Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,591,498 T→A 7.0% intergenic (‑219/‑159) yjiY ← / → tsr putative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,591,4980TA 7.0% 367.7 / 7.9 126intergenic (‑219/‑159)yjiY/tsrputative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor
Reads supporting (aligned to +/- strand):  ref base T (58/58);  new base A (7/3);  total (65/61)
Fisher's exact test for biased strand distribution p-value = 3.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GTAAATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAA  >  NC_000913/4591349‑4591647
                                                                                                                                                     |                                                                                                                                                     
gTAAATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGAtt                                                                                                                                                       >  2:677403/1‑150 (MQ=255)
  aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                     <  2:530557/150‑1 (MQ=255)
  aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                     <  2:523768/150‑1 (MQ=255)
  aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                     >  2:1213544/1‑150 (MQ=255)
  aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                     <  1:744758/150‑1 (MQ=255)
    aTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGCGCGGCAAAGGCTTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                                   >  2:316024/1‑150 (MQ=255)
    aTTGACCGCTATCCTGTAGTGATGGTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCTTCGTTTAAGTGACGATAAATATGTGATACata                                                                                                                                                   >  1:136928/1‑150 (MQ=255)
    aTTGACCGCCATCCTGTCGTGATGTTGCCAGAGTTACGCGCGCTTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                                   <  2:861576/150‑1 (MQ=255)
        aCCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATcac                                                                                                                                               <  1:671070/150‑1 (MQ=255)
        aCCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATcac                                                                                                                                               <  1:665061/150‑1 (MQ=255)
          cGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACat                                                                                                                                             >  2:1566200/1‑150 (MQ=255)
            cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACatat                                                                                                                                           <  1:1065661/150‑1 (MQ=255)
            cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACatat                                                                                                                                           <  2:1015895/150‑1 (MQ=255)
             tATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATAtt                                                                                                                                          >  2:108495/1‑150 (MQ=255)
             tATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATAtt                                                                                                                                          <  2:1171755/150‑1 (MQ=255)
                 cTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATATTtatc                                                                                                                                      >  1:377845/1‑149 (MQ=255)
                  tGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATATTtatcg                                                                                                                                     <  2:377845/150‑3 (MQ=255)
                     aGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATtgt                                                                                                                                  <  2:1082320/150‑1 (MQ=255)
                        gATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGaa                                                                                                                               >  2:1666716/1‑150 (MQ=255)
                          tGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCATAGGTGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTTTATTGTGaata                                                                                                                             >  2:1144133/1‑150 (MQ=255)
                             tGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAAtt                                                                                                                          <  2:960860/150‑1 (MQ=255)
                                 agagTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATg                                                                                                                      <  2:66617/150‑1 (MQ=255)
                                        cgcgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGGTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAaataa                                                                                                               >  1:39196/1‑150 (MQ=255)
                                          ctcgcgTAAAGTATGGTTTATAATTTAGTTTTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGGCTAAGGCTTCGTTTTATTGACGATAAATATTTGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATATTg                                                                                                             <  2:767761/148‑1 (MQ=255)
                                          cgggcttAAAGTAAGGTAAATAACTGTGTGTTTATTTTTGGTTTGTACGCGGTATGTTTTGGGGTTTTGCGGAAAAGGCGTCGTTTATTTTACTATAAATTTGTTATTCATATCAAATATTTATTTTGTTAATAATTTATTTAAATAATg                                                                                                             <  2:1121960/144‑1 (MQ=255)
                                          cgcgcgTAAAGTAATGTAAATAACTGAGTGGTTATTTTAGGGATCTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                             <  2:901638/150‑1 (MQ=255)
                                          cgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                             >  1:1391190/1‑150 (MQ=255)
                                                  aaGTAAGGTAAATAACTTAGTGGTTATTTTAGGGATGTAATCTTTAAGTTTTGCGGTTGAGCGTCAAAGTCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                     <  1:1147975/150‑1 (MQ=255)
                                                  aaGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                     <  2:1317105/150‑1 (MQ=255)
                                                   aGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaa                                                                                                    >  2:587/1‑150 (MQ=255)
                                                    gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                                   >  2:1498293/1‑150 (MQ=255)
                                                    gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                                   >  1:14437/1‑150 (MQ=255)
                                                     tAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAAt                                                                                                  <  1:1420636/150‑1 (MQ=255)
                                                        ggTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCttttt                                                                                                      <  1:891294/143‑1 (MQ=255)
                                                        ggTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCttttt                                                                                                      >  2:891294/1‑143 (MQ=255)
                                                         gTAAATAACTGAGTGGTTATTTTATGGATGTAAGCGGTCAGTTTTGCGGTTGAGCATCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCag                                                                                              <  1:1354192/150‑1 (MQ=255)
                                                         gTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCag                                                                                              >  1:909708/1‑150 (MQ=255)
                                                           aaaTAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCagat                                                                                            >  1:670915/1‑149 (MQ=255)
                                                              tAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtgt                                                                                         >  2:103080/1‑150 (MQ=255)
                                                              tAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtgt                                                                                         >  1:373282/1‑150 (MQ=255)
                                                                aCTGAGTGGTTATTTTAGGGATGTAAGCTGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGa                                                                                       <  1:493373/150‑1 (MQ=255)
                                                                 cTGAGTGGTTATTTTAGGGATGTAAGATGTCAGTTTTGCGGTTGAGCGGAAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGAATTTTTAAATTCAGAGTGTGaa                                                                                      >  2:1113960/1‑150 (MQ=255)
                                                                 atGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGaa                                                                                      <  1:933891/149‑1 (MQ=255)
                                                                    aGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATaa                                                                                   <  1:108495/150‑1 (MQ=255)
                                                                    aGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATaa                                                                                   >  2:1662658/1‑150 (MQ=255)
                                                                       ggTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAAt                                                                                <  1:1181214/150‑1 (MQ=255)
                                                                        gTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATAATGTCTTTTTAAATTCAGAGTGTGAATAAAAtt                                                                               >  1:258471/1‑150 (MQ=255)
                                                                          tATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTTAGCGGCAAAGGCTTCGTTTAAGTGACGATAATTATGTGATTCATATGACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCTGTGTGTGAATAAAATTCa                                                                             <  2:511152/150‑1 (MQ=255)
                                                                           aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAATTAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCaa                                                                            >  1:111818/1‑149 (MQ=255)
                                                                           aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCAc                                                                            <  1:1404380/150‑1 (MQ=255)
                                                                              ttAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATTTTATTCAcact                                                                         <  2:191422/150‑4 (MQ=255)
                                                                               tAGGGATGTAAGCGGTCAGTTTTTCGGTTGAGCGGCAAAGGGGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGGTTTTTAAATTCAGAGTGTGAATTTTATTCAcactc                                                                        <  2:96352/150‑5 (MQ=255)
                                                                               tAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATCGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCgg                                                                        <  1:1426397/150‑1 (MQ=255)
                                                                                 tggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGTCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                      <  2:803409/149‑1 (MQ=255)
                                                                                 gggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                      >  2:687947/1‑150 (MQ=255)
                                                                                  ggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATAACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGAGAATAAAATTCACTCGGCGt                                                                     >  1:201767/1‑150 (MQ=255)
                                                                                      gTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAAtc                                                                 <  1:48337/150‑1 (MQ=255)
                                                                                             gTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCgg                                                          >  1:794707/1‑150 (MQ=255)
                                                                                             gTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTAGGCGTAAtctcagagg                                                          >  1:158352/1‑145 (MQ=255)
                                                                                               cAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGTGTAATCTCCGCGGGa                                                        >  2:765078/1‑150 (MQ=255)
                                                                                               cAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGa                                                        >  2:1216311/1‑150 (MQ=255)
                                                                                                aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                     >  2:756519/1‑120 (MQ=255)
                                                                                                aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGat                                                       >  1:1620869/1‑150 (MQ=255)
                                                                                                 gTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGata                                                      >  2:28354/1‑150 (MQ=255)
                                                                                                        ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATTGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                               >  2:964399/1‑150 (MQ=255)
                                                                                                                 gCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcc                                      <  1:687947/150‑1 (MQ=255)
                                                                                                                        cgtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAAGAATGGCTTTTTAAATTCAGAGTGTGATTAAAATTCACTCGGCTTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCAAg                               >  1:309747/1‑150 (MQ=255)
                                                                                                                         gtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAgg                              <  2:1620869/150‑1 (MQ=255)
                                                                                                                         gtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATATCCGCGGGATATTCATAAAGTTTTTACTTTCCAgg                              >  1:27587/1‑150 (MQ=255)
                                                                                                                          tcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGc                             <  1:1144133/150‑1 (MQ=255)
                                                                                                                           cgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGcc                            <  1:73732/150‑1 (MQ=255)
                                                                                                                            gtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCg                           >  2:1416017/1‑150 (MQ=255)
                                                                                                                                  gTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCTAAAATc                     >  1:1491457/1‑150 (MQ=255)
                                                                                                                                   acaataTAAATATGTGATATGAATCGCATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCt                    >  2:155429/6‑150 (MQ=255)
                                                                                                                                    gACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCtt                   >  1:922984/1‑150 (MQ=255)
                                                                                                                                    gACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCtt                   >  1:679210/1‑150 (MQ=255)
                                                                                                                                     aataTAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATCTAAATAATGGCTTTTTAAATTCATAGTGTTAATAAAATTCACTCGGAGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCAAGGCCGAAAATCTTg                  >  1:1133239/4‑150 (MQ=255)
                                                                                                                                     aCGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  <  1:103080/150‑1 (MQ=255)
                                                                                                                                     aCGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  <  2:373282/150‑1 (MQ=255)
                                                                                                                                     aCGATAAATATGGGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  <  1:1487437/150‑1 (MQ=255)
                                                                                                                                      cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc                 >  2:912477/1‑150 (MQ=255)
                                                                                                                                      cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTATCCTTTCCAGGCCGAAAATCTTGc                 >  2:1217242/1‑150 (MQ=255)
                                                                                                                                      agATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc                 <  1:181198/149‑1 (MQ=255)
                                                                                                                                       gATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCa                >  1:624814/1‑150 (MQ=255)
                                                                                                                                        aTAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  >  1:1137615/1‑147 (MQ=255)
                                                                                                                                         tAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATc              <  2:136928/150‑1 (MQ=255)
                                                                                                                                         tAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATc              >  1:382011/1‑150 (MQ=255)
                                                                                                                                          aaaTATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGCAAATCTTGCATCg             >  2:305670/1‑150 (MQ=255)
                                                                                                                                           aaTATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCgg            <  2:1144073/150‑1 (MQ=255)
                                                                                                                                           aaTATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTAAATCGGCTTAATCTCAGCTGTATATTCATAAAGTTTTTCCTTTCAATGCCGAAAATCTTGAATCgg            >  1:248640/1‑150 (MQ=255)
                                                                                                                                             tatGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTc          >  2:1323904/1‑150 (MQ=255)
                                                                                                                                               tgtgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTAACTCGGCTTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCca        >  1:1112984/1‑150 (MQ=255)
                                                                                                                                                 tgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCcaca      <  1:1666716/150‑1 (MQ=255)
                                                                                                                                                 tgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCcaca      <  2:909708/150‑1 (MQ=255)
                                                                                                                                                  gATTCATATCACATTTTTATATTGTTAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGGGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg     <  2:111818/150‑1 (MQ=255)
                                                                                                                                                  gATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg     <  2:322274/150‑1 (MQ=255)
                                                                                                                                                  gATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg     >  1:850833/1‑150 (MQ=255)
                                                                                                                                                    ttCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGa   <  1:28354/150‑1 (MQ=255)
                                                                                                                                                     tCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTGAGATTGTGATTAAAATTCACTCGGCGTAATATCCGGGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCTTCGGTCCACAGGaa  <  1:1323960/150‑1 (MQ=255)
                                                                                                                                                     tCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGaa  <  2:670915/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTAAATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAA  >  NC_000913/4591349‑4591647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: