Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 632,077 T→C 5.3% intergenic (+78/‑105) cstA → / → ybdD carbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913632,0770TC 5.3% 234.3 / 4.9 95intergenic (+78/‑105)cstA/ybdDcarbon starvation protein involved in peptide utilization; APC peptide transporter family protein/DUF466 family protein
Reads supporting (aligned to +/- strand):  ref base T (35/53);  new base C (2/3);  total (37/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.17e-02

AAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGC  >  NC_000913/631928‑632225
                                                                                                                                                     |                                                                                                                                                    
aaagaaaCGCCGTATGAACCCAGGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCTCTCTAAAGTCAGAGTGAGGGGGGGATGGTGGCGAATGGTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGt                                                                                                                                                      <  1:898801/150‑1 (MQ=255)
aaagaaaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGt                                                                                                                                                      <  2:473433/150‑1 (MQ=255)
     aaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGc                                                                                                                                                 <  2:484905/150‑1 (MQ=255)
     aaCGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGAGCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGc                                                                                                                                                 <  1:520371/150‑1 (MQ=255)
     aaCGCCGTATGAACCAATGCCGGAAAATGTCGACGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAACTCAGAGTGAGGGGGGGATGGTGGCGAATGTTGGCTTAGTGCCCAGGGGTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGc                                                                                                                                                 <  1:432911/150‑1 (MQ=255)
          cGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCGCCCCACCGGGGAGAggg                                                                                                                                            <  2:847850/150‑1 (MQ=255)
          cGTATGAACCAATGCCGGAAAAGGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGGCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCGCCCCACCGGGGAGAggg                                                                                                                                            <  1:1294217/150‑1 (MQ=255)
           gTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAgggg                                                                                                                                           <  2:376834/150‑1 (MQ=255)
              tGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCGCCCCACCGGGGAGAgggttag                                                                                                                                        <  1:457727/150‑5 (MQ=255)
               gAACCAATGCCGGAAAATGGCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTCGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTg                                                                                                                                       <  2:371545/150‑1 (MQ=255)
                aaCCAATGCCGGAAAATTTATAGGAGATCGTGGCGCAGGCAAAAGTCGCACACGACAGTCAGAGTGATGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGAGACGGGACTGa                                                                                                                                      >  2:1178776/1‑150 (MQ=255)
                aaCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGa                                                                                                                                      <  2:1343774/150‑1 (MQ=255)
                 aCCAATGCCGGAAATTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAc                                                                                                                                     <  1:674285/150‑1 (MQ=255)
                 aCCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAc                                                                                                                                     <  1:720439/150‑1 (MQ=255)
                  ccAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGAcc                                                                                                                                    <  1:912393/150‑1 (MQ=255)
                       gCCGGAAAATGTCGAGGAGATCGGGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGGGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAgcg                                                                                                                               <  2:286464/150‑1 (MQ=255)
                        ccGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGCCCGAgcgc                                                                                                                              >  2:856642/1‑150 (MQ=255)
                           gataaTGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACGCTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCACTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGCGACTGACCGAGCGCGtt                                                                                                                           >  2:319151/4‑150 (MQ=255)
                           gAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTCGGCGAATGTTGGCTTAGTGCCGAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGtt                                                                                                                           <  2:213237/150‑1 (MQ=255)
                              aaTGGCGAGTAGATCGTGGCGCAGGCAAAAGGCGCACACTTAAGTCCGAGTGAGGGGTGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAt                                                                                                                        <  2:310586/150‑1 (MQ=39)
                                tggcGAGGAGCGCGTGGCGCAGGCAACAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGGTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAg                                                                                                                      <  1:679258/147‑1 (MQ=39)
                                tGTCGAGGAGATCGTGGCGCAGGCTAAAGGCGCACACTAAAGTCCGAGTGTGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAg                                                                                                                      <  1:828195/150‑1 (MQ=255)
                                  tCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCa                                                                                                                    <  1:636053/150‑1 (MQ=255)
                                  gcGAGGAGAGCGTGGCGCAGGCAAAAGGCGCACACTAAAGGCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCa                                                                                                                    <  1:412041/149‑1 (MQ=255)
                                    gaggagATCGGGGCGCAGGCAACAGGCGCACACTAAAGTCAGAGGGAGGGGGGGATGTTGGCGATTGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAtt                                                                                                                  <  1:333044/150‑1 (MQ=255)
                                     aggagATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCAttt                                                                                                                 >  2:579975/1‑150 (MQ=255)
                                          aTCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAgg                                                                                                            <  1:995367/150‑1 (MQ=255)
                                           tCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGGGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCGAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGc                                                                                                           <  1:1234406/150‑1 (MQ=255)
                                           tCGTGGCGCAGCCAAAAGGCGCACACTAAAGTCAGCGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGc                                                                                                           <  2:1454553/150‑1 (MQ=255)
                                            cGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGcc                                                                                                          <  1:579975/150‑1 (MQ=255)
                                             gTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTGGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCg                                                                                                         <  2:1039220/150‑1 (MQ=255)
                                                   cAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAg                                                                                                   >  2:131689/1‑150 (MQ=255)
                                                      gCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCg                                                                                                <  2:599591/150‑1 (MQ=255)
                                                           aGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAc                                                                                           >  1:247261/1‑150 (MQ=255)
                                                            ggCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCACACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcg                                                                                          >  2:1388382/1‑150 (MQ=255)
                                                             gcgcACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcgc                                                                                         <  2:1471127/150‑1 (MQ=255)
                                                              cgcACACTAAAGTCAGCGGGAGGGGGGGAGGCTGGCGACTGTTGGCGTAGGGCCCAGGGCTCCCTCGCACCCGAACCCGCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGGTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                                        <  1:816062/150‑1 (MQ=255)
                                                              cgcACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCAcgcc                                                                                        <  1:908987/150‑1 (MQ=255)
                                                                  cacTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                    >  2:160722/1‑150 (MQ=255)
                                                                       aaGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                               >  2:404077/1‑150 (MQ=255)
                                                                        aGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                              <  2:124371/150‑2 (MQ=39)
                                                                         gTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAc                                                                             <  2:72229/150‑1 (MQ=255)
                                                                          tCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGCCCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAct                                                                            >  2:1340297/1‑150 (MQ=255)
                                                                           cAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCActc                                                                           >  2:585256/1‑150 (MQ=255)
                                                                             gagTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCtt                                                                         >  2:569775/1‑150 (MQ=255)
                                                                              agTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCttt                                                                        >  2:991628/1‑150 (MQ=255)
                                                                              agTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCttt                                                                        <  1:1194801/150‑1 (MQ=255)
                                                                                ggAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCa                                                                      <  1:1230027/149‑1 (MQ=255)
                                                                                 gAGGGGGGGGTGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGAAAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAg                                                                     <  2:659334/150‑1 (MQ=255)
                                                                                 gAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAg                                                                     <  2:1293971/150‑1 (MQ=255)
                                                                                  aGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGGGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGc                                                                    <  1:855464/150‑1 (MQ=255)
                                                                                    ggggggATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTATGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCaa                                                                  >  2:631663/1‑150 (MQ=255)
                                                                                      ggggATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACa                                                                >  1:1102992/1‑150 (MQ=255)
                                                                                             gtGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGGGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAg                                                         <  1:1604556/149‑1 (MQ=255)
                                                                                              tGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGc                                                        >  2:211799/1‑150 (MQ=255)
                                                                                                 cGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGACCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGa                                                     <  2:832040/150‑1 (MQ=255)
                                                                                                 cGAATGTTGGATTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCGCGGTGGGGCGCGGGGACTGCCCGAGCGCGTTGATAGCATTTGTAGGCCGGATACGGCGTTAACGCCGCGTCCGGCACTCTTTCACTAAGATGGGTAGCggt                                                     >  2:454427/1‑149 (MQ=255)
                                                                                                    aTGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCTTTCAGCAACATGGTTAGCGGAGGc                                                  <  1:1334515/150‑1 (MQ=255)
                                                                                                    aTGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGc                                                  >  1:1347199/1‑150 (MQ=255)
                                                                                                    aTGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGc                                                  <  1:1340297/150‑1 (MQ=255)
                                                                                                    aTGTTGGCTTAGAGCCCATGGTGCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATACGGCGGTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGc                                                  <  1:225398/150‑1 (MQ=39)
                                                                                                     tGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGcc                                                 >  2:545199/1‑150 (MQ=255)
                                                                                                        tGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAg                                              >  2:905002/1‑150 (MQ=255)
                                                                                                          gCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGAt                                            >  1:1032662/1‑150 (MQ=255)
                                                                                                                 gCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAt                                     >  1:1243828/1‑150 (MQ=255)
                                                                                                                 gCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGAt                                     <  2:247261/150‑1 (MQ=255)
                                                                                                                   ccAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTc                                   <  2:1246870/150‑1 (MQ=255)
                                                                                                                    cAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCa                                  >  1:1697864/1‑150 (MQ=255)
                                                                                                                        gTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTgg                              <  2:52058/150‑1 (MQ=255)
                                                                                                                        gTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTgg                              <  2:1474175/150‑1 (MQ=255)
                                                                                                                        gTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTgg                              <  1:131689/150‑1 (MQ=255)
                                                                                                                        gTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTgg                              <  1:585256/150‑1 (MQ=255)
                                                                                                                         ttCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGc                             <  1:834314/150‑1 (MQ=255)
                                                                                                                         ttCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGc                             <  1:828503/150‑1 (MQ=255)
                                                                                                                         ttCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGc                             <  1:1604174/150‑1 (MQ=255)
                                                                                                                         ttCCCTCACACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGc                             <  1:1388382/150‑1 (MQ=255)
                                                                                                                          tCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCa                            >  2:1043148/1‑150 (MQ=255)
                                                                                                                             ctctCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaaaa                         >  2:440079/1‑150 (MQ=255)
                                                                                                                             ctctCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCaaaa                         >  2:536178/1‑150 (MQ=255)
                                                                                                                             ctctCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGCCACTCTTTCAGCAACAGGGTTAGCGGAGGCCAAGATGTTTTATTCACTCGCaaaa                         >  2:29684/1‑150 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                   <  1:160722/150‑1 (MQ=255)
                                                                                                                                   cccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGa                   >  1:1655597/1‑150 (MQ=255)
                                                                                                                                    ccTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGAGGTTTGATTCACTGGCAAAAGCCGGaa                  >  1:243611/1‑150 (MQ=255)
                                                                                                                                     cTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAg                                                    >  1:317516/1‑115 (MQ=255)
                                                                                                                                     cTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAg                                                    <  2:317516/115‑1 (MQ=255)
                                                                                                                                      tAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGTCAAGATGTTTGATTCACTGGCAAAAGCCGGaaaa                >  2:1124682/1‑150 (MQ=255)
                                                                                                                                      tAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGaaaa                <  1:211799/150‑1 (MQ=255)
                                                                                                                                        ccccTCGCCCCACCGGGGAGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAata              >  2:874230/2‑150 (MQ=39)
                                                                                                                                        aCCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAata              >  1:25612/1‑150 (MQ=255)
                                                                                                                                        aCCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAata              <  1:404077/150‑1 (MQ=255)
                                                                                                                                         cccTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAatat             >  1:1436183/1‑150 (MQ=255)
                                                                                                                                         cccTCGCCCCACCGGGGAGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAatat             >  1:469595/1‑150 (MQ=39)
                                                                                                                                            tctcCGCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGTAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTa          <  2:495407/150‑1 (MQ=255)
                                                                                                                                               ccccGGTTGGGCGAGGGGACTGACAGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTAAATTGCAAAAGCCGGAAAATATTTAGGt       >  1:451395/1‑150 (MQ=39)
                                                                                                                                                 ccGGTGTGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGCTCACGCCGCATGCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCa     <  2:125473/150‑1 (MQ=255)
                                                                                                                                                   ggTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAgg   <  1:1205359/150‑1 (MQ=255)
                                                                                                                                                    gTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAggc  >  2:1526029/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAAGAAACGCCGTATGAACCAATGCCGGAAAATGTCGAGGAGATCGTGGCGCAGGCAAAAGGCGCACACTAAAGTCAGAGTGAGGGGGGGATGTTGGCGAATGTTGGCTTAGTGCCCAGGGTTCCCTCTCACCCTAACCCTCTCCCCGGTGGGGCGAGGGGACTGACCGAGCGCGTTGATAGCATTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACTCTTTCAGCAACATGGTTAGCGGAGGCCAAGATGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGC  >  NC_000913/631928‑632225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: