Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,593,434 C→T 6.5% C430Y (TGC→TAC) ‡ lgoT ← putative L‑galactonate:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,4340CT 6.5% 340.2 / 8.2 108C430Y (TGC→TAC) ‡lgoTputative L‑galactonate:H+ symporter
Reads supporting (aligned to +/- strand):  ref base C (46/55);  new base T (2/5);  total (48/60)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 108 total aligned reads displayed.

CGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGT  >  NC_000913/4593288‑4593581
                                                                                                                                                  |                                                                                                                                                   
cGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAAc                                                                                                                                                  <  1:1309522/150‑1 (MQ=255)
    gagaACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCa                                                                                                                                              <  1:1534292/150‑1 (MQ=255)
      gaACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgc                                                                                                                                            <  1:371092/150‑2 (MQ=255)
       aaCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgcg                                                                                                                                           <  2:1385135/150‑3 (MQ=255)
        aCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgcgg                                                                                                                                          <  1:657784/150‑4 (MQ=255)
        aCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgatg                                                                                                                                          <  1:253973/150‑1 (MQ=255)
          tGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAgatgat                                                                                                                                        >  2:133145/1‑150 (MQ=255)
           gggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTTGCGTCACCGCAgcggggg                                                                                                                                       <  2:504640/150‑7 (MQ=255)
            ggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTa                                                                                                                                      >  1:1675392/1‑150 (MQ=255)
            ggAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTa                                                                                                                                      >  1:1056793/1‑150 (MQ=255)
               aaCATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTg                                                                                                                                   <  1:343460/150‑1 (MQ=255)
                 cATTTTAATCGCCATTAAAATGCCCGATAAGAAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGcc                                                                                                                                 <  2:276809/150‑1 (MQ=255)
                 cATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGcc                                                                                                                                 >  2:283208/1‑150 (MQ=255)
                   ttttAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAg                                                                                                                               >  2:410827/1‑150 (MQ=255)
                   ttttAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAg                                                                                                                               <  2:1023896/150‑1 (MQ=255)
                       aaTCTCGATGATAAAGCCCGATAAGCAAAATGATATCGGGCATAAGGAGAATAATCTTTACGTGCGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACgg                                                                                                                           <  1:253368/150‑1 (MQ=255)
                           gCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATg                                                                                                                       <  2:876854/150‑1 (MQ=255)
                                 aaaaTGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAgtggg                                                                                                                 <  2:417256/150‑1 (MQ=255)
                                    aTGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAgtgggtgg                                                                                                              <  1:1712212/150‑1 (MQ=255)
                                       cccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACTGAATGAGTGGGTGGTAt                                                                                                           >  1:1385349/1‑150 (MQ=255)
                                       cccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTAt                                                                                                           >  1:583810/1‑150 (MQ=255)
                                       cccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTAt                                                                                                           <  2:958322/150‑1 (MQ=255)
                                       cccGATAAGCAAAATCTTAACGGGCATAAGGAGATTAATCTATAAGTGGCTCGTTTATCGGCTCACGAACCAGGAAGATGTACGCCAGTGCCCACGCTGCGGTCACGCAACCGCAGAAGATTAGTGCCAGACGGAATGAGTGGGTGCTAt                                                                                                           >  1:872270/1‑150 (MQ=255)
                                        ccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATc                                                                                                          <  1:730828/150‑1 (MQ=255)
                                        ccGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATc                                                                                                          <  2:416461/150‑1 (MQ=255)
                                           aTAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaac                                                                                                       <  2:1131795/150‑1 (MQ=255)
                                            tAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaaca                                                                                                      <  1:783703/150‑1 (MQ=255)
                                             aaGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATcaacaa                                                                                                     <  2:185578/150‑1 (MQ=255)
                                              aGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAAt                                                                                                    <  1:1657686/150‑1 (MQ=255)
                                              aGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAAt                                                                                                    >  1:935518/1‑150 (MQ=255)
                                                cAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGGCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATaa                                                                                                  <  2:885161/150‑1 (MQ=255)
                                                 aaaaTGTTATCGGTCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACAGCAGATGATTAGTGCCAGACGGAATGAGTGGGGGGTATCAACAATaaa                                                                                                 <  1:1488634/150‑1 (MQ=255)
                                                 aaaaTGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGGTGCTCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTACTGCCAGACGGAAGGAGTGGGTCGGAGCAACAAGaaa                                                                                                 >  1:47655/1‑150 (MQ=255)
                                                   aaTGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAAc                                                                                               >  1:1627622/1‑150 (MQ=255)
                                                      tttATCGGGCATAAGGAGATTAATCTTTACGTGGGGCGGTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGGCGCAAACGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAg                                                                                            <  2:851387/149‑1 (MQ=255)
                                                       ttttCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGt                                                                                           <  2:1276844/147‑1 (MQ=255)
                                                        tATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTa                                                                                          >  1:1244980/1‑150 (MQ=255)
                                                         aTCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTaa                                                                                         >  1:1534046/1‑150 (MQ=255)
                                                             ggCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGAt                                                                                     >  2:713032/1‑150 (MQ=255)
                                                              gCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATc                                                                                    <  1:1600891/150‑1 (MQ=255)
                                                              gCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATc                                                                                    <  2:1675392/150‑1 (MQ=255)
                                                               cATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCg                                                                                   <  2:430079/150‑1 (MQ=255)
                                                               cATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCg                                                                                   <  2:407923/150‑1 (MQ=255)
                                                                 tAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgc                                                                                 <  2:1488495/150‑1 (MQ=255)
                                                                 tAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgc                                                                                 <  2:1221329/150‑1 (MQ=255)
                                                                  aaGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcg                                                                                <  2:1457524/150‑1 (MQ=255)
                                                                   aGGAGATTATGCTTTACGTGGGTCGTTGATCGGCTGACGACCCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGgcgc                                                                               <  2:47655/150‑1 (MQ=255)
                                                                    ggAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTTGTATCAACAATAAAACCAGTAATGATCGGCGCa                                                                              <  1:1240307/150‑1 (MQ=255)
                                                                         ttAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAaga                                                                         >  2:1141912/1‑150 (MQ=255)
                                                                           aaTCTTTACGTGGGGCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAgg                                                                       <  2:1341402/150‑1 (MQ=255)
                                                                              cTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGgcgc                                                                    >  2:1536963/1‑150 (MQ=255)
                                                                                ttACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAg                                                                  >  1:285823/1‑150 (MQ=255)
                                                                                   cGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATg                                                               >  2:638784/1‑150 (MQ=255)
                                                                                    gtgtgtCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCGCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGa                                                              <  2:266387/150‑1 (MQ=255)
                                                                                     tGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATgga                                                             >  2:581394/1‑148 (MQ=255)
                                                                                       ggTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGc                                                           <  1:1141912/150‑1 (MQ=255)
                                                                                         tCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTg                                                         <  2:1432265/150‑1 (MQ=255)
                                                                                         tCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTg                                                         <  1:133145/150‑1 (MQ=255)
                                                                                          cGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTgg                                                        <  2:1627622/150‑1 (MQ=255)
                                                                                           ggtGATCTGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGGGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGc                                                       <  2:1607631/148‑1 (MQ=255)
                                                                                           gTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGc                                                       <  2:581886/150‑1 (MQ=255)
                                                                                           gTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGc                                                       <  1:646184/150‑1 (MQ=255)
                                                                                             tGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATAAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCaa                                                     >  1:1612147/1‑150 (MQ=255)
                                                                                              gATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCaaa                                                    >  1:1709790/1‑150 (MQ=255)
                                                                                                 cGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGtt                                                 >  2:823136/1‑150 (MQ=255)
                                                                                                  ggCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGtta                                                >  1:617476/1‑149 (MQ=255)
                                                                                                    cTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAGGAGGCGCAGATGAAGCTGGCAAAGTTCTg                                              <  1:492558/150‑1 (MQ=255)
                                                                                                     tGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTgg                                             <  2:1244980/150‑1 (MQ=255)
                                                                                                      gACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGa                                            <  1:1252889/150‑1 (MQ=255)
                                                                                                        cGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATa                                          >  1:1630602/1‑150 (MQ=255)
                                                                                                          aaCCAGGTAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACt                                        <  1:1273174/150‑1 (MQ=255)
                                                                                                          aaCCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACt                                        <  2:935518/150‑1 (MQ=255)
                                                                                                           aCCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTg                                       >  2:1701346/1‑150 (MQ=255)
                                                                                                               ggAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCa                                   >  2:1522045/1‑150 (MQ=255)
                                                                                                                 aaGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGTATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCAcc                                 >  1:487311/1‑150 (MQ=255)
                                                                                                                 aaGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCAcc                                 >  1:816249/1‑150 (MQ=255)
                                                                                                                  aGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGGGGGATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCAccc                                >  1:110691/1‑149 (MQ=255)
                                                                                                                    aTGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGAc                              <  2:1056793/150‑1 (MQ=255)
                                                                                                                    aTGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGAc                              <  1:638784/150‑1 (MQ=255)
                                                                                                                     tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCTCCGACg                             >  1:315803/1‑150 (MQ=255)
                                                                                                                     tGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACg                             <  1:713032/150‑1 (MQ=255)
                                                                                                                       tACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCa                           >  2:1665969/1‑150 (MQ=255)
                                                                                                                           ccAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCa                       <  1:410827/150‑1 (MQ=255)
                                                                                                                             aGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATg                     >  2:39313/1‑150 (MQ=255)
                                                                                                                             aGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATg                     >  2:1179016/1‑150 (MQ=255)
                                                                                                                              gTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGc                    >  1:530358/1‑150 (MQ=255)
                                                                                                                                 cccccGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGTCATGCgag                 >  1:456204/1‑150 (MQ=255)
                                                                                                                                 aatcatcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGCCGCAGTCATGCgag                 >  1:381943/7‑150 (MQ=255)
                                                                                                                                     cGCTTCGGTGACGCAACCTCAGATGATTAGGGCCAGACGGAATGAGTGGGTGGTATCAAAAATATAACCTGTTATGATCGGCGCATAAGAGGCGCAGAGGAAGCTGGCAATGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAgg             <  2:872270/150‑2 (MQ=255)
                                                                                                                                     cGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATATTGCCCACCGACGCAGTCATGCGCGAAGc             >  2:320943/1‑150 (MQ=255)
                                                                                                                                     cGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGc             <  2:1330840/150‑1 (MQ=255)
                                                                                                                                     cGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGc             <  2:392466/150‑1 (MQ=255)
                                                                                                                                      tcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCt                                                          <  2:86675/103‑1 (MQ=255)
                                                                                                                                      tcTGCGGTTGCGTCACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCt                                                          >  1:86675/2‑104 (MQ=255)
                                                                                                                                      gCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCa            <  1:39313/150‑1 (MQ=255)
                                                                                                                                      gCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCa            >  1:995188/1‑150 (MQ=255)
                                                                                                                                           ggTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGc       >  1:1319775/1‑150 (MQ=255)
                                                                                                                                             tGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGa     >  2:1039246/1‑150 (MQ=255)
                                                                                                                                                cGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGt  >  1:1358825/1‑150 (MQ=255)
                                                                                                                                                cGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGt  >  2:20608/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGTGACGCAACCGCAGATGATTAGTGCCAGACGGAATGAGTGGGTGGTATCAACAATAAAACCAGTAATGATCGGCGCAAAAGAGGCGCAGATGAAGCTGGCAAAGTTCTGGATACTGCCCACCGACGCAGTCATGCGAGAAGCAACTGCGACGT  >  NC_000913/4593288‑4593581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: