Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 622,455 G→C 6.3% W52C (TGG→TGC entS → enterobactin exporter, iron‑regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913622,4550GC 6.3% 265.3 / 7.0 80W52C (TGG→TGCentSenterobactin exporter, iron‑regulated
Reads supporting (aligned to +/- strand):  ref base G (40/35);  new base C (2/3);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

AACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACTGTGCCTTAA  >  NC_000913/622307‑622604
                                                                                                                                                    |                                                                                                                                                     
aaCAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGTTGCCGGGGCAGAGCCAGATGATGACACATTCCACCTGGc                                                                                                                                                      >  2:1525418/1‑150 (MQ=255)
 aCAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGCCa                                                                                                                                                     >  2:14914/1‑150 (MQ=255)
  cAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAg                                                                                                                                                    >  2:473127/1‑150 (MQ=255)
       cTGGCTGCTTAAGCTCAGACTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGGGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTggg                                                                                                                                               <  1:503631/150‑1 (MQ=255)
          gctgctTAACCTCAGCCTGTTGAAACCGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGCCAGGTggaatg                                                                                                                                            <  1:649399/150‑5 (MQ=255)
          gctgctTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGCCAGGTggaatg                                                                                                                                            <  1:14914/150‑5 (MQ=255)
          gctgctTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGCCAGGTggaatg                                                                                                                                            <  1:848636/150‑5 (MQ=255)
             gctTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTc                                                                                                                                         >  1:286704/1‑150 (MQ=255)
                 aaCCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTg                                                                                                                                     <  2:1269094/150‑1 (MQ=255)
                   ccTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGAc                                                                                                                                   >  1:692860/1‑150 (MQ=255)
                       aGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTg                                                                                                                               <  1:69110/150‑1 (MQ=255)
                       aGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCAGCTCAATGGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTg                                                                                                                               <  1:83779/150‑1 (MQ=255)
                              tGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACcggcg                                                                                                                        >  2:898342/1‑150 (MQ=255)
                               gAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACcggcgg                                                                                                                       >  1:210938/1‑150 (MQ=255)
                               gAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAAATGTGTCTCTGGGTTTGCTCGGCGTCGAGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGAGACGCTGACcggcgg                                                                                                                       >  2:286747/1‑150 (MQ=255)
                                aaaaCGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCggcggc                                                                                                                      <  1:622038/150‑1 (MQ=255)
                                 aaaCGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACcgg                                                                                                                          <  2:1047167/145‑1 (MQ=255)
                                 aaaCGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACcgg                                                                                                                          >  1:1047167/1‑145 (MQ=255)
                                    cGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGa                                                                                                                  >  2:1443325/1‑150 (MQ=255)
                                     gcccccGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGCCTCTGGGTTTGCTCGGCGGCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGAt                                                                                                                 <  2:641280/147‑1 (MQ=255)
                                      cccccGGCGGATCGCGCAGTACTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGGCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGAtg                                                                                                                <  2:1718180/148‑1 (MQ=255)
                                          cGGCGTTTCGCGCCGTATTCCTCGCTCGTTTCCTCTCCATTGTGGCTCTGGGTTTGCTCGGCGGCGCGGTGCCTGTGCTGATCCAGATGATGACACATTCCTCCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGTCGGCGCGAtgtttg                                                                                                            <  1:267140/150‑1 (MQ=255)
                                          cGGAGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGTGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATACAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGAtgtttg                                                                                                            >  2:748875/1‑150 (MQ=255)
                                               tttCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAAATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGc                                                                                                       <  1:1057939/150‑1 (MQ=255)
                                                 tCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCt                                                                                                     <  1:1187248/150‑1 (MQ=255)
                                                   gcgcAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTGTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGa                                                                                                   >  1:117581/1‑150 (MQ=255)
                                                      cAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATgg                                                                                                <  2:317822/150‑1 (MQ=255)
                                                          aTTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGGcgg                                                                                            <  1:964838/150‑1 (MQ=255)
                                                          aTTCCTCGCTCGTTTCATCTCAATTGTGGCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTcgg                                                                                            <  1:1573474/150‑1 (MQ=255)
                                                           ttCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTcggc                                                                                           <  1:1353941/150‑1 (MQ=255)
                                                           ttCCTCGCTCGTTGCATCTCAATTGTGTCTCTGGGGTTGCTCGGCGTCGCGGTGCCGGGGCAGATCCAGATGAGGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTcggc                                                                                           <  1:895918/150‑1 (MQ=255)
                                                               tcgctcgTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGTCTCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGgtg                                                                                       <  1:286747/150‑1 (MQ=255)
                                                                    cgTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTg                                                                                  >  1:770206/1‑150 (MQ=255)
                                                                     gTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTgg                                                                                 >  1:1104775/1‑150 (MQ=255)
                                                                       ttCATCTCCATGGGGGCTCTGGGTGTGCTCGGCGTCGCGGTGCCGTTTCAGAACCAGATGATGCCACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCg                                                                               <  2:1419728/150‑1 (MQ=255)
                                                                       ttCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCg                                                                               <  1:1175454/150‑1 (MQ=255)
                                                                         cATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGa                                                                             >  1:1016381/1‑150 (MQ=255)
                                                                         cATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGa                                                                             >  1:107183/1‑150 (MQ=255)
                                                                            ctcAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGgtg                                                                                       >  2:1576323/1‑137 (MQ=255)
                                                                            ctcAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGgtg                                                                                       <  1:1576323/137‑1 (MQ=255)
                                                                              cAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCt                                                                        >  1:1409282/1‑150 (MQ=255)
                                                                              cAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCt                                                                        >  1:1668370/1‑150 (MQ=255)
                                                                                    ggtCTCTGGGTTTGCGCGGCGGCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAgc                                                                  <  2:692860/149‑1 (MQ=255)
                                                                                     gtCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAgcg                                                                 >  1:946011/1‑150 (MQ=255)
                                                                                     gtCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACACTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAgcg                                                                 <  2:1080664/150‑1 (MQ=255)
                                                                                     gtCGCTGGGGTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGGGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAgcg                                                                 <  1:55583/150‑1 (MQ=255)
                                                                                     ggctctGGGTGTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAgcg                                                                 <  1:784789/148‑1 (MQ=255)
                                                                                       ctctGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCa                                                               >  2:428613/1‑150 (MQ=255)
                                                                                           gggTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCaaaat                                                           >  1:427629/1‑149 (MQ=255)
                                                                                               ttGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTg                                                       >  2:1167276/1‑150 (MQ=255)
                                                                                               ttGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTg                                                       >  1:755153/1‑150 (MQ=255)
                                                                                               tggCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGCTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTg                                                       <  2:295846/148‑1 (MQ=255)
                                                                                                 gCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGAt                                                     >  1:1714155/1‑150 (MQ=255)
                                                                                                 gCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGAt                                                     >  1:406491/1‑150 (MQ=255)
                                                                                                  cTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGAtt                                                    >  1:1339562/1‑150 (MQ=255)
                                                                                                     ggCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTTGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTg                                                 <  1:1436377/150‑1 (MQ=255)
                                                                                                     ggCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTg                                                 >  2:807798/1‑150 (MQ=255)
                                                                                                      gCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGc                                                <  1:473127/150‑1 (MQ=255)
                                                                                                         tCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTgg                                             <  2:651766/150‑1 (MQ=255)
                                                                                                         gcgcgGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTgg                                             <  1:41946/149‑1 (MQ=255)
                                                                                                          cgcgGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGgc                                            <  2:559105/150‑1 (MQ=255)
                                                                                                                 ccggtgcAGATCCAGATGATGACACCTTCCACCTGGCAGGGGGGGCTTTCGGTGACGCTGACCGGCGCCGCTATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGc                                     <  2:344246/150‑1 (MQ=255)
                                                                                                                   ggtgcAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCACAAAAAAGTGATTTTGCTGTCGCGCGGCAc                                   >  2:939563/1‑150 (MQ=255)
                                                                                                                      gcAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCtg                                >  2:524343/1‑150 (MQ=255)
                                                                                                                        aGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCtgtg                              >  1:1677021/1‑150 (MQ=255)
                                                                                                                        aGATCCAGATGATGACACATTCCACCGGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCtgtg                              >  1:461941/1‑150 (MQ=255)
                                                                                                                         gATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTgg                             >  2:879165/1‑150 (MQ=255)
                                                                                                                             cAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCtgtgcccat                         >  1:148311/1‑145 (MQ=255)
                                                                                                                              cgatgatGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTg                        <  1:1298003/149‑1 (MQ=255)
                                                                                                                              aGATGATGCCACAGTCCAGCTGGCAGGTGGGGCTCTCGGTGACGCTGCGCGGCGGCGCGATGTTTGTGGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTg                        <  1:250542/150‑1 (MQ=255)
                                                                                                                              aGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTg                        >  2:676258/1‑150 (MQ=255)
                                                                                                                                atgatgACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGc                      <  2:770206/150‑1 (MQ=255)
                                                                                                                                 tgatgaCACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCt                     >  2:1508504/1‑150 (MQ=255)
                                                                                                                                 tgatgaCACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCt                     >  2:1129743/1‑150 (MQ=255)
                                                                                                                                 tgatgaCACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCt                     <  2:406491/150‑1 (MQ=255)
                                                                                                                                  gatgaCACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCtt                    >  2:738528/1‑150 (MQ=255)
                                                                                                                                   atgaCACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGAATTGGCtta                   >  2:1170638/1‑149 (MQ=255)
                                                                                                                                    tgaCACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCa                  >  2:1066915/1‑150 (MQ=255)
                                                                                                                                          aTTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGAc            >  2:704001/1‑150 (MQ=255)
                                                                                                                                           ccccACCTGCCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTGCATTGGACt           >  2:91359/3‑150 (MQ=255)
                                                                                                                                                   ggCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACTGTGCCTTa   <  2:1182200/150‑1 (MQ=255)
                                                                                                                                                    gCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACTGTGCCTTaa  >  1:1585520/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACAATCCTGGCTGCTTAACCTCAGCCTGTTGAAAACGCACCCGGCGTTTCGCGCAGTATTCCTCGCTCGTTTCATCTCAATTGTGTCTCTGGGTTTGCTCGGCGTCGCGGTGCCGGTGCAGATCCAGATGATGACACATTCCACCTGGCAGGTGGGGCTTTCGGTGACGCTGACCGGCGGCGCGATGTTTGTTGGCCTGATGGTCGGCGGTGTGCTGGCGGATCGCTATGAGCGCAAAAAAGTGATTTTGCTGGCGCGCGGCACCTGTGGCATTGGCTTCATTGGACTGTGCCTTAA  >  NC_000913/622307‑622604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: