Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,591,500 A→G 7.4% intergenic (‑221/‑157) yjiY ← / → tsr putative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,591,5000AG 7.4% 349.4 / 14.1 122intergenic (‑221/‑157)yjiY/tsrputative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor
Reads supporting (aligned to +/- strand):  ref base A (56/57);  new base G (6/3);  total (62/60)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 130 total aligned reads displayed.

AAATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAG  >  NC_000913/4591351‑4591649
                                                                                                                                                     |                                                                                                                                                     
aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                       >  2:1213544/1‑150 (MQ=255)
aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                       <  2:523768/150‑1 (MQ=255)
aaaTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCa                                                                                                                                                       <  2:530557/150‑1 (MQ=255)
  aTTGACCGCTATCCTGTAGTGATGGTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCTTCGTTTAAGTGACGATAAATATGTGATACata                                                                                                                                                     >  1:136928/1‑150 (MQ=255)
  aTTGACCGCCATCCTGTCGTGATGTTGCCAGAGTTACGCGCGCTTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                                     <  2:861576/150‑1 (MQ=255)
      aCCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATcac                                                                                                                                                 <  1:665061/150‑1 (MQ=255)
          cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACatat                                                                                                                                             <  1:1065661/150‑1 (MQ=255)
          cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACatat                                                                                                                                             <  2:1181839/150‑1 (MQ=255)
          cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACatat                                                                                                                                             <  2:1015895/150‑1 (MQ=255)
               cTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATATTtatc                                                                                                                                        >  1:377845/1‑149 (MQ=255)
                   aGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATtgt                                                                                                                                    <  2:1082320/150‑1 (MQ=255)
                      gATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGaa                                                                                                                                 >  2:1666716/1‑150 (MQ=255)
                        tGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCATAGGTGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTTTATTGTGaata                                                                                                                               >  2:1144133/1‑150 (MQ=255)
                           tGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAAtt                                                                                                                            <  2:960860/150‑1 (MQ=255)
                               agagTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTAtt                                                                                                                        >  2:66023/1‑149 (MQ=255)
                               agagTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATg                                                                                                                        <  2:66617/150‑1 (MQ=255)
                                        ctcgcgTAAAGTATGGTTTATAATTTAGTTTTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGGCTAAGGCTTCGTTTTATTGACGATAAATATTTGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATATTg                                                                                                               <  2:767761/148‑1 (MQ=255)
                                        cgcgcgTAAAGTAATGTAAATAACTGAGTGGTTATTTTAGGGATCTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                               <  2:901638/150‑1 (MQ=255)
                                        cgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                               >  1:1391190/1‑150 (MQ=255)
                                                aaGTAAGGTAAATAACTTAGTGGTTATTTTAGGGATGTAATCTTTAAGTTTTGCGGTTGAGCGTCAAAGTCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                       <  1:1147975/150‑1 (MQ=255)
                                                aaGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                       <  1:978410/150‑1 (MQ=255)
                                                  gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                                     >  1:14437/1‑150 (MQ=255)
                                                  gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                                     >  2:1498293/1‑150 (MQ=255)
                                                   tAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAAt                                                                                                    <  1:1420636/150‑1 (MQ=255)
                                                     aGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTc                                                                                                  <  1:677403/150‑1 (MQ=255)
                                                      ggTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCttttt                                                                                                        >  2:891294/1‑143 (MQ=255)
                                                       gTAAATAACTGAGTGGTTATTTTATGGATGTAAGCGGTCAGTTTTGCGGTTGAGCATCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCag                                                                                                <  1:1354192/150‑1 (MQ=255)
                                                       gTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCag                                                                                                >  1:909708/1‑150 (MQ=255)
                                                         aaaTAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCagat                                                                                              >  1:670915/1‑149 (MQ=255)
                                                           aTAACTGAGTTGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGAAGATAAATATGTTATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtg                                                                                            <  1:66023/150‑1 (MQ=255)
                                                            tAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtgt                                                                                           >  2:103080/1‑150 (MQ=255)
                                                            tAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtgt                                                                                           >  1:373282/1‑150 (MQ=255)
                                                              aCTGAGTGGTTATTTTAGGGATGTAAGCTGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGa                                                                                         <  1:493373/150‑1 (MQ=255)
                                                              aCTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGa                                                                                         >  2:1323520/1‑150 (MQ=255)
                                                               cTGAGTGGTTATTTTAGGGATGTAAGATGTCAGTTTTGCGGTTGAGCGGAAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGAATTTTTAAATTCAGAGTGTGaa                                                                                        >  2:1113960/1‑150 (MQ=255)
                                                                tGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGGTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                       <  2:39196/150‑1 (MQ=255)
                                                                  aGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATaa                                                                                     <  1:108495/150‑1 (MQ=255)
                                                                  aGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATaa                                                                                     >  2:1662658/1‑150 (MQ=255)
                                                                     ggTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAAt                                                                                  <  1:1181214/150‑1 (MQ=255)
                                                                        tATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTTAGCGGCAAAGGCTTCGTTTAAGTGACGATAATTATGTGATTCATATGACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCTGTGTGTGAATAAAATTCa                                                                               <  2:511152/150‑1 (MQ=255)
                                                                         aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAATTAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCaa                                                                              >  1:111818/1‑149 (MQ=255)
                                                                         aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCAc                                                                              <  1:1404380/150‑1 (MQ=255)
                                                                             tAGGGATGTAAGCGGTCAGTTTTTCGGTTGAGCGGCAAAGGGGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGGTTTTTAAATTCAGAGTGTGAATTTTATTCAcactc                                                                          <  2:96352/150‑5 (MQ=255)
                                                                             tAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATCGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCgg                                                                          <  1:1426397/150‑1 (MQ=255)
                                                                               tggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGTCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                        <  2:803409/149‑1 (MQ=255)
                                                                               gggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                        >  2:687947/1‑150 (MQ=255)
                                                                                ggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATAACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGAGAATAAAATTCACTCGGCGt                                                                       >  1:201767/1‑150 (MQ=255)
                                                                                   tGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAAt                                                                    >  2:1110510/1‑150 (MQ=255)
                                                                                    gTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAAtc                                                                   <  1:48337/150‑1 (MQ=255)
                                                                                          ggTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCcgcg                                                             <  1:456231/150‑1 (MQ=255)
                                                                                           gTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCgg                                                            >  1:794707/1‑150 (MQ=255)
                                                                                           gTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTAGGCGTAAtctcagagg                                                            >  1:158352/1‑145 (MQ=255)
                                                                                            tCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCggg                                                           <  2:149291/150‑1 (MQ=255)
                                                                                             cAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGTGTAATCTCCGCGGGa                                                          >  2:765078/1‑150 (MQ=255)
                                                                                             cAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGa                                                          >  2:1216311/1‑150 (MQ=255)
                                                                                              aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                       <  1:756519/120‑1 (MQ=255)
                                                                                              aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                       >  2:756519/1‑120 (MQ=255)
                                                                                              aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGat                                                         >  1:1620869/1‑150 (MQ=255)
                                                                                               gTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGata                                                        >  2:28354/1‑150 (MQ=255)
                                                                                                      ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATTGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                                 >  2:964399/1‑150 (MQ=255)
                                                                                                      ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                                 <  1:1498293/150‑1 (MQ=255)
                                                                                                         tGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGt                                              >  1:197981/1‑150 (MQ=255)
                                                                                                               gCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcc                                        <  1:687947/150‑1 (MQ=255)
                                                                                                                    ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                   >  1:264205/1‑150 (MQ=255)
                                                                                                                    ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                   >  1:322274/1‑150 (MQ=255)
                                                                                                                    ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                   >  1:906493/1‑150 (MQ=255)
                                                                                                                       gtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAgg                                <  2:1620869/150‑1 (MQ=255)
                                                                                                                       gtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATATCCGCGGGATATTCATAAAGTTTTTACTTTCCAgg                                >  1:27587/1‑150 (MQ=255)
                                                                                                                        tcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGc                               <  1:1144133/150‑1 (MQ=255)
                                                                                                                        tcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGATTTTTAATTTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCATTTCAAGGc                               >  2:1578192/1‑150 (MQ=255)
                                                                                                                         cgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGcc                              <  1:73732/150‑1 (MQ=255)
                                                                                                                                 acaataTAAATATGTGATATGAATCGCATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCt                      >  2:155429/6‑150 (MQ=255)
                                                                                                                                  gACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCtt                     >  1:922984/1‑150 (MQ=255)
                                                                                                                                  gACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCtt                     >  1:679210/1‑150 (MQ=255)
                                                                                                                                   aataTAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATCTAAATAATGGCTTTTTAAATTCATAGTGTTAATAAAATTCACTCGGAGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCAAGGCCGAAAATCTTg                    >  1:1133239/4‑150 (MQ=255)
                                                                                                                                   aCGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    <  2:373282/150‑1 (MQ=255)
                                                                                                                                   aCGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    <  1:103080/150‑1 (MQ=255)
                                                                                                                                   aCGATAAATATGGGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    <  1:1487437/150‑1 (MQ=255)
                                                                                                                                    cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc                   >  2:912477/1‑150 (MQ=255)
                                                                                                                                    cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTATCCTTTCCAGGCCGAAAATCTTGc                   >  2:1217242/1‑150 (MQ=255)
                                                                                                                                      aTAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    <  2:1137615/147‑1 (MQ=255)
                                                                                                                                      aTAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    >  1:1137615/1‑147 (MQ=255)
                                                                                                                                       tAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATc                <  2:136928/150‑1 (MQ=255)
                                                                                                                                       tAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATc                >  1:382011/1‑150 (MQ=255)
                                                                                                                                        aaaTATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGCAAATCTTGCATCg               >  2:305670/1‑150 (MQ=255)
                                                                                                                                         aaTATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCgg              <  2:1144073/150‑1 (MQ=255)
                                                                                                                                         aaTATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTAATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCgg              >  2:320407/1‑150 (MQ=255)
                                                                                                                                         aaTATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTAAATCGGCTTAATCTCAGCTGTATATTCATAAAGTTTTTCCTTTCAATGCCGAAAATCTTGAATCgg              >  1:248640/1‑150 (MQ=255)
                                                                                                                                          atatGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTAAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGAGGGATATTCATAAAGTTTTTACTTTCAAGGCCGAAAATCTTGCATCgga             >  2:280932/1‑149 (MQ=255)
                                                                                                                                           tatGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTc            >  2:1323904/1‑150 (MQ=255)
                                                                                                                                             tgtgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTAACTCGGCTTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCca          >  1:1112984/1‑150 (MQ=255)
                                                                                                                                               tgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCcaca        <  2:909708/150‑1 (MQ=255)
                                                                                                                                               tgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCcaca        <  1:1666716/150‑1 (MQ=255)
                                                                                                                                                gATTCATATCACATTTTTATATTGTTAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGGGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg       <  2:111818/150‑1 (MQ=255)
                                                                                                                                                gATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg       >  1:850833/1‑150 (MQ=255)
                                                                                                                                                gATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg       <  2:322274/150‑1 (MQ=255)
                                                                                                                                                  ttCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGa     <  1:28354/150‑1 (MQ=255)
                                                                                                                                                   tCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTGAGATTGTGATTAAAATTCACTCGGCGTAATATCCGGGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCTTCGGTCCACAGGaa    <  1:1323960/150‑1 (MQ=255)
                                                                                                                                                   tCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGaa    <  2:670915/150‑1 (MQ=255)
                                                                                                                                                     atatCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAag  >  1:1445379/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAG  >  NC_000913/4591351‑4591649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: