Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 779,438 T→G 5.3% R124R (CGT→CGG pal → peptidoglycan‑associated outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913779,4380TG 5.3% 327.5 / 6.9 113R124R (CGT→CGGpalpeptidoglycan‑associated outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (46/61);  new base G (4/2);  total (50/63)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

Only 100 of 118 total aligned reads displayed.

GGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACT  >  NC_000913/779291‑779586
                                                                                                                                                   |                                                                                                                                                    
ggACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAAcgtcg                                                                                                                                                    <  1:1053766/150‑1 (MQ=255)
ggACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAAcgtcg                                                                                                                                                    >  1:380247/1‑150 (MQ=255)
ggACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAAcgtcg                                                                                                                                                    >  2:25113/1‑150 (MQ=255)
  aCAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGCCGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTg                                                                                                                                                  <  2:1215144/150‑1 (MQ=255)
   cAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCTTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGc                                                                                                                                                 <  2:691839/150‑1 (MQ=255)
   cAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGc                                                                                                                                                 <  1:585894/150‑1 (MQ=255)
     aGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGa                                                                                                                                               <  2:131702/150‑1 (MQ=255)
     aGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGa                                                                                                                                               <  1:1416416/150‑1 (MQ=255)
      gTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTTGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGaa                                                                                                                                              <  1:1147808/150‑1 (MQ=255)
          gATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGcc                                                                                                                                          <  1:1356458/150‑1 (MQ=255)
           atatCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCAACGTAGAAGGTCACGCGGACGAACGTGGTACTCGGGAATACACCATCTCCCTGGGTGAACGTCGTGCGAACGCCg                                                                                                                                         >  2:1336959/1‑150 (MQ=255)
             atCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGtt                                                                                                                                       >  2:567088/1‑150 (MQ=255)
                cGTTCTGACTTCGCTCAACTGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGGCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAg                                                                                                                                    <  2:265505/150‑1 (MQ=255)
                 gTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGa                                                                                                                                   >  1:1273762/1‑150 (MQ=255)
                  ttCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGAt                                                                                                                                  >  1:122628/1‑150 (MQ=255)
                      gACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTAc                                                                                                                              >  2:544450/1‑150 (MQ=255)
                       aCTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTAcc                                                                                                                             <  1:189269/150‑1 (MQ=255)
                       aCTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTAcc                                                                                                                             <  2:1002979/150‑1 (MQ=255)
                        cTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCt                                                                                                                            >  2:1546997/1‑150 (MQ=255)
                         ttCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTg                                                                                                                           >  1:246075/1‑150 (MQ=255)
                           cGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCa                                                                                                                         >  1:272236/1‑150 (MQ=255)
                            gCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAg                                                                                                                        <  1:455777/150‑1 (MQ=255)
                             cTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAgg                                                                                                                       >  2:562253/1‑150 (MQ=255)
                             cTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAgg                                                                                                                       <  1:211976/150‑1 (MQ=255)
                              tCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAggg                                                                                                                      <  2:681957/150‑1 (MQ=255)
                               cAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGt                                                                                                                     <  2:1410556/150‑1 (MQ=255)
                                aaaTGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGGCTTACAAAGTCACCGTAGAAGGGCACGCGGACGAACGTGGTACTCCGGAATCCAACATCTCCCTGGGGGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTa                                                                                                                    <  2:1222769/150‑1 (MQ=255)
                                    gCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAgg                                                                                                                <  2:768293/150‑1 (MQ=255)
                                    gCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAgg                                                                                                                <  2:856836/150‑1 (MQ=255)
                                      tGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCg                                                                                                              <  2:380247/150‑1 (MQ=255)
                                       ggATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGt                                                                                                             <  2:458004/150‑1 (MQ=255)
                                        gATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGtt                                                                                                            <  2:627933/150‑1 (MQ=255)
                                          tGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTc                                                                                                          <  1:507264/150‑1 (MQ=255)
                                            cacaTGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTg                                                                                                        >  1:493325/1‑150 (MQ=255)
                                                    aaCTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAg                                                                                                <  2:272236/150‑1 (MQ=255)
                                                    aaCTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAg                                                                                                <  2:1622256/150‑1 (MQ=255)
                                                    aaCTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAg                                                                                                <  1:332126/150‑1 (MQ=255)
                                                           tGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCa                                                                                         <  1:20253/150‑1 (MQ=255)
                                                             cggaGCAACCCGGCTTACAAATGCCACGTAGCAGGTCACGCGGACGAACGTGGTACACCGGAAAACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATc                                                                                       <  2:1519955/147‑1 (MQ=255)
                                                             cGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATc                                                                                       >  2:851045/1‑150 (MQ=255)
                                                             cGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACCTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATc                                                                                       <  2:38451/150‑1 (MQ=255)
                                                              gTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCg                                                                                      <  1:80549/150‑1 (MQ=255)
                                                              gTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGATAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCACAACTCCATCg                                                                                      >  1:1149654/1‑150 (MQ=255)
                                                                 gCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGttt                                                                                   >  1:354688/1‑150 (MQ=255)
                                                                 gCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCGGCAGGGTAAAGGCGTTACGGCAGACCCGATCTCCATCGttt                                                                                   >  2:1631298/1‑150 (MQ=255)
                                                                  cAACCCGTCTTCCAAAGTCACCGTAGAAGGGCACGCTGACGAACGTGGTCCTCCGGAAGACAACATCTCCCTGGGGGAACGGCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGAGCTCCATCGTTTc                                                                                  <  1:98472/150‑1 (MQ=255)
                                                                  cAACCCGTCTAACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTc                                                                                  <  1:331450/150‑1 (MQ=255)
                                                                     cccGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGCTCTCCATCGTTTCTTa                                                                               >  2:1204220/1‑150 (MQ=255)
                                                                      ccGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTAc                                                                              <  1:254271/150‑1 (MQ=255)
                                                                      ccGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTAc                                                                              <  1:25113/150‑1 (MQ=255)
                                                                        gTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACgg                                                                            <  1:948150/150‑1 (MQ=255)
                                                                          cTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTGCATCGTTTCTTACGGTa                                                                          >  1:1579094/1‑150 (MQ=255)
                                                                          cTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTa                                                                          <  1:962486/150‑1 (MQ=255)
                                                                           ttACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTaa                                                                         <  2:1064943/150‑1 (MQ=255)
                                                                           ttACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTaa                                                                         <  1:851045/150‑1 (MQ=255)
                                                                           ttACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTCCTTACGGTaa                                                                         >  1:4595/1‑150 (MQ=255)
                                                                            tACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACTCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTaaa                                                                        >  2:1185451/1‑150 (MQ=255)
                                                                            tACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTaaa                                                                        >  2:1278718/1‑150 (MQ=255)
                                                                                aaGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGaaa                                                                    >  2:365037/1‑150 (MQ=255)
                                                                                   tCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAAc                                                                 <  2:609344/150‑1 (MQ=255)
                                                                                    cACCGTAGAAGGTCACGCGGACGAACGAGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCGATCGTTTCTTACGGTAAAGAAAAAcc                                                                >  1:501139/1‑150 (MQ=255)
                                                                                      ccGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTa                                                                               <  1:1137709/133‑1 (MQ=255)
                                                                                      ccGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTg                                                              >  1:238660/1‑150 (MQ=255)
                                                                                       cGTAGAAGGTCACGGGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTTCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGc                                                             <  1:1546997/150‑1 (MQ=255)
                                                                                       cGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGc                                                             <  2:354688/150‑1 (MQ=255)
                                                                                        gTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGTGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCa                                                            <  1:1502591/150‑1 (MQ=255)
                                                                                             agttCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACt                                                       <  2:328437/147‑1 (MQ=255)
                                                                                             aGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACt                                                       >  1:765469/1‑150 (MQ=255)
                                                                                             aGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACt                                                       >  1:925950/1‑150 (MQ=255)
                                                                                               gTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTgg                                                     <  1:567088/150‑1 (MQ=255)
                                                                                               gTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTgg                                                     >  2:921054/1‑150 (MQ=255)
                                                                                                       gACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGAc                                             <  2:696807/150‑1 (MQ=255)
                                                                                                        aCGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACg                                            <  2:1216544/150‑1 (MQ=255)
                                                                                                            aCGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGc                                        <  2:417267/150‑1 (MQ=255)
                                                                                                              gTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCTGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCgg                                      <  1:245474/150‑1 (MQ=255)
                                                                                                              gTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCgg                                      >  2:962359/1‑150 (MQ=255)
                                                                                                               ggttACTCCGGAATACAACATCTCACTGGGTGAACGTCGTGCGAACGCCGGTAAGATGTACCTGCAGGGAAAAGGCGGTTCTGCAGACCTGATCTCCATCGTTTCTTACTGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGc                                     <  1:674026/147‑1 (MQ=255)
                                                                                                                    cTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGGTTCTGCAGACCAGATCTCCATCGTTTCGTACGGTAAAGAAAAACCTGCAGGACTGGGTCATGACGAAGCGGCATACt                                >  2:102766/1‑150 (MQ=255)
                                                                                                                      ccGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTcc                              >  1:1652275/1‑150 (MQ=255)
                                                                                                                      ccGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTcc                              <  2:765469/150‑1 (MQ=255)
                                                                                                                      ccGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTcc                              <  1:647479/150‑1 (MQ=255)
                                                                                                                      ccGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGATTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTcc                              <  1:641278/150‑1 (MQ=255)
                                                                                                                       tggAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCa                             <  2:1406229/149‑1 (MQ=255)
                                                                                                                       cGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCa                             >  1:1101664/1‑150 (MQ=255)
                                                                                                                       cGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCa                             <  1:1345303/150‑1 (MQ=255)
                                                                                                                           aTACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCaaaaa                         >  2:1154118/1‑150 (MQ=255)
                                                                                                                           aTACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCaaaaa                         >  1:1682242/1‑150 (MQ=255)
                                                                                                                            tACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATAGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAAc                        >  1:1572991/1‑150 (MQ=255)
                                                                                                                             acaacaTCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAAcc                       >  2:528241/1‑150 (MQ=255)
                                                                                                                              caacaTCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACcg                      <  1:921054/150‑1 (MQ=255)
                                                                                                                                 caTCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGTGGAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACcgtcg                   <  1:1336959/150‑1 (MQ=255)
                                                                                                                                 caTCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACcgtcg                   >  2:1674341/1‑150 (MQ=255)
                                                                                                                                       ccTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGt             >  1:361253/1‑150 (MQ=255)
                                                                                                                                          gggTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACt          >  2:1536747/1‑150 (MQ=255)
                                                                                                                                           tcttaaCGGCGTTCGCACGACGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTg         >  2:954620/5‑150 (MQ=255)
                                                                                                                                           tcttaaCGGCGTTCGCACGACGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTg         >  1:1083702/5‑150 (MQ=255)
                                                                                                                                           ggTGAACGTCGTGCGACCGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAACCCGTAGTGCGGTACTg         >  2:314790/1‑150 (MQ=255)
                                                                                                                                            cttaaCGGCGTTCGCACGACGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTgg        >  1:1516210/4‑150 (MQ=255)
                                                                                                                                             ttaaCGGCGTTCGCACGACGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGt       >  1:1667178/3‑150 (MQ=255)
                                                                                                                                                  gtcgtGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCTTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACt  <  1:974333/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACT  >  NC_000913/779291‑779586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: