Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,593,431 A→G 7.4% V431A (GTC→GCC) ‡ lgoT ← putative L‑galactonate:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,4310AG 7.4% 297.0 / 12.7 108V431A (GTC→GCC) ‡lgoTputative L‑galactonate:H+ symporter
Reads supporting (aligned to +/- strand):  ref base A (44/56);  new base G (2/6);  total (46/62)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

Only 100 of 108 total aligned reads displayed.

GATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGA  >  NC_000913/4593283‑4593578
                                                                                                                                                    |                                                                                                                                                   
gATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGAc                                                                                                                                                    <  1:1228718/150‑1 (MQ=255)
 agaGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACg                                                                                                                                                   <  1:1256860/148‑1 (MQ=255)
     cGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAAc                                                                                                                                               <  1:1309522/150‑1 (MQ=255)
       aggagAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCTTAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCACCGCTGCGGTGACGCAACCg                                                                                                                                             >  1:276809/1‑150 (MQ=255)
         gagaACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCa                                                                                                                                           <  1:1534292/150‑1 (MQ=255)
           gaACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgc                                                                                                                                         <  1:371092/150‑2 (MQ=255)
             aCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgcgg                                                                                                                                       <  1:657784/150‑4 (MQ=255)
               tGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgatgat                                                                                                                                     >  2:133145/1‑150 (MQ=255)
                gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgcggggg                                                                                                                                    <  2:504640/150‑7 (MQ=255)
                 ggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTa                                                                                                                                   >  1:1056793/1‑150 (MQ=255)
                 ggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTa                                                                                                                                   >  1:1675392/1‑150 (MQ=255)
                    aaCATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTg                                                                                                                                <  1:343460/150‑1 (MQ=255)
                      cATTTTAATCGCCATTAAAATGCCCGATAAGAAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGcc                                                                                                                              <  2:276809/150‑1 (MQ=255)
                      cATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGcc                                                                                                                              >  2:283208/1‑150 (MQ=255)
                        ttttAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAg                                                                                                                            <  2:1023896/150‑1 (MQ=255)
                        ttttAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAg                                                                                                                            >  2:410827/1‑150 (MQ=255)
                            aaTCTCGATGATAAAGCCCGATAAGCAAAATGATATCGGGCATAAGGAGAATAATCTTTACGTGCGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACgg                                                                                                                        <  1:253368/150‑1 (MQ=255)
                                gCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATg                                                                                                                    <  2:876854/150‑1 (MQ=255)
                                      aaaaTGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAgtggg                                                                                                              <  2:417256/150‑1 (MQ=255)
                                        aaTGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAgtgggtg                                                                                                            >  2:1429253/1‑150 (MQ=255)
                                         aTGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAgtgggtgg                                                                                                           <  1:1712212/150‑1 (MQ=255)
                                            cccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACTGAATGAGTGGGTGGTAt                                                                                                        >  1:1385349/1‑150 (MQ=255)
                                            cccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTAt                                                                                                        <  2:958322/150‑1 (MQ=255)
                                            cccGATAAGCAAAATCTTAACGGGCATAAGGAGATTAATCTATAAGTGGCTCGTTTATCGGCTCACGAACCAGGAAGATGTACGCCAGTGCCCACGCTGCGGTCACGCAACCGCAGAAGATTAGTGCCAGACGGAATGAGTGGGTGCTAt                                                                                                        >  1:872270/1‑150 (MQ=255)
                                             ccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATc                                                                                                       <  1:730828/150‑1 (MQ=255)
                                             ccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATc                                                                                                       <  2:416461/150‑1 (MQ=255)
                                                aTAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaac                                                                                                    <  2:1131795/150‑1 (MQ=255)
                                                 tAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaaca                                                                                                   <  1:783703/150‑1 (MQ=255)
                                                  aaGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaacaa                                                                                                  <  2:185578/150‑1 (MQ=255)
                                                   aGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAAt                                                                                                 <  1:1657686/150‑1 (MQ=255)
                                                   aGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAAt                                                                                                 >  1:935518/1‑150 (MQ=255)
                                                     cAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGGCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATaa                                                                                               <  2:885161/150‑1 (MQ=255)
                                                      aaaaTGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGGTGCTCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTACTGCCAGACGGAAGGAGTGGGTCGGAGCAACAAGaaa                                                                                              >  1:47655/1‑150 (MQ=255)
                                                        aaTGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAAc                                                                                            >  1:1627622/1‑150 (MQ=255)
                                                           tttATCGGGCATAAGGAGATTAATCTTTACGTGGGGCGGTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGGCGCAAACGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAg                                                                                         <  2:851387/149‑1 (MQ=255)
                                                            ttttCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGt                                                                                        <  2:1276844/147‑1 (MQ=255)
                                                             tATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTa                                                                                       >  1:1244980/1‑150 (MQ=255)
                                                              aTCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTaa                                                                                      >  1:1534046/1‑150 (MQ=255)
                                                                  ggCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGAt                                                                                  >  2:713032/1‑150 (MQ=255)
                                                                   gCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATc                                                                                 <  2:1675392/150‑1 (MQ=255)
                                                                   gCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATc                                                                                 <  1:1600891/150‑1 (MQ=255)
                                                                    cATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCg                                                                                <  2:430079/150‑1 (MQ=255)
                                                                    cATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCg                                                                                <  2:407923/150‑1 (MQ=255)
                                                                      tAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgc                                                                              <  2:1488495/150‑1 (MQ=255)
                                                                      tAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgc                                                                              <  2:1221329/150‑1 (MQ=255)
                                                                      tAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgc                                                                              >  2:1008897/1‑150 (MQ=255)
                                                                       aaGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcg                                                                             <  2:1457524/150‑1 (MQ=255)
                                                                        aGGAGATTATGCTTTACGTGGGTCGTTGATCGGCTGACGACCCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcgc                                                                            <  2:47655/150‑1 (MQ=255)
                                                                         ggAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTTGTATCAACAATAAAACCAGTAATGATCGGCGCa                                                                           <  1:1240307/150‑1 (MQ=255)
                                                                              ttAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAaga                                                                      >  2:1141912/1‑150 (MQ=255)
                                                                                aaTCTTTACGTGGGGCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAgg                                                                    <  2:1341402/150‑1 (MQ=255)
                                                                                   cTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGgcgc                                                                 >  2:1536963/1‑150 (MQ=255)
                                                                                     ttACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAg                                                               >  1:285823/1‑150 (MQ=255)
                                                                                        cGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATg                                                            >  2:638784/1‑150 (MQ=255)
                                                                                         gtgtgtCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCGCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGa                                                           <  2:266387/150‑1 (MQ=255)
                                                                                          tGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATgga                                                          >  2:581394/1‑148 (MQ=255)
                                                                                            ggTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGc                                                        <  1:1141912/150‑1 (MQ=255)
                                                                                              tCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTg                                                      <  1:133145/150‑1 (MQ=255)
                                                                                              tCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTg                                                      <  2:1432265/150‑1 (MQ=255)
                                                                                               cGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTgg                                                     <  2:1627622/150‑1 (MQ=255)
                                                                                                ggtGATCTGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGGGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGc                                                    <  2:1607631/148‑1 (MQ=255)
                                                                                                gTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGc                                                    <  1:646184/150‑1 (MQ=255)
                                                                                                gTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGc                                                    <  2:581886/150‑1 (MQ=255)
                                                                                                  tGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATAAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCaa                                                  >  1:1612147/1‑150 (MQ=255)
                                                                                                   gATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCaaa                                                 >  1:1709790/1‑150 (MQ=255)
                                                                                                      cGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGtt                                              >  2:823136/1‑150 (MQ=255)
                                                                                                       ggCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGtta                                             >  1:617476/1‑149 (MQ=255)
                                                                                                         cTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAGGAGGCGCAGATGAAGCTGGCAAAGTTCTg                                           <  1:492558/150‑1 (MQ=255)
                                                                                                         cTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTg                                           <  1:601119/150‑1 (MQ=255)
                                                                                                          tGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTgg                                          <  2:1244980/150‑1 (MQ=255)
                                                                                                           gACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGa                                         <  1:1252889/150‑1 (MQ=255)
                                                                                                             cGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATa                                       >  1:1630602/1‑150 (MQ=255)
                                                                                                               aaCCAGGTAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACt                                     <  1:1273174/150‑1 (MQ=255)
                                                                                                               aaCCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACt                                     <  2:935518/150‑1 (MQ=255)
                                                                                                                aCCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTg                                    >  2:1701346/1‑150 (MQ=255)
                                                                                                                    ggAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCa                                >  2:1522045/1‑150 (MQ=255)
                                                                                                                      aaGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGTATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCAcc                              >  1:487311/1‑150 (MQ=255)
                                                                                                                      aaGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCAcc                              >  1:816249/1‑150 (MQ=255)
                                                                                                                       aGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGGGGGATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCAccc                             >  1:110691/1‑149 (MQ=255)
                                                                                                                         aTGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGAc                           <  1:638784/150‑1 (MQ=255)
                                                                                                                         aTGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGAc                           <  2:1056793/150‑1 (MQ=255)
                                                                                                                          tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCTCCGACg                          >  1:315803/1‑150 (MQ=255)
                                                                                                                          tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACg                          <  1:713032/150‑1 (MQ=255)
                                                                                                                          tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACg                          <  1:1522045/150‑1 (MQ=255)
                                                                                                                            tACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCa                        >  2:1665969/1‑150 (MQ=255)
                                                                                                                                ccAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCa                    <  1:410827/150‑1 (MQ=255)
                                                                                                                                  aGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATg                  >  2:39313/1‑150 (MQ=255)
                                                                                                                                  aGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATg                  >  2:1179016/1‑150 (MQ=255)
                                                                                                                                   gTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGc                 >  1:530358/1‑150 (MQ=255)
                                                                                                                                      cccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGTCATGCgag              >  1:456204/1‑150 (MQ=255)
                                                                                                                                      aatcatcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGTCATGCgag              >  1:381943/7‑150 (MQ=255)
                                                                                                                                          cGCTTCGGTGACGCAACCTCAGATGATTAGGGCCAGACGGAATGAGTGGGTGGTATCAAAAATATAACCTGTTATGATCGGCGCATAAGAGGCGCAGAGGAAGCTGGCAATGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAgg          <  2:872270/150‑2 (MQ=255)
                                                                                                                                          cGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATATTGCCCACCGACGCAGTCATGCGCGAAGc          >  2:320943/1‑150 (MQ=255)
                                                                                                                                          cGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGc          <  2:1330840/150‑1 (MQ=255)
                                                                                                                                          cGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGc          <  2:392466/150‑1 (MQ=255)
                                                                                                                                           tcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCt                                                       <  2:86675/103‑1 (MQ=255)
                                                                                                                                           tcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCt                                                       >  1:86675/2‑104 (MQ=255)
                                                                                                                                           gCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCa         <  1:39313/150‑1 (MQ=255)
                                                                                                                                                ggTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGc    >  1:1319775/1‑150 (MQ=255)
                                                                                                                                                  tGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGa  >  2:1039246/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGA  >  NC_000913/4593283‑4593578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: