Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 697,490 T→A 6.7% intergenic (‑357/+23) metT ← / ← asnB tRNA‑Met/asparagine synthetase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913697,4900TA 6.7% 299.9 / 5.5 105intergenic (‑357/+23)metT/asnBtRNA‑Met/asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base T (50/47);  new base A (5/3);  total (55/50)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACG  >  NC_000913/697341‑697636
                                                                                                                                                     |                                                                                                                                                  
cGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGAttt                                                                                                                                                    >  2:520951/1‑150 (MQ=255)
    aTTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCAcc                                                                                                                                                <  1:1106206/150‑1 (MQ=255)
      ttAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTAGAGTGATGAGTTGTCAAACAGTGATGTGGCAAACACGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCgg                                                                                                                                              <  2:1495854/150‑1 (MQ=255)
         aCAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGc                                                                                                                                           <  2:1144295/150‑1 (MQ=255)
          cAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCt                                                                                                                                          <  1:289702/150‑1 (MQ=255)
             tAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGtt                                                                                                                                       <  1:321742/150‑1 (MQ=255)
                 ggtggGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGTGAAATCCGGGGCTGTTTcag                                                                                                                                   <  1:1364172/150‑3 (MQ=255)
                  gttggAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGTGAAATCCGGGGCTGTTTcagt                                                                                                                                  <  1:1334603/147‑4 (MQ=255)
                    gggAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGTGAAATCCGGGGCTGTTTcagt                                                                                                                                  >  1:251017/1‑145 (MQ=39)
                    gggAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGTGAAATCCGGGGCTGTTTcagt                                                                                                                                  <  2:251017/148‑4 (MQ=39)
                     ggAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCAttt                                                                                                                               >  2:1620791/1‑150 (MQ=255)
                      gAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTc                                                                                                                              <  2:635308/150‑1 (MQ=255)
                       aaaTTAATATATGAAATACTGGCCTACAGTGATGAGTTGTCATACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCTACAAGCACCTCAATAACTGAATCAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTct                                                                                                                             <  2:951227/150‑1 (MQ=255)
                        aaTTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTctt                                                                                                                            >  1:939499/1‑150 (MQ=255)
                         aTTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTctta                                                                                                                           <  1:174551/150‑1 (MQ=255)
                          ttAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttac                                                                                                                          <  2:332096/150‑1 (MQ=255)
                             aTCTATGAAATACTGGCCTACCGTGATGAGTTGTCAAACAGTGAGGTGGCAAACCCTGAACATTTCCTTACTGCATATCAGAATCAACATGCACCTCAATACCTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       <  2:278254/150‑1 (MQ=255)
                             aTCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       <  2:1510111/150‑1 (MQ=255)
                             aTCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       <  2:1265579/150‑1 (MQ=255)
                               cTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACATCCCCGGATTTCACCGGGGCTGTTTCTAATTTCTTACTtata                                                                                                                     >  2:1571458/1‑150 (MQ=255)
                               cTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTtata                                                                                                                     <  2:553957/150‑1 (MQ=255)
                                tATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATAc                                                                                                                    >  1:1287847/1‑150 (MQ=255)
                                 aTGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACg                                                                                                                   >  2:1356322/1‑150 (MQ=255)
                                        aCTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTg                                                                                                            >  2:8230/1‑150 (MQ=255)
                                         cTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTgg                                                                                                           >  2:203458/1‑150 (MQ=255)
                                           ggCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCTGACTGGTg                                                                                                         >  2:526230/1‑150 (MQ=255)
                                               tACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       >  2:797685/1‑132 (MQ=255)
                                               tACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTcttactta                                                                                                                       <  1:797685/132‑1 (MQ=255)
                                               tACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAaca                                                                                                     <  1:765064/150‑1 (MQ=255)
                                               tACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAaca                                                                                                     >  1:1061253/1‑150 (MQ=255)
                                                aCAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAacac                                                                                                    <  2:939499/150‑1 (MQ=255)
                                                  aGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCa                                                                                                  <  1:8230/150‑1 (MQ=255)
                                                   gTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGACCACCaa                                                                                                 >  2:1714081/1‑150 (MQ=255)
                                                       tgaGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAACAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgc                                                                                             >  1:650221/1‑150 (MQ=255)
                                                          gTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcgcg                                                                                          >  1:405910/1‑150 (MQ=255)
                                                          gTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcgcg                                                                                          <  1:908679/150‑1 (MQ=255)
                                                          gTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcgcg                                                                                          <  1:810428/150‑1 (MQ=255)
                                                           ttGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACcgcgcgt                                                                                         <  1:1078068/150‑2 (MQ=255)
                                                             gTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGa                                                                                                        <  1:1080049/133‑1 (MQ=255)
                                                             gTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGa                                                                                                        >  2:1080049/1‑133 (MQ=255)
                                                             gTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGAcc                                                                                       >  2:261704/1‑150 (MQ=255)
                                                             gTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGAcc                                                                                       >  1:1237211/1‑150 (MQ=255)
                                                               cAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGTATCAACAAGCTCCTCAATAACTGAAACAGCCCCGGATGTCGGCGGGGCTGTGTCGCGTTTCTTACTCAAACGCCGACTGGTGCACACCAACCGAGCGccccg                                                                                     >  2:162527/1‑148 (MQ=255)
                                                               cAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAg                                                                                     >  1:1638138/1‑150 (MQ=255)
                                                               cAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAg                                                                                     >  2:1638109/1‑150 (MQ=255)
                                                                aaaCAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGa                                                                                    >  2:860339/1‑150 (MQ=255)
                                                                 aaCAGTGATGTCGCAAACACGGAAGATTTCCTTAGTGCATAGCTGAATCAAGAAGCACCTCAATTACTGAAACTGCCCCTGATTTCACCGGTGCTGTTTCGCATTGCTTACTTATACGCCGACTGGTGAACTCCTACCGCGCGACCAGAc                                                                                   <  1:251023/150‑1 (MQ=255)
                                                                  aCAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACg                                                                                  >  2:1241212/1‑150 (MQ=255)
                                                                       gATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCg                                                                             >  2:712255/1‑150 (MQ=255)
                                                                        aTGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCAGCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGAGAACACCAACCGCGCGACCAGACGGATCGt                                                                            <  2:394656/150‑1 (MQ=255)
                                                                        aTGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGt                                                                            <  1:1620791/150‑1 (MQ=255)
                                                                            ggCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAt                                                                        >  2:1495667/1‑150 (MQ=255)
                                                                            ggCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAt                                                                        >  1:493713/1‑150 (MQ=255)
                                                                            ggCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAt                                                                        >  1:500245/1‑150 (MQ=255)
                                                                             gCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAtt                                                                       >  1:1381261/1‑150 (MQ=255)
                                                                              cAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAACAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCAttt                                                                      >  1:552174/1‑150 (MQ=255)
                                                                                 aCCAGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCt                                                                   <  2:164264/150‑1 (MQ=255)
                                                                                  cccGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCtt                                                                  >  1:1252149/1‑150 (MQ=255)
                                                                                      gAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAAc                                                              <  1:1241212/150‑1 (MQ=255)
                                                                                       aaCATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACg                                                             <  1:1638109/150‑1 (MQ=255)
                                                                                         cATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCt                                                           >  1:1628297/1‑150 (MQ=255)
                                                                                         cATTTCCGTACTGCATATCAGAATCAACAAGAACCTCAATAACTGAAACAGCCCCGGATTACACCGGGCCTGTTTCGCAGTTCTTACTTATACGCAGACTGGTGCACACCAACCGCGCGACCAGCAGGATCGTCCTGTATCGTGAACGcg                                                           >  1:107917/1‑149 (MQ=255)
                                                                                           tttCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTc                                                         >  1:629316/1‑150 (MQ=255)
                                                                                              ccTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATc                                                      <  2:1671866/150‑1 (MQ=255)
                                                                                                   cTGCATATCAGAATCAACATGCACCTGAATAAGTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGATCACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCAtt                                                 <  2:247609/150‑1 (MQ=255)
                                                                                                   cTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCAtt                                                 >  1:88672/1‑150 (MQ=255)
                                                                                                     gCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCg                                               >  2:665939/1‑150 (MQ=255)
                                                                                                      cATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCCCCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCCACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGa                                              >  1:707283/1‑150 (MQ=255)
                                                                                                      cATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGa                                              >  1:404322/1‑150 (MQ=255)
                                                                                                       atatCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGAt                                             <  2:1061253/150‑1 (MQ=255)
                                                                                                        tatCAGAATCAACAATCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATc                                            <  2:1051727/150‑1 (MQ=255)
                                                                                                        tatCAGAATCAACAAGCACCACAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGa                                                                                >  2:1250180/1‑114 (MQ=255)
                                                                                                        tatCAGAATCAACAAGCACCACAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGa                                                                                <  1:1250180/114‑1 (MQ=255)
                                                                                                          tCAGAATCAACAAGCACCTCAACAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGc                                          <  2:650221/150‑1 (MQ=255)
                                                                                                            aGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTATCGCATTTCTTACTTATACGCCGACTGGTGACCACCAACCGCGCGCCCAGACGGATCGTCCATTTTATTGAACGCTTCATCCCAGTCGATCGCtt                                        >  1:136757/1‑150 (MQ=255)
                                                                                                             gAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCttt                                       <  2:405910/150‑1 (MQ=255)
                                                                                                              aaTCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTa                                      <  1:860339/150‑1 (MQ=255)
                                                                                                                tCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGc                                    <  1:1356322/150‑1 (MQ=255)
                                                                                                                 caacaaGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGCACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCg                                   >  2:73633/1‑150 (MQ=255)
                                                                                                                  aacaaGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCgg                                  >  1:665330/1‑150 (MQ=255)
                                                                                                                         aCCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACa                           <  2:1237211/150‑1 (MQ=255)
                                                                                                                            tCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGc                        <  1:665939/150‑1 (MQ=255)
                                                                                                                             cAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCg                       >  2:1075027/1‑150 (MQ=255)
                                                                                                                                  aCTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTTCTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGGTTTAGCGGTAGCACAAGCGACGGa                  >  2:198308/1‑150 (MQ=255)
                                                                                                                                   cTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGaa                 >  1:327179/1‑150 (MQ=255)
                                                                                                                                    tGAAACAGCCCCGGATTTCACCGGGGCTGCTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAg                <  1:674920/150‑1 (MQ=255)
                                                                                                                                    cgAAACAGCCCCGGTGAAATCCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAg                >  2:1007436/2‑150 (MQ=255)
                                                                                                                                    cgAAACAGCCCCGGTGAAATCCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAg                >  2:92984/2‑150 (MQ=255)
                                                                                                                                    cgAAACAGCCCCGGTGAAATCCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACAGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCCTCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAg                >  1:68281/2‑150 (MQ=255)
                                                                                                                                     gAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAgg               <  1:1495667/150‑1 (MQ=255)
                                                                                                                                     gAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAgg               <  2:88672/150‑1 (MQ=255)
                                                                                                                                     gAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCAATTCTTACTTATACGCCGACTGGTGAACACACACCGCGCGACCAGACGGATCGTCCAGTTTCTTGAACGCGTCATCCAAGTCGATCGCTTGAGCGGAAGCACAAGCGACGGAAgg               >  2:112798/1‑150 (MQ=255)
                                                                                                                                       aaCAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGAc             <  2:327179/150‑1 (MQ=255)
                                                                                                                                         cAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGCCGGAAGGACCg           >  2:1000293/1‑150 (MQ=255)
                                                                                                                                           gCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCCTCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGcc         >  1:300385/1‑150 (MQ=255)
                                                                                                                                            ccccGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGccc        <  2:1638138/150‑1 (MQ=255)
                                                                                                                                              ccGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCgg      <  1:468440/150‑1 (MQ=255)
                                                                                                                                              ccGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCgg      <  1:712255/150‑1 (MQ=255)
                                                                                                                                                ggATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGgca    >  1:1430430/1‑150 (MQ=255)
                                                                                                                                                  aTTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGgcacg  >  2:834/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGGAATTTAACAATAGTGGTGGGAAATTAATCTATGAAATACTGGCCTACAGTGATGAGTTGTCAAACAGTGATGTGGCAAACCCGGAACATTTCCTTACTGCATATCAGAATCAACAAGCACCTCAATAACTGAAACAGCCCCGGATTTCACCGGGGCTGTTTCGCATTTCTTACTTATACGCCGACTGGTGAACACCAACCGCGCGACCAGACGGATCGTCCATTTTCTTGAACGCTTCATCCCATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACG  >  NC_000913/697341‑697636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: