Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 693,023 T→G 5.3% K119T (AAG→ACG)  ybeZ ← heat shock protein, putative NTPase; PhoH‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913693,0230TG 5.3% 262.2 / 4.6 94K119T (AAG→ACG) ybeZheat shock protein, putative NTPase; PhoH‑like protein
Reads supporting (aligned to +/- strand):  ref base T (38/50);  new base G (2/3);  total (41/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

GAATACGGCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCA  >  NC_000913/692874‑693169
                                                                                                                                                     |                                                                                                                                                  
gAATACGGCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCt                                                                                                                                                    >  1:500492/1‑150 (MQ=255)
 actACGGCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCtt                                                                                                                                                   <  1:582452/148‑1 (MQ=255)
 aaTACGGCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCtt                                                                                                                                                   <  2:227268/150‑1 (MQ=255)
   tACGGCGAATATCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGATTATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTaa                                                                                                                                                 <  1:1136180/150‑1 (MQ=255)
      ggCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGGAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTa                                                                                                                                              >  1:270573/1‑150 (MQ=255)
       gCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAc                                                                                                                                             >  2:99477/1‑150 (MQ=255)
        cGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTAcg                                                                                                                                            >  2:116806/1‑150 (MQ=255)
               ccTGACGCTCCAGGGCATCAACTGCCGCAGCAACTTCCAGGTAGGTTTTACCCGTACCCGCCGGGCCACCGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGAACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTTCGCCGCGtt                                                                                                                                     >  1:540206/1‑150 (MQ=255)
                cTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcac                                                                                                                                    <  1:769547/150‑4 (MQ=255)
                cTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcac                                                                                                                                    <  2:55981/150‑4 (MQ=255)
                 tGACGCTCCAGGGCATCACCTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGCTGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAAGTACGCCGCGGTTg                                                                                                                                   >  1:1021796/1‑150 (MQ=255)
                 tGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCTTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTAAGCcgcgcacg                                                                                                                                   <  2:149543/150‑5 (MQ=255)
                  gACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTgg                                                                                                                                  >  1:1418784/1‑150 (MQ=255)
                       tCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTg                                                                                                                             <  1:1708446/150‑1 (MQ=255)
                       tCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTg                                                                                                                             >  2:1655469/1‑150 (MQ=255)
                         cAGGGCATCAACTGCCGCAGCCACTGCCATGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGATTATTGGCTATTTACTGCGCCTGGTTTGGCGTGCGCGGCTTTATTACGCCGCGTTTGGTTTTGat                                                                                                                           <  2:968863/150‑1 (MQ=255)
                         cAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGat                                                                                                                           <  1:1205925/150‑1 (MQ=255)
                         cAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGat                                                                                                                           >  2:374114/1‑150 (MQ=255)
                           gggCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGatat                                                                                                                         >  2:421818/1‑150 (MQ=255)
                            ggCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATAtt                                                                                                                        >  2:877507/1‑150 (MQ=255)
                              cATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGat                                                                                                                           <  2:771617/145‑1 (MQ=255)
                              cATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGat                                                                                                                           >  1:771617/1‑145 (MQ=255)
                               aTCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCAGTACCCGCCGGGCCAACGCCGTAGGTAATGTCAAGGTCGAGAATATTGGCGATGTACTGCGCGTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGATTTGATATTGAc                                                                                                                     >  2:25257/1‑150 (MQ=255)
                                tCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGAcc                                                                                                                    <  2:549518/150‑1 (MQ=255)
                                  aaCTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGc                                                                                                                  >  1:876359/1‑150 (MQ=255)
                                      gCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGCAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTTGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCtttt                                                                                                              >  2:844191/1‑149 (MQ=255)
                                        cGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGcc                                                                                                            >  2:915119/1‑150 (MQ=255)
                                         gCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCg                                                                                                           <  2:866066/150‑1 (MQ=255)
                                         gCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCg                                                                                                           >  2:1222427/1‑150 (MQ=255)
                                             ccACTGCCAGGTAGGTTTTACCCGTACGCGCCGTGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACt                                                                                                       <  1:993556/150‑1 (MQ=255)
                                                 tGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCgg                                                                                                   <  2:1196998/150‑1 (MQ=255)
                                                    cAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCAc                                                                                                <  1:782200/150‑1 (MQ=255)
                                                      ggtaggtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGc                                                                                              >  2:654245/1‑150 (MQ=255)
                                                         aggtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGGAGTACGGCaaagcgg                                                                                           <  2:1496133/150‑7 (MQ=255)
                                                          ggtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCtctc                                                                                          >  1:964359/1‑150 (MQ=255)
                                                          cgtTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCATAATTAAGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCtctc                                                                                          >  1:1276756/2‑150 (MQ=255)
                                                            ttttACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCcg                                                                                        <  1:951331/150‑1 (MQ=255)
                                                            ttttACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGGCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCcg                                                                                        <  1:1221735/150‑1 (MQ=255)
                                                              ttACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCcgcg                                                                                      <  2:1418784/150‑1 (MQ=255)
                                                                acccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCt                                                                                    <  1:696833/150‑1 (MQ=255)
                                                                 cccgtacccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCtt                                                                                   >  1:1418657/1‑150 (MQ=255)
                                                                      acccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTc                                                                              >  1:653241/1‑150 (MQ=255)
                                                                      acccgCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTc                                                                              <  1:116806/150‑1 (MQ=255)
                                                                       cccgCCGGGCCAACGCCGAAGGGAATGGCATGGGCGAGAATATGGGCGCTGGACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTcc                                                                             <  1:76862/150‑1 (MQ=255)
                                                                        ccgccgGGCCAACGCCGAAGGTAATGTCCTGGTCGAGAATATTGGCGCTGTACTGCGCCTGGTTTGGCGGGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCa                                                                            <  2:1581895/150‑1 (MQ=255)
                                                                           ccgGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTa                                                                         >  1:1487465/1‑150 (MQ=255)
                                                                             tggCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTAcc                                                                       >  1:1052247/2‑150 (MQ=255)
                                                                             gggCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTAcc                                                                       >  2:394184/1‑150 (MQ=255)
                                                                                 cAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCcgcg                                                                   >  1:654895/1‑150 (MQ=255)
                                                                                 cAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAAATAAGCCGCGGTTGGTTTTGATATTGACCGCTTAGCCGTTCTCCGTCACGCACTCCGCGCTTTGCTCCAGTACCcgcg                                                                   >  2:1400486/1‑150 (MQ=255)
                                                                                  aaCGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCgcgc                                                                  >  1:218840/1‑150 (MQ=255)
                                                                                      ccGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCGTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGcttct                                                              >  2:1426287/1‑150 (MQ=255)
                                                                                         aaGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTa                                                           <  1:421818/150‑1 (MQ=255)
                                                                                            gTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATc                                                        <  2:464188/150‑1 (MQ=255)
                                                                                            gTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATc                                                        <  1:43944/150‑1 (MQ=255)
                                                                                             tAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCg                                                       <  2:385279/150‑1 (MQ=255)
                                                                                             tAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCg                                                       <  2:500492/150‑1 (MQ=255)
                                                                                                  tCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAgg                                                  <  2:1418657/150‑1 (MQ=255)
                                                                                                   cATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCTGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGt                                                 <  2:334636/150‑1 (MQ=255)
                                                                                                   cATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGt                                                 <  2:654895/150‑1 (MQ=255)
                                                                                                     tGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATGTTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTgg                                               >  2:634786/1‑150 (MQ=255)
                                                                                                       gTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGAt                                             <  2:335838/150‑1 (MQ=255)
                                                                                                         cGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATc                                                        <  1:494294/137‑1 (MQ=255)
                                                                                                         cGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATc                                                        >  2:494294/1‑137 (MQ=255)
                                                                                                         cGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCt                                           <  1:758554/150‑1 (MQ=255)
                                                                                                             aaTATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTc                                       <  1:187405/150‑1 (MQ=255)
                                                                                                              attttGGCGATGTACTGCGCCTGGTTTGGAGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGATTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCATTACCCGCTCTTCTTTAATCGCAATGTGGATCTGttac                                      <  2:540206/147‑3 (MQ=255)
                                                                                                              atatTGGCGATGTACTGCGGCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTcc                                      <  1:547426/150‑1 (MQ=255)
                                                                                                              atatTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTcc                                      <  2:270573/150‑1 (MQ=255)
                                                                                                              atatTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTcc                                      >  2:94183/1‑150 (MQ=255)
                                                                                                              atatTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTcc                                      >  1:1370467/1‑150 (MQ=255)
                                                                                                              atatTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGAACGCTTTGCCGTACTCCCGCACCCACTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATAGCAAGGTGGATCTGTTcc                                      >  1:1304419/1‑150 (MQ=255)
                                                                                                                 ttGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGt                                   <  1:1699920/150‑1 (MQ=255)
                                                                                                                  tGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGtt                                  >  2:1135031/1‑150 (MQ=255)
                                                                                                                  tGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGtt                                  <  2:1370467/150‑1 (MQ=255)
                                                                                                                   ggCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTc                                 <  1:899002/150‑1 (MQ=255)
                                                                                                                    gCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCg                                <  1:654245/150‑1 (MQ=255)
                                                                                                                     cGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGa                               <  1:99477/150‑1 (MQ=255)
                                                                                                                       aTGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGata                             <  2:653241/150‑1 (MQ=255)
                                                                                                                            cTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTg                        <  1:877507/150‑1 (MQ=255)
                                                                                                                             tGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGa                       >  1:233571/1‑150 (MQ=255)
                                                                                                                              gcgcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGaa                      <  2:1052247/150‑1 (MQ=255)
                                                                                                                              gcgcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGaa                      <  1:428051/150‑1 (MQ=255)
                                                                                                                              gcgcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGGTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGaa                      <  2:623161/150‑1 (MQ=255)
                                                                                                                               cgcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAAt                     <  2:964359/150‑1 (MQ=255)
                                                                                                                                gcCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATc                    <  1:915119/150‑1 (MQ=255)
                                                                                                                                         ggCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCgcgc           >  1:709027/1‑150 (MQ=255)
                                                                                                                                         ggCGTGCGCGGCTTAATTACGCCGCGGTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTCGATCTGTTACGGTTCGATATCCTGAATCTGACCgcgc           >  1:60857/1‑150 (MQ=255)
                                                                                                                                          gCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCa          >  2:489894/1‑150 (MQ=255)
                                                                                                                                          ccaaacgcgGCGTAATTAAGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTACGCGCTTTGCTCCAGTACCCGGGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCa          >  1:51965/6‑150 (MQ=255)
                                                                                                                                             tGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCg       >  2:52321/1‑150 (MQ=255)
                                                                                                                                               cgcgGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTACGATATCCTGAATCTGACCGCGCATCggg     >  2:927111/1‑150 (MQ=255)
                                                                                                                                                gcgGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCgggg    <  2:266297/150‑1 (MQ=255)
                                                                                                                                                gcgGCTTAATTACGCAGCGTTTGGTTTTGATATTGACCGCTTTGCCGTAGTCCGGGACGCTCTCCGCGCTTTGCTCGAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCgggg    <  2:638240/150‑1 (MQ=255)
                                                                                                                                                 cgGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGc   <  2:556197/150‑1 (MQ=255)
                                                                                                                                                 cgGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTGCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGc   <  2:1299512/150‑1 (MQ=255)
                                                                                                                                                  ggCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGGGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCa  <  1:94183/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GAATACGGCGAATTTCCTGACGCTCCAGGGCATCAACTGCCGCAGCCACTGCCAGGTAGGTTTTACCCGTACCCGCCGGGCCAACGCCGAAGGTAATGTCATGGTCGAGAATATTGGCGATGTACTGCGCCTGGTTTGGCGTGCGCGGCTTAATTACGCCGCGTTTGGTTTTGATATTGACCGCTTTGCCGTACTCCGGCACGCTCTCCGCGCTTTGCTCCAGTACCCGCGCTTCTTTAATCGCAAGGTGGATCTGTTCCGGTTCGATATCCTGAATCTGACCGCGCATCGGGGCA  >  NC_000913/692874‑693169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: