Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 830,666 A→T 6.4% intergenic (‑23/‑206) ybiH ← / → rhlE DUF1956 domain‑containing tetR family putative transcriptional regulator/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913830,6660AT 6.4% 295.9 / 6.9 103intergenic (‑23/‑206)ybiH/rhlEDUF1956 domain‑containing tetR family putative transcriptional regulator/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (50/46);  new base T (2/5);  total (52/51)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CTGGGCGGCTATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCT  >  NC_000913/830524‑830815
                                                                                                                                              |                                                                                                                                                     
cTGGGCGGCTATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGACTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAAtgattga                                                                                                                                                <  1:1469775/150‑1 (MQ=255)
     cggcTATCTCTCGAGTGCTGGCGTTCATACCAAATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAg                                                                                                                                           <  2:265426/150‑1 (MQ=255)
     cggcTATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAg                                                                                                                                           <  1:1388094/150‑1 (MQ=255)
       gcTATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATACATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGAt                                                                                                                                         >  1:1451980/1‑150 (MQ=255)
        cTATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAATCATTTGATTAAGaat                                                                                                                                        <  2:768316/150‑3 (MQ=255)
        cTATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAATCATTTGATTAAGaat                                                                                                                                        <  2:93193/150‑3 (MQ=255)
         tATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAAtgattg                                                                                                                                                 >  1:94927/1‑140 (MQ=255)
         tATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAAtgattg                                                                                                                                                 <  2:94927/140‑1 (MQ=255)
          aTCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTTTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAATCATTTGATTAAGaatgg                                                                                                                                      <  1:1421385/150‑5 (MQ=255)
           tctcGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCATCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATg                                                                                                                                     <  1:439579/150‑1 (MQ=255)
           tctcGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATg                                                                                                                                     >  2:1562291/1‑150 (MQ=255)
            ctcGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGa                                                                                                                                    >  2:1025221/1‑150 (MQ=255)
                     ggtggCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCAACCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAgg                                                                                                                           >  1:1229578/1‑150 (MQ=255)
                       tggAGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCTATCAGCTGTTTTTTCGCCTTTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAgggg                                                                                                                         <  2:206639/150‑1 (MQ=255)
                         gCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAg                                                                                                                       >  2:88624/1‑150 (MQ=255)
                         gCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAg                                                                                                                       >  2:1286210/1‑150 (MQ=255)
                          cGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGt                                                                                                                      >  2:125947/1‑150 (MQ=255)
                          cGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGt                                                                                                                      >  1:1648023/1‑150 (MQ=255)
                              cATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTc                                                                                                                  >  2:1316743/1‑150 (MQ=255)
                               aTTCCATATTCACCCCACTGCGCCCGGGCGGCAGCAATCAGCGGTTTTTTCGCCTGTTCACCCTTGCTTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTcc                                                                                                                 <  2:29408/150‑1 (MQ=255)
                               aTTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTcc                                                                                                                 <  1:1030003/150‑1 (MQ=255)
                                ttCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCa                                                                                                                >  2:1022620/1‑150 (MQ=255)
                                ttCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCa                                                                                                                <  1:427835/150‑1 (MQ=255)
                                 tCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAg                                                                                                               >  1:75759/1‑150 (MQ=255)
                                  ccATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCAACGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGAATGATTAAGATTATGACTCCATAGTGGAGTTTTCCAGt                                                                                                              >  1:70842/1‑150 (MQ=255)
                                     tatTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATg                                                                                                           <  2:76764/150‑1 (MQ=255)
                                     tatTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATg                                                                                                           <  1:422318/150‑1 (MQ=255)
                                     tatTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCAGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATg                                                                                                           <  2:1566064/150‑1 (MQ=255)
                                      atTCACCAATCTGCGCGAGTGCGGCAGCAATCAGCTGTTTTTTCGACTGTTCAACCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGTTTATGACTCCATAGGGGAGTTGTCCAGTATgg                                                                                                          <  1:1583248/150‑1 (MQ=255)
                                       ttCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGc                                                                                                         <  2:1680647/150‑1 (MQ=255)
                                        tCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCt                                                                                                        <  1:445477/150‑1 (MQ=255)
                                         cACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTTTGACTCCATAGGGGAGTTGTCCAGTATGGCTa                                                                                                       <  2:300251/150‑1 (MQ=255)
                                         cACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTa                                                                                                       >  2:199135/1‑150 (MQ=255)
                                          aCCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTaa                                                                                                      >  2:1011492/1‑150 (MQ=255)
                                              tccTGCGCCAGTCCGGCAGCAATCAGCTGTTTGATCGCCTGTGCACCCTTGATTGTCCTGGCAGGATTCTTCATCGCACTGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAAt                                                                                                  <  1:262744/148‑1 (MQ=255)
                                               aCTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAAtt                                                                                                 <  2:1164949/150‑1 (MQ=255)
                                                 tGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTGCACCCTTGATTGGCATGGCAGGATTATTCATCGCACAGTCCATTGTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAAtttt                                                                                               <  2:1502675/150‑1 (MQ=255)
                                                  gcgcCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATTGGGGAGTTGTCCAGTATGGCTAAGAATTTTa                                                                                              >  1:681462/1‑150 (MQ=255)
                                                  gcgcCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGAGTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATTGCTAAGAATTTTa                                                                                              >  1:314910/1‑150 (MQ=255)
                                                   cgcCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAg                                                                                             <  1:655192/150‑1 (MQ=255)
                                                    gcCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGc                                                                                            >  2:747636/1‑150 (MQ=255)
                                                     ccAGTGCGGCAGCAATCAGCTGTTTTTTCGCGTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTTAGATTATGACTCCATAGGGGAGTTGTCCAGTATTGCTAAGAATTTTAGCa                                                                                           >  2:800905/1‑150 (MQ=255)
                                                     ccAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATGGCACAGTCCATTCTTTATCAAATGATTGATTAAGATTATTACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTATCa                                                                                           >  2:800904/1‑150 (MQ=255)
                                                       aGTGCGGCAGCAATCAGCGGTGTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGAGTATTCATCGCACAGTCCATTCTTTATCAAATGATTGATTAAGATTATGACTCCATAGTGGAGTTGTCCTGTATGGCTCAGAATTTTAGCAAc                                                                                         <  2:716967/150‑1 (MQ=255)
                                                         tGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGc                                                                                       <  2:527394/150‑1 (MQ=255)
                                                         agctgcagcaAGCAGCTGTTTTTTCGACGTTTCACGCTTGATTGTCATGGCCGGAGTATTCATCGCACAGTCCAATCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGc                                                                                       <  2:61877/146‑1 (MQ=255)
                                                          gCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGcc                                                                                      <  1:1025221/150‑1 (MQ=255)
                                                          gCGGCAGCAATCAGCTGTTTATTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGcc                                                                                      <  1:320754/150‑1 (MQ=255)
                                                            ggCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCGTCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAg                                                                                    >  1:1545409/1‑150 (MQ=255)
                                                            ggCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAg                                                                                    <  2:1648023/150‑1 (MQ=255)
                                                              cagcaATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTc                                                                                  <  1:914756/150‑1 (MQ=255)
                                                              cagcaATCAGCTGTTTTTACGCCTGTTCCCCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCCTAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTc                                                                                  <  1:1107572/150‑1 (MQ=255)
                                                                   aTCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAgg                                                                             <  2:82513/150‑1 (MQ=255)
                                                                   aTCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAgg                                                                             >  2:1343876/1‑150 (MQ=255)
                                                                   aTCAGCTGTTTTTTCGCCGGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGTTTATGACTCCAGAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAgg                                                                             >  2:1389678/1‑150 (MQ=255)
                                                                       gCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTTTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACATGGATa                                                                         >  1:1433039/1‑150 (MQ=255)
                                                                          gTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAAtt                                                                      <  1:411339/150‑1 (MQ=255)
                                                                            tttttCGCCTGTTCACCCTTTATTGTCATGGCAGTATAATTCATCGCAAAGTCCAATCTAAATCAATTGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTa                                                                    <  1:1557479/150‑1 (MQ=255)
                                                                            tttttCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTa                                                                    >  2:1351554/1‑150 (MQ=255)
                                                                                tCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATgcg                                                                <  2:1229578/150‑1 (MQ=255)
                                                                                 cGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATgcgc                                                               <  1:1463977/150‑1 (MQ=255)
                                                                                   ccTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTg                                                             >  2:352635/1‑150 (MQ=255)
                                                                                    cTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGc                                                            >  2:907446/1‑150 (MQ=255)
                                                                                        tCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTca                                                        <  2:66057/150‑1 (MQ=255)
                                                                                           cccTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACaa                                                     >  1:355142/1‑150 (MQ=255)
                                                                                             cTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACaaaa                                                   >  2:1250215/1‑150 (MQ=255)
                                                                                             cTTGATTGTCATGGCAGGATTATTCATCGCACAGTCAATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGAGAATTTATGCGCTGCGTCACataa                                                   >  2:1353393/1‑150 (MQ=255)
                                                                                                  ttGTCATGGCAGGATTATTCATCGCTCAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGc                                              <  1:199135/150‑1 (MQ=255)
                                                                                                  ttGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGc                                              <  1:88624/150‑1 (MQ=255)
                                                                                                   tGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGACAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCt                                             >  1:47156/1‑150 (MQ=255)
                                                                                                    gTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTa                                            >  2:958064/1‑150 (MQ=255)
                                                                                                        tGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACt                                        <  1:929542/150‑1 (MQ=255)
                                                                                                        tGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCAAAGGGATAATTTATGAGCTGCGTCACAAAAACTGCTACACt                                        >  1:252953/1‑150 (MQ=255)
                                                                                                         ggCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTc                                       <  1:1011492/150‑1 (MQ=255)
                                                                                                          gCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTcc                                      >  2:1539472/1‑150 (MQ=255)
                                                                                                             ggATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCt                                   >  2:453972/1‑150 (MQ=255)
                                                                                                                  attCATCGGACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACATGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTc                              <  2:75759/150‑1 (MQ=255)
                                                                                                                     cATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATg                           >  2:787308/1‑150 (MQ=255)
                                                                                                                     cATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATg                           >  2:1296561/1‑150 (MQ=255)
                                                                                                                     cATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATg                           <  1:15695/150‑1 (MQ=255)
                                                                                                                     cATCGCACAGTCCATTCTTAATCAAATGATTGAATAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATg                           <  1:97943/150‑1 (MQ=255)
                                                                                                                     acgcGCACAGACCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGGCACAAAAACTGCTACACTCCGCTCCCTCATg                           <  1:877474/147‑1 (MQ=255)
                                                                                                                             gtcataatCTTAATCAATCATTTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATtgtg                   >  1:61810/8‑150 (MQ=255)
                                                                                                                             aGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATtgtg                   >  2:709720/1‑150 (MQ=255)
                                                                                                                              tcataatCTTAATCAATCATTTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTgg                  <  2:61810/144‑1 (MQ=255)
                                                                                                                              gTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTgg                  >  2:1399105/1‑150 (MQ=255)
                                                                                                                              gTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTgg                  <  2:1614592/150‑1 (MQ=255)
                                                                                                                               tCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGt                 <  1:1359622/150‑1 (MQ=255)
                                                                                                                                ccATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGtt                >  2:423773/1‑150 (MQ=255)
                                                                                                                                ccATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGAGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTgggt                >  2:924570/1‑148 (MQ=255)
                                                                                                                                     cTTAATCAATCATTTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGt           >  1:742173/1‑150 (MQ=255)
                                                                                                                                     cTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGt           >  2:1372980/1‑150 (MQ=255)
                                                                                                                                     cTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGt           >  2:1394693/1‑150 (MQ=255)
                                                                                                                                      taaaTCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCAAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGta          >  1:827967/3‑149 (MQ=255)
                                                                                                                                       tAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCa         <  1:352635/150‑1 (MQ=255)
                                                                                                                                         aTCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCAtt       >  2:1377329/1‑150 (MQ=255)
                                                                                                                                          tCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCAttt      <  1:207325/150‑1 (MQ=255)
                                                                                                                                             aaTGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTcc   <  1:1296561/150‑1 (MQ=255)
                                                                                                                                              aTGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCAGAGCGATAATATATGCGCTGCGTCACAAAAAATGCTACACGCCGCTACCTCAAGACATTGTGGTTTTTGTCATTTTcca  >  2:1291819/1‑149 (MQ=255)
                                                                                                                                              aTGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCt  >  1:864546/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
CTGGGCGGCTATCTCGCGAGTGGTGGCGTTCATTCCATATTCACCAAACTGCGCCAGTGCGGCAGCAATCAGCTGTTTTTTCGCCTGTTCACCCTTGATTGTCATGGCAGGATTATTCATCGCACAGTCCATTCTTAATCAAATGATTGATTAAGATTATGACTCCATAGGGGAGTTGTCCAGTATGGCTAAGAATTTTAGCAACGCCAGTCACAGGGATAATTTATGCGCTGCGTCACAAAAACTGCTACACTCCGCTCCCTCATGACATTGTGGTTTTTGTCATTTTCCT  >  NC_000913/830524‑830815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: