Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,020 T→A 5.9% intergenic (+24/+12) cysS → / ← ybcI cysteinyl‑tRNA synthetase/DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,0200TA 5.9% 298.8 / 4.8 100intergenic (+24/+12)cysS/ybcIcysteinyl‑tRNA synthetase/DUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (47/47);  new base A (3/3);  total (50/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 104 total aligned reads displayed.

CAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATC  >  NC_000913/555871‑556164
                                                                                                                                                     |                                                                                                                                                
cAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGtt                                                                                                                                                  >  2:1467690/1‑150 (MQ=255)
 aaCAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGTTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGttt                                                                                                                                                 >  2:15781/1‑150 (MQ=255)
   cAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTc                                                                                                                                               >  2:1561381/1‑150 (MQ=255)
    cgCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCg                                                                                                                                              <  1:1468155/149‑1 (MQ=255)
    aGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTATCGAGATGGGGCTCGTGCTGGAAGATGGCCCGCAAGGGTCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCg                                                                                                                                              <  1:79204/150‑1 (MQ=255)
      cGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCa                                                                                                                                            >  2:1293273/1‑150 (MQ=255)
         cTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATcc                                                                                                                                         <  1:1090584/150‑1 (MQ=255)
         cTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATcc                                                                                                                                         <  1:515036/150‑1 (MQ=255)
          tGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCg                                                                                                                                        <  2:599005/150‑1 (MQ=255)
          gggACGCGCGTAACGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTTACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTACCGGATGCGAAAACTCGCATCCg                                                                                                                                        <  2:913607/149‑1 (MQ=255)
           ggATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCgg                                                                                                                                       <  1:1076346/150‑1 (MQ=255)
            gATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGGCTTAACGAGATGGGGAGCGGGCTGGCAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGt                                                                                                                                      <  1:173748/150‑1 (MQ=255)
                cccGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATc                                                                                                                                  >  1:983050/1‑150 (MQ=255)
                  cGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGt                                                                                                                                >  1:986605/1‑150 (MQ=255)
                    tAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCt                                                                                                                              >  1:697449/1‑150 (MQ=255)
                    tAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCt                                                                                                                              >  1:416779/1‑150 (MQ=255)
                       aGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTCACGAGATGGGGATCGTGCTGGAAGATGGCCCTCAAGGGACCACCTGGCGTCGTAAGTACTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcg                                                                                                                           <  2:1526474/150‑1 (MQ=255)
                       aGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcg                                                                                                                           <  1:1018556/150‑1 (MQ=255)
                        gCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACTAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGGAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcgc                                                                                                                          >  2:849727/1‑150 (MQ=255)
                        gCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGGGCTCGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcgc                                                                                                                          <  2:1579412/150‑1 (MQ=255)
                         cGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcc                                                                                                                         <  1:1703549/150‑1 (MQ=255)
                         cGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcc                                                                                                                         <  2:14808/150‑1 (MQ=255)
                           aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                       >  2:1513543/1‑150 (MQ=255)
                            aaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacc                                                                                                                      <  2:1017698/150‑1 (MQ=255)
                             aGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacca                                                                                                                     >  2:605425/1‑150 (MQ=255)
                               aCTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACcaca                                                                                                                   >  2:208814/1‑150 (MQ=255)
                                     cggcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTc                                                                                                             <  1:107555/150‑1 (MQ=255)
                                             gATGCTGCGCGTGATCGTCTAAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGt                                                                                                     <  1:316400/150‑1 (MQ=255)
                                                 cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                 <  1:300570/150‑1 (MQ=255)
                                                 cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                 >  2:378593/1‑150 (MQ=255)
                                                 cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCAGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCGATCAGTAGCa                                                                                                 >  2:1119648/1‑150 (MQ=255)
                                                   gcgcGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATc                                                                                               >  1:1118341/1‑150 (MQ=255)
                                                      cgTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATcccc                                                                                            <  2:697449/150‑1 (MQ=255)
                                                      cgTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATcccc                                                                                            <  2:416779/150‑1 (MQ=255)
                                                         gATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGc                                                                                         >  2:333260/1‑150 (MQ=255)
                                                            cGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAc                                                                                      >  1:1312163/1‑150 (MQ=255)
                                                             gTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAcc                                                                                     >  2:150241/1‑150 (MQ=255)
                                                             gTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAcc                                                                                     >  2:443305/1‑150 (MQ=255)
                                                                 tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                 >  2:453566/1‑150 (MQ=255)
                                                                 tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                 >  1:766167/1‑150 (MQ=255)
                                                                   aCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGCCTGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAccc                                                                               <  2:127906/150‑1 (MQ=255)
                                                                   aCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAccc                                                                               >  2:1203562/1‑150 (MQ=255)
                                                                    cGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCca                                                                              <  2:549048/150‑1 (MQ=255)
                                                                      agaTGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCcaca                                                                            >  2:988458/1‑150 (MQ=255)
                                                                        aTGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATc                                                                          <  1:590266/150‑1 (MQ=255)
                                                                         tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCCCCTCa                                                                         >  2:802307/1‑150 (MQ=255)
                                                                            ggATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAtt                                                                      >  2:13601/1‑150 (MQ=255)
                                                                              aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                    <  2:476458/150‑1 (MQ=255)
                                                                               tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACTAGATTCCCATCAGTAGCATCCCCGGCAACCCCACCCACATCAATTCAg                                                                   >  1:70520/1‑150 (MQ=255)
                                                                               tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                   <  1:1293273/150‑1 (MQ=255)
                                                                                  tGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGaaa                                                                <  1:658342/150‑1 (MQ=255)
                                                                                   gCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTTATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                               >  1:248503/1‑150 (MQ=255)
                                                                                    cTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaata                                                              <  1:1561381/150‑1 (MQ=255)
                                                                                      ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataat                                                            >  1:1553501/1‑150 (MQ=255)
                                                                                      ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataat                                                            >  1:1181294/1‑150 (MQ=255)
                                                                                       gAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCCACCACACCCACATCAATTCAGAAATAATc                                                           >  2:1433416/1‑150 (MQ=255)
                                                                                        aaGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCa                                                          <  1:681214/150‑1 (MQ=255)
                                                                                        aaGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCa                                                          <  1:304070/150‑1 (MQ=255)
                                                                                           aTGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCt                                                       >  1:708979/1‑150 (MQ=255)
                                                                                               cccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATg                                                   <  2:1553501/150‑1 (MQ=255)
                                                                                               cccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATg                                                   >  2:706699/1‑150 (MQ=255)
                                                                                                ccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                  <  1:128411/150‑1 (MQ=255)
                                                                                                   cAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCg                                               <  1:457418/150‑1 (MQ=255)
                                                                                                   aaaGGGACCACATGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCAACACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCg                                               <  1:1134227/149‑1 (MQ=255)
                                                                                                     aGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTa                                             >  2:1380165/1‑150 (MQ=255)
                                                                                                         accaccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACAGCAAGTCAGAAATAATCACCTGATGCCCGTACGGc                                         >  2:169851/1‑150 (MQ=255)
                                                                                                             ccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtgg                                     >  1:235482/1‑150 (MQ=255)
                                                                                                             ccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtgg                                     <  2:983050/150‑1 (MQ=255)
                                                                                                             ccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtgg                                     <  1:849727/150‑1 (MQ=255)
                                                                                                                 gCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACTCCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTa                                 <  1:1062636/150‑1 (MQ=255)
                                                                                                                   gtcgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCAGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAAc                               >  2:1521191/1‑150 (MQ=255)
                                                                                                                    tcgtAAGTAATTGCGCTATTGCCGGATGTGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACg                              <  1:4019/150‑1 (MQ=255)
                                                                                                                     cgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCCGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACga                             <  2:1131338/150‑1 (MQ=255)
                                                                                                                     cgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGGTAGGCGTGGTGTAACga                             >  2:1159636/1‑150 (MQ=255)
                                                                                                                     cgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACga                             >  2:940429/1‑150 (MQ=255)
                                                                                                                      gtaagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgag                            <  2:650548/150‑1 (MQ=255)
                                                                                                                       taagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgaga                           <  1:1203562/150‑1 (MQ=255)
                                                                                                                          gtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACTTGATGCCCGTACGGCGTGGTGTAACGAGACaa                        <  1:605425/150‑1 (MQ=255)
                                                                                                                            aaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATg                      >  2:77476/1‑150 (MQ=255)
                                                                                                                            aaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACaagg                      >  1:640626/1‑148 (MQ=255)
                                                                                                                             aTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGc                     <  2:343340/150‑1 (MQ=255)
                                                                                                                             aTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGc                     >  1:735407/1‑150 (MQ=255)
                                                                                                                             aTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGc                     <  2:338246/150‑1 (MQ=255)
                                                                                                                             aTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGc                     <  2:230711/150‑1 (MQ=255)
                                                                                                                                gcgcTATTGCCGGATGCTAGTTTTCTCATACGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGAATCCCCGGCAACCCCACCCCCATCAATTCAGAAAAAATCAACTGATGCACGTACGTCTTGGTGTAACGAGACACTGCaaa                  >  2:255811/1‑150 (MQ=255)
                                                                                                                                 cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAAc                 <  1:333260/150‑1 (MQ=255)
                                                                                                                                  gcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCATACGGCGTGGTGTAACGAGACAATGCAAACg                >  1:1240809/1‑150 (MQ=255)
                                                                                                                                   cTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCACATCAGTACCATACCCAGCAACCAAACCCACATCAATTCAGAAATAATCACCTGATGACCGTACCGCGTGGTGTAACGAGACAAGGCAAACgg               >  1:290047/1‑150 (MQ=255)
                                                                                                                                     ataaccGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAAACACCTGATGCCCGTACGGCGTGGTGTAACGAGACAAAGCAACCGgcg             >  1:596342/5‑150 (MQ=255)
                                                                                                                                     aTTGCCGGATGCGAGTTTTCGCATCCGGTTATCGGCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAAAACAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAAAGgcg             >  1:776703/1‑150 (MQ=255)
                                                                                                                                       tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcg           <  1:13601/150‑1 (MQ=255)
                                                                                                                                       tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcg           <  1:453566/150‑1 (MQ=255)
                                                                                                                                       tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcg           <  1:443305/150‑1 (MQ=255)
                                                                                                                                        gCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCCCACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcgc          >  2:1115261/1‑149 (MQ=255)
                                                                                                                                        accGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCCCACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGTTGTAACGAGGCAATGCAAACGGGGCGa          >  2:260367/2‑150 (MQ=255)
                                                                                                                                         ccGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCAATCAGTCCCCTCCCCCGCACCAACAAACACATCAATTCAGAAATAATCACCTTATGACCTTACGGCGAGGTGTAACGAGACAATGCACACTGCTCGAc         >  2:288304/1‑150 (MQ=255)
                                                                                                                                          cGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACt        >  1:707714/1‑150 (MQ=255)
                                                                                                                                             aTGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtttt     <  2:1181294/150‑1 (MQ=255)
                                                                                                                                              tGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGGGGTGTAACGAGACAATGCAAACGGCGCGACttttt    >  2:200823/1‑149 (MQ=255)
                                                                                                                                                cgcgatttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTACCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACTGCGTGGTGTAACGAGACAATGAAAACGGCGCGACTTTTATc  <  2:83305/145‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATC  >  NC_000913/555871‑556164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: