BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,499,887	G→C	5.0%	pseudogene (648/942 nt)	yjgX ←	pseudogene fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,499,887	0	G	C	5.0%	400.2 / 9.1	119	pseudogene (648/942 nt)	yjgX	pseudogene fragment
Reads supporting (aligned to +/- strand): ref base G (55/58); new base C (2/4); total (57/62)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.78e-01

Only 100 of 121 total aligned reads displayed.

CCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATT  >  NC_000913/4499740‑4500036

 cACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGAcc                                                                                                                                                    <  2:1236765/150‑1 (MQ=255)

 cACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGAcc                                                                                                                                                    <  1:1720059/150‑1 (MQ=255)

  aCTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCg                                                                                                                                                   >  1:540167/1‑150 (MQ=255)

  aCTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTAGCTGACCg                                                                                                                                                   >  1:108617/1‑150 (MQ=255)

     gAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACa                                                                                                                                                >  1:1575970/1‑150 (MQ=255)

     gAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACa                                                                                                                                                >  2:1596/1‑150 (MQ=255)

        aaaTACCTGGGAAATGTCTGGCACTGATTTTTCTACTGACGGGATGATGAGACGTTCTGTCACTGGCGGTAATAATCAGTGGGACACTGTAGCCTCCCTGGAAATGACCATAATGAGGAACAGGATTGGCACTGTCGCTGACAGACAGcc                                                                                                                                             <  2:1154489/150‑1 (MQ=255)

         aaTACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGGTCAGCGACAgtgc                                                                                                                                            <  2:1498201/150‑4 (MQ=255)

         aaTACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGG‑GGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCa                                                                                                                                           >  2:1258101/1‑150 (MQ=255)

          aTACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGGTCAGCGACAgtgcc                                                                                                                                           <  2:1576202/150‑5 (MQ=255)

           tACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGGTCAGCGACAgtgcca                                                                                                                                          <  1:1322508/150‑6 (MQ=255)

           tACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCaa                                                                                                                                          >  1:1008575/1‑150 (MQ=255)

             ccTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAgg                                                                                                                                        <  2:1262337/150‑1 (MQ=255)

             ccTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAgg                                                                                                                                        >  1:1370269/1‑150 (MQ=255)

             ccTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAgg                                                                                                                                        <  2:511266/150‑1 (MQ=255)

             ccTGCGAAATGACGGGCAATTATTTTTCTACAGAACGGCTGATGATACGTTAATTCAATGGCGATAATAATCAGTGGCACACTGTAGAATCCATGCACATGACCCTAATGATGAACAGGATTGGCCCTGTCGCTGACCGCCCGCCCACgg                                                                                                                                        >  2:75130/1‑150 (MQ=255)

               tGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGcc                                                                                                                                      <  1:1702404/150‑1 (MQ=255)

                gCGAAATGACGGGCACTGATTTTTCTACTGACTGGCTGATGAGACGTTATGTCACTGGCGGTAATAATAAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCa                                                                                                                                     <  2:1298350/150‑1 (MQ=255)

                gCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCa                                                                                                                                     >  1:1016206/1‑150 (MQ=255)

                 cGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAg                                                                                                                                    >  2:792349/1‑150 (MQ=255)

                 cGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCAAAATGATGAACAGGATTGGCACTGTCGATGACCGACAGCCCAAGGCCAg                                                                                                                                    >  2:189856/1‑150 (MQ=255)

                      tGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAg                                                                                                                               >  2:1688666/1‑150 (MQ=255)

                      tGAAGGGCACTGATTATTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACACGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAg                                                                                                                               <  1:23624/150‑1 (MQ=255)

                        actggCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTa                                                                                                                             <  1:411935/147‑1 (MQ=255)

                        aCGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTa                                                                                                                             >  1:1261647/1‑150 (MQ=255)

                         cGGGCACTGATTATTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTaa                                                                                                                            <  2:318169/150‑1 (MQ=255)

                            gCACTGATTAATCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGAACATAAAGATGAACAGGATAGGCACTGACGCTGACCGACAGCCAAAGGCAAGAAAAGTAAAGc                                                                                                                         >  1:1511916/1‑150 (MQ=255)

                             cACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCa                                                                                                                        >  2:642086/1‑150 (MQ=255)

                                tGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATgg                                                                                                                     <  1:875881/150‑1 (MQ=255)

                                tGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATgg                                                                                                                     <  1:1278954/150‑1 (MQ=255)

                                 gATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAACGCATGGc                                                                                                                    >  1:137480/1‑150 (MQ=255)

                                 gATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGc                                                                                                                    >  2:1329315/1‑150 (MQ=255)

                                 gATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGc                                                                                                                    >  1:1569341/1‑150 (MQ=255)

                                 gATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGc                                                                                                                    >  2:733431/1‑150 (MQ=255)

                                 gATTTTTCTACTGACGGGATGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGAACGACAGCCCAATGCAAGAAAAGTAAAGCATGGc                                                                                                                    >  1:244435/1‑150 (MQ=255)

                                 gATTTTTCTACTGACCGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGc                                                                                                                    <  2:453865/150‑1 (MQ=255)

                                    ttttCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGaa                                                                                                                 >  1:946227/1‑150 (MQ=255)

                                       tCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATg                                                                                                              >  2:1298805/1‑150 (MQ=255)

                                        cTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGa                                                                                                             <  1:1512348/150‑1 (MQ=255)

                                            tGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTg                                                                                                         <  1:553968/150‑1 (MQ=255)

                                              aCGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTTGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACACGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAg                                                                                                       <  1:1543641/150‑1 (MQ=255)

                                                gggCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTa                                                                                                     >  2:676559/1‑150 (MQ=255)

                                                gggCTGATGAGACGTTATCTCACTGGCGGTAATAATCAGTGGCACACTGTAGACTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTa                                                                                                     <  1:75130/150‑1 (MQ=255)

                                                  gCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAG‑CCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCg                                                                                                  <  2:533413/150‑1 (MQ=255)

                                                   cTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCg                                                                                                  >  2:392564/1‑150 (MQ=255)

                                                     gatgaGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGc                                                                                                >  1:1014286/1‑150 (MQ=255)

                                                            cGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGAt                                                                                         <  2:1575970/150‑1 (MQ=255)

                                                            cGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGAt                                                                                         >  2:1139240/1‑150 (MQ=255)

                                                              ttATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATAc                                                                                       <  2:1008575/150‑1 (MQ=255)

                                                              ttATGTCACTGG‑GGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATAcc                                                                                      <  1:1258101/150‑1 (MQ=255)

                                                                 tGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCAt                                                                                    <  1:313528/150‑1 (MQ=255)

                                                                 tGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCAt                                                                                    >  2:527220/1‑150 (MQ=255)

                                                                   tCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCa                                                                                  <  1:1660100/150‑1 (MQ=255)

                                                                   tCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCa                                                                                  <  1:792349/150‑1 (MQ=255)

                                                                         gCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTgg                                                                            <  2:1005170/150‑1 (MQ=255)

                                                                              aataatCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAg                                                                       <  2:640132/150‑1 (MQ=255)

                                                                               ataatCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGa                                                                      >  1:1665263/1‑150 (MQ=255)

                                                                                taatCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGaa                                                                     >  1:719508/1‑150 (MQ=255)

                                                                                   tCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCGAGCTGGCCAAGAAAGt                                                                  <  1:1262747/150‑1 (MQ=255)

                                                                                     aGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTa                                                                <  2:626164/150‑1 (MQ=255)

                                                                                       tGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGACGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTGATc                                                              >  2:1120314/1‑150 (MQ=255)

                                                                                        ggCACACTGTAGCCTCCCTGCACATGACCATAATGATGTACAGGATTGGCAGTGTCGCTGACCGAGTGCCCAAGGCCTGAAAAGTATAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATcc                                                             <  2:1511916/150‑1 (MQ=255)

                                                                                        ggCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATcc                                                             <  2:222900/150‑1 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTTGCACTGTCGCTGACCGAAAGCCCAAGGCCAGAAAATTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATcaa                                                            >  2:1552256/1‑148 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGt                                                                  >  2:50031/1‑144 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCa                                                            <  2:719508/150‑1 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCAGCACATGACCATAATGATGAACAGGTTTGGCACTGTCGCTGACCGACAGCCAAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGAGACCATCAAGCTGGCCAAGAAAGTTATCCa                                                            <  1:290892/150‑1 (MQ=255)

                                                                                            cacTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGtt                                                         <  2:622638/150‑1 (MQ=255)

                                                                                              cTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTa                                                       >  1:981548/1‑150 (MQ=255)

                                                                                                gTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACt                                                     <  1:1139240/150‑1 (MQ=255)

                                                                                                  aGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTATAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCCGTGTACTGa                                                   >  2:1245284/1‑150 (MQ=255)

                                                                                                  aGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGa                                                   <  2:946227/150‑1 (MQ=255)

                                                                                                   gCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGATAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGTAGGATACCATCAAGCTGGCCTAGAAAGTTATCCAGTTTACTGAt                                                  <  2:747180/150‑1 (MQ=255)

                                                                                                   gCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGAt                                                  <  1:1355981/150‑1 (MQ=255)

                                                                                                   gCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGAt                                                  >  2:640110/1‑150 (MQ=255)

                                                                                                       cccTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTg                                              >  2:1284272/1‑150 (MQ=255)

                                                                                                       cccTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGATGACCGACAGCCCAACGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAACGTTATCCAGTTTACTGATGCTg                                              >  2:120548/1‑150 (MQ=255)

                                                                                                         cTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGc                                            >  2:91277/1‑150 (MQ=255)

                                                                                                           tcacaTTACCATAATGATGAACAGGTTTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAATAGTAAAGCATGGCGAAATGACGTGAGTACCGGAGAAGGATACCATCAAGCTGGCCAAGAATGTTATCCAGTTTACTGATGCTGGCGa                                          <  1:189856/149‑1 (MQ=255)

                                                                                                            cacaTGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAg                                         <  2:1370269/150‑1 (MQ=255)

                                                                                                             acaTGACCATAATGATGATCAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAATTTATCCAGTTTACTGATGCTGGCGAgg                                        >  2:230530/1‑150 (MQ=255)

                                                                                                             acaTGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAgg                                        >  1:782983/1‑150 (MQ=255)

                                                                                                               aTGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTa                                      <  1:1688666/150‑1 (MQ=255)

                                                                                                                 gACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTAATCAAGATTACTGATGCTGGCGAGGTaaa                                    >  2:1382711/1‑149 (MQ=255)

                                                                                                                         tgatgaACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAAtt                            <  2:1642115/150‑1 (MQ=255)

                                                                                                                         tgatgaACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGGTTACTGCTGCGGGCGAGGTACCAGGCAAtt                            >  1:209401/1‑150 (MQ=255)

                                                                                                                              aaCAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCg                       >  1:163227/1‑150 (MQ=255)

                                                                                                                               aCAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGACAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCgg                      <  2:1059790/150‑1 (MQ=255)

                                                                                                                               aCAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCgg                      <  1:1709396/150‑1 (MQ=255)

                                                                                                                                   gATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGAt                  >  2:421639/1‑150 (MQ=255)

                                                                                                                                    ccttgggcTGTCGGTCAGCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATa                 >  2:1633086/8‑150 (MQ=255)

                                                                                                                                     ttGGCACTGTCGCTGCCCGACAGCCCACGGCCAGAAAAGTAACGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCAGCACGGGGGCCTAGAAATTTATCCAGTTGACGGATGGTGGCGCGGTACCCGGCATTTTGGCggggggcg                >  2:848094/1‑146 (MQ=255)

                                                                                                                                     ttGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATAc                >  1:1352714/1‑150 (MQ=255)

                                                                                                                                     cttgggcTGTCGGTCAGCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATAc                >  1:577181/7‑150 (MQ=255)

                                                                                                                                       ggCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTg              <  2:1014286/150‑1 (MQ=255)

                                                                                                                                       ggCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTg              >  2:1694457/1‑150 (MQ=255)

                                                                                                                                        gCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGc             <  1:733431/150‑1 (MQ=255)

                                                                                                                                        gCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGc             <  2:1155635/150‑1 (MQ=255)

                                                                                                                                              tCGCTGACCGACATCCCAAGGCCAGAAAAGTAAAGCATGGCGAACTGACGTGAGTAAGGGCGAAGGATACCATCAAGCTGGCCAAGAAATTTATCCAGTTTACTGATGCTGGCCAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAgg       <  2:244435/150‑1 (MQ=255)

                                                                                                                                                   gACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAatt  >  1:142902/1‑150 (MQ=255)

CCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATT  >  NC_000913/4499740‑4500036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`