Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 506,586 T→A 5.7% intergenic (+20/+17) ushA → / ← ybaK bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913506,5860TA 5.7% 285.0 / 4.5 98intergenic (+20/+17)ushA/ybaKbifunctional UDP‑sugar hydrolase/5'‑nucleotidase/Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase
Reads supporting (aligned to +/- strand):  ref base T (46/45);  new base A (2/4);  total (49/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CCTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCG  >  NC_000913/506437‑506735
                                                                                                                                                     |                                                                                                                                                     
ccTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGttt                                                                                                                                                       >  2:871259/1‑150 (MQ=255)
 cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                      <  2:2173/150‑1 (MQ=255)
 cTTGATAACAAACCCGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGAGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                      <  1:116976/150‑1 (MQ=255)
  ttGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGc                                                                                                                                                     >  1:1425746/1‑150 (MQ=255)
   tGATAACAAACCGGGCTAGGTGACTACCGGCTTTATTGAGGCCGAAGGGCTGAATGCGTATTTGCAGAAAAGCTGGCCGCTGGATGTGAGGGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCa                                                                                                                                                    <  1:1047568/150‑1 (MQ=255)
     aTAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTATATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATc                                                                                                                                                  <  1:307083/150‑1 (MQ=255)
         caacacGGTCTATGTGAATCACGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGATTGTTTATGAACCGAAATGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                              <  2:1142407/145‑1 (MQ=255)
           aaCCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGcac                                                                                                                                            <  1:472178/150‑1 (MQ=255)
           aaCCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGcac                                                                                                                                            <  1:1108860/150‑1 (MQ=255)
            aCCGTGCTATGTGAATAGCGGATTTATTGATGCCGAAGTGCTGAATGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTTAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGcact                                                                                                                                           <  1:1292046/150‑2 (MQ=255)
            aCCGGGCTATGTGAATACCGGCTTTATTGATGCCTAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGcaca                                                                                                                                           <  1:1295880/150‑1 (MQ=255)
            aCCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGcaca                                                                                                                                           <  2:949674/150‑1 (MQ=255)
               gggCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTGGCATCCGGCACAATg                                                                                                                                        >  2:845774/1‑150 (MQ=255)
               gggCTATGTGAATACAGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCTACGCTGGATGTGAGTGTTTATGAACAGAAAGGTGAGGTGAGCTGGCATTAATCCGAAAGTGCCGGATGATTGCATCCGGCACAATg                                                                                                                                        >  2:1357833/1‑150 (MQ=255)
                ggCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGc                                                                                                                                       <  1:588718/150‑1 (MQ=255)
                 gCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCt                                                                                                                                      <  1:22478/150‑1 (MQ=255)
                    aTGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTaa                                                                                                                                   >  1:1465961/1‑150 (MQ=255)
                      gtgAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATc                                                                                                                                 >  1:1207731/1‑150 (MQ=255)
                           tACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCgcggc                                                                                                                            >  2:1657537/1‑150 (MQ=255)
                           tACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCgcggc                                                                                                                            >  2:1657525/1‑150 (MQ=255)
                            aCCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCgcggcg                                                                                                                           >  1:241087/1‑150 (MQ=255)
                             ccGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGcggcgg                                                                                                                          >  2:1547771/1‑150 (MQ=255)
                               ggCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcgggc                                                                                                                        <  1:896008/150‑1 (MQ=255)
                                 cTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGAGGCGGGCGa                                                                                                                      >  2:1012014/1‑150 (MQ=255)
                                    tATTGATGCCGAAGTGCTGAATGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACATTGCTTAATCGCGGCGGGCGatat                                                                                                                   >  2:92949/1‑150 (MQ=255)
                                    tATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCAGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGatat                                                                                                                   <  2:1599327/150‑1 (MQ=255)
                                      ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                                 <  2:1425746/150‑1 (MQ=255)
                                        gATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGc                                                                                                               <  1:1657537/150‑1 (MQ=255)
                                        gATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGGGAGGTGAGCTGGCAGTAAGCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGc                                                                                                               <  2:49321/150‑1 (MQ=255)
                                         aTGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCa                                                                                                              >  2:396447/1‑150 (MQ=255)
                                          tGCCGAAGTCCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTTCCCGATGTTATCATCCGGAAAAATGCTAAATCGCGGCGGGCCATATCAGCaa                                                                                                             >  1:1097509/1‑150 (MQ=255)
                                            ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCt                                                                                                                                      >  1:423214/1‑123 (MQ=255)
                                            ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCt                                                                                                                                      <  2:423214/123‑1 (MQ=255)
                                            ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAt                                                                                                           >  2:228792/1‑150 (MQ=255)
                                                 gTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGc                                                                                                      >  1:818498/1‑150 (MQ=255)
                                                  tGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCa                                                                                                     <  2:1720646/150‑1 (MQ=255)
                                                   gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAATTGCCGGATGTTTGCATCCGGCACAATGCTTAATAGCGGCGGGCGATATCAGCAAATTTGGCAt                                                                                                    >  1:481683/1‑150 (MQ=255)
                                                   gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCAt                                                                                                    >  1:948221/1‑150 (MQ=255)
                                                   gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCAt                                                                                                    >  2:1519732/1‑150 (MQ=255)
                                                     ggAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCg                                                                                                  <  1:613347/149‑1 (MQ=255)
                                                        aaGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTgg                                                                                                       <  2:887228/142‑1 (MQ=255)
                                                        aaGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTgg                                                                                                       >  1:887228/1‑142 (MQ=255)
                                                         aGCGTATATCCAGAAAAGCTCGCCGCTGGATGTTAGTGTTTATGATCCGAAAGGTGAGGGGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGa                                                                                              <  1:1678378/150‑1 (MQ=255)
                                                         aGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGa                                                                                              >  1:1537185/1‑150 (MQ=255)
                                                            gTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCt                                                                                           >  2:1671778/1‑150 (MQ=255)
                                                             tataTCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATACGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCtt                                                                                          >  2:1181921/1‑150 (MQ=255)
                                                               tatCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGcac                                                                                                                                            <  1:1343454/98‑1 (MQ=255)
                                                               tatCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGcac                                                                                                                                            >  2:1343454/1‑98 (MQ=255)
                                                                atCCAGAAAAGCTCGCCGCTGGATTTGAGTGTTTATGAACAGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGc                                                                                       >  1:1607965/1‑150 (MQ=255)
                                                                 tCCAGAAAAGCTCTCAGCTGGTTGTGAGTGTTTTTTAACCGAAATGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGTATGTTTGCATCCGGCACAATGCTTAATCGCGGCTGGCGATATCAGCAATTTTGGCATCGAGGATCTTTGcc                                                                                      <  1:1357833/150‑1 (MQ=255)
                                                                 tCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGcc                                                                                      <  1:1448903/150‑1 (MQ=255)
                                                                   cAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCATTGCCAg                                                                                    <  1:228792/150‑1 (MQ=255)
                                                                       aattcTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCg                                                                                <  1:1412198/146‑1 (MQ=255)
                                                                       aaaGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCg                                                                                >  2:331930/1‑150 (MQ=255)
                                                                         aGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGcc                                                                              <  1:1616751/150‑1 (MQ=255)
                                                                          gCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCt                                                                             >  2:985326/1‑150 (MQ=255)
                                                                           cTCGACGCTGGATGTGAGTGTTTAAGAACCGAAAGGTGAGGTTAGCTGGCAGTCATCCGAAAGTGCCGGATGATTGCATCCGGCACAATGCTAACTCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTg                                                                            <  1:834586/150‑1 (MQ=255)
                                                                              gccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccg                                                                         >  1:733666/1‑150 (MQ=255)
                                                                              gccgcTGGAGGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccg                                                                         <  1:294130/150‑1 (MQ=255)
                                                                                    ggATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGtt                                                                   >  1:1345451/1‑150 (MQ=255)
                                                                                       tgtgtgTTTTTATGATCCTAAAGGTGAGGTTAGCTGTCAGTAAGCCGAATGTGCAGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCgt                                                                <  2:1097509/150‑2 (MQ=255)
                                                                                        gtgAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGat                                                               <  1:664327/150‑1 (MQ=255)
                                                                                         tgAGTGTTTATGAACCGAACGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGata                                                              <  2:288862/150‑1 (MQ=255)
                                                                                         tgAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGTTATCAGCAAATTTGGCATCGAGGATCTTTGCCACATCGCCTGCCGCCAGTTCGata                                                              >  2:1114363/1‑150 (MQ=255)
                                                                                         tgAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGata                                                              <  2:1174527/150‑1 (MQ=255)
                                                                                         ggAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGata                                                              <  1:920203/149‑1 (MQ=255)
                                                                                           aGTGTTTATGAACCGAACGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATc                                                            <  2:1420370/150‑1 (MQ=255)
                                                                                            gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                           <  1:1020758/150‑1 (MQ=255)
                                                                                            gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                           >  2:1617394/1‑150 (MQ=255)
                                                                                            gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                           <  1:871259/150‑1 (MQ=255)
                                                                                              gtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAg                                                         <  1:520399/150‑1 (MQ=255)
                                                                                                ttATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTc                                                       >  1:184823/1‑150 (MQ=255)
                                                                                                 tATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTcc                                                      <  1:974661/150‑1 (MQ=255)
                                                                                                  aTGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcg                                                     <  1:1657525/150‑1 (MQ=255)
                                                                                                  aTGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcg                                                     >  1:970621/1‑150 (MQ=255)
                                                                                                     aaCCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCgcgc                                                  >  2:33078/1‑150 (MQ=255)
                                                                                                     aaCCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCgcgc                                                  >  2:1192970/1‑150 (MQ=255)
                                                                                                      aCCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCt                                                 >  2:668160/1‑150 (MQ=255)
                                                                                                       ccGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCtt                                                >  1:1303289/1‑150 (MQ=255)
                                                                                                        cGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTg                                               >  2:387446/1‑150 (MQ=255)
                                                                                                         gAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTgc                                              >  1:896476/1‑150 (MQ=255)
                                                                                                           aaGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTgccg                                            >  1:1357695/1‑150 (MQ=255)
                                                                                                            aggtgaggtgAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTgccgc                                           <  2:1208132/150‑1 (MQ=255)
                                                                                                                     gagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACAt                                  <  1:1447706/150‑1 (MQ=255)
                                                                                                                       gCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATaa                                >  1:1372567/1‑150 (MQ=255)
                                                                                                                         tGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAAt                              >  1:51191/1‑150 (MQ=255)
                                                                                                                          ggCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGCTATCCAGTCCGCGCTTGCCGCCGGAAACATAAATa                             >  1:1289032/1‑150 (MQ=255)
                                                                                                                          ggCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATa                             >  2:31527/1‑150 (MQ=255)
                                                                                                                          ggCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATa                             <  2:241087/150‑1 (MQ=255)
                                                                                                                             aGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTg                          <  2:1465961/150‑1 (MQ=255)
                                                                                                                                     gAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAAttg                  >  1:655632/1‑149 (MQ=255)
                                                                                                                                       aaGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCtt                <  2:1537185/150‑1 (MQ=255)
                                                                                                                                       aaGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCtt                <  1:331930/150‑1 (MQ=255)
                                                                                                                                         gTGCCGGATGCAAACATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgt              >  1:905659/1‑150 (MQ=255)
                                                                                                                                          tGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgtg             <  2:1345451/150‑1 (MQ=255)
                                                                                                                                           gCCGGATGTTTGCATCCGGCACAATGCTTTATCGAGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGATCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGAGGCAAATTCTTGTGc            >  2:199631/1‑150 (MQ=255)
                                                                                                                                            ccGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCg           >  2:376832/1‑150 (MQ=255)
                                                                                                                                                aTGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCggggg       <  2:1372567/150‑1 (MQ=255)
                                                                                                                                                     tGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCg  >  2:1570983/1‑150 (MQ=255)
                                                                                                                                                     tGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCg  >  2:361827/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTCG  >  NC_000913/506437‑506735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: