Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,529,498 A→G 6.8% V249A (GTT→GCT) ‡ sgcC ← putative PTS system EIIC permease component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,529,4980AG 6.8% 332.8 / 10.9 118V249A (GTT→GCT) ‡sgcCputative PTS system EIIC permease component
Reads supporting (aligned to +/- strand):  ref base A (61/48);  new base G (3/5);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01

Only 100 of 118 total aligned reads displayed.

CCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTCCCTTCA  >  NC_000913/4529350‑4529645
                                                                                                                                                    |                                                                                                                                                   
ccccAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAAc                                                                                                                                                    >  2:1017917/1‑150 (MQ=255)
  ccAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACgg                                                                                                                                                  >  2:1312838/1‑150 (MQ=255)
   cAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGt                                                                                                                                                 <  2:72150/150‑1 (MQ=255)
     gCGTCACAGCAGTATCCAGACCGATATACACTTCACGACCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCATCAGTCCATCAAAAAACAGGCGGATAAAGCCCGGGAACAACACCATGATTGCCGAAACGGaaa                                                                                                                                               >  2:1619382/1‑150 (MQ=255)
       gTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatc                                                                                                                                             >  2:1030374/1‑150 (MQ=255)
         cacaGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAatcat                                                                                                                                           >  2:790748/1‑150 (MQ=255)
           cagcatTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGTGCAGCAGGCCCTCATCAATCAGGCGGATCATGCGCGGGAACAACACCATGATTACCGTTGCGGCAatcatgg                                                                                                                                         <  2:686475/150‑3 (MQ=255)
            agcagTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTACCGTTGCGGCAatcatggt                                                                                                                                        <  1:589691/150‑4 (MQ=255)
               agTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTACCGTTGCGGCAatcatggtgtt                                                                                                                                     <  2:1591555/150‑7 (MQ=255)
                ttATCCATACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTg                                                                                                                                    <  2:743204/149‑1 (MQ=255)
                 tATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTgg                                                                                                                                   <  2:1222069/150‑1 (MQ=255)
                  aTCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCACCGGTAATCATCAGACTGgc                                                                                                                                  >  2:57826/1‑150 (MQ=255)
                   accAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGATATGGGCATCAGGCCCGCATCAATCAGGCGGATCATGCGCGGGAACAACTCCATTATTGCCGCAACGGTAATCATCAGACTGgcg                                                                                                                                 <  1:382237/149‑1 (MQ=255)
                    ccAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgcgc                                                                                                                                >  1:1308587/1‑150 (MQ=255)
                    ccAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgcgc                                                                                                                                >  1:466732/1‑150 (MQ=255)
                    ccAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGgcgc                                                                                                                                >  1:803649/1‑150 (MQ=255)
                       gACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAAc                                                                                                                             >  2:654304/1‑150 (MQ=255)
                       gACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAAc                                                                                                                             >  2:949132/1‑150 (MQ=255)
                       gACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTACCGTTGCGGCAatcatg                                                                                                                                          >  1:1341984/1‑136 (MQ=255)
                       gACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTACCGTTGCGGCAatcatg                                                                                                                                          <  2:1341984/137‑2 (MQ=255)
                           gATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCttt                                                                                                                         <  2:803649/150‑1 (MQ=255)
                            atatACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTa                                                                                                                        <  2:156110/150‑1 (MQ=255)
                                acacTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGc                                                                                                                    >  1:554604/1‑150 (MQ=255)
                                    ttCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTc                                                                                                                <  1:790748/150‑1 (MQ=255)
                                    ttCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTAAAGCCTTCa                                                                                                               >  1:182603/1‑150 (MQ=255)
                                     tCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCa                                                                                                               <  2:301513/150‑1 (MQ=255)
                                      cACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGCTCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCAc                                                                                                              >  1:351021/1‑150 (MQ=255)
                                      cACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCAc                                                                                                              >  1:397835/1‑150 (MQ=255)
                                           ccTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgcc                                                                                                         >  2:916120/1‑150 (MQ=255)
                                           ccTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgcc                                                                                                         >  1:566015/1‑150 (MQ=255)
                                           ccTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgcc                                                                                                         <  1:57826/150‑1 (MQ=255)
                                            cTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAACGCCTTCACCAgccg                                                                                                        >  1:1015372/1‑150 (MQ=255)
                                            cTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgccg                                                                                                        <  1:1030374/150‑1 (MQ=255)
                                            cTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgccg                                                                                                        >  2:446834/1‑150 (MQ=255)
                                             tttGAAATAATTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGAAGCAGGCCCTCAACACTCAGGCGGATCAAGCGCGGGAACCAAACCATGATTGCCGCAACGGTAATAATCAGACTGCCGCAACCTTTAAAGCCTTCACCAgcctc                                                                                                       >  1:1533702/1‑148 (MQ=255)
                                              ttGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAgccgcc                                                                                                      >  2:1036558/1‑150 (MQ=255)
                                               tGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCa                                                                                                     <  2:172273/150‑1 (MQ=255)
                                                gAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAg                                                                                                    <  1:26370/150‑1 (MQ=255)
                                                 aaaTATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAgg                                                                                                   <  1:567357/150‑1 (MQ=255)
                                                 aaaTATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAgg                                                                                                   <  2:566015/150‑1 (MQ=255)
                                                   atatTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGcc                                                                                                 <  2:554604/150‑1 (MQ=255)
                                                     atTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCaa                                                                                               >  1:1678279/1‑150 (MQ=255)
                                                       ttCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCaaaa                                                                                             <  1:1017917/150‑1 (MQ=255)
                                                       atCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCATCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTAGCGCAACCTTAAAAGCCTTAAACAGCCGCCAGGCCaaaa                                                                                             >  1:413698/2‑150 (MQ=255)
                                                          tGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCCGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGGAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATc                                                                                          >  1:1545921/1‑150 (MQ=255)
                                                          tGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATc                                                                                          <  2:359546/150‑1 (MQ=255)
                                                           ggAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCa                                                                                         >  1:1396150/1‑150 (MQ=255)
                                                            gaagaaCTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAg                                                                                        <  2:809135/150‑1 (MQ=255)
                                                                aaCTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCg                                                                                    <  2:1234071/150‑1 (MQ=255)
                                                                aaCTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCg                                                                                    <  2:1606937/150‑1 (MQ=255)
                                                                 aCTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGa                                                                                   >  1:467943/1‑150 (MQ=255)
                                                                  cTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACACCACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCGACCTTTAACGCCGTCACCCGCCGCCAGGCCCAAACTCAGCCCGAg                                                                                  >  2:225222/1‑150 (MQ=255)
                                                                  cTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAg                                                                                  <  1:95478/150‑1 (MQ=255)
                                                                         gCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACg                                                                           >  1:312139/1‑150 (MQ=255)
                                                                              gTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGAt                                                                      >  2:1421852/1‑150 (MQ=255)
                                                                                cGGAGCTGGGCAGCAGGCCCTCAACAATCAGGCGGATCATTCGCTGGAACAACACCATGATTGACTCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATaa                                                                    <  2:253333/150‑1 (MQ=255)
                                                                                cGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGCCCGAGCACTACGCCGATaa                                                                    >  1:1612219/1‑150 (MQ=255)
                                                                                    gaTGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAAACCGAGCACTACGCCGATAATCAc                                                                >  2:1035459/1‑150 (MQ=255)
                                                                                        ggCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGa                                                            <  1:446834/150‑1 (MQ=255)
                                                                                              agGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCACCCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAACGCAGACCGCGCACTTCGCCGATAATCACCGGATCGCCg                                                      >  1:868564/1‑150 (MQ=255)
                                                                                               ggCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGCCTGCCGCAACATTTAACGCCTGCCCCAGCCGCCAGGCCAAAATTCAGCCCGAGCAGTACGCCGATAATCACCGGAGCCCCgc                                                     >  1:678135/1‑149 (MQ=255)
                                                                                                gCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGAc                                                    >  2:1710794/1‑150 (MQ=255)
                                                                                                 cccTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGAcc                                                   >  1:1268669/1‑150 (MQ=255)
                                                                                                     caacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGc                                               <  1:1312838/150‑1 (MQ=255)
                                                                                                      aacaaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGcc                                              <  2:1250221/150‑1 (MQ=255)
                                                                                                        caaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGt                                            >  1:1197367/1‑150 (MQ=255)
                                                                                                        caaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGt                                            <  2:397999/150‑1 (MQ=255)
                                                                                                         aaTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTa                                           <  2:312139/150‑1 (MQ=255)
                                                                                                          aTCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAg                                          >  1:1183290/1‑150 (MQ=255)
                                                                                                           tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGc                                         <  2:764851/150‑1 (MQ=255)
                                                                                                           tCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCATTCACAAGCCGACAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGACGTAGc                                         >  1:1045884/1‑150 (MQ=255)
                                                                                                              ggCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCTGTAGCGtt                                      >  1:1583757/1‑150 (MQ=255)
                                                                                                              ggCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGtt                                      >  2:936516/1‑150 (MQ=255)
                                                                                                                cGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTc                                    <  1:1670225/150‑1 (MQ=255)
                                                                                                                cGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTc                                    >  2:254677/1‑150 (MQ=255)
                                                                                                                 ggATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCt                                   <  2:1352163/150‑1 (MQ=255)
                                                                                                                   aTCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCTCCGGATCGCCGACCATGCCGTAGCGTTTCTgg                                 <  1:321284/150‑1 (MQ=255)
                                                                                                                    tCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGa                                <  1:1036558/150‑1 (MQ=255)
                                                                                                                     cATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGAt                               <  2:1183290/150‑1 (MQ=255)
                                                                                                                      aTGCGCGGGAACAACACCATGATTGCCGCAGCGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAAGCACCGGATCGCCGCCCATGCCGTAGCGGTTCGGGAtc                              >  2:855310/1‑150 (MQ=255)
                                                                                                                       tGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGAtct                             <  1:654304/150‑1 (MQ=255)
                                                                                                                             ggAACAACACCATGATTGCCGCTACGGTTATCATCAGACTGTCGCAACCTTTATAGCCTTCAGCAGCCGCCAGGCCAAAAATCAGACCGAGCTCTACGCCGATAATCTCCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAg                       <  1:1619382/150‑1 (MQ=255)
                                                                                                                             ggAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGCCCATGCCGTAGCGTTTCTGGATCTCCTGAg                       >  1:1183348/1‑150 (MQ=255)
                                                                                                                             ggAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAg                       >  2:247249/1‑150 (MQ=255)
                                                                                                                             ggAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCAGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAg                       >  1:1189562/1‑150 (MQ=255)
                                                                                                                             ggAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCAGCCAGGCCAAAAATCAGACCGAGCACTACGCCTATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAg                       <  2:555644/150‑1 (MQ=255)
                                                                                                                                acaacaCCATGATTGCCGCAACGGTAATCATCTGACTGGAGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCAt                    >  2:212004/1‑150 (MQ=255)
                                                                                                                                  aacaCCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCg                  >  1:1618788/1‑150 (MQ=255)
                                                                                                                                   acacCATGATTGCCGCAACGGTAATCATCAGAAAGGCGCAACCTTTAAAGCCTTCAAAATCCAAAAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGa                 >  2:1074414/1‑150 (MQ=255)
                                                                                                                                    cacCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGCCCATGCCGTAGCGTTTCTGGCTCTCCTGAGCATCGat                >  1:1129358/1‑150 (MQ=255)
                                                                                                                                    cacCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGat                >  1:1135288/1‑150 (MQ=255)
                                                                                                                                       cATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATAtt             <  2:1308587/150‑1 (MQ=255)
                                                                                                                                        aTGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTg            <  1:254677/150‑1 (MQ=255)
                                                                                                                                         tGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGc           >  1:888094/1‑150 (MQ=255)
                                                                                                                                             tGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGAGCTCCTGAGCATCGATATTGCGTcc       <  1:936516/150‑1 (MQ=255)
                                                                                                                                              gCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCCGGCCAAAACTCAGACCGAGAACTACGCCGATAATCACCGGATCGCCGAGCATGCGGGAGCGTTTCTGGCTCTCCTGAGCATCGAGATTGCGTccc      >  1:80836/1‑150 (MQ=255)
                                                                                                                                               ccGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTCCCt     >  1:362130/1‑150 (MQ=255)
                                                                                                                                                 gCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTATGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTcc       >  2:1107100/1‑146 (MQ=255)
                                                                                                                                                 gCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTATGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTcc       <  1:1107100/146‑1 (MQ=255)
                                                                                                                                                  cAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTCCCTTCa  <  2:467943/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCCCAGCGTCACAGCAGTATCCAGACCGATATACACTTCACGTCCTTTGAAATATTTCTGGAAGAACTTTCTTGCACCGTCGGAGATGGGCAGCAGGCCCTCAACAATCAGGCGGATCATGCGCGGGAACAACACCATGATTGCCGCAACGGTAATCATCAGACTGGCGCAACCTTTAAAGCCTTCACCAGCCGCCAGGCCAAAAATCAGACCGAGCACTACGCCGATAATCACCGGATCGCCGACCATGCCGTAGCGTTTCTGGATCTCCTGAGCATCGATATTGCGTCCCTTCA  >  NC_000913/4529350‑4529645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: