Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,591,499 C→T 6.9% intergenic (‑220/‑158) yjiY ← / → tsr putative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,591,4990CT 6.9% 400.7 / 10.5 129intergenic (‑220/‑158)yjiY/tsrputative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor
Reads supporting (aligned to +/- strand):  ref base C (59/61);  new base T (6/3);  total (65/64)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01

Only 100 of 129 total aligned reads displayed.

AAATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAA  >  NC_000913/4591351‑4591647
                                                                                                                                                    |                                                                                                                                                    
aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                     <  1:744758/150‑1 (MQ=255)
aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                     >  2:1213544/1‑150 (MQ=255)
aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                     <  2:523768/150‑1 (MQ=255)
  aTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGCGCGGCAAAGGCTTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                                   >  2:316024/1‑150 (MQ=255)
  aTTGACCGCTATCCTGTAGTGATGGTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCTTCGTTTAAGTGACGATAAATATGTGATACata                                                                                                                                                   >  1:136928/1‑150 (MQ=255)
  aTTGACCGCCATCCTGTCGTGATGTTGCCAGAGTTACGCGCGCTTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                                   <  2:861576/150‑1 (MQ=255)
      aCCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATcac                                                                                                                                               <  1:671070/150‑1 (MQ=255)
        cGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACat                                                                                                                                             >  2:1566200/1‑150 (MQ=255)
          cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACatat                                                                                                                                           <  1:1065661/150‑1 (MQ=255)
          cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACatat                                                                                                                                           <  2:1181839/150‑1 (MQ=255)
          cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACatat                                                                                                                                           <  2:1015895/150‑1 (MQ=255)
           tATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATAtt                                                                                                                                          >  2:108495/1‑150 (MQ=255)
           tATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATAtt                                                                                                                                          <  2:1171755/150‑1 (MQ=255)
                   aGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATtgt                                                                                                                                  <  2:1082320/150‑1 (MQ=255)
                           tGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAAtt                                                                                                                          <  2:960860/150‑1 (MQ=255)
                               agagTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTAtt                                                                                                                      >  2:66023/1‑149 (MQ=255)
                               agagTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATg                                                                                                                      <  2:66617/150‑1 (MQ=255)
                                      cgcgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGGTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAaataa                                                                                                               >  1:39196/1‑150 (MQ=255)
                                        ctcgcgTAAAGTATGGTTTATAATTTAGTTTTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGGCTAAGGCTTCGTTTTATTGACGATAAATATTTGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATATTg                                                                                                             <  2:767761/148‑1 (MQ=255)
                                        cgggcttAAAGTAAGGTAAATAACTGTGTGTTTATTTTTGGTTTGTACGCGGTATGTTTTGGGGTTTTGCGGAAAAGGCGTCGTTTATTTTACTATAAATTTGTTATTCATATCAAATATTTATTTTGTTAATAATTTATTTAAATAATg                                                                                                             <  2:1121960/144‑1 (MQ=255)
                                        cgcgcgTAAAGTAATGTAAATAACTGAGTGGTTATTTTAGGGATCTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                             <  2:901638/150‑1 (MQ=255)
                                        cgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                             >  1:1391190/1‑150 (MQ=255)
                                                aaGTAAGGTAAATAACTTAGTGGTTATTTTAGGGATGTAATCTTTAAGTTTTGCGGTTGAGCGTCAAAGTCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                     <  1:1147975/150‑1 (MQ=255)
                                                aaGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                     <  2:1317105/150‑1 (MQ=255)
                                                aaGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                     <  1:978410/150‑1 (MQ=255)
                                                 aGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaa                                                                                                    >  2:587/1‑150 (MQ=255)
                                                  gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                                   >  2:1498293/1‑150 (MQ=255)
                                                   tAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAAt                                                                                                  <  1:1420636/150‑1 (MQ=255)
                                                     aGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTc                                                                                                <  1:677403/150‑1 (MQ=255)
                                                      ggTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCttttt                                                                                                      <  1:891294/143‑1 (MQ=255)
                                                      ggTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCttttt                                                                                                      >  2:891294/1‑143 (MQ=255)
                                                       gTAAATAACTGAGTGGTTATTTTATGGATGTAAGCGGTCAGTTTTGCGGTTGAGCATCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCag                                                                                              <  1:1354192/150‑1 (MQ=255)
                                                       gTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCag                                                                                              >  1:909708/1‑150 (MQ=255)
                                                         aaaTAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCagat                                                                                            >  1:670915/1‑149 (MQ=255)
                                                           aTAACTGAGTTGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGAAGATAAATATGTTATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtg                                                                                          <  1:66023/150‑1 (MQ=255)
                                                            tAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtgt                                                                                         >  1:373282/1‑150 (MQ=255)
                                                             aaCTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTCATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGtgtg                                                                                        <  1:1437298/150‑1 (MQ=255)
                                                              aCTGAGTGGTTATTTTAGGGATGTAAGCTGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGa                                                                                       <  1:493373/150‑1 (MQ=255)
                                                              aCTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGa                                                                                       >  2:1323520/1‑150 (MQ=255)
                                                               cTGAGTGGTTATTTTAGGGATGTAAGATGTCAGTTTTGCGGTTGAGCGGAAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGAATTTTTAAATTCAGAGTGTGaa                                                                                      >  2:1113960/1‑150 (MQ=255)
                                                               atGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGaa                                                                                      <  1:933891/149‑1 (MQ=255)
                                                                tGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGGTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                     <  2:39196/150‑1 (MQ=255)
                                                                  aGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATaa                                                                                   <  1:108495/150‑1 (MQ=255)
                                                                  aGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATaa                                                                                   >  2:1662658/1‑150 (MQ=255)
                                                                     ggTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAAt                                                                                <  1:1181214/150‑1 (MQ=255)
                                                                      gTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATAATGTCTTTTTAAATTCAGAGTGTGAATAAAAtt                                                                               >  1:258471/1‑150 (MQ=255)
                                                                       ttATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTc                                                                              <  1:1323520/150‑1 (MQ=255)
                                                                        tATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTTAGCGGCAAAGGCTTCGTTTAAGTGACGATAATTATGTGATTCATATGACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCTGTGTGTGAATAAAATTCa                                                                             <  2:511152/150‑1 (MQ=255)
                                                                         aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAATTAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCaa                                                                            >  1:111818/1‑149 (MQ=255)
                                                                         aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCAc                                                                            <  1:1404380/150‑1 (MQ=255)
                                                                            ttAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATTTTATTCAcact                                                                         <  2:191422/150‑4 (MQ=255)
                                                                             tAGGGATGTAAGCGGTCAGTTTTTCGGTTGAGCGGCAAAGGGGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGGTTTTTAAATTCAGAGTGTGAATTTTATTCAcactc                                                                        <  2:96352/150‑5 (MQ=255)
                                                                             tAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATCGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCgg                                                                        <  1:1426397/150‑1 (MQ=255)
                                                                               tggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGTCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                      <  2:803409/149‑1 (MQ=255)
                                                                               gggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                      >  2:687947/1‑150 (MQ=255)
                                                                                   tGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAAt                                                                  >  2:1110510/1‑150 (MQ=255)
                                                                                          ggTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCcgcg                                                           <  1:456231/150‑1 (MQ=255)
                                                                                            tCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCggg                                                         <  2:149291/150‑1 (MQ=255)
                                                                                             cAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGTGTAATCTCCGCGGGa                                                        >  2:765078/1‑150 (MQ=255)
                                                                                             cAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGa                                                        >  2:1216311/1‑150 (MQ=255)
                                                                                              aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                     >  2:756519/1‑120 (MQ=255)
                                                                                              aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                     <  1:756519/120‑1 (MQ=255)
                                                                                              aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGat                                                       >  1:1620869/1‑150 (MQ=255)
                                                                                               gTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGata                                                      >  2:28354/1‑150 (MQ=255)
                                                                                                      ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATTGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                               >  2:964399/1‑150 (MQ=255)
                                                                                                      ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                               <  1:1498293/150‑1 (MQ=255)
                                                                                                         tGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGt                                            >  1:197981/1‑150 (MQ=255)
                                                                                                                    ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                 >  1:264205/1‑150 (MQ=255)
                                                                                                                    ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                 >  1:322274/1‑150 (MQ=255)
                                                                                                                    ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                 >  1:906493/1‑150 (MQ=255)
                                                                                                                      cgtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAAGAATGGCTTTTTAAATTCAGAGTGTGATTAAAATTCACTCGGCTTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCAAg                               >  1:309747/1‑150 (MQ=255)
                                                                                                                       gtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAgg                              <  2:1620869/150‑1 (MQ=255)
                                                                                                                        tcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGc                             <  1:1144133/150‑1 (MQ=255)
                                                                                                                        tcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGATTTTTAATTTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCATTTCAAGGc                             >  2:1578192/1‑150 (MQ=255)
                                                                                                                         cgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGcc                            <  1:73732/150‑1 (MQ=255)
                                                                                                                          gtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCg                           >  2:1416017/1‑150 (MQ=255)
                                                                                                                                gTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCTAAAATc                     >  1:1491457/1‑150 (MQ=255)
                                                                                                                                  gACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCtt                   >  1:679210/1‑150 (MQ=255)
                                                                                                                                   aataTAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATCTAAATAATGGCTTTTTAAATTCATAGTGTTAATAAAATTCACTCGGAGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCAAGGCCGAAAATCTTg                  >  1:1133239/4‑150 (MQ=255)
                                                                                                                                   aCGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  <  1:103080/150‑1 (MQ=255)
                                                                                                                                   aCGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  <  2:373282/150‑1 (MQ=255)
                                                                                                                                   aCGATAAATATGGGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  <  1:1487437/150‑1 (MQ=255)
                                                                                                                                    cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc                 >  2:912477/1‑150 (MQ=255)
                                                                                                                                    agATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc                 <  1:181198/149‑1 (MQ=255)
                                                                                                                                     gATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCa                >  1:624814/1‑150 (MQ=255)
                                                                                                                                      aTAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  <  2:1137615/147‑1 (MQ=255)
                                                                                                                                      aTAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                  >  1:1137615/1‑147 (MQ=255)
                                                                                                                                       tAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATc              >  1:382011/1‑150 (MQ=255)
                                                                                                                                         aaTATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCgg            <  2:1144073/150‑1 (MQ=255)
                                                                                                                                         aaTATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTAATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCgg            >  2:320407/1‑150 (MQ=255)
                                                                                                                                         aaTATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTAAATCGGCTTAATCTCAGCTGTATATTCATAAAGTTTTTCCTTTCAATGCCGAAAATCTTGAATCgg            >  1:248640/1‑150 (MQ=255)
                                                                                                                                          atatGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTAAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGAGGGATATTCATAAAGTTTTTACTTTCAAGGCCGAAAATCTTGCATCgga           >  2:280932/1‑149 (MQ=255)
                                                                                                                                           tatGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTc          >  2:1323904/1‑150 (MQ=255)
                                                                                                                                             tgtgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTAACTCGGCTTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCca        >  1:1112984/1‑150 (MQ=255)
                                                                                                                                               tgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCcaca      <  1:1666716/150‑1 (MQ=255)
                                                                                                                                               tgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCcaca      <  2:909708/150‑1 (MQ=255)
                                                                                                                                                gATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg     <  2:322274/150‑1 (MQ=255)
                                                                                                                                                gATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg     >  1:850833/1‑150 (MQ=255)
                                                                                                                                                  ttCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGa   <  1:28354/150‑1 (MQ=255)
                                                                                                                                                   tCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGaa  <  2:670915/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAA  >  NC_000913/4591351‑4591647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: