)
                                                         tCGGTAGACGCAGTTGATTCATAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTaa                                                                                              >  2:1254441/1‑150 (MQ=255)
                                                          cgttAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCTGCCTTCGGCACCAAACGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAAc                                                                                             <  1:890059/147‑1 (MQ=255)
                                                           ggTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAAct                                                                                            <  2:1485131/150‑1 (MQ=255)
                                                               gACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAActctct                                                                                        >  2:546762/1‑150 (MQ=255)
                                                               gACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAActctct                                                                                        <  1:1494094/150‑1 (MQ=255)
                                                               gACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAActctct                                                                                        >  2:395570/1‑150 (MQ=255)
                                                                  gCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCaa                                                                                     >  1:709507/1‑150 (MQ=255)
                                                                     gTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGt                                                                                  <  2:1151765/150‑1 (MQ=255)
                                                                     gTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGt                                                                                  >  1:1203402/1‑150 (MQ=255)
                                                                      ttGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTc                                                                                 <  1:787446/150‑1 (MQ=255)
                                                                       tGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCa                                                                                <  2:1399383/150‑1 (MQ=255)
                                                                          ttCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAAcc                                                                             <  2:244191/150‑1 (MQ=255)
                                                                          ttCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAAcc                                                                             >  2:378521/1‑150 (MQ=255)
                                                                           tCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCg                                                                            >  2:771163/1‑150 (MQ=255)
                                                                            cAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCATGAAATCCAGTGTTTATCTATCTTTCCAGCTATATTAACTCTATCAAGGTCAACCGa                                                                           >  2:1014396/1‑150 (MQ=255)
                                                                             aaaaTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGat                                                                          >  1:128448/1‑150 (MQ=255)
                                                                              aaaTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGata                                                                         <  1:628549/150‑1 (MQ=255)
                                                                                aTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATc                                                                       <  2:1299278/150‑1 (MQ=255)
                                                                                         aGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACAt                                                              >  1:662339/1‑150 (MQ=255)
                                                                                         aGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACATCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTAcct                                                              >  2:1310326/1‑148 (MQ=255)
                                                                                          gAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATc                                                             >  2:325254/1‑150 (MQ=255)
                                                                                           aaaTACGTGCCGGTTCGAGTCCTGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCt                                                            <  1:1172398/150‑1 (MQ=255)
                                                                                           aaaTACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCt                                                            <  2:1332551/150‑1 (MQ=255)
                                                                                             aTACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCCACTGAGGTCTTTTTTTATGGCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGAACCTCTAc                                                          >  1:117932/1‑150 (MQ=255)
                                                                                                  tGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGATGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACa                                                     <  1:1211689/150‑1 (MQ=255)
                                                                                                  tGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACa                                                     >  1:1370721/1‑150 (MQ=255)
                                                                                                      ggTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATg                                                 >  1:940792/1‑150 (MQ=255)
                                                                                                      ggTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATg                                                 <  2:803723/150‑1 (MQ=255)
                                                                                                       gTTCGAGTCAGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGt                                                >  2:1163433/1‑150 (MQ=255)
                                                                                                           gAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGt                                            <  1:612165/150‑1 (MQ=255)
                                                                                                             gTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTAc                                          >  1:1361162/1‑150 (MQ=255)
                                                                                                               ccGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAg                                        >  1:1434996/1‑150 (MQ=255)
                                                                                                                cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAga                                       >  1:1556099/1‑150 (MQ=255)
                                                                                                                cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAga                                       >  1:28269/1‑150 (MQ=255)
                                                                                                                 ggacTTCGGCACGAAAAGTATGTAAATAGACATCAACTGTGGTCTTTTTTTATGCCTGAAATCCAGTGTTAATCTATCTATCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgat                                      <  1:1036161/147‑1 (MQ=255)
                                                                                                                  gCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatg                                     >  2:474408/1‑150 (MQ=255)
                                                                                                                   ccTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATAATAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAgatga                                    <  2:17321/150‑1 (MQ=255)
                                                                                                                      tCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATgg                                 <  2:1370721/150‑1 (MQ=255)
                                                                                                                      tCGGAACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATgg                                 <  2:701642/150‑1 (MQ=255)
                                                                                                                       cGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGt                                <  1:156247/150‑1 (MQ=255)
                                                                                                                        ggCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCTCTATATTATCTCTCTCAAGGTCATCCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTa                               <  1:99727/150‑1 (MQ=255)
                                                                                                                          cACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTAtt                             <  2:865485/150‑1 (MQ=255)
                                                                                                                             cAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAAAATATGTTGGTACTGATGATGGTAtttgc                          >  1:240068/1‑148 (MQ=255)
                                                                                                                              aaaaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTTTCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCg                         <  1:560319/150‑1 (MQ=255)
                                                                                                                              aaaaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCg                         <  1:1169711/150‑1 (MQ=255)
                                                                                                                              aaaaGTATGTAAATAGACCTCAACTGAGGTATTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCACTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCg                         >  2:956988/1‑150 (MQ=255)
                                                                                                                                aaGTATGTAAATAGAACTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACACATGTTGGTACAGATGATGGTATTTCCGGt                       <  2:775203/150‑1 (MQ=255)
                                                                                                                                 ggcataaaaaaAGACCTGAGTTGAGGTCTATTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTATCTCTCTAAAGGTCAACCGATATAAACGTACATCTACCACCATATGTTGGTAAAGATGATGGTATTTCAGGtt                      >  1:102504/8‑150 (MQ=255)
                                                                                                                                  gTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTc                     <  1:305338/150‑1 (MQ=255)
                                                                                                                                      aaaaaaAGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATa                 >  2:45471/3‑150 (MQ=255)
                                                                                                                                        aaaaAGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAAt               >  2:1340929/1‑150 (MQ=255)
                                                                                                                                        aaaaAGACCTCAGTTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAAt               >  1:457959/1‑150 (MQ=255)
                                                                                                                                        aaaaAGACCTCAGTTGAGGTCTTTTTTTAAGCCTGAAATCCAGTGTTTATCTATATTTCCCGCTATATTAACTCTCTCAAGCTCAACCGATATCAACGTACATCTACCAACATATGTTTGTACAGATCATGGTATTTCCGGTACGATAAt               >  2:1636593/1‑150 (MQ=255)
                                                                                                                                         aaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATg              <  2:34444/150‑1 (MQ=255)
                                                                                                                                         aaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCAGGTGCGATAATg              >  1:1490270/1‑150 (MQ=255)
                                                                                                                                           tAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCt            <  2:709507/150‑1 (MQ=255)
                                                                                                                                            aGACCTCAACTGAGGTCTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTg          <  2:748433/150‑1 (MQ=255)
                                                                                                                                             taCCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTg          <  1:1648279/149‑1 (MQ=255)
                                                                                                                                             gACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTg          >  1:804313/1‑150 (MQ=255)
                                                                                                                                             gACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTg          >  2:1329679/1‑150 (MQ=255)
                                                                                                                                                 tCAACTGATGTCTTTTTTTATTCCTGAAATCCAGTTTTTATCTATCTTTCCAGCTATATTAACTCTCTCAAGGTCAACAGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTAcc      <  1:329661/150‑1 (MQ=255)
                                                                                                                                                 tCAACTGATGTATTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTTTTTCCGGTTCGATAATGCTTGTAcc      <  1:876911/150‑1 (MQ=255)
                                                                                                                                                 tCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTAcc      <  1:1332796/150‑1 (MQ=255)
                                                                                                                                                 tCAACTGAGGTCTTTTTGTCAGCCTGAAATCCAGGGTTTATCTAGCTTTCCCGCTATATTACCTCTCTCCAGGTCCCCCGATAGCACCGGACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTAcc      <  1:334653/150‑1 (MQ=255)
                                                                                                                                                 gcAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAGCCGATATCATCGTACATCTACCAACATATGTTGGTACAGATGATCGTATTTCCGGTTCGATAATACTTGTagc      >  1:221202/2‑148 (MQ=255)
                                                                                                                                                    aCTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAAc   <  2:1485215/150‑1 (MQ=255)
                                                                                                                                                    aCTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAAc   >  2:21964/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAGGTCAACCGATATCAACGTACATCTACCAACATATGTTGGTACAGATGATGGTATTTCCGGTTCGATAATGCTTGTACCAACA  >  NC_000913/4496363‑4496661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: