Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,639,901 T→G 5.4% S136R (AGC→CGC)  arcA ← response regulator in two‑component regulatory system with ArcB or CpxA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,639,9010TG 5.4% 312.4 / 5.6 105S136R (AGC→CGC) arcAresponse regulator in two‑component regulatory system with ArcB or CpxA
Reads supporting (aligned to +/- strand):  ref base T (47/52);  new base G (3/3);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

CAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACT  >  NC_000913/4639752‑4640049
                                                                                                                                                     |                                                                                                                                                    
cagTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCt                                                                                                                                                      <  1:35922/150‑1 (MQ=255)
cagTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCt                                                                                                                                                      >  2:171805/1‑150 (MQ=255)
  gTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGCGCCGATCAACGAACGGCTGTTGATGTCCAGGTCCCCACCATTGAACTTGTAGCttt                                                                                                                                                    >  1:1710348/1‑150 (MQ=255)
      aGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTTCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGCTGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAAc                                                                                                                                                >  1:251520/1‑150 (MQ=255)
      aGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAAc                                                                                                                                                <  1:119452/150‑1 (MQ=255)
        cACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGc                                                                                                                                              <  2:1372458/150‑1 (MQ=255)
            ggACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTacg                                                                                                                                          >  1:1141428/1‑150 (MQ=255)
            ggACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTacg                                                                                                                                          >  2:562037/1‑150 (MQ=255)
            ggACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTacg                                                                                                                                          >  1:60500/1‑150 (MQ=255)
              aCTGAATTTTGCATGGGTTTTCTCAGTAGTGTAGCATGGCGCGGAAGTCGCTGCGCGGCAGCTTGTACTGCTCGCCTTCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCTTCGCTacgac                                                                                                                                        <  2:1449953/150‑1 (MQ=255)
               cTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTacga                                                                                                                                         >  1:69348/1‑148 (MQ=255)
               cTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTacga                                                                                                                                         <  2:69348/148‑1 (MQ=255)
                 gAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCGTTGAAAGCTacaagttc                                                                                                                                     <  1:823095/150‑7 (MQ=255)
                 gAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGACGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCGTTGAAAGCTacaagttc                                                                                                                                     <  1:954645/150‑7 (MQ=255)
                  aaTTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCGTTGAAAGCTacaagttca                                                                                                                                    <  2:530490/150‑8 (MQ=255)
                        gCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCt                                                                                                                              >  2:1682093/1‑150 (MQ=255)
                        gCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCt                                                                                                                              >  2:374023/1‑150 (MQ=255)
                         ccTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTg                                                                                                                             >  1:194004/1‑150 (MQ=255)
                          cTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTgc                                                                                                                            >  1:155287/1‑149 (MQ=255)
                           tGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGAc                                                                                                                           <  1:1716039/150‑1 (MQ=255)
                             ggTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAg                                                                                                                         <  1:555769/150‑1 (MQ=255)
                             ggTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAg                                                                                                                         <  1:548994/150‑1 (MQ=255)
                             ggTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAg                                                                                                                         <  2:1173786/150‑1 (MQ=255)
                                tttCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAc                                                                                                                      <  1:1472189/150‑1 (MQ=255)
                                tttCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCGGGTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAc                                                                                                                      <  2:1100512/150‑1 (MQ=255)
                                 ttCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCGTTCAACGCTACGACGTTCTTCGCTGAAAGTAcc                                                                                                                     >  2:1252966/1‑150 (MQ=255)
                                  tCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAccc                                                                                                                    <  2:1667529/150‑1 (MQ=255)
                                   cacaGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCa                                                                                                                   >  2:296870/1‑150 (MQ=255)
                                     caGAAGTGATGCATGGCGCGGAACTCGCTGCGCGGCAGCTGGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCATCGCTGACAGTACCCAGa                                                                                                                 <  2:652597/150‑1 (MQ=255)
                                     caGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGa                                                                                                                 >  2:673858/1‑150 (MQ=255)
                                           tGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATg                                                                                                           <  1:402274/150‑1 (MQ=255)
                                            gAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATgg                                                                                                          <  2:687979/150‑1 (MQ=255)
                                            gAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATgg                                                                                                          <  2:739364/150‑1 (MQ=255)
                                             atgCATGTCGCGGAACTCGCGGCGCGGCAGCTTGTACTGATCGACATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTATCTTTCATCTATACGACGTTCTTCGCTGACAGTACCCAGATTCATGGt                                                                                                         <  1:1158190/148‑1 (MQ=255)
                                                cATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACg                                                                                                      >  2:637390/1‑150 (MQ=255)
                                                 aTGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACgg                                                                                                     >  1:1640988/1‑150 (MQ=255)
                                                    gcgcGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGAc                                                                                                  <  1:171805/150‑1 (MQ=255)
                                                    gcgaGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCAAGTTACCAACCATTGAAATTGTAGCTTTCAACGCTACGTCGTTCTTAGCTGACAGTACCCAGATTCATGGTACGGGAc                                                                                                  <  1:1580586/150‑1 (MQ=255)
                                                       cgGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGATACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGt                                                                                               <  1:1690002/150‑1 (MQ=255)
                                                        ggAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTa                                                                                              >  1:1436327/1‑150 (MQ=255)
                                                        ggAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTa                                                                                              <  1:155611/150‑1 (MQ=255)
                                                          aaCTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCACCGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAgg                                                                                            <  2:1374053/150‑1 (MQ=255)
                                                           aCTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGt                                                                                           <  2:250564/150‑1 (MQ=255)
                                                           aCTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGt                                                                                           <  2:546167/150‑1 (MQ=255)
                                                             tCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTg                                                                                         >  1:1087919/1‑150 (MQ=255)
                                                                cTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATAAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTATCAACGCTACGACGTACTTCGCTGCCAGTACCCAGATACATGCTACGGGACAGTAGGTTGCGt                                                                                      >  1:175740/1‑150 (MQ=255)
                                                                 tGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTg                                                                                     <  2:1457725/150‑1 (MQ=255)
                                                                  gcgcGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCGCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTTCGTGc                                                                                    >  2:1288220/1‑150 (MQ=255)
                                                                        cAGCTTGTACTGCTCGACATCAGGGCCGATCAACGAACTGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACATTACCCATTTTCATGGTACGGGACAGTAGGTTGCGTGCACGAAt                                                                              <  1:1324330/150‑1 (MQ=255)
                                                                         aGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAATGCTACGACGTTCTGCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATc                                                                             >  2:1101449/1‑150 (MQ=255)
                                                                            ttGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTc                                                                          <  1:374023/150‑1 (MQ=255)
                                                                            ttGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTGCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTc                                                                          <  1:562037/150‑1 (MQ=255)
                                                                               tACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGt                                                                                           <  2:286038/130‑1 (MQ=255)
                                                                               tACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGt                                                                                           >  1:286038/1‑130 (MQ=255)
                                                                                 cggCTAGCCATAAGGGCCGATCAACGGACGGCTGTTGATGTCCAGGTACCAACCAATGCACATGTTGCTTTCAACGCTAAGTCGTTATTCGCTGACAGTTACCAGAATCATGGTTCGGGGCAGTTGGTTGCGTGCACGAATCGTCAGttt                                                                     >  2:1488457/3‑149 (MQ=255)
                                                                                 cTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTc                                                                     >  1:487182/1‑150 (MQ=255)
                                                                                  tGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCa                                                                    <  1:1358545/150‑1 (MQ=255)
                                                                                    cTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTAAcg                                                                  >  1:1500584/1‑150 (MQ=255)
                                                                                      cGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCAcgcg                                                                >  1:1716279/1‑150 (MQ=255)
                                                                                      cGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCAcgcg                                                                <  2:194004/150‑1 (MQ=255)
                                                                                      cGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCAAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCAcgcg                                                                >  2:1490748/1‑150 (MQ=255)
                                                                                       gCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCgg                                                               >  1:427437/1‑150 (MQ=255)
                                                                                       cccATCAGGGCCGATCACCGAACGGCTGTTGATGTCCAGTTCCCAACCATTGACCTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCTGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCgg                                                               <  2:300788/149‑1 (MQ=255)
                                                                                              gggCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAAc                                                        <  2:60500/150‑1 (MQ=255)
                                                                                                gCCGATCAACGAACGGCGGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACgg                                                      <  2:394352/150‑1 (MQ=255)
                                                                                                   gATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACTGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGttt                                                   >  2:1277041/1‑150 (MQ=255)
                                                                                                   gATCAACGAACGGCGGTTGATGTCCTGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGttt                                                   <  1:327839/150‑1 (MQ=255)
                                                                                                     tCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTCAACTTGTAGCTTTCAACGCTACGTCGTTCTGCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGTACGGTTTgg                                                 <  1:296870/150‑1 (MQ=255)
                                                                                                       aacgaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTg                                               <  2:219629/150‑1 (MQ=255)
                                                                                                        acgaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGa                                              <  1:673858/150‑1 (MQ=255)
                                                                                                         cgaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGAt                                             <  2:1436327/150‑1 (MQ=255)
                                                                                                          gaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATg                                            >  1:805993/1‑150 (MQ=255)
                                                                                                          gaacgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATg                                            >  1:799272/1‑150 (MQ=255)
                                                                                                             cgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAg                                         >  1:770616/1‑150 (MQ=255)
                                                                                                             cgGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGATCACGCGGGTTGAACGGTTTGGTGATGTAg                                         >  2:44677/1‑150 (MQ=255)
                                                                                                               gCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGGTGACAGTACCCTGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGAGATGTTGTc                                       >  2:362323/1‑150 (MQ=255)
                                                                                                                cTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCa                                      <  2:1087919/150‑1 (MQ=255)
                                                                                                                 tGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCAt                                     >  1:1122177/1‑150 (MQ=255)
                                                                                                                 ggTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCAt                                     <  1:637390/149‑1 (MQ=255)
                                                                                                                  gTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATc                                    >  2:122426/1‑150 (MQ=255)
                                                                                                                   ttGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCt                                   >  2:17886/1‑150 (MQ=255)
                                                                                                                    tGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTg                                  <  1:519102/150‑1 (MQ=255)
                                                                                                                     gATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGc                                 >  1:839659/1‑150 (MQ=255)
                                                                                                                        gTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCAcc                              <  2:427437/150‑1 (MQ=255)
                                                                                                                          ccAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGa                            >  1:17794/1‑150 (MQ=255)
                                                                                                                               tCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCg                       >  2:681461/1‑150 (MQ=255)
                                                                                                                               tCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCg                       >  1:682594/1‑150 (MQ=255)
                                                                                                                                cccAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGa                      >  2:627894/1‑150 (MQ=255)
                                                                                                                                  cAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAgg                    >  2:811765/1‑150 (MQ=255)
                                                                                                                                  cAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAgg                    <  2:858055/150‑1 (MQ=255)
                                                                                                                                   aaCCATTGAACTTATAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGc                   <  2:537669/150‑1 (MQ=255)
                                                                                                                                       aTTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCgag               <  2:1593620/150‑1 (MQ=255)
                                                                                                                                         tGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTTCCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGaa             <  1:327952/150‑1 (MQ=255)
                                                                                                                                           aaCTTGTAGCTTTCAACGCTACGACGTTGTTAGCTGACAGTACCCAGATTCATGGTACGGTACAGTAGGTTGCGTGCACGAATCGTCTGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAtt           <  2:175740/150‑1 (MQ=255)
                                                                                                                                            cgtcgTAGCGTTGAAAGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAAAGGTTTGGTGATGTAGTCATCTGCACAGATTTCGAGGCCGTGAtttt          >  2:308301/5‑150 (MQ=255)
                                                                                                                                             gtcgTAGCGTTGAAAGCTACGACGTTCTTCGCTGACAGTTCCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAAtttt         >  2:743990/4‑150 (MQ=255)
                                                                                                                                                gTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATc      >  2:1548269/1‑150 (MQ=255)
                                                                                                                                                gTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATc      >  1:467034/1‑150 (MQ=255)
                                                                                                                                                   gCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGAc   >  1:334249/1‑150 (MQ=255)
                                                                                                                                                    cTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACt  >  1:65291/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTTGATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGACAGTAGGTTGCGTGCACGAATCGTCAGTTCACGCGGGTTGAACGGTTTGGTGATGTAGTCATCTGCACCGATTTCGAGGCCGAGAATTTTATCGACT  >  NC_000913/4639752‑4640049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: