Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 697,487 A→T 6.2% intergenic (‑354/+26) metT ← / ← asnB tRNA‑Met/asparagine synthetase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913697,4870AT 6.2% 309.1 / 5.8 106intergenic (‑354/+26)metT/asnBtRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base A (51/48);  new base T (4/3);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACG  >  NC_000913/697339‑697636
                                                                                                                                                    |                                                                                                                                                     
ttCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGAt                                                                                                                                                      <  1:1176851/150‑1 (MQ=255)
  cGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGAttt                                                                                                                                                    >  2:520951/1‑150 (MQ=255)
      aTTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCAcc                                                                                                                                                <  1:1106206/150‑1 (MQ=255)
        ttAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTAGAGTGATGAGTTGTCAAACAGTGATGTGGCAAACACGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCgg                                                                                                                                              <  2:1495854/150‑1 (MQ=255)
            cAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCt                                                                                                                                          <  1:289702/150‑1 (MQ=255)
               tAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGtt                                                                                                                                       <  1:321742/150‑1 (MQ=255)
                   ggtggGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGTGAAATCCGGGGCTGTTTcag                                                                                                                                   <  1:1364172/150‑3 (MQ=255)
                    gttggAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGTGAAATCCGGGGCTGTTTcagt                                                                                                                                  <  1:1334603/147‑4 (MQ=255)
                      gggAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGTGAAATCCGGGGCTGTTTcagt                                                                                                                                  >  1:251017/1‑145 (MQ=39)
                      gggAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGTGAAATCCGGGGCTGTTTcagt                                                                                                                                  <  2:251017/148‑4 (MQ=39)
                       ggAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCAttt                                                                                                                               >  2:1620791/1‑150 (MQ=255)
                        gAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTc                                                                                                                              <  2:635308/150‑1 (MQ=255)
                         aaaTTAATATATGAAATACTGGCCTACAGTGATGAGTTGTCATACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCTACAAGCACCTCAATAACTGAATCAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTct                                                                                                                             <  2:951227/150‑1 (MQ=255)
                          aaTTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTctt                                                                                                                            >  1:939499/1‑150 (MQ=255)
                           aTTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTctta                                                                                                                           <  1:174551/150‑1 (MQ=255)
                            ttAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttac                                                                                                                          <  2:332096/150‑1 (MQ=255)
                               aTCTATGAAATACTGGCCTACCGTGATGAGTTGTCAAACAGTGAGGTGGCAAACCCTGAACATTTCCTTACTGCATATCAGAATCAACATGCACCTCAATACCTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       <  2:278254/150‑1 (MQ=255)
                               aTCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       <  2:1265579/150‑1 (MQ=255)
                               aTCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       <  2:1510111/150‑1 (MQ=255)
                                 cTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACATCCCCGGATTTCACCGGGGCTGTTTCTAATTTCTTACTtata                                                                                                                     >  2:1571458/1‑150 (MQ=255)
                                 cTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTtata                                                                                                                     <  2:553957/150‑1 (MQ=255)
                                  tATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATAc                                                                                                                    >  1:1287847/1‑150 (MQ=255)
                                   aTGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACg                                                                                                                   >  2:1356322/1‑150 (MQ=255)
                                          aCTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTg                                                                                                            >  2:8230/1‑150 (MQ=255)
                                           cTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTgg                                                                                                           >  2:203458/1‑150 (MQ=255)
                                              gCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCTACTGGTGa                                                                                                        >  2:1577710/1‑150 (MQ=255)
                                                 tACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       >  2:797685/1‑132 (MQ=255)
                                                 tACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       <  1:797685/132‑1 (MQ=255)
                                                 tACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAaca                                                                                                     >  1:1061253/1‑150 (MQ=255)
                                                  aCAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAacac                                                                                                    <  2:939499/150‑1 (MQ=255)
                                                    aGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCa                                                                                                  <  1:8230/150‑1 (MQ=255)
                                                     gTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGACCACCaa                                                                                                 >  2:1714081/1‑150 (MQ=255)
                                                         tgaGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAACAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgc                                                                                             >  1:650221/1‑150 (MQ=255)
                                                            gTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcgcg                                                                                          <  1:908679/150‑1 (MQ=255)
                                                            gTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcgcg                                                                                          >  1:405910/1‑150 (MQ=255)
                                                            gTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcgcg                                                                                          <  1:810428/150‑1 (MQ=255)
                                                             ttGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcgcgt                                                                                         <  1:1078068/150‑2 (MQ=255)
                                                               gTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGa                                                                                                        <  1:1080049/133‑1 (MQ=255)
                                                               gTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGa                                                                                                        >  2:1080049/1‑133 (MQ=255)
                                                               gTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGAcc                                                                                       >  1:1237211/1‑150 (MQ=255)
                                                               gTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGAcc                                                                                       >  2:261704/1‑150 (MQ=255)
                                                                 cAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGTATCAACAAGCTCCTCAATAACTGAAACAGCCCCGGATGTCGGCGGGGCTGTGTCGCGTTTCTTACTCAAACGCCGACTGGTGCACACCAACCGAGCGccccg                                                                                     >  2:162527/1‑148 (MQ=255)
                                                                 cAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAg                                                                                     >  1:1638138/1‑150 (MQ=255)
                                                                 cAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAg                                                                                     >  2:1638109/1‑150 (MQ=255)
                                                                  aaaCAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGa                                                                                    >  2:860339/1‑150 (MQ=255)
                                                                   aaCAGTGATGTCGCAAACACGGAAGATTTCCTTAGTGCATAGCTGAATCAAGAAGCACCTCAATTACTGAAACTGCCCCTGATTTCACCGGTGCTGTTTCGCATTGCTTACTTATACGCCGACTGGTGAACTCCTACCGCGCGACCAGAc                                                                                   <  1:251023/150‑1 (MQ=255)
                                                                    aCAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACg                                                                                  >  2:1241212/1‑150 (MQ=255)
                                                                       gTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGAt                                                                               <  1:984837/150‑1 (MQ=255)
                                                                         gATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCg                                                                             >  2:712255/1‑150 (MQ=255)
                                                                          aTGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCAGCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGAGAACACCAACCGCGCGACCAGACGGATCGt                                                                            <  2:394656/150‑1 (MQ=255)
                                                                          aTGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGt                                                                            <  1:1620791/150‑1 (MQ=255)
                                                                              ggCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAt                                                                        >  2:1495667/1‑150 (MQ=255)
                                                                              ggCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAt                                                                        >  1:493713/1‑150 (MQ=255)
                                                                              ggCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAt                                                                        >  1:500245/1‑150 (MQ=255)
                                                                              ggCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAt                                                                        >  2:468440/1‑150 (MQ=255)
                                                                                cAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAACAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAttt                                                                      >  1:552174/1‑150 (MQ=255)
                                                                                    cccGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCtt                                                                  >  1:1252149/1‑150 (MQ=255)
                                                                                        gAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAAc                                                              <  1:1241212/150‑1 (MQ=255)
                                                                                         aaCATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACg                                                             <  1:1638109/150‑1 (MQ=255)
                                                                                           cATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCt                                                           >  1:1628297/1‑150 (MQ=255)
                                                                                           cATTTCCGTACTGCATATCAGAATCAACAAGAACCTCAATAACTGAAACAGCCCCGGATTACACCGGGCCTGTTTCGCAGTTCTTACTTATACGCAGACTGGTGCACACCAACCGCGCGACCAGCAGGATCGTCCTGTATCGTGAACGcg                                                           >  1:107917/1‑149 (MQ=255)
                                                                                             tttCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTc                                                         >  1:629316/1‑150 (MQ=255)
                                                                                                ccTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATc                                                      <  2:1671866/150‑1 (MQ=255)
                                                                                                     cTGCATATCAGAATCAACATGCACCTGAATAAGTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGATCACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCAtt                                                 <  2:247609/150‑1 (MQ=255)
                                                                                                     cTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCAtt                                                 >  1:88672/1‑150 (MQ=255)
                                                                                                       gCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCg                                               >  2:665939/1‑150 (MQ=255)
                                                                                                        cATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCCCCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCCACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGa                                              >  1:707283/1‑150 (MQ=255)
                                                                                                         atatCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGAt                                             <  2:1061253/150‑1 (MQ=255)
                                                                                                          tatCAGAATCAACAATCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATc                                            <  2:1051727/150‑1 (MQ=255)
                                                                                                          tatCAGAATCAACAAGCACCACAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGa                                                                                >  2:1250180/1‑114 (MQ=255)
                                                                                                          tatCAGAATCAACAAGCACCACAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGa                                                                                <  1:1250180/114‑1 (MQ=255)
                                                                                                            tCAGAATCAACAAGCACCTCAACAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGc                                          <  2:650221/150‑1 (MQ=255)
                                                                                                              aGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTATCGCATTTCTTACTTATACGCCGACTGGTGACCACCAACCGCGCGCCCAGACGGATCGTCCATTTTATTGAACGCTTCATCCCAGTCGATCGCtt                                        >  1:136757/1‑150 (MQ=255)
                                                                                                               gAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCttt                                       <  2:405910/150‑1 (MQ=255)
                                                                                                                aaTCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTa                                      <  1:860339/150‑1 (MQ=255)
                                                                                                                  tCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGc                                    <  1:1356322/150‑1 (MQ=255)
                                                                                                                   caacaaGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGCACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCg                                   >  2:73633/1‑150 (MQ=255)
                                                                                                                    aacaaGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCgg                                  >  1:665330/1‑150 (MQ=255)
                                                                                                                           aCCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGCCCAGACGGATCGTCCATTTTCTTGAACGCTGCATCCCATTCGATCGCGTTAGCGGAAGAACa                           >  1:1616576/1‑150 (MQ=255)
                                                                                                                           aCCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACa                           <  2:1237211/150‑1 (MQ=255)
                                                                                                                              tCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGc                        <  1:665939/150‑1 (MQ=255)
                                                                                                                               cAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCg                       >  2:1075027/1‑150 (MQ=255)
                                                                                                                                    aCTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTTCTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGGTTTAGCGGTAGCACAAGCGACGGa                  >  2:198308/1‑150 (MQ=255)
                                                                                                                                     cTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGaa                 >  1:327179/1‑150 (MQ=255)
                                                                                                                                      tGAAACAGCCCCGGATTTCACCGGGGCTGCTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAg                <  1:674920/150‑1 (MQ=255)
                                                                                                                                      cgAAACAGCCCCGGTGAAATCCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAg                >  2:1007436/2‑150 (MQ=255)
                                                                                                                                      cgAAACAGCCCCGGTGAAATCCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAg                >  2:92984/2‑150 (MQ=255)
                                                                                                                                      cgAAACAGCCCCGGTGAAATCCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACAGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCCTCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAg                >  1:68281/2‑150 (MQ=255)
                                                                                                                                       gAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAgg               <  1:1495667/150‑1 (MQ=255)
                                                                                                                                       gAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAgg               <  2:88672/150‑1 (MQ=255)
                                                                                                                                       gAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCAATTCTTACTTATACGCCGACTGGTGAACACACACCGCGCGACCAGACGGATCGTCCAGTTTCTTGAACGCGTCATCCAAGTCGATCGCTTGAGCGGAAGCACAAGCGACGGAAgg               >  2:112798/1‑150 (MQ=255)
                                                                                                                                         aaCAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGAc             <  2:327179/150‑1 (MQ=255)
                                                                                                                                           cAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGCCGGAAGGACCg           >  2:1000293/1‑150 (MQ=255)
                                                                                                                                             gCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCCTCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGcc         >  1:300385/1‑150 (MQ=255)
                                                                                                                                             gCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGcc         >  1:575742/1‑150 (MQ=255)
                                                                                                                                              ccccGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGccc        <  2:1638138/150‑1 (MQ=255)
                                                                                                                                                ccGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCgg      <  1:712255/150‑1 (MQ=255)
                                                                                                                                                ccGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCgg      <  1:468440/150‑1 (MQ=255)
                                                                                                                                                  ggATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGgca    >  1:1430430/1‑150 (MQ=255)
                                                                                                                                                    aTTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGgcacg  >  2:834/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTCGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACG  >  NC_000913/697339‑697636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: