Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 605,022 C→G 6.0% D135H (GAT→CAT)  nfsB ← dihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913605,0220CG 6.0% 382.3 / 12.3 117D135H (GAT→CAT) nfsBdihydropteridine reductase, NAD(P)H‑dependent, oxygen‑insensitive
Reads supporting (aligned to +/- strand):  ref base C (51/59);  new base G (2/5);  total (53/64)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 132 total aligned reads displayed.

GGTGTAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCA  >  NC_000913/604873‑605170
                                                                                                                                                     |                                                                                                                                                    
ggTGTAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAACCCTGTTTTGCCATCCACTCTGcatc                                                                                                                                                      >  2:584732/1‑150 (MQ=255)
ggTGTAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGAACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatc                                                                                                                                                      >  1:319027/1‑150 (MQ=255)
  tgtAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCTATGGGTACAGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAACCCTGTTTTGCCATCCACTCTGcatcat                                                                                                                                                    >  1:285396/1‑150 (MQ=255)
  tgtAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcat                                                                                                                                                    >  1:369767/1‑150 (MQ=255)
   gtAGCCTTTCTCTTTCAGACCAAATTCTGCGTCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcatc                                                                                                                                                   >  2:336144/1‑150 (MQ=255)
   gtAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcatc                                                                                                                                                   <  1:22548/150‑1 (MQ=255)
    tAGCCTTTCTCTTTCATACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCTTCCAGACCCAGAGCCACCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcatca                                                                                                                                                  <  1:938534/150‑1 (MQ=255)
    tAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcatca                                                                                                                                                  <  2:13666/150‑1 (MQ=255)
    tAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGcatcatca                                                                                                                                                  <  1:465578/150‑1 (MQ=255)
        cTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCACCCACTCTGCATCATCATGCa                                                                                                                                              >  1:629173/1‑150 (MQ=255)
         tttCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAg                                                                                                                                             <  1:90842/150‑1 (MQ=255)
         tttCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAg                                                                                                                                             <  1:489980/150‑1 (MQ=255)
          ttCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGa                                                                                                                                            <  1:134270/150‑1 (MQ=255)
           tctcTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGAt                                                                                                                                           >  1:1206005/1‑150 (MQ=255)
            gtctTTCAGACCTATTTATGCATGGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCGAGAGCCGCCACGCCGAGCTGGATGTTACCGACGTTGAGATAAACCTGTTTTGCCATGCACTCTGCATGATGATGCAGagt                                                                                                                                          <  2:704510/149‑3 (MQ=255)
            ctctTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGATGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                                                                          <  2:1448521/150‑3 (MQ=255)
            ctctTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                                                                          <  2:557593/150‑3 (MQ=255)
            ctctTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATc                                                                                                                                          <  1:477067/150‑1 (MQ=255)
            ctctTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATAGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagt                                                                                                                                          <  2:1326035/150‑3 (MQ=255)
             tctTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATGATGATGCAGagtg                                                                                                                                         <  2:1544447/150‑4 (MQ=255)
             tctTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCt                                                                                                                                         >  1:495050/1‑150 (MQ=255)
              ctTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCtt                                                                                                                                        >  2:650821/1‑150 (MQ=255)
               tttCAGCCCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGACGAGCAGGAAGTTACCGACGTTGACATAAACCTGTTTTTACATCCACTCTGCATCATCATCCAGATCttt                                                                                                                                       >  2:942715/1‑150 (MQ=255)
                 tCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTAc                                                                                                                                     <  1:285869/150‑1 (MQ=255)
                   aGACCAAATTCTGCATCGGGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACgg                                                                                                                                   <  2:1174978/150‑1 (MQ=255)
                   aGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACgg                                                                                                                                   <  1:208285/150‑1 (MQ=255)
                   aGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTATTGCCATCCACTCTGCATCATCATGCAGATCTTTACgg                                                                                                                                   >  1:708475/1‑150 (MQ=255)
                    gACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGt                                                                                                                                  >  2:1579611/1‑150 (MQ=255)
                      ccAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGc                                                                                                                                >  1:1659942/1‑150 (MQ=255)
                      ccAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGc                                                                                                                                <  2:1698899/150‑1 (MQ=255)
                        acttgcTGCAGCGAGGATGGCCGCGGCAAAACCTTCGAGGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCat                                                                                                                              <  2:264535/145‑1 (MQ=255)
                        aatttCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCTTGCAGATCTTTACGGTGCat                                                                                                                              <  2:754041/147‑1 (MQ=255)
                          aTTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCatat                                                                                                                            >  1:802658/1‑150 (MQ=255)
                             cTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAg                                                                                                                         >  2:133046/1‑150 (MQ=255)
                             aagaaTCGTGGATGGCGGCGTGAAAACCTTCGATGGGTACAGCGGCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTTAGATATACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAg                                                                                                                         <  2:1445668/146‑1 (MQ=255)
                               gCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTCAGATAAACATGTTTTGCCATCCACTCAGCATCATCATGCAGATCTTTACGGTGCATATCAGCg                                                                                                                       >  2:1665458/1‑150 (MQ=255)
                                  tCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCgaag                                                                                                                    <  1:336144/150‑1 (MQ=255)
                                     tggttggtggCGTCAAAACCTTCGATTTGTACCGCGTCCAGAACCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGAATATCAGCGAAGAAc                                                                                                                 <  2:1118191/146‑1 (MQ=255)
                                        ttGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTg                                                                                                              <  2:285396/149‑1 (MQ=255)
                                        aTGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTg                                                                                                              <  2:369767/150‑1 (MQ=255)
                                             ggcgTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGAcc                                                                                                         >  2:1455486/1‑150 (MQ=255)
                                             ggcgTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGAcc                                                                                                         >  1:33571/1‑150 (MQ=255)
                                                 tCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTGTACGGTGCATATCAGCGAAGAACTTGCGACCTTTa                                                                                                     >  1:1205497/1‑150 (MQ=255)
                                                  cAATACCTTCGATGGGTACCGCGTCCAGACGCAGAGCCGCCACGCCGAGCAGGTAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTAt                                                                                                    <  1:600593/150‑1 (MQ=255)
                                                     aaCCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGt                                                                                                 >  2:827288/1‑150 (MQ=255)
                                                        cTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTcg                                                                                              >  2:1370667/1‑150 (MQ=255)
                                                                  tACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGCCCTTTATCGTTCGCGGCTTTctc                                                                                    >  1:1100675/1‑148 (MQ=255)
                                                                     cgcgTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTc                                                                                 >  1:879960/1‑150 (MQ=255)
                                                                       cgTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCg                                                                               >  2:344092/1‑150 (MQ=255)
                                                                       cgTACAGACCCAGAGCCGCCTCGCCGAGCAGTAAGTTACCTACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTTCGTCCTTTATCGTTCGCGGCTTTCGCTTCCg                                                                               <  1:942715/150‑1 (MQ=255)
                                                                        gTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCgg                                                                              <  1:1241531/150‑1 (MQ=255)
                                                                         tCCATACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGc                                                                             <  2:1269602/150‑1 (MQ=255)
                                                                          ccAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCg                                                                            >  1:900896/1‑150 (MQ=255)
                                                                            aGACCCAGAGCCGCCACGCCGAGCACTAAGTAACCGACGTTGAGATATACCTGTTTAGCCATCCACTCTGCATCATCATGCAGATCTTTACGGAGCATATCAGCGAACAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTg                                                                          <  1:284724/150‑1 (MQ=255)
                                                                              aCCCAGAGCCTCCACGCCGAGCAGGTAGTAACCGACGTTGTGATAAACCTGTTTTGCCTTCCACTCTGCATCATCATGCAGATGTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGc                                                                        <  2:1701078/150‑1 (MQ=255)
                                                                              aCCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGc                                                                        <  2:1648075/150‑1 (MQ=255)
                                                                               cccAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCa                                                                       >  1:1104638/1‑150 (MQ=255)
                                                                               cccAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCa                                                                       >  1:500146/1‑150 (MQ=255)
                                                                                  gacgccgccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAg                                                                    <  2:1583335/147‑1 (MQ=255)
                                                                                  agagCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGCACTTGCGACCTTTATCGTTAGCGGCTTTCGCTTCCGGCGTGGCAAAg                                                                    >  2:1148381/1‑150 (MQ=255)
                                                                                        gccCCGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGATTCCGGCGTGTCAAAGCGGCCa                                                              >  1:1063021/1‑150 (MQ=255)
                                                                                        gccACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCAGCATGCAGAGCTTTACGGTGCTTATCAGCGAAGACCTTGCGACCGTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCa                                                              >  1:1063016/1‑150 (MQ=255)
                                                                                              ccGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCa                                                        >  1:127585/1‑150 (MQ=255)
                                                                                                   cAGGAAGTTACCGCCGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTc                                                   >  1:1070352/1‑150 (MQ=255)
                                                                                                    aGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTcc                                                  >  2:1244603/1‑150 (MQ=255)
                                                                                                           tACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTCCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCTGCTTTCGATTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTcaa                                           >  2:409632/1‑150 (MQ=255)
                                                                                                             ccGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTcaaca                                         <  1:1294266/150‑1 (MQ=255)
                                                                                                             ccGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTcaaca                                         <  2:1206005/150‑1 (MQ=255)
                                                                                                               gACGTTTAGATAACACTGTTTTGATATCCACTCTGCATCATCATGCAGATCTTTAAGGTGAATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCaacaac                                       <  2:1036815/150‑1 (MQ=255)
                                                                                                               gACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCTGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTGTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCaacaac                                       <  2:1036817/150‑1 (MQ=255)
                                                                                                                aCGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGATCTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAAcc                                      <  1:133046/150‑1 (MQ=255)
                                                                                                                 cGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCTACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCa                                     <  1:584732/150‑1 (MQ=255)
                                                                                                                 cGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCa                                     <  1:1685330/150‑1 (MQ=255)
                                                                                                                 cGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGATCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCa                                     <  2:319027/150‑1 (MQ=255)
                                                                                                                  gTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAg                                    <  2:802658/150‑1 (MQ=255)
                                                                                                                  gTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAg                                    >  1:1637790/1‑150 (MQ=255)
                                                                                                                  gTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAg                                    <  1:524465/150‑1 (MQ=255)
                                                                                                                  gTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAg                                    <  1:517887/150‑1 (MQ=255)
                                                                                                                    tGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCt                                  <  1:1579611/150‑1 (MQ=255)
                                                                                                                      agaTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTc                                <  2:879960/150‑1 (MQ=255)
                                                                                                                      agaTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTc                                >  2:583809/1‑150 (MQ=255)
                                                                                                                      agaTAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTc                                <  2:495050/150‑1 (MQ=255)
                                                                                                                           aaCCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCa                           >  1:1581655/1‑150 (MQ=255)
                                                                                                                            aCCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAg                          >  1:1353604/1‑150 (MQ=255)
                                                                                                                               tGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCACCCACCAGCTTCAGCCAGACa                       >  1:1059056/1‑150 (MQ=255)
                                                                                                                                     gCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTcc                 <  1:1455486/150‑1 (MQ=255)
                                                                                                                                     gCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTcc                 >  1:1120283/1‑150 (MQ=255)
                                                                                                                                      ccATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCTAAGCGGCCATCGGCATCTTCCTGGTCAACACCCCGCTGCCGCCAGCCATCGTCCa                >  2:129737/1‑150 (MQ=255)
                                                                                                                                      ccATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCa                <  2:500146/150‑1 (MQ=255)
                                                                                                                                      ccATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCa                >  1:1391497/1‑150 (MQ=255)
                                                                                                                                       gtaaagatctGCATGTTGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCAt               >  2:198777/8‑150 (MQ=255)
                                                                                                                                       gtaaagatctGCATGTTGATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCAt               >  2:192939/8‑150 (MQ=255)
                                                                                                                                       cATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCaa               >  2:1391884/1‑149 (MQ=255)
                                                                                                                                           cACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATcgcg           >  2:1533689/1‑150 (MQ=255)
                                                                                                                                            aCTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCgg          <  1:760182/150‑1 (MQ=255)
                                                                                                                                             ctctGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGt         >  2:667478/1‑150 (MQ=255)
                                                                                                                                                tGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGtttt      >  1:517337/1‑150 (MQ=255)
                                                                                                                                                  catcatcatGCAGATCTTTACGGTGCATATCAGCGATGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTg    <  1:1665458/150‑1 (MQ=255)
                                                                                                                                                  catcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTg    >  1:498039/1‑150 (MQ=255)
                                                                                                                                                   atcatcatGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGc   <  2:900896/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGTGTAGCCTTTCTCTTTCAGACCAAATTCTGCATCGAGGATGGCGGCGTCAAAACCTTCGATGGGTACCGCGTCCAGACCCAGAGCCGCCACGCCGAGCAGGAAGTTACCGACGTTGAGATAAACCTGTTTTGCCATCCACTCTGCATCATCATGCAGATCTTTACGGTGCATATCAGCGAAGAACTTGCGACCTTTATCGTTCGCGGCTTTCGCTTCCGGCGTGGCAAAGCGGCCATCGGCATCTTCCTGGTCAACAACCAGCTTCAGCCAGACATCGTCCATCGCGGTTTTTGCA  >  NC_000913/604873‑605170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: