Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 741,043 C→G 6.3% intergenic (+118/+32) phr → / ← dtpD deoxyribodipyrimidine photolyase, FAD‑binding/dipeptide and tripeptide permease D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913741,0430CG 6.3% 363.1 / 10.2 109intergenic (+118/+32)phr/dtpDdeoxyribodipyrimidine photolyase, FAD‑binding/dipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base C (53/48);  new base G (2/5);  total (55/54)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 110 total aligned reads displayed.

CAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTGATCAAATAC  >  NC_000913/740894‑741185
                                                                                                                                                     |                                                                                                                                              
cAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTc                                                                                                                                                <  2:739987/150‑1 (MQ=255)
  gCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTGTCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCaa                                                                                                                                              >  2:1669382/1‑150 (MQ=255)
      aTGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGt                                                                                                                                          <  2:1327098/150‑1 (MQ=255)
       tGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTc                                                                                                                                         >  1:486687/1‑150 (MQ=255)
          ggcggcGGGGAAGGGGAAATAAAAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATTCGTAGGCCTGATAAGACGCGTCAAGCGTCGCa                                                                                                                                      <  1:1066971/150‑1 (MQ=255)
              gcgcGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAg                                                                                                                                  <  2:492284/150‑1 (MQ=255)
               cgcgGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTGTCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAgg                                                                                                                                 <  1:1669382/150‑1 (MQ=255)
                gcgGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                <  1:1062867/150‑1 (MQ=25)
                gcgGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGc                                                                                                                                <  1:796136/150‑1 (MQ=255)
                gaggAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGc                                                                                                                                <  2:412094/148‑1 (MQ=255)
                 cgGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCTGCCTACAAATTTGAGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc                                                                                                                               <  2:1033511/150‑2 (MQ=25)
                 cgGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATGCGACGCTTGACGCGTCTTATCAGGcc                                                                                                                               <  2:1473014/150‑2 (MQ=25)
                   gAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATCCAATAAATTGCAGGAAATACGTAGGCCTGATGCGACGCTTGACGCGTCTTATCAGGccta                                                                                                                             <  2:335646/150‑4 (MQ=25)
                    aaGGGGAAATAACAGACGCGTCAGGCAATCGAGACCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTAGCATCAGGCATCg                                                                                                                            >  2:1094717/1‑150 (MQ=255)
                     aGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCCGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCgg                                                                                                                           >  1:1081196/1‑150 (MQ=255)
                     aGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCTTCgg                                                                                                                           >  1:127856/1‑150 (MQ=255)
                       gggAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCTAGCCTACAAATTTGTGCTAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTg                                                                                                                         >  1:1427500/1‑150 (MQ=255)
                       gggAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTg                                                                                                                         >  1:646783/1‑150 (MQ=255)
                       gggAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTAGCATCAGGCATCGGTg                                                                                                                         >  2:662992/1‑150 (MQ=255)
                       gggAAATAACAGACGCGTCAGGCAAGCGAGCCCAGATGCCGGATGCGGCGTGATCGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTg                                                                                                                         <  2:1116746/150‑1 (MQ=255)
                        ggAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGc                                                                                                                        >  2:296455/1‑150 (MQ=255)
                        ggAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGc                                                                                                                        <  1:1071761/150‑1 (MQ=255)
                        ggAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGc                                                                                                                        >  2:1533863/1‑150 (MQ=255)
                         gAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCt                                                                                                                       <  2:1656311/150‑1 (MQ=255)
                             tAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGcc                                                                                                                   >  1:436873/1‑150 (MQ=255)
                               aCAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGCACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTa                                                                                                                 >  1:1629402/1‑150 (MQ=255)
                               aCAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGAGGCGTGAACGACTTATCCAGCCTACAAATTTCTGCAAATTAAAAAAAATGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGTCATCCGTGCTGTccaa                                                                                                                 >  1:593055/1‑148 (MQ=37)
                                 cgccgcgTCAGGCAATCGAGCCCAGCTGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTAtt                                                                                                               <  2:807897/147‑1 (MQ=255)
                                    cgcgTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAg                                                                                                            <  1:1642446/150‑1 (MQ=255)
                                     gcgTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGa                                                                                                           <  1:612048/150‑1 (MQ=255)
                                        tCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTc                                                                                                        <  1:1678238/150‑1 (MQ=255)
                                         cAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTcc                                                                                                       <  1:131857/150‑1 (MQ=255)
                                            gCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGTAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTccagc                                                                                                    <  1:177612/150‑1 (MQ=255)
                                             cAATCGAGCCCAGAGGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTccagcg                                                                                                   <  2:1644154/150‑1 (MQ=255)
                                              aaTCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTccagcgc                                                                                                  >  1:1402542/1‑150 (MQ=255)
                                               ttCGAGCCCAGCTGCCGGATTCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTGAATAACTTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTccagcgcc                                                                                                 <  1:1493031/149‑1 (MQ=255)
                                               ttCGAGCCAAGATGCATGATGCTGCGTGAACGCCTTATCCAGCCTACTAATTTGTTCAAATTCAATAATTTGCAGCATATACGTAGGCCTTATAAGACGCGTAAAGGGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTccagcgcc                                                                                                 <  2:593055/149‑1 (MQ=255)
                                               aTCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTccagcgcc                                                                                                 >  2:195683/1‑150 (MQ=255)
                                                tCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTccagcgcca                                                                                                <  2:600716/150‑1 (MQ=255)
                                                   aGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTccagcgccagcg                                                                                             >  1:12997/1‑150 (MQ=255)
                                                          aTGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGtt                                                                                      <  2:984237/150‑1 (MQ=255)
                                                          aTGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGtt                                                                                      >  2:944801/1‑150 (MQ=255)
                                                           tGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGttt                                                                                     >  1:946792/1‑150 (MQ=255)
                                                            gCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTc                                                                                    <  2:577613/150‑1 (MQ=255)
                                                            gCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTc                                                                                    >  2:1117119/1‑150 (MQ=255)
                                                            gCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGAGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCCTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTc                                                                                    <  2:1229406/150‑1 (MQ=255)
                                                             ccGGATGCGGCGTGATCGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCt                                                                                   <  2:38325/150‑1 (MQ=255)
                                                              cGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTg                                                                                  <  2:1032946/150‑1 (MQ=255)
                                                              cGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTg                                                                                  >  2:334784/1‑150 (MQ=255)
                                                                gATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGaa                                                                                >  1:1255399/1‑150 (MQ=255)
                                                                 aTGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAAt                                                                               >  2:1237851/1‑150 (MQ=255)
                                                                     ggcgTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAAAAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCa                                                                           >  1:576645/1‑150 (MQ=255)
                                                                      gcgTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAg                                                                          <  1:1533863/150‑1 (MQ=255)
                                                                       cgTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAgc                                                                         >  2:476429/1‑150 (MQ=255)
                                                                       cgTGAACGCCTTATCCAGCCTACAAATATGTGCAAATTCAATAAATTGCAGTAACTACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGAATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAgc                                                                         <  1:1106855/150‑1 (MQ=255)
                                                                        gTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAgcg                                                                        <  2:342833/150‑1 (MQ=255)
                                                                          gAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCCGCGCGCGGTTTCTGAATTTCAGCGcc                                                                      >  2:333526/1‑150 (MQ=255)
                                                                             cGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCAATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGa                                                                   >  2:187524/1‑150 (MQ=255)
                                                                              gCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGAt                                                                  >  1:196151/1‑150 (MQ=255)
                                                                              gCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGAt                                                                  >  2:412582/1‑150 (MQ=255)
                                                                                 ttATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACa                                                               >  2:639219/1‑150 (MQ=255)
                                                                                  tATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAg                                                              <  1:1055346/150‑1 (MQ=255)
                                                                                  tATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAg                                                              <  2:498247/150‑1 (MQ=255)
                                                                                  tATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAg                                                              <  2:1494279/150‑1 (MQ=255)
                                                                                     ccAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCa                                                           >  1:1009783/1‑150 (MQ=255)
                                                                                     ccAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCa                                                           <  2:436873/150‑1 (MQ=255)
                                                                                        gCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAat                                                        >  2:1299306/1‑150 (MQ=255)
                                                                                         ccTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCGTATTAAGACTCCAGCGCCAGCGCGCGGATTCTGAATTTCAGCGCCTGATACAGCCCAatc                                                       >  2:124275/1‑150 (MQ=255)
                                                                                         ccTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAatc                                                       >  2:1202665/1‑150 (MQ=255)
                                                                                          cTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAatca                                                      <  1:1540819/150‑1 (MQ=255)
                                                                                           tACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAatcat                                                     >  2:1049413/1‑150 (MQ=255)
                                                                                            aCAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAatcatc                                                    <  2:479009/150‑1 (MQ=255)
                                                                                                 tttGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTAc                                               <  2:12997/150‑1 (MQ=255)
                                                                                                  ttGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTAcc                                              <  1:1203169/150‑1 (MQ=255)
                                                                                                     tgCAAATTCAATAAATTGCCGTAAATACATAGGCCTGATAAGACGCGGCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCatg                                           <  2:1295525/150‑3 (MQ=255)
                                                                                                       cAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTcc                                         <  1:1117119/150‑1 (MQ=255)
                                                                                                       cAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTcc                                         >  1:423557/1‑150 (MQ=255)
                                                                                                        aaaTTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCCGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCt                                        >  2:717110/1‑150 (MQ=255)
                                                                                                          aTTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTac                                      >  2:696105/1‑150 (MQ=255)
                                                                                                           ttCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTaca                                     <  1:1143244/150‑1 (MQ=255)
                                                                                                             cAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTacaca                                   >  1:984953/1‑150 (MQ=255)
                                                                                                              aaTAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATTCAGCCAAATCATCTGTACCAGACGTacacac                                  >  2:86405/1‑150 (MQ=255)
                                                                                                              aaTAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTacacac                                  >  2:1333820/1‑150 (MQ=255)
                                                                                                               aTAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACg                                 >  2:1134057/1‑150 (MQ=255)
                                                                                                                       cAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTgcg                         >  1:1138605/1‑150 (MQ=255)
                                                                                                                        aGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTgcgc                        >  2:485818/1‑150 (MQ=255)
                                                                                                                              tACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAgg                  <  2:504021/150‑1 (MQ=255)
                                                                                                                               accgatgCCTGATGCGACGCTTGACGCGTCTTATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCCGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGt                 >  1:1382741/7‑150 (MQ=38)
                                                                                                                                   tggCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAACGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAAtt             <  2:1512818/149‑1 (MQ=255)
                                                                                                                                   aGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAAtt             <  1:696105/150‑1 (MQ=255)
                                                                                                                                    tgCCTGATGCGACGCTTGACGCGTCTTATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTCATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAAttt            >  2:1226954/2‑150 (MQ=255)
                                                                                                                                     gCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTg           >  1:341543/1‑150 (MQ=255)
                                                                                                                                       cTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTCCCAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTGAt         >  1:693629/1‑150 (MQ=255)
                                                                                                                                       cTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTGAt         >  2:921577/1‑150 (MQ=255)
                                                                                                                                        tGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCATCGACTTATACAGCCAAATCATCAGTACGAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTGATc        <  2:576645/150‑1 (MQ=255)
                                                                                                                                          aTAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTACAGCGCCTCATACAGCCAAAGCATCAGTACCAGTCCTACACACGCCAGTGCGCGCCAGGAAATAAGATCaa      >  2:1591433/1‑150 (MQ=255)
                                                                                                                                            aaGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTGATCAAAt    >  1:1423682/1‑150 (MQ=255)
                                                                                                                                            aaGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTGATCAAAt    <  1:476429/150‑1 (MQ=255)
                                                                                                                                              gACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTGATCAAATAc  >  1:1420668/1‑150 (MQ=255)
                                                                                                                                                  cgTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTac                                      >  2:411051/1‑110 (MQ=255)
                                                                                                                                                     |                                                                                                                                              
CAGCGTATGAGGCGGCGCGGAAGGGGAAATAACAGACGCGTCAGGCAATCGAGCCCAGATGCCGGATGCGGCGTGAACGCCTTATCCAGCCTACAAATTTGTGCAAATTCAATAAATTGCAGGAAATACGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATCGGTGCTGGCCTATTAAGACTCCAGCGCCAGCGCGCGGTTTCTGAATTTCAGCGCCTGATACAGCCAAATCATCAGTACCAGTCCTACACACGCCAGTGCGCCCCAGGTAATTTGATCAAATAC  >  NC_000913/740894‑741185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: