Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 808,430 T→A 5.1% L276F (TTA→TTT bioA ← 7,8‑diaminopelargonic acid synthase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913808,4300TA 5.1% 225.4 / 2.5 78L276F (TTA→TTTbioA7,8‑diaminopelargonic acid synthase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (43/31);  new base A (2/2);  total (45/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

TCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCA  >  NC_000913/808282‑808579
                                                                                                                                                    |                                                                                                                                                      
tCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTa                                                                                                                                                       <  2:767779/150‑1 (MQ=255)
 cGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTaa                                                                                                                                                      >  2:821863/1‑150 (MQ=255)
  gCCAGGCTGCCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAACGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGGGGTGAGTGTGGCGGAAAGGGGCATTGTGCCGCCGGTTAAg                                                                                                                                                     <  1:1084512/150‑1 (MQ=255)
   ccAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAgg                                                                                                                                                    >  2:791399/1‑150 (MQ=255)
   ccAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAgg                                                                                                                                                    <  2:1618854/150‑1 (MQ=255)
       gCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGGGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCttt                                                                                                                                                <  1:524627/150‑1 (MQ=255)
        cTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTa                                                                                                                                               >  2:380536/1‑150 (MQ=255)
          ggCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTAcc                                                                                                                                             >  2:296796/1‑150 (MQ=255)
          ggCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTAcc                                                                                                                                             >  2:848676/1‑150 (MQ=255)
               ttGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACAGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGc                                                                                                                                       <  1:234283/150‑1 (MQ=255)
                 gctgcCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCTCGGTAAAGCCTTAACCggcggcac                                                                                                                                    <  1:392491/150‑8 (MQ=255)
                      cgcgCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAAt                                                                                                                                >  2:1157054/1‑150 (MQ=255)
                      cgcgCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTATGAACCCTCGCGCGTGTGGAGTGGGGCGGAAAGGGTAATGGTGCCCCCGGATAAGGATATACCGCGGCACAAAAt                                                                                                                                >  1:5695/1‑150 (MQ=255)
                       gcgcAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCTCGGTAAAGCCTTAACCGGCGGCACaatgac                                                                                                                               <  1:541660/150‑5 (MQ=38)
                          cAGGCCAGCGGATTGCCCATACACGTTGGCCCATGCATACAGCAACCGGCTTCACCGTTACTGATTGTTTCTGCAACCTCGCGCGTGTTGACTGTGTCGGAAATGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTcc                                                                                                                            <  1:296796/150‑1 (MQ=255)
                           aGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCg                                                                                                                           >  2:1340741/1‑150 (MQ=255)
                            ggCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCgg                                                                                                                          <  1:380536/150‑1 (MQ=255)
                            ggCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCgg                                                                                                                          <  2:281253/150‑1 (MQ=255)
                              ccAGCGGATTGCCCATAAAAGTTGGCCCATGCATACAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGgcg                                                                                                                        <  1:1248281/150‑1 (MQ=255)
                              ccAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTACAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGgcg                                                                                                                        >  1:1715208/1‑150 (MQ=255)
                                  cGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGAt                                                                                                                    >  2:942817/1‑150 (MQ=255)
                                       tGCCCATAAAAGTTGGCTCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTg                                                                                                               <  2:175691/150‑1 (MQ=255)
                                        gCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGATAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGc                                                                                                              >  1:621362/1‑150 (MQ=255)
                                         cccATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCa                                                                                                             >  2:1213071/1‑150 (MQ=255)
                                         cccATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCa                                                                                                             <  1:420487/150‑1 (MQ=255)
                                               aaaGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAATATGTCCGGCGCGATTTCTGCATGTTca                                                                                                       >  2:138423/1‑150 (MQ=255)
                                               aaaGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTca                                                                                                       >  1:686359/1‑150 (MQ=255)
                                                  gTTGGCCCATGTATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAg                                                                                                    <  1:175501/150‑1 (MQ=255)
                                                      gCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCaa                                                                                                >  2:980299/1‑150 (MQ=255)
                                                          aTGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAg                                                                                            <  1:821863/150‑1 (MQ=255)
                                                            gCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGtt                                                                                          >  1:457368/1‑150 (MQ=255)
                                                               tAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTcc                                                                                       >  1:1259468/1‑150 (MQ=255)
                                                                 aaGCAACCGGCTTCACCGTTACTGATGGTTTCAGCACCCTCGCGCGTGGTGAGTGTGGCGGAAAGGGGCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCg                                                                                     <  2:817260/150‑1 (MQ=255)
                                                                      aCCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACg                                                                                <  1:115491/150‑1 (MQ=255)
                                                                        cGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGAc                                                                              <  1:359228/150‑1 (MQ=255)
                                                                         ggCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGCCAAACAGTTTCCCGGTACGAcc                                                                             >  2:1247911/1‑150 (MQ=255)
                                                                            ttCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCaaa                                                                          >  2:1488675/1‑150 (MQ=255)
                                                                             tCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAAt                                                                         >  2:460599/1‑150 (MQ=255)
                                                                             tCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAAt                                                                         >  2:466949/1‑150 (MQ=255)
                                                                              cACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGGCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATc                                                                        <  1:1045762/150‑1 (MQ=255)
                                                                                         aTGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGAtc                                                             <  2:1661993/150‑1 (MQ=255)
                                                                                         aTGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGAtc                                                             >  2:909957/1‑150 (MQ=255)
                                                                                          tGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGAtct                                                            <  1:848676/150‑1 (MQ=255)
                                                                                           ggTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAACATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGAtctg                                                           >  2:1037740/1‑149 (MQ=255)
                                                                                            gTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCtcg                                                          >  2:1375411/1‑150 (MQ=255)
                                                                                            gTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCtcg                                                          >  1:694673/1‑150 (MQ=255)
                                                                                             tttCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCtcgt                                                         >  2:655717/1‑150 (MQ=255)
                                                                                             tttCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCtcgt                                                         <  1:1157054/150‑1 (MQ=255)
                                                                                              ttCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATAGTCCCGCCGGTTAAGGCATTACCGAGGCACAAATTGTCCGGCGCGATTTCTGCATGTTAACAGGCAAACACTTTCCCGGTACGACCAAATGCCGTGGCGATCtcgtc                                                        >  2:916747/1‑150 (MQ=255)
                                                                                               tCTGCACCCTCGCGCGTGGGGAGTGTGGCGGAAAGGGTCATAGTGCCGCCGGTTAAGGCTTTACCGAAGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCtcgtcg                                                       <  2:229878/150‑1 (MQ=255)
                                                                                                cTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCgg                                                      <  1:1409596/150‑1 (MQ=255)
                                                                                                 tGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGc                                                     >  2:1214960/1‑150 (MQ=255)
                                                                                                 tGCAACCTCGCGCGTGGTGAGTGTAGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGc                                                     >  1:745910/1‑150 (MQ=255)
                                                                                                 tGCAACCTCGCGCGTGGGGAGTGTGGCGGAAAGGGTCATAGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGc                                                     <  2:180189/150‑1 (MQ=255)
                                                                                                 tGCAACCTCGCGCGGGGGGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGc                                                     <  2:1464963/150‑1 (MQ=255)
                                                                                                  gCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCa                                                    >  2:1595393/1‑150 (MQ=255)
                                                                                                  gCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCa                                                    >  2:154001/1‑150 (MQ=255)
                                                                                                  gCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCa                                                    >  2:92599/1‑150 (MQ=255)
                                                                                                  gCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCaa                                                   >  2:1003801/1‑150 (MQ=255)
                                                                                                      ccTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCa                                                >  1:19098/1‑150 (MQ=255)
                                                                                                      ccTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCa                                                <  2:1263463/150‑1 (MQ=255)
                                                                                                       cTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAg                                               >  1:1066608/1‑150 (MQ=255)
                                                                                                         cgcgcgTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCa                                             >  1:658142/1‑150 (MQ=255)
                                                                                                           cgcgTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAg                                           <  1:454692/150‑1 (MQ=255)
                                                                                                                           cGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGCCCAAATCCAGTGGCGAGCTCGTCGGCCATCAGCAAGATACCTTCGCGATCGc                           >  2:1009069/1‑150 (MQ=255)
                                                                                                                           cGGAAAGGGTCATTGAGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGc                           >  2:292174/1‑150 (MQ=255)
                                                                                                                            ggAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGCCCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCa                          >  1:972901/1‑150 (MQ=255)
                                                                                                                                 gggTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATAttt                     >  1:787133/1‑150 (MQ=255)
                                                                                                                                   gTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCACTCAGCAAGATACCTTCGCGATCGCATATTTTg                   >  1:725705/1‑150 (MQ=255)
                                                                                                                                    tCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGc                  <  1:1133253/150‑1 (MQ=255)
                                                                                                                                     ttttGTGCCTCGGTAAAGCCTTAACCGGCGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCg                 >  2:1669725/3‑150 (MQ=38)
                                                                                                                                     cATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTTACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGACAATCAGCAAGATACCGTCGCGATCGCATATTTTGCg                 >  2:310924/1‑150 (MQ=255)
                                                                                                                                     cATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCg                 <  1:791399/150‑1 (MQ=255)
                                                                                                                                     cATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCg                 <  1:1340741/150‑1 (MQ=255)
                                                                                                                                        tgtgCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGAt              <  1:92599/150‑1 (MQ=255)
                                                                                                                                          gtgcctcgGTAAAGCCTTAACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTc            >  2:1353677/7‑150 (MQ=255)
                                                                                                                                           gccgccGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCg           >  2:403213/1‑150 (MQ=255)
                                                                                                                                                cgGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTa      <  1:1488675/150‑1 (MQ=255)
                                                                                                                                                    tAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCa  >  2:1665326/1‑150 (MQ=255)
                                                                                                                                                    tAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCa  <  1:942817/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TCGCCAGGCTGGCGTTTGCTGCCGCGCAGGCCAGCGGATTGCCCATAAAAGTTGGCCCATGCATAAAGCAACCGGCTTCACCGTTACTGATGGTTTCTGCAACCTCGCGCGTGGTGAGTGTGGCGGAAAGGGTCATTGTGCCGCCGGTTAAGGCTTTACCGAGGCACAAAATGTCCGGCGCGATTTCTGCATGTTCACAGGCAAACAGTTTCCCGGTACGACCAAATCCAGTGGCGATCTCGTCGGCAATCAGCAAGATACCTTCGCGATCGCATATTTTGCGGATTCGTTTTAACCA  >  NC_000913/808282‑808579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: