Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 25,781 T→A 6.6% intergenic (+80/‑45) lspA → / → fkpB prolipoprotein signal peptidase (signal peptidase II)/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091325,7810TA 6.6% 400.0 / 12.5 135intergenic (+80/‑45)lspA/fkpBprolipoprotein signal peptidase (signal peptidase II)/FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base T (67/59);  new base A (2/7);  total (69/66)
Fisher's exact test for biased strand distribution p-value = 9.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 136 total aligned reads displayed.

TGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAAC  >  NC_000913/25632‑25925
                                                                                                                                                       |                                                                                                                                                
tGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGttt                                                                                                                                                  >  1:478480/1‑150 (MQ=255)
       tgtgTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTAcgccgc                                                                                                                                           <  1:1699978/150‑1 (MQ=255)
           tCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATcc                                                                                                                                       <  2:1498243/150‑1 (MQ=255)
               tGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATGCG‑‑GCGTAAACGCCTTATCCGGcc                                                                                                                                   <  1:523646/150‑2 (MQ=39)
                  ggCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGTGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATGCG‑‑GCGTAAACGCCTTATCCGGcctac                                                                                                                                <  2:961893/150‑5 (MQ=255)
                  ggCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCTAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCaaaa                                                                                                                                >  1:1183555/1‑150 (MQ=255)
                     aCTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATc                                                                                                                             >  1:333242/1‑150 (MQ=255)
                     aCTGAATGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGACTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATc                                                                                                                             <  2:1117144/150‑1 (MQ=255)
                         aTTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTa                                                                                                                         <  1:1554914/150‑1 (MQ=255)
                              gCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                                                                                                    >  2:1694093/1‑150 (MQ=255)
                              gCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                                                                                                    >  1:661948/1‑150 (MQ=255)
                              gCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                                                                                                    >  1:1011245/1‑150 (MQ=255)
                               cTGGAAGGTTTTTTGCCTTCTACACCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACCCATCTTATCCGGCCTACAGATTGCTTCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCATAAAtata                                                                                                                   >  2:1256737/1‑150 (MQ=37)
                                tGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATaa                                                                                                                  >  1:417439/1‑150 (MQ=255)
                                tGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATaa                                                                                                                  >  2:1173365/1‑150 (MQ=255)
                                 ggAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAag                                                                                                                 >  1:1350155/1‑150 (MQ=255)
                                 ggAAGGTTTTTTGACTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAag                                                                                                                 <  1:742794/150‑1 (MQ=255)
                                  gAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGTCGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTaaa                                                                                                                       >  2:1152459/1‑143 (MQ=255)
                                  gAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGTCGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTaaa                                                                                                                       <  1:1152459/143‑1 (MQ=255)
                                  gAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGTCGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTaaa                                                                                                                       <  1:1186562/143‑1 (MQ=255)
                                  gAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGTCGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTaaa                                                                                                                       >  2:1186562/1‑143 (MQ=255)
                                   aaGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAagag                                                                                                               <  2:112855/150‑1 (MQ=255)
                                     ggTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCa                                                                                                             <  1:1484450/150‑1 (MQ=255)
                                      gTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCaa                                                                                                            >  1:1429347/1‑150 (MQ=255)
                                        tttttGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAc                                                                                                          <  1:447299/150‑1 (MQ=255)
                                             gCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCa                                                                                                     >  1:967578/1‑150 (MQ=255)
                                             gCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCa                                                                                                     <  2:139712/150‑1 (MQ=255)
                                              ccTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCAt                                                                                                    >  1:1587431/1‑150 (MQ=255)
                                               cTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATg                                                                                                   <  2:463114/150‑1 (MQ=255)
                                                   tAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTg                                                                                               <  2:538230/150‑1 (MQ=255)
                                                      agCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAAt                                                                                            >  2:20467/1‑150 (MQ=255)
                                                      agCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGAATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTATATATAAGAGCAAACCTGCATGTCTGAAt                                                                                            <  1:561555/150‑1 (MQ=255)
                                                        cGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCt                                                                                          >  2:1067159/1‑150 (MQ=255)
                                                        cGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCt                                                                                          >  1:582288/1‑150 (MQ=255)
                                                          aaaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACTCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGt                                                                                        <  2:208510/150‑1 (MQ=255)
                                                          aaaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGt                                                                                        >  1:933098/1‑150 (MQ=255)
                                                           aaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa                                                                                       <  1:390743/150‑1 (MQ=255)
                                                           aaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa                                                                                       <  2:1709610/150‑1 (MQ=255)
                                                           aaaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTa                                                                                       <  1:384404/150‑1 (MQ=255)
                                                            aaaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAc                                                                                      >  2:539084/1‑150 (MQ=255)
                                                             aaaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAATCCTGCATGTCTGAATCTGTACa                                                                                     <  2:1456178/150‑1 (MQ=255)
                                                              aaCAATAATAAACCCTGCCGTATGCGATGCTGACGCATCTTATACGTCCTACAGATTTCTGAGAAATCGTAGTCCGGATAAG‑‑TCGTTTTCGCCGCATACGGCAAAAATCCTTAAATATATGAGCAAACCTGCATGTCTGAATCTGTACag                                                                                    <  1:424327/150‑1 (MQ=255)
                                                              aaCAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCGGGCCGACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAACTCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACag                                                                                    <  2:865071/150‑1 (MQ=255)
                                                                cAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAGCTGCATGTCTGAATCTCTACagag                                                                                  >  1:265793/1‑150 (MQ=255)
                                                                cAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACagag                                                                                  >  2:1621316/1‑150 (MQ=255)
                                                                cAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATGGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAACGCCTTAAATATAAGAGCACACCTGCATGTCTGAATCTGTACagcg                                                                                  >  1:1662312/1‑148 (MQ=255)
                                                                aaataatAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACagag                                                                                  <  2:1415606/149‑1 (MQ=255)
                                                                  ataataAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATAGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGACCAAACCTGCATGTCTGAATCTGTACAGAGCa                                                                                >  2:1384366/1‑150 (MQ=255)
                                                                   taataaACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCaa                                                                               >  1:1326678/1‑150 (MQ=255)
                                                                      taaACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GAGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAg                                                                            >  2:779049/1‑150 (MQ=255)
                                                                           ccTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCg                                                                       <  2:1295697/150‑1 (MQ=255)
                                                                                cGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTg                                                                  >  2:912787/1‑150 (MQ=255)
                                                                                   atgcgatgcTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTg                                                               <  2:310211/150‑1 (MQ=255)
                                                                                    tgcgatgcTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACGGAGCAATAGCGCCGTCCTGGTGc                                                              <  1:1341638/150‑1 (MQ=255)
                                                                                      cgatgcTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAGACCTGCATGTCTGAATCAGTACAGAGCAATAGCGCCGTCCTGGTGCag                                                            >  1:1072115/1‑149 (MQ=255)
                                                                                      cgatgcTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCAc                                                            >  2:1393170/1‑150 (MQ=255)
                                                                                         tgcTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGGTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTc                                                         <  2:117298/150‑1 (MQ=255)
                                                                                          gcTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTcc                                                        >  1:711120/1‑149 (MQ=255)
                                                                                           cTGACGCAGCTTATCCGGCCTACAGATTGCTGCGATATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCACACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCAc                                                       <  1:1297020/150‑1 (MQ=255)
                                                                                            tGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACg                                                      >  1:512388/1‑150 (MQ=255)
                                                                                            tGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACg                                                      <  2:1394189/150‑1 (MQ=255)
                                                                                              aCGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCt                                                    <  1:1447489/150‑1 (MQ=255)
                                                                                                  aTCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTaaaa                                                >  1:764315/1‑150 (MQ=255)
                                                                                                        tCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTcgac                                          <  2:1587431/150‑1 (MQ=255)
                                                                                                        tCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTcgac                                          <  1:868930/150‑1 (MQ=255)
                                                                                                         ccGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTcgacg                                         <  2:1462942/150‑1 (MQ=255)
                                                                                                         ccGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTcgacg                                         <  2:1447938/150‑1 (MQ=255)
                                                                                                         ccGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTcgacg                                         <  1:1021485/150‑1 (MQ=255)
                                                                                                           ggCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGAt                                       >  2:1008820/1‑150 (MQ=255)
                                                                                                            gCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCCCTTCACGCTAAAACTCGACGATg                                      >  2:319688/1‑150 (MQ=255)
                                                                                                            gCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATg                                      >  1:1157569/1‑150 (MQ=255)
                                                                                                             ccTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATgg                                     <  1:1694093/150‑1 (MQ=255)
                                                                                                                 cAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGcacc                                 <  2:394006/150‑1 (MQ=255)
                                                                                                                  aGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGcacca                                <  2:47412/150‑1 (MQ=255)
                                                                                                                  aGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGcacca                                >  2:189221/1‑150 (MQ=255)
                                                                                                                        ctgcGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCTTGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCCCGCTAAAACTCGACGATGGCACCAccgccg                          >  1:1511882/1‑150 (MQ=255)
                                                                                                                          gcGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATgg                                     <  1:1658899/137‑1 (MQ=255)
                                                                                                                          gcGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATgg                                     >  2:1658899/1‑137 (MQ=255)
                                                                                                                          gcGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAg                        <  1:1173365/150‑1 (MQ=255)
                                                                                                                            gAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGGCGGTGGTGCCATCGTCGAGtt                      >  2:1496074/1‑149 (MQ=38)
                                                                                                                             aatctgTAGGCCGGATAAGATGCGTCAGCATCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGt                       <  1:713149/145‑1 (MQ=37)
                                                                                                                              aaTCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTa                    <  2:1350155/150‑1 (MQ=255)
                                                                                                                               aTCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGGCGGTGGTGCCATCGTCGAGtttta                   <  1:1496074/150‑5 (MQ=38)
                                                                                                                                tCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTAcc                  <  2:1429347/150‑1 (MQ=255)
                                                                                                                                  gTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCg                <  1:1562467/150‑1 (MQ=255)
                                                                                                                                     tgCCGGATGCG‑‑GCGTAAACGCCTTATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTAGACGATGGCAACACCGCCGAGTCTACCCGcaa             >  2:1629373/2‑150 (MQ=255)
                                                                                                                                     ggCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCCCCGCCGAGTCTACCCGcaa             >  1:510786/1‑150 (MQ=255)
                                                                                                                                      gCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCTCCTCCGCCGAGTCTACACGcaac            >  1:228726/1‑150 (MQ=255)
                                                                                                                                      gCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGcaac            <  1:176852/150‑1 (MQ=255)
                                                                                                                                       ccGGATGCG‑‑GCGTAAACGCCTTATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGcaac            >  2:669712/1‑149 (MQ=255)
                                                                                                                                        cGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGcaacaa          >  2:636707/1‑150 (MQ=255)
                                                                                                                                        cGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTATGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGcaacaa          >  2:630764/1‑150 (MQ=255)
                                                                                                                                         ggATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCaacaac         >  1:129463/1‑150 (MQ=255)
                                                                                                                                         ggATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCaacaac         <  1:1393170/150‑1 (MQ=255)
                                                                                                                                         ggATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCaacaac         >  2:640696/1‑150 (MQ=255)
                                                                                                                                            tAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCt                     <  1:1148084/135‑1 (MQ=255)
                                                                                                                                            tAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGt      <  1:1709947/150‑1 (MQ=255)
                                                                                                                                             aaG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTa     <  1:481763/150‑1 (MQ=255)
                                                                                                                                               g‑‑gCGTTTATGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTaaa   >  2:1480911/1‑150 (MQ=255)
                                                                                                                                                  gCGTTTACGCCGCATCCGTCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAAc  <  2:582288/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                
TGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAG‑‑GCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAAC  >  NC_000913/25632‑25925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: