BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,499,889	C→G	5.0%	pseudogene (646/942 nt)	yjgX ←	pseudogene fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,499,889	0	C	G	5.0%	387.7 / 9.3	119	pseudogene (646/942 nt)	yjgX	pseudogene fragment
Reads supporting (aligned to +/- strand): ref base C (56/56); new base G (2/4); total (59/60)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

Only 100 of 125 total aligned reads displayed.

CCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTAT  >  NC_000913/4499740‑4500038

ccACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTAACTGGCTGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGAc                                                                                                                                                       <  1:1143264/150‑1 (MQ=255)

 cACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGAcc                                                                                                                                                      <  2:1236765/150‑1 (MQ=255)

 cACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGAcc                                                                                                                                                      <  1:1720059/150‑1 (MQ=255)

 cACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGAcc                                                                                                                                                      >  2:439009/1‑150 (MQ=255)

  aCTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCg                                                                                                                                                     >  1:540167/1‑150 (MQ=255)

  aCTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTAGCTGACCg                                                                                                                                                     >  1:108617/1‑150 (MQ=255)

   cTGAAAAATACCAGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGCCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGa                                                                                                                                                    >  1:747180/1‑150 (MQ=255)

     gAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACa                                                                                                                                                  >  2:1596/1‑150 (MQ=255)

     gAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACa                                                                                                                                                  >  1:1575970/1‑150 (MQ=255)

        aaaTACCTGGGAAATGTCTGGCACTGATTTTTCTACTGACGGGATGATGAGACGTTCTGTCACTGGCGGTAATAATCAGTGGGACACTGTAGCCTCCCTGGAAATGACCATAATGAGGAACAGGATTGGCACTGTCGCTGACAGACAGcc                                                                                                                                               <  2:1154489/150‑1 (MQ=255)

         aaTACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGGTCAGCGACAgtgc                                                                                                                                              <  2:1498201/150‑4 (MQ=255)

          aTACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGGTCAGCGACAgtgcc                                                                                                                                             <  2:1576202/150‑5 (MQ=255)

           tACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGGTCAGCGACAgtgcca                                                                                                                                            <  1:1322508/150‑6 (MQ=255)

           tACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGGTCAGCGACAgtgcca                                                                                                                                            <  1:898602/150‑6 (MQ=255)

           tACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCaa                                                                                                                                            >  1:1008575/1‑150 (MQ=255)

             ccTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAgg                                                                                                                                          <  2:1262337/150‑1 (MQ=255)

             ccTGCGAAATGACGGGCAATTATTTTTCTACAGAACGGCTGATGATACGTTAATTCAATGGCGATAATAATCAGTGGCACACTGTAGAATCCATGCACATGACCCTAATGATGAACAGGATTGGCCCTGTCGCTGACCGCCCGCCCACgg                                                                                                                                          >  2:75130/1‑150 (MQ=255)

                gCGAAATGACGGGCACTGATTTTTCTACTGACTGGCTGATGAGACGTTATGTCACTGGCGGTAATAATAAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCa                                                                                                                                       <  2:1298350/150‑1 (MQ=255)

                gCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCa                                                                                                                                       >  1:1016206/1‑150 (MQ=255)

                 cGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAg                                                                                                                                      >  2:792349/1‑150 (MQ=255)

                 cGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCAAAATGATGAACAGGATTGGCACTGTCGATGACCGACAGCCCAAGGCCAg                                                                                                                                      >  2:189856/1‑150 (MQ=255)

                      tGACGGGCAGTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAg                                                                                                                                 <  2:656207/150‑1 (MQ=255)

                      tGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAg                                                                                                                                 >  2:1688666/1‑150 (MQ=255)

                      tGAAGGGCACTGATTATTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACACGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAg                                                                                                                                 <  1:23624/150‑1 (MQ=255)

                        aCGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTa                                                                                                                               >  1:1261647/1‑150 (MQ=255)

                         cGGGCACTGATTATTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTaa                                                                                                                              <  2:318169/150‑1 (MQ=255)

                            gCACTGATTAATCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGAACATAAAGATGAACAGGATAGGCACTGACGCTGACCGACAGCCAAAGGCAAGAAAAGTAAAGc                                                                                                                           >  1:1511916/1‑150 (MQ=255)

                             cACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCa                                                                                                                          >  2:642086/1‑150 (MQ=255)

                                tGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATgg                                                                                                                       <  1:875881/150‑1 (MQ=255)

                                 gATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAACGCATGGc                                                                                                                      >  1:137480/1‑150 (MQ=255)

                                 gATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGc                                                                                                                      >  1:1569341/1‑150 (MQ=255)

                                 gATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGc                                                                                                                      >  2:1329315/1‑150 (MQ=255)

                                 gATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGc                                                                                                                      >  2:733431/1‑150 (MQ=255)

                                 gATTTTTCTACTGACGGGATGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGAACGACAGCCCAATGCAAGAAAAGTAAAGCATGGc                                                                                                                      >  1:244435/1‑150 (MQ=255)

                                 gATTTTTCTACTGACCGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGc                                                                                                                      <  2:453865/150‑1 (MQ=255)

                                      ttCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAAt                                                                                                                 <  1:1329315/150‑1 (MQ=255)

                                            tGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTg                                                                                                           <  1:553968/150‑1 (MQ=255)

                                              aCGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTTGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACACGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAg                                                                                                         <  1:1543641/150‑1 (MQ=255)

                                              aCGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAg                                                                                                         <  1:1606704/150‑1 (MQ=255)

                                                gggCTGATGAGACGTTATCTCACTGGCGGTAATAATCAGTGGCACACTGTAGACTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTa                                                                                                       <  1:75130/150‑1 (MQ=255)

                                                  gCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAG‑CCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCg                                                                                                    <  2:533413/150‑1 (MQ=255)

                                                    tgatgaGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCgg                                                                                                   <  2:1168581/150‑1 (MQ=255)

                                                     gatgaGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGc                                                                                                  >  1:1014286/1‑150 (MQ=255)

                                                            cGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGAt                                                                                           >  2:1139240/1‑150 (MQ=255)

                                                              ttATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATAc                                                                                         <  2:1008575/150‑1 (MQ=255)

                                                              ttATGTCACTGG‑GGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATAcc                                                                                        <  1:1258101/150‑1 (MQ=255)

                                                                aTGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCa                                                                                       <  1:1692501/150‑1 (MQ=255)

                                                                 tGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCAt                                                                                      <  1:313528/150‑1 (MQ=255)

                                                                 tGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCAt                                                                                      >  2:527220/1‑150 (MQ=255)

                                                                   tCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCa                                                                                    <  1:1660100/150‑1 (MQ=255)

                                                                   tCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCa                                                                                    <  1:792349/150‑1 (MQ=255)

                                                                      cTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGc                                                                                 >  2:1568928/1‑150 (MQ=255)

                                                                         gCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTgg                                                                              <  2:1005170/150‑1 (MQ=255)

                                                                              aataatCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAg                                                                         <  2:640132/150‑1 (MQ=255)

                                                                               ataatCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGa                                                                        >  1:1665263/1‑150 (MQ=255)

                                                                                   tCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCGAGCTGGCCAAGAAAGt                                                                    <  1:1262747/150‑1 (MQ=255)

                                                                                     aGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTa                                                                  <  2:540167/150‑1 (MQ=255)

                                                                                       tGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGACGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTGATc                                                                >  2:1120314/1‑150 (MQ=255)

                                                                                        ggCACACTGTAGCCTCCCTGCACATGACCATAATGATGTACAGGATTGGCAGTGTCGCTGACCGAGTGCCCAAGGCCTGAAAAGTATAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATcc                                                               <  2:1511916/150‑1 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTTGCACTGTCGCTGACCGAAAGCCCAAGGCCAGAAAATTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATcaa                                                              >  2:1552256/1‑148 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGt                                                                    >  2:50031/1‑144 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGt                                                                    <  1:50031/144‑1 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCa                                                              <  2:719508/150‑1 (MQ=255)

                                                                                         gCACACTGTAGCCTCCCAGCACATGACCATAATGATGAACAGGTTTGGCACTGTCGCTGACCGACAGCCAAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGAGACCATCAAGCTGGCCAAGAAAGTTATCCa                                                              <  1:290892/150‑1 (MQ=255)

                                                                                            cacTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAATTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGtt                                                           >  2:136048/1‑150 (MQ=255)

                                                                                            cacTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGtt                                                           <  2:622638/150‑1 (MQ=255)

                                                                                              cTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTa                                                         >  1:981548/1‑150 (MQ=255)

                                                                                                  aGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTATAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCCGTGTACTGa                                                     >  2:1245284/1‑150 (MQ=255)

                                                                                                  aGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGa                                                     <  2:946227/150‑1 (MQ=255)

                                                                                                   gCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGATAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGTAGGATACCATCAAGCTGGCCTAGAAAGTTATCCAGTTTACTGAt                                                    <  2:747180/150‑1 (MQ=255)

                                                                                                   gCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGAt                                                    <  1:1355981/150‑1 (MQ=255)

                                                                                                   gCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGAt                                                    >  2:640110/1‑150 (MQ=255)

                                                                                                       cccTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTg                                                >  2:1284272/1‑150 (MQ=255)

                                                                                                       cccTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGATGACCGACAGCCCAACGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAACGTTATCCAGTTTACTGATGCTg                                                >  2:120548/1‑150 (MQ=255)

                                                                                                         cTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGc                                              >  2:91277/1‑150 (MQ=255)

                                                                                                           tcacaTTACCATAATGATGAACAGGTTTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAATAGTAAAGCATGGCGAAATGACGTGAGTACCGGAGAAGGATACCATCAAGCTGGCCAAGAATGTTATCCAGTTTACTGATGCTGGCGa                                            <  1:189856/149‑1 (MQ=255)

                                                                                                            cacaTGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAg                                           <  2:1370269/150‑1 (MQ=255)

                                                                                                             acaTGACCATAATGATGATCAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAATTTATCCAGTTTACTGATGCTGGCGAgg                                          >  2:230530/1‑150 (MQ=255)

                                                                                                             acaTGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGCgg                                          >  2:235568/1‑150 (MQ=255)

                                                                                                               aTGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTa                                        <  1:1688666/150‑1 (MQ=255)

                                                                                                                         tgatgaACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAAtt                              <  2:1642115/150‑1 (MQ=255)

                                                                                                                         tgatgaACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGGTTACTGCTGCGGGCGAGGTACCAGGCAAtt                              >  1:209401/1‑150 (MQ=255)

                                                                                                                               aCAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGACAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCgg                        <  2:1059790/150‑1 (MQ=255)

                                                                                                                               aCAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCgg                        <  1:1709396/150‑1 (MQ=255)

                                                                                                                               aCAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTAAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCgg                        <  1:143649/150‑1 (MQ=255)

                                                                                                                                   gATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGAt                    >  2:421639/1‑150 (MQ=255)

                                                                                                                                    ccttgggcTGTCGGTCAGCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATa                   >  2:1633086/8‑150 (MQ=255)

                                                                                                                                     ttGGCACTGTCGCTGCCCGACAGCCCACGGCCAGAAAAGTAACGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCAGCACGGGGGCCTAGAAATTTATCCAGTTGACGGATGGTGGCGCGGTACCCGGCATTTTGGCggggggcg                  >  2:848094/1‑146 (MQ=255)

                                                                                                                                     ttGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATAc                  >  1:1352714/1‑150 (MQ=255)

                                                                                                                                     cttgggcTGTCGGTCAGCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATAc                  >  1:577181/7‑150 (MQ=255)

                                                                                                                                       ggCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTg                <  2:1014286/150‑1 (MQ=255)

                                                                                                                                       ggCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTg                >  2:1275466/1‑150 (MQ=255)

                                                                                                                                       ggCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTg                >  2:1694457/1‑150 (MQ=255)

                                                                                                                                        gCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGc               <  1:733431/150‑1 (MQ=255)

                                                                                                                                              tCGCTGACCGACATCCCAAGGCCAGAAAAGTAAAGCATGGCGAACTGACGTGAGTAAGGGCGAAGGATACCATCAAGCTGGCCAAGAAATTTATCCAGTTTACTGATGCTGGCCAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAgg         <  2:244435/150‑1 (MQ=255)

                                                                                                                                                   gACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAatt    >  1:142902/1‑150 (MQ=255)

                                                                                                                                                    aCCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAatta   <  1:759465/150‑1 (MQ=255)

                                                                                                                                                    aCCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAatta   >  2:1720683/1‑150 (MQ=255)

                                                                                                                                                     ccGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGCTACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAattat  >  1:938736/1‑150 (MQ=255)

                                                                                                                                                     ccGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAattat  >  2:1328464/1‑150 (MQ=255)

CCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTAT  >  NC_000913/4499740‑4500038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`