Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,591,501 T→A 7.1% intergenic (‑222/‑156) yjiY ← / → tsr putative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,591,5010TA 7.1% 323.5 / 10.2 122intergenic (‑222/‑156)yjiY/tsrputative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor
Reads supporting (aligned to +/- strand):  ref base T (56/57);  new base A (6/3);  total (62/60)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 126 total aligned reads displayed.

ATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAG  >  NC_000913/4591353‑4591649
                                                                                                                                                    |                                                                                                                                                    
aTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGCGCGGCAAAGGCTTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                                     >  2:316024/1‑150 (MQ=255)
aTTGACCGCTATCCTGTAGTGATGGTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCTTCGTTTAAGTGACGATAAATATGTGATACata                                                                                                                                                     >  1:136928/1‑150 (MQ=255)
aTTGACCGCCATCCTGTCGTGATGTTGCCAGAGTTACGCGCGCTTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                                     <  2:861576/150‑1 (MQ=255)
    aCCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATcac                                                                                                                                                 <  1:665061/150‑1 (MQ=255)
    aCCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATcac                                                                                                                                                 <  1:671070/150‑1 (MQ=255)
      cGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACat                                                                                                                                               >  2:1566200/1‑150 (MQ=255)
        cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACatat                                                                                                                                             <  1:1065661/150‑1 (MQ=255)
        cTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACatat                                                                                                                                             <  2:1015895/150‑1 (MQ=255)
         tATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATAtt                                                                                                                                            >  2:108495/1‑150 (MQ=255)
         tATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATAtt                                                                                                                                            <  2:1171755/150‑1 (MQ=255)
             cTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATATTtatc                                                                                                                                        >  1:377845/1‑149 (MQ=255)
                      tGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCATAGGTGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTTTATTGTGaata                                                                                                                               >  2:1144133/1‑150 (MQ=255)
                         tGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAAtt                                                                                                                            <  2:960860/150‑1 (MQ=255)
                             agagTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTAtt                                                                                                                        >  2:66023/1‑149 (MQ=255)
                                      ctcgcgTAAAGTATGGTTTATAATTTAGTTTTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGGCTAAGGCTTCGTTTTATTGACGATAAATATTTGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATATTg                                                                                                               <  2:767761/148‑1 (MQ=255)
                                      cgcgcgTAAAGTAATGTAAATAACTGAGTGGTTATTTTAGGGATCTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                               <  2:901638/150‑1 (MQ=255)
                                      cgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                               >  1:1391190/1‑150 (MQ=255)
                                              aaGTAAGGTAAATAACTTAGTGGTTATTTTAGGGATGTAATCTTTAAGTTTTGCGGTTGAGCGTCAAAGTCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                       <  1:1147975/150‑1 (MQ=255)
                                              aaGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                       <  1:978410/150‑1 (MQ=255)
                                              aaGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTa                                                                                                       <  2:1317105/150‑1 (MQ=255)
                                               aGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaa                                                                                                      >  2:587/1‑150 (MQ=255)
                                               aGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaa                                                                                                      >  2:679866/1‑150 (MQ=255)
                                                gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                                     >  1:14437/1‑150 (MQ=255)
                                                gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                                     >  2:1498293/1‑150 (MQ=255)
                                                   aGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAAtta                                                                                                  >  2:897844/1‑149 (MQ=255)
                                                   aGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTc                                                                                                  <  1:677403/150‑1 (MQ=255)
                                                    ggTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCttttt                                                                                                        <  1:891294/143‑1 (MQ=255)
                                                     gTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCag                                                                                                >  1:909708/1‑150 (MQ=255)
                                                       aaaTAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCagat                                                                                              >  1:670915/1‑149 (MQ=255)
                                                          tAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtgt                                                                                           >  2:103080/1‑150 (MQ=255)
                                                          tAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtgt                                                                                           >  1:373282/1‑150 (MQ=255)
                                                           aaCTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTCATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGtgtg                                                                                          <  1:1437298/150‑1 (MQ=255)
                                                            aCTGAGTTGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGGCAAAGGCGTCGTTTAAGTGAAGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGa                                                                                         <  2:767745/150‑1 (MQ=255)
                                                            aCTGAGTGGTTATTTTAGGGATGTAAGCTGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGa                                                                                         <  1:493373/150‑1 (MQ=255)
                                                            aCTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGa                                                                                         >  2:1323520/1‑150 (MQ=255)
                                                             cTGAGTGGTTATTTTAGGGATGTAAGATGTCAGTTTTGCGGTTGAGCGGAAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGAATTTTTAAATTCAGAGTGTGaa                                                                                        >  2:1113960/1‑150 (MQ=255)
                                                             atGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGaa                                                                                        <  1:933891/149‑1 (MQ=255)
                                                              tGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGGTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                       <  2:39196/150‑1 (MQ=255)
                                                                aGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGTCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATaa                                                                                     <  1:108495/150‑1 (MQ=255)
                                                                aGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATaa                                                                                     >  2:1662658/1‑150 (MQ=255)
                                                                   ggTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAAt                                                                                  <  1:1181214/150‑1 (MQ=255)
                                                                    gTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATTATTTATGTAAATAATGTCTTTTTAAATTCAGAGTGTGAATAAAAtt                                                                                 >  1:258471/1‑150 (MQ=255)
                                                                     ttATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTc                                                                                <  1:1323520/150‑1 (MQ=255)
                                                                      tATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTTAGCGGCAAAGGCTTCGTTTAAGTGACGATAATTATGTGATTCATATGACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCTGTGTGTGAATAAAATTCa                                                                               <  2:511152/150‑1 (MQ=255)
                                                                       aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAATTAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCaa                                                                              >  1:111818/1‑149 (MQ=255)
                                                                       aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCAc                                                                              <  1:1404380/150‑1 (MQ=255)
                                                                          ttAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATTTTATTCAcact                                                                           <  2:191422/150‑4 (MQ=255)
                                                                           tAGGGATGTAAGCGGTCAGTTTTTCGGTTGAGCGGCAAAGGGGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGGTTTTTAAATTCAGAGTGTGAATTTTATTCAcactc                                                                          <  2:96352/150‑5 (MQ=255)
                                                                           tAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATCGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCgg                                                                          <  1:1426397/150‑1 (MQ=255)
                                                                             tggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGTCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                        <  2:803409/149‑1 (MQ=255)
                                                                             gggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                        >  2:687947/1‑150 (MQ=255)
                                                                              ggATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATAACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGAGAATAAAATTCACTCGGCGt                                                                       >  1:201767/1‑150 (MQ=255)
                                                                                 tGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAAt                                                                    >  2:1110510/1‑150 (MQ=255)
                                                                                  gTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAAtc                                                                   <  1:48337/150‑1 (MQ=255)
                                                                                        ggTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCcgcg                                                             <  1:456231/150‑1 (MQ=255)
                                                                                         gTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCgg                                                            >  1:794707/1‑150 (MQ=255)
                                                                                         gTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTAGGCGTAAtctcagagg                                                            >  1:158352/1‑145 (MQ=255)
                                                                                          tCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCggg                                                           <  2:149291/150‑1 (MQ=255)
                                                                                           cAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGa                                                          >  2:1216311/1‑150 (MQ=255)
                                                                                            aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                       <  1:756519/120‑1 (MQ=255)
                                                                                            aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAAt                                                                                       >  2:756519/1‑120 (MQ=255)
                                                                                            aGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGat                                                         >  1:1620869/1‑150 (MQ=255)
                                                                                             gTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGata                                                        >  2:28354/1‑150 (MQ=255)
                                                                                                    ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATTGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                                 >  2:964399/1‑150 (MQ=255)
                                                                                                    ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                                 <  1:1498293/150‑1 (MQ=255)
                                                                                                       tGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGt                                              >  1:197981/1‑150 (MQ=255)
                                                                                                          gCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGtttt                                           >  1:1144073/1‑150 (MQ=255)
                                                                                                             gCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcc                                        <  1:687947/150‑1 (MQ=255)
                                                                                                                  ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                   >  1:264205/1‑150 (MQ=255)
                                                                                                                  ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                   >  1:906493/1‑150 (MQ=255)
                                                                                                                  ggCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcctttcc                                   >  1:322274/1‑150 (MQ=255)
                                                                                                                     gtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAgg                                <  2:1620869/150‑1 (MQ=255)
                                                                                                                     gtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATATCCGCGGGATATTCATAAAGTTTTTACTTTCCAgg                                >  1:27587/1‑150 (MQ=255)
                                                                                                                      tcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGATTTTTAATTTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCATTTCAAGGc                               >  2:1578192/1‑150 (MQ=255)
                                                                                                                       cgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGcc                              <  1:73732/150‑1 (MQ=255)
                                                                                                                        gtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCg                             >  2:1416017/1‑150 (MQ=255)
                                                                                                                            aaGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGaaaa                         >  1:1403706/1‑150 (MQ=255)
                                                                                                                              gTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCTAAAATc                       >  1:1491457/1‑150 (MQ=255)
                                                                                                                                 aataTAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATCTAAATAATGGCTTTTTAAATTCATAGTGTTAATAAAATTCACTCGGAGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCAAGGCCGAAAATCTTg                    >  1:1133239/4‑150 (MQ=255)
                                                                                                                                 aCGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    <  2:373282/150‑1 (MQ=255)
                                                                                                                                 aCGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    <  1:103080/150‑1 (MQ=255)
                                                                                                                                  cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc                   >  2:912477/1‑150 (MQ=255)
                                                                                                                                  cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTATCCTTTCCAGGCCGAAAATCTTGc                   >  2:1217242/1‑150 (MQ=255)
                                                                                                                                  agATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc                   <  1:181198/149‑1 (MQ=255)
                                                                                                                                   gATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCa                  >  1:624814/1‑150 (MQ=255)
                                                                                                                                    aTAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    >  1:1137615/1‑147 (MQ=255)
                                                                                                                                    aTAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTg                    <  2:1137615/147‑1 (MQ=255)
                                                                                                                                     tAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATc                <  2:136928/150‑1 (MQ=255)
                                                                                                                                     tAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATc                >  1:382011/1‑150 (MQ=255)
                                                                                                                                       aaTATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCgg              <  2:1144073/150‑1 (MQ=255)
                                                                                                                                       aaTATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTAATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCgg              >  2:320407/1‑150 (MQ=255)
                                                                                                                                        atatGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTAAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGAGGGATATTCATAAAGTTTTTACTTTCAAGGCCGAAAATCTTGCATCgga             >  2:280932/1‑149 (MQ=255)
                                                                                                                                           tgtgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTAACTCGGCTTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCca          >  1:1112984/1‑150 (MQ=255)
                                                                                                                                             tgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCcaca        <  2:909708/150‑1 (MQ=255)
                                                                                                                                              gATTCATATCACATTTTTATATTGTTAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGGGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg       <  2:111818/150‑1 (MQ=255)
                                                                                                                                              gATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAg       <  2:322274/150‑1 (MQ=255)
                                                                                                                                                ttCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGa     <  1:28354/150‑1 (MQ=255)
                                                                                                                                                 tCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTGAGATTGTGATTAAAATTCACTCGGCGTAATATCCGGGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCTTCGGTCCACAGGaa    <  1:1323960/150‑1 (MQ=255)
                                                                                                                                                 tCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGaa    <  2:670915/150‑1 (MQ=255)
                                                                                                                                                   atatCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAag  >  1:1445379/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAG  >  NC_000913/4591353‑4591649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: