Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,639,554 A→T 9.7% intergenic (+24/+36) creD → / ← arcA inner membrane protein/response regulator in two‑component regulatory system with ArcB or CpxA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,639,5540AT 9.7% 318.0 / 18.9 123intergenic (+24/+36)creD/arcAinner membrane protein/response regulator in two‑component regulatory system with ArcB or CpxA
Reads supporting (aligned to +/- strand):  ref base A (62/49);  new base T (7/5);  total (69/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 125 total aligned reads displayed.

CTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACG  >  NC_000913/4639405‑4639701
                                                                                                                                                     |                                                                                                                                                    
       tggCTCTGGCCGGAATGATTTTTGTTACCCGTAATATCTACTGTTATGCGTTTTCACTGCCTAAAATGAAAGCCATAAATGAAGTTACAACGGACGATGATTTACGTTTCTGTAAATAAGGTTTAAAAATAAAAACGGCGCTAAAAAgcg                                                                                                                                               <  1:1306163/150‑1 (MQ=255)
       tggCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAgcg                                                                                                                                               <  2:455742/150‑1 (MQ=255)
        ggCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAATCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAgcgc                                                                                                                                              <  1:1021081/150‑1 (MQ=255)
          cgcTGGCCGGCATGATTTTTTTTACACTTAATATCGACTTGTTTGCTTTTTAACTGCCTAAAATTAAAGCCAGTAAAGATTTTACAACGGACGATGTGTTACGTATCTGGAAATAAGGTTGAAAAAAAAAAACGGCGCTAAAAAGCGCCg                                                                                                                                            <  1:878693/150‑1 (MQ=255)
            gtGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAATTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAATACGGCGCTTTTTAGCGCCGtt                                                                                                                                         <  2:1227178/149‑1 (MQ=255)
            cTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGtt                                                                                                                                         >  2:68289/1‑150 (MQ=255)
             tGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGttt                                                                                                                                        >  2:1336364/1‑150 (MQ=255)
              ggCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGGCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTTTTTAGCGCCGtttt                                                                                                                                       <  2:908927/150‑1 (MQ=255)
               gCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTTTTTAGCGCCGttttt                                                                                                                                      <  1:688767/150‑1 (MQ=255)
                ccGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGtttttt                                                                                                                                     >  1:1430832/1‑150 (MQ=255)
                  ggCGTGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGtttttttt                                                                                                                                   >  2:1618655/1‑150 (MQ=255)
                  ggCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGtttttttt                                                                                                                                   >  2:607036/1‑150 (MQ=255)
                    cATGAGGTTTGTGACCCGAAAAATCGACTGGTATGCGTTTTAACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGa                                                                                                                                 <  2:1386750/150‑1 (MQ=255)
                      tgatgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACg                                                                                                                               >  1:1027639/1‑150 (MQ=255)
                      tgatgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACg                                                                                                                               >  2:873128/1‑150 (MQ=255)
                      tgatgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCAGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACg                                                                                                                               >  1:358265/1‑150 (MQ=255)
                       gatgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACgg                                                                                                                              >  1:632350/1‑150 (MQ=255)
                        atgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTTTTTAGCGCCGtttttatttttcaa                                                                                                                             <  2:333978/150‑7 (MQ=39)
                          gTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACgttgg                                                                                                                           >  2:1309008/1‑150 (MQ=255)
                             tgtgACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACTGTTGTaa                                                                                                                        >  2:1406921/1‑150 (MQ=255)
                              gtgACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGTAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTaaa                                                                                                                       >  2:903455/1‑150 (MQ=255)
                              gtgACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAACCGGCGCTAAAAAGCTCCGGTTTTTTTGACGGTGGTaaa                                                                                                                       >  1:308479/1‑150 (MQ=255)
                               tgACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAg                                                                                                                      <  2:534662/150‑1 (MQ=255)
                                gACCCGTAATATCGACTGGTATGTGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGc                                                                                                                     >  1:660012/1‑150 (MQ=255)
                                 aCCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGcc                                                                                                                    >  2:876435/1‑150 (MQ=255)
                                   ccGTAATATCGACTGGTATGCGTTATCACTGCCCAAAATGAAACCCAGTAACGAAGTTACAACGCACGATGAGTTACGTAGCTGGAAATACGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGa                                                                                                                  <  1:1620253/150‑1 (MQ=255)
                                        atatCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAAt                                                                                                             <  2:3390/150‑1 (MQ=255)
                                         tatCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAAta                                                                                                            >  2:1641485/1‑149 (MQ=255)
                                          atCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCt                                                                                                           >  2:1677420/1‑150 (MQ=255)
                                             gACTGGTATGCGTTTTCAATGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTcc                                                                                                        <  1:820436/150‑1 (MQ=255)
                                              aCTGGTATGTGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCa                                                                                                       <  2:660012/150‑1 (MQ=255)
                                              aCTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTTACGGGTGTAAAGCCGATTAATATTCCa                                                                                                       >  1:1650938/1‑150 (MQ=255)
                                                 ggTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGAt                                                                                                    >  1:1261420/1‑150 (MQ=255)
                                                  ttATGCTTTTTCACTCCCGAAAATTAAAGCCAGAAAATATGTAAAAACGGACGATGATTTACGTATCTGTAAATAAGTTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATc                                                                                                   <  1:607036/149‑1 (MQ=255)
                                                  gTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATc                                                                                                   <  1:1336364/150‑1 (MQ=255)
                                                      gCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCg                                                                                               <  1:1618655/150‑1 (MQ=255)
                                                       cGTTTTCACTGCCTAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGc                                                                                              <  2:1281427/150‑1 (MQ=255)
                                                       cGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGc                                                                                              >  2:1330375/1‑150 (MQ=255)
                                                        tttttCACTGCCTAAAATGAAAGCCATTAAAGAAGTTACAACGGACTATGATTAACGTATCTGGAAATAAGGTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCa                                                                                             <  1:1252830/149‑1 (MQ=255)
                                                        gTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCa                                                                                             >  2:1326875/1‑150 (MQ=255)
                                                         ttttCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAg                                                                                            <  1:972653/150‑1 (MQ=255)
                                                             cACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGc                                                                                        >  1:1264592/1‑150 (MQ=255)
                                                               cTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGa                                                                                      >  1:19740/1‑150 (MQ=255)
                                                               cTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTATTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGa                                                                                      >  2:900540/1‑150 (MQ=255)
                                                                 gCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATa                                                                                    >  2:668050/1‑150 (MQ=255)
                                                                 gCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATATTCCAGATCACCGCAGAAGCGATa                                                                                    >  1:1662462/1‑150 (MQ=255)
                                                                   cGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAAc                                                                                  >  1:112963/1‑150 (MQ=255)
                                                                    gAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAAcc                                                                                 <  1:237941/150‑1 (MQ=255)
                                                                      aaaTGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggtgg                                                                                                                           <  2:1485345/106‑1 (MQ=255)
                                                                      aaaTGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggtgg                                                                                                                           >  1:1485345/1‑106 (MQ=255)
                                                                         tGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCAc                                                                            >  1:1121947/1‑150 (MQ=255)
                                                                          gAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCAcc                                                                           >  1:455363/1‑150 (MQ=255)
                                                                              tccAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGa                                                                       <  1:1038293/149‑1 (MQ=255)
                                                                               ccAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGaa                                                                      <  2:1179819/150‑1 (MQ=255)
                                                                                            tACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTATTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGTCgatgat                                                         >  2:733527/1‑150 (MQ=255)
                                                                                             aCAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGAtt                                                        >  1:1667529/1‑150 (MQ=255)
                                                                                              cAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGAttt                                                       <  1:303193/150‑1 (MQ=255)
                                                                                                ccgcaCGATGAGTTACGTATATTGAAATAAGTTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtcc                                                     <  1:1714243/146‑1 (MQ=255)
                                                                                                  ggACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccgg                                                   <  1:999972/150‑1 (MQ=255)
                                                                                                  ggACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccgg                                                   <  1:1152838/150‑1 (MQ=255)
                                                                                                    aCGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccggcg                                                 <  2:388596/150‑1 (MQ=255)
                                                                                                     cGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccggcgt                                                >  2:239061/1‑150 (MQ=255)
                                                                                                       aTGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccggcgtat                                              <  1:31624/150‑1 (MQ=255)
                                                                                                         gAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTTACGGTTGTAAATCCGATTAATAATACAGATAACCGCAGAAGCGAATACCTTAACCGTGAATTGTTGCGTTTAATtacggcgtatac                                            >  1:361414/1‑148 (MQ=255)
                                                                                                          aGTTACGTATCAGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCTACGTTTTTTTTGAAGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAACCGATAAACTTCAAAGTGAATGGTGGCGATGATTtccgccgtaaccg                                           >  1:1449953/1‑150 (MQ=255)
                                                                                                           gTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccggcgtatccgg                                          >  1:546167/1‑150 (MQ=255)
                                                                                                           gTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccggcgtatccgg                                          <  1:68289/150‑1 (MQ=255)
                                                                                                            ttACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccggcgtatccggc                                         <  2:369620/150‑1 (MQ=255)
                                                                                                             tACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccggcgtatccggcg                                        >  2:77456/1‑150 (MQ=255)
                                                                                                               cGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTtccggcgtatccggcgta                                      >  1:1367437/1‑150 (MQ=255)
                                                                                                                 tATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGa                                    >  2:1664922/1‑150 (MQ=255)
                                                                                                                  aTCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCCCCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGAt                                   >  1:786650/1‑150 (MQ=255)
                                                                                                                  aTCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGAt                                   <  1:640274/150‑1 (MQ=255)
                                                                                                                  aTCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGAt                                   >  1:1693592/1‑150 (MQ=255)
                                                                                                                  aTCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGAt                                   >  2:476390/1‑150 (MQ=255)
                                                                                                                    cTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTGCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTc                                 >  2:1565731/1‑150 (MQ=255)
                                                                                                                    cTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTc                                 >  2:896794/1‑150 (MQ=255)
                                                                                                                       gAAATAAGGTTGAAAAATTAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCATGCGTATCCTGCGTAGATTCGaa                              <  2:241534/150‑1 (MQ=255)
                                                                                                                       gAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGaa                              <  1:1173713/150‑1 (MQ=255)
                                                                                                                           tAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGt                          >  1:1314906/1‑150 (MQ=255)
                                                                                                                            aaGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTGTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGATTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGATATGtt                         >  1:494946/1‑150 (MQ=255)
                                                                                                                             tggTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGttt                        <  1:756113/149‑1 (MQ=255)
                                                                                                                             aGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGATGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGtt                         >  2:218501/1‑150 (MQ=255)
                                                                                                                             aGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTACAACGTGAATGGTGGAGATGATTTCCGGCGTATCCGGGGGAGATGCGAAATGtt                         >  2:213279/1‑150 (MQ=255)
                                                                                                                                 tGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGa                    >  2:336571/1‑150 (MQ=255)
                                                                                                                                     aaaTAAACACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAc                <  1:668050/150‑1 (MQ=255)
                                                                                                                                     aaaTAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAc                <  2:455363/150‑1 (MQ=255)
                                                                                                                                      aaaaaaaaCGGCGCTTTTTAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcg               >  1:984350/4‑150 (MQ=255)
                                                                                                                                      aaTAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATTATTTCCGGCGTAACCGGCGTAGATACGAAATGTTTACGAATAcg               >  2:1374401/1‑150 (MQ=255)
                                                                                                                                      aaTAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcg               <  2:1416730/150‑1 (MQ=255)
                                                                                                                                        tAAAAACGGCGCTAAAAAGCGCGGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTAGAAATGTTTACGAATAcggc             >  2:1216013/1‑150 (MQ=255)
                                                                                                                                          aaaaCGGCGCTTTTTAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcggcgg           >  2:1569905/1‑150 (MQ=255)
                                                                                                                                          aaaaCGGCGCTAAAAAGCGCCGTTCTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGAGGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcggcgg           <  1:1120041/150‑1 (MQ=255)
                                                                                                                                           aaaCGGCGCTTTTTAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGa          >  1:1100512/1‑150 (MQ=255)
                                                                                                                                            aaCGGCGCTTTTTAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGAt         >  2:799541/1‑150 (MQ=255)
                                                                                                                                              cGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCg       <  2:1430832/150‑1 (MQ=255)
                                                                                                                                              cGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGCGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCg       >  1:794962/1‑150 (MQ=255)
                                                                                                                                                 cgcTAAAAAGCGCGGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCa    <  1:1546709/150‑1 (MQ=255)
                                                                                                                                                 cgcTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACAAATACGGCGGATCGTCa    <  2:505095/150‑1 (MQ=255)
                                                                                                                                                   cTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACg  >  2:344445/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACG  >  NC_000913/4639405‑4639701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: