Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,517,558 C→A 6.7% E54* (GAA→TAA) ‡ fecR ← anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,517,5580CA 6.7% 366.2 / 12.8 120E54* (GAA→TAA) ‡fecRanti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
Reads supporting (aligned to +/- strand):  ref base C (62/50);  new base A (3/5);  total (65/55)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

Only 100 of 121 total aligned reads displayed.

GGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGG  >  NC_000913/4517409‑4517701
                                                                                                                                                     |                                                                                                                                               
ggTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTc                                                                                                                                                 <  2:735470/150‑1 (MQ=255)
  tttCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCaa                                                                                                                                               >  1:1217854/1‑150 (MQ=255)
  tttCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCaa                                                                                                                                               <  2:1259450/150‑1 (MQ=255)
    tCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAAGTTTTCAAcc                                                                                                                                             <  2:1279341/150‑1 (MQ=255)
     ccGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCCAccg                                                                                                                                            >  1:756048/1‑149 (MQ=255)
      cGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACctg                                                                                                                                           >  2:1608055/1‑150 (MQ=255)
        ccTGCCAGAGCTGCCAGCCCCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGCCGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACctgct                                                                                                                                         <  1:1301168/149‑1 (MQ=255)
         cagCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGATGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTAGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTGAAAACctgcgc                                                                                                                                        <  1:817875/148‑3 (MQ=255)
         cTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACctgctg                                                                                                                                        <  2:611579/150‑1 (MQ=255)
         cTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTGAAAACctgcgc                                                                                                                                        <  2:1368410/150‑3 (MQ=255)
          tGCCAGAGCTGCCAGCCTCCGCCCGCGCCGCGCAACAGCAGAAATCCATTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTGAAAACctgcgca                                                                                                                                       <  2:1051245/150‑4 (MQ=255)
          tGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCtgctgc                                                                                                                                       >  1:240384/1‑150 (MQ=255)
          tGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTGAAAACctgcgca                                                                                                                                       <  1:1153067/150‑4 (MQ=255)
          tGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTGAAAACctgcgca                                                                                                                                       <  1:1147229/150‑4 (MQ=255)
           gCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGcc                                                                                                                                      <  2:428874/150‑1 (MQ=255)
            ccAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCa                                                                                                                                     >  1:526563/1‑150 (MQ=255)
                   tGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCAc                                                                                                                              >  1:432618/1‑150 (MQ=255)
                    gCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACt                                                                                                                             >  1:462009/1‑150 (MQ=255)
                      cAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTgg                                                                                                                           >  1:1594939/1‑150 (MQ=255)
                        gCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGtt                                                                                                                         <  2:646804/150‑1 (MQ=255)
                         ccTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGttt                                                                                                                        <  1:602750/150‑2 (MQ=255)
                          cTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGc                                                                                                                                  <  2:681586/139‑1 (MQ=255)
                          cTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGc                                                                                                                                  >  1:681586/1‑139 (MQ=255)
                                cAGCGCCGAGCAACAGCAGTATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgatc                                                                                                                >  2:1521499/1‑150 (MQ=255)
                                 aGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgatc                                                                                                                <  2:1130267/150‑1 (MQ=255)
                                 aGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgatc                                                                                                                >  2:1225965/1‑150 (MQ=255)
                                  gcgcCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTatcctgatcc                                                                                                               <  1:1677868/150‑1 (MQ=255)
                                     ccGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGGTATCCTGATCCTGt                                                                                                            >  1:501225/1‑150 (MQ=255)
                                       gAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTc                                                                                                          >  2:542229/1‑150 (MQ=255)
                                       gAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTc                                                                                                          <  2:1262075/150‑1 (MQ=255)
                                         gCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTTAt                                                                                                        <  1:1601798/150‑1 (MQ=255)
                                            aCAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATAcc                                                                                                     <  2:944006/150‑1 (MQ=255)
                                            aCAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATAcc                                                                                                     <  2:231266/150‑1 (MQ=255)
                                              agcagTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCAc                                                                                                   >  2:1439141/1‑150 (MQ=255)
                                               gcagTATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGCTCCTGTTCATACCACTg                                                                                                 >  2:930391/1‑150 (MQ=255)
                                                cagTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTg                                                                                                 <  2:1611824/150‑1 (MQ=255)
                                                  gTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGtt                                                                                               >  1:1496319/1‑150 (MQ=255)
                                                    aaTCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCCCTGGGTATCCTGATCCTGTTCATACCACTGTTGc                                                                                             >  1:113443/1‑150 (MQ=255)
                                                     aTCCTTTCATCACGTGACGGCTGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCTGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGcc                                                                                            <  1:1265733/150‑1 (MQ=255)
                                                        cTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCCCTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAgc                                                                                         >  2:1175401/1‑150 (MQ=255)
                                                        cTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAgc                                                                                         <  1:1225965/150‑1 (MQ=255)
                                                            catcaCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAgcgcgc                                                                                     >  2:702368/1‑150 (MQ=255)
                                                            catcaCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAgcgcgc                                                                                     >  1:1119268/1‑150 (MQ=255)
                                                              tcaCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCtt                                                                                   >  2:608560/1‑150 (MQ=255)
                                                               caCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGCTCCTGTTCATACCACTGTTGCCAGCGCGCTTc                                                                                  >  1:104521/1‑150 (MQ=255)
                                                                aCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTcc                                                                                 >  1:1175807/1‑150 (MQ=255)
                                                                aCGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTcc                                                                                 <  1:552812/150‑1 (MQ=255)
                                                                 cGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCt                                                                                >  2:469466/1‑150 (MQ=255)
                                                                          gggTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGc                                                                       <  2:1319419/150‑1 (MQ=255)
                                                                            gTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTg                                                                     <  2:1110252/150‑1 (MQ=255)
                                                                               aGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGAcg                                                                  >  1:949507/1‑150 (MQ=255)
                                                                                ggCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCACCCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCCGCGCGCTTCCTGTTGTGGGCTGAcgc                                                                 >  1:1485520/1‑150 (MQ=255)
                                                                                ggCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGAcgc                                                                 >  1:1231045/1‑150 (MQ=255)
                                                                                   gggTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCGGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCGGACGCGCt                                                              <  1:1316244/150‑1 (MQ=255)
                                                                                      tATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGAGCTGACGCGCTcgc                                                           <  2:840206/150‑1 (MQ=255)
                                                                                         cGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGGTCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTcgccgc                                                        >  1:458614/1‑150 (MQ=255)
                                                                                           tGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCtt                                                      <  1:1267460/150‑1 (MQ=255)
                                                                                            gCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCAAACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTa                                                     >  2:520469/1‑150 (MQ=255)
                                                                                                  ccccGCTGGCAACGTCGCCAGGCACACCGCCAAGCGGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCAGATCCTGTTCATACCACTGTTGCCCGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACgg                                               <  2:171113/150‑1 (MQ=255)
                                                                                                  cccGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACgg                                               >  2:946818/1‑150 (MQ=255)
                                                                                                   ccGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGATGTGGGCTGACGCGCTCGCCGCTTAGCACGGc                                              <  2:174022/150‑1 (MQ=255)
                                                                                                     ggCTGGCAACGGCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTGCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATCCCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCGTAGCACGGCAt                                            <  2:79812/150‑1 (MQ=255)
                                                                                                     gccTGGCGACGTTGCCAGGCACACCTCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCAt                                            >  2:292915/3‑150 (MQ=255)
                                                                                                      gCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTcc                                                                                 <  1:213944/112‑1 (MQ=255)
                                                                                                      gCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTcc                                                                                 >  2:213944/1‑112 (MQ=255)
                                                                                                      gCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCACTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATa                                           >  1:330965/1‑150 (MQ=255)
                                                                                                       cTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCAGCTTAGCACGGCATAc                                          >  2:1218483/1‑150 (MQ=255)
                                                                                                        tGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATAcc                                         <  2:526563/150‑1 (MQ=255)
                                                                                                        tGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCa                                        <  2:571471/150‑1 (MQ=255)
                                                                                                         ggCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGTGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCa                                        >  1:588848/1‑150 (MQ=255)
                                                                                                         ggCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCa                                        >  1:1143098/1‑150 (MQ=255)
                                                                                                           cAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGt                                      >  2:430400/1‑150 (MQ=255)
                                                                                                           cAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGt                                      >  1:1497803/1‑150 (MQ=255)
                                                                                                            aaCGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGATCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTg                                     <  2:161001/150‑1 (MQ=255)
                                                                                                             aCGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTgg                                    <  1:927839/150‑1 (MQ=255)
                                                                                                                  gCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGCAAGc                               >  2:322411/1‑150 (MQ=255)
                                                                                                                   ccAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCt                              >  1:1402651/1‑150 (MQ=255)
                                                                                                                    cAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTg                             <  1:57515/150‑1 (MQ=255)
                                                                                                                    cAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTg                             >  1:1391140/1‑150 (MQ=255)
                                                                                                                      ggCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGaa                           >  1:1124560/1‑150 (MQ=255)
                                                                                                                      ggCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGaa                           >  2:525854/1‑150 (MQ=255)
                                                                                                                       gCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAAc                          <  1:1533972/150‑1 (MQ=255)
                                                                                                                       gCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAAc                          <  1:570028/150‑1 (MQ=255)
                                                                                                                          cacCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCa                       <  1:430400/150‑1 (MQ=255)
                                                                                                                          cacCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCa                       >  1:515899/1‑150 (MQ=255)
                                                                                                                           acCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAg                      <  1:522621/150‑1 (MQ=255)
                                                                                                                           acCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAg                      >  1:1416817/1‑150 (MQ=255)
                                                                                                                           acCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCAAGTCCCCCTGGTTATCCTGATCCTGTTCATACCACTTTTGCCAGCGCGCTTCCGGTTCTGGGCTGACGCGCTATCCGCTTAGCACGGCATACCAGTTGGAAGCTGAACGCAg                      >  1:1034064/1‑150 (MQ=255)
                                                                                                                                    cTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGCcg             >  1:1547252/1‑150 (MQ=255)
                                                                                                                                     tGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGAcgg            >  1:474715/1‑150 (MQ=255)
                                                                                                                                      ggTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGAcggc           >  2:761732/1‑150 (MQ=255)
                                                                                                                                      ctggcagcAGGTTGAAAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGAcggc           >  1:1324107/7‑150 (MQ=255)
                                                                                                                                      ctggcagcAGGTTGAAAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGAcggc           >  1:258584/7‑150 (MQ=255)
                                                                                                                                         tGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCggg        >  1:975653/1‑150 (MQ=255)
                                                                                                                                         tGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCggg        <  2:761946/150‑1 (MQ=255)
                                                                                                                                          gcgcAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGa       >  2:1562940/1‑150 (MQ=255)
                                                                                                                                               ggTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCgg  <  2:1594939/150‑1 (MQ=255)
                                                                                                                                               ggTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCgg  <  2:38410/150‑1 (MQ=255)
                                                                                                                                               ggTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCgg  >  1:1145168/1‑150 (MQ=255)
                                                                                                                                               ggTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCgg  <  1:1608055/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
GGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTGGTTATCCTGATCCTGTTCATACCACTGTTGCCAGCGCGCTTCCTGTTGTGGGCTGACGCGCTCGCCGCTTAGCACGGCATACCAGTGGGAAGCTGAACGCAGCGCCTGACGGCGGGAATCGG  >  NC_000913/4517409‑4517701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: