Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 568,685 T→A 6.8% intergenic (+38/‑217) emrE → / → ybcK DLP12 prophage; multidrug resistance protein/DLP12 prophage; putative recombinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913568,6850TA 6.8% 348.9 / 9.9 118intergenic (+38/‑217)emrE/ybcKDLP12 prophage; multidrug resistance protein/DLP12 prophage; putative recombinase
Reads supporting (aligned to +/- strand):  ref base T (62/48);  new base A (2/6);  total (64/54)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 133 total aligned reads displayed.

CATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTAA  >  NC_000913/568538‑568833
                                                                                                                                                   |                                                                                                                                                    
cATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCtta                                                                                                                                                    >  2:1581056/1‑150 (MQ=255)
  tGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttata                                                                                                                                                  <  2:805061/150‑1 (MQ=255)
      gATTTTGCGGCCAACGGCTGGACCAGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatattta                                                                                                                                              <  1:1151135/150‑1 (MQ=255)
       aTTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatatttgt                                                                                                                                             >  1:1288192/1‑148 (MQ=255)
        tttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatatttata                                                                                                                                            <  2:662107/150‑1 (MQ=255)
         ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatatttatat                                                                                                                                           >  2:1439935/1‑150 (MQ=255)
         ttttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCttatatttatat                                                                                                                                           <  2:405888/150‑1 (MQ=255)
          tttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATg                                                                                                                                          <  1:1575296/150‑1 (MQ=255)
           ttCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGa                                                                                                                                         <  1:145188/150‑1 (MQ=255)
              ggCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGcc                                                                                                                                      >  1:1277197/1‑150 (MQ=255)
              ggCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTAGATTATTTAATTATTGTCACGAAGCACACCAAAATAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATTATTATATGAGcc                                                                                                                                      >  1:831455/1‑150 (MQ=255)
               gCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCt                                                                                                                                     <  1:845326/150‑1 (MQ=255)
                ccAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTTATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATTATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTc                                                                                                                                    <  2:476245/150‑1 (MQ=255)
                ccAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTc                                                                                                                                    >  1:1368183/1‑150 (MQ=255)
                ccAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTc                                                                                                                                    <  1:525453/150‑1 (MQ=255)
                ccAACGGCTGGACCTGACAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTc                                                                                                                                    <  2:1577473/150‑1 (MQ=255)
                   aCGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATAAATATAAGAGCCTccat                                                                                                                                 <  2:1691870/150‑4 (MQ=255)
                    cGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGttt                                                                                                                                >  1:1691825/1‑150 (MQ=255)
                    cGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGttt                                                                                                                                <  1:1268934/150‑1 (MQ=255)
                     ggCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGtttt                                                                                                                               >  2:1232572/1‑150 (MQ=255)
                     ggCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGtttt                                                                                                                               >  1:140504/1‑150 (MQ=255)
                        tGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATg                                                                                                                            <  1:472778/150‑1 (MQ=255)
                         tgACCTGCAATACATTATAGGTATTATGTTGATTTGTGCATGTGTGTTGATTATTAATTTATTTTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGc                                                                                                                           <  1:229481/149‑1 (MQ=255)
                         ggcaggtccagccgTTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGc                                                                                                                           >  2:135851/8‑150 (MQ=255)
                            ccTTCCAGCCATTATAGGCATGATTTTGATTTGTTCCTGTGTGTTGAGTATTAATTTATTGTCACGAAGCACACCACATTAAAATTATTTGTTTCTAAACGACTAAATTATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCttt                                                                                                                        <  1:467337/150‑1 (MQ=255)
                             cTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCtttt                                                                                                                       >  2:936510/1‑150 (MQ=255)
                                 cagccaTTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGtt                                                                                                                   >  1:1498198/1‑150 (MQ=255)
                                 cagccaTTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGtt                                                                                                                   >  2:281149/1‑150 (MQ=255)
                                 cagccaTTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCAAATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCtttttttt                                                                                                                   >  2:1405075/1‑150 (MQ=255)
                                       ttATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTc                                                                                                             <  1:1214648/150‑1 (MQ=255)
                                         ataGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCtt                                                                                                           <  1:965952/150‑1 (MQ=255)
                                         ataGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCtt                                                                                                           <  1:630410/150‑1 (MQ=255)
                                          taGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCttt                                                                                                          <  1:1633112/150‑1 (MQ=255)
                                                tgatgTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATTAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAttttt                                                                                                    >  1:769161/1‑150 (MQ=255)
                                                tgatgTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTAttttt                                                                                                    >  1:762633/1‑150 (MQ=255)
                                                  atgTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTa                                                                                                  <  2:105451/150‑1 (MQ=255)
                                                    gTTTATTTGTGCCTGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGAATAAAATATGGAGGCTATTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATg                                                                                                <  1:474498/150‑1 (MQ=255)
                                                     ttGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACTACTAAAATATTGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGt                                                                                               >  1:267221/1‑150 (MQ=255)
                                                              tcaggTGTTTTTATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtg                                                                                      <  1:1605376/147‑1 (MQ=255)
                                                              gCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtg                                                                                      <  2:172517/150‑1 (MQ=255)
                                                              gCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtg                                                                                      <  1:1156252/150‑1 (MQ=255)
                                                              cccGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTAGTATCTAAACGACTAAAATATGCAGGCTCTTAAATATATATTAGCCTCGTTTTATGCTTTTAGTTAATGTATTTATTTTTTATGTattattatt                                                                                      >  1:139685/2‑147 (MQ=255)
                                                               ccGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTGTATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtgt                                                                                     <  2:1288192/150‑1 (MQ=255)
                                                                cGGTGTGTTGATTTTTAATTTATTGTCTCGTAGCACACCACATTAAAATAATTTGTTTCTAAACGACTATAATATGGAGGCTCTTATTTTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTtgtg                                                                                    <  1:1110757/150‑1 (MQ=255)
                                                                   tgtgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCtt                                                                                 >  1:1477183/1‑150 (MQ=255)
                                                                     tgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCttta                                                                               >  1:1414135/1‑149 (MQ=255)
                                                                     tgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCttta                                                                               >  2:364950/1‑149 (MQ=255)
                                                                     tgtTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTc                                                                               >  1:922950/1‑150 (MQ=255)
                                                                       ttGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTAAGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTaaa                                                                             >  1:156751/1‑150 (MQ=255)
                                                                        tGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAg                                                                            >  2:608225/1‑150 (MQ=255)
                                                                         gATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGATTTCAAGa                                                                           >  1:361435/1‑150 (MQ=255)
                                                                               taATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCATATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATgg                                                                     <  2:334898/150‑1 (MQ=255)
                                                                                    tATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTaa                                                                >  2:1631214/1‑150 (MQ=255)
                                                                                     aTTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAg                                                               <  1:533405/150‑1 (MQ=255)
                                                                                     aTTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAg                                                               <  2:1368647/150‑1 (MQ=255)
                                                                                      ttGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCATGATTATGGCGTAAGa                                                              >  1:401586/1‑150 (MQ=255)
                                                                                            cGAAGCACACCACATTAAAATAATTTGTTTCTAATCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTg                                                        >  2:1515666/1‑150 (MQ=255)
                                                                                            cGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTg                                                        >  1:812204/1‑150 (MQ=255)
                                                                                                gCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAAt                                                    >  1:1315652/1‑150 (MQ=255)
                                                                                                 cacaCCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATa                                                   <  1:659323/150‑1 (MQ=255)
                                                                                                 cacaCCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATa                                                   >  1:1699713/1‑150 (MQ=255)
                                                                                                   caccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAg                                                               >  1:1219565/1‑136 (MQ=255)
                                                                                                   caccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAg                                                               <  2:1219565/136‑1 (MQ=255)
                                                                                                   caccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACg                                                 <  1:567071/150‑1 (MQ=255)
                                                                                                   caccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACg                                                 <  2:1295681/150‑1 (MQ=255)
                                                                                                     ccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGat                                               >  2:1557692/1‑150 (MQ=255)
                                                                                                     ccacATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGat                                               >  2:961273/1‑150 (MQ=255)
                                                                                                       acaTTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTTTATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGatta                                             >  1:502229/1‑150 (MQ=255)
                                                                                                          ttAAATTAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGattattt                                          >  2:1155882/1‑149 (MQ=255)
                                                                                                           tAAAATAATTTGTTTCTTAACGACTAAAATATGGAGTGTCTTATATTTATATGTGGCTCGTTTTTTTCTTTTTGTTAATTTCATTATTTTTTATGTATTCTTTTGTGCTTTAAAGATTATGGCGTAAGAAAATTGCAATACGATAAttgt                                         <  2:831455/150‑1 (MQ=255)
                                                                                                           tAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTAttgt                                         <  2:1368183/150‑1 (MQ=255)
                                                                                                           tAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGACTTAATTTTTTATTTATTATTTTGTGATTTCAAGATTATGACGTAAGAAAATTGAAATACTattatttt                                         >  1:1065383/1‑148 (MQ=255)
                                                                                                               ataatTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGTCTCGTTTTATGCATTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGta                                     <  2:1181754/150‑1 (MQ=255)
                                                                                                               ataatTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTAGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGta                                     <  1:201413/150‑1 (MQ=255)
                                                                                                                       tttCTTAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAg                             <  2:321902/150‑1 (MQ=255)
                                                                                                                       tttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCaat                             >  1:399991/1‑149 (MQ=255)
                                                                                                                       tttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAg                             <  1:135851/150‑1 (MQ=255)
                                                                                                                        ttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGa                            <  2:380261/150‑1 (MQ=255)
                                                                                                                        ttCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGa                            >  1:306581/1‑150 (MQ=255)
                                                                                                                           tAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATaa                         >  1:520058/1‑150 (MQ=255)
                                                                                                                            aaaCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAAt                        >  1:346943/1‑150 (MQ=255)
                                                                                                                              aCGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAAtgt                      >  1:460303/1‑150 (MQ=255)
                                                                                                                                gACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGa                    >  2:1435671/1‑150 (MQ=255)
                                                                                                                                  cTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGAcc                  <  2:600359/150‑1 (MQ=255)
                                                                                                                                    aaaaTATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCtt                >  1:566724/1‑150 (MQ=255)
                                                                                                                                         atGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTg           <  2:1315652/150‑1 (MQ=255)
                                                                                                                                         atGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTg           >  1:651884/1‑150 (MQ=255)
                                                                                                                                         aacgAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTg           >  1:674214/4‑150 (MQ=255)
                                                                                                                                           ggAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCttt                                                                                        <  1:720297/71‑1 (MQ=255)
                                                                                                                                           ggAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCttt                                                                                        >  2:720297/1‑71 (MQ=255)
                                                                                                                                            gAGGCTCATATAAATATAAGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCt        >  2:719008/1‑150 (MQ=255)
                                                                                                                                             aGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCtt       >  1:1231731/1‑150 (MQ=255)
                                                                                                                                              ggCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCATGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttt      <  1:424285/150‑1 (MQ=255)
                                                                                                                                              ggCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttt      >  2:576089/1‑150 (MQ=255)
                                                                                                                                              ggCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttt      <  1:936510/150‑1 (MQ=255)
                                                                                                                                              ggCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttt      >  2:1679570/1‑150 (MQ=255)
                                                                                                                                               gCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCtttt     <  2:1477183/150‑1 (MQ=255)
                                                                                                                                                ctctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttttt    >  2:440957/1‑150 (MQ=255)
                                                                                                                                                ctctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCttttt    >  2:1140849/1‑150 (MQ=255)
                                                                                                                                                ctctTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTAAATTGTCttttt    >  1:484526/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CATGGGGATTTTTCGGCCAACGGCTGGACCTGCCAGCCATTATAGGCATGATGTTGATTTGTGCCGGTGTGTTGATTATTAATTTATTGTCACGAAGCACACCACATTAAAATAATTTGTTTCTAAACGACTAAAATATGGAGGCTCTTATATTTATATGAGCCTCGTTTTATGCTTTTTGTTAATGTCTTTATTTTTTATGTATTCTTTTGTGCTTTCAAGATTATGGCGTAAGAAAATTGCAATACGATTATTGTTGTATATTCAAGATAATGTGACCTTAATTGTCTTTTTAA  >  NC_000913/568538‑568833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: