Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 892,459 G→C 7.4% D165H (GAC→CAC)  rimK → ribosomal protein S6 modification protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913892,4590GC 7.4% 305.9 / 11.8 94D165H (GAC→CAC) rimKribosomal protein S6 modification protein
Reads supporting (aligned to +/- strand):  ref base G (35/52);  new base C (3/4);  total (38/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTG  >  NC_000913/892313‑892608
                                                                                                                                                   |                                                                                                                                                     
tCACGGGCATTGCGCATTCGCCGGATGATACGAGCGATTTAATCGACATGGTCGGTGGTGCGCCTCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACg                                                                                                                                                    <  1:1302930/150‑1 (MQ=255)
 cACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCCGGCGGCGGAAAGCGTGATTGACGc                                                                                                                                                   >  2:34439/1‑150 (MQ=255)
 cACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGc                                                                                                                                                   >  1:1343619/1‑150 (MQ=255)
   cGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCtt                                                                                                                                                 <  2:549177/150‑1 (MQ=255)
     ggCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTc                                                                                                                                               <  1:1402773/150‑1 (MQ=255)
      gCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCTTTcc                                                                                                                                              <  1:557680/150‑1 (MQ=255)
       cATTGCTCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCTTTCcg                                                                                                                                             <  2:1400175/150‑1 (MQ=255)
         ttGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTCAATCACGCTTTCcgcc                                                                                                                                           <  1:1210364/150‑2 (MQ=255)
         ttGCGCATTCGCCGGATGATACCAGCGATTTAAGCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTGTCcgcg                                                                                                                                           <  1:916400/150‑1 (MQ=255)
           gcgccgtCGCCGGATGCTTCCAGCGATTTAATCGACATGGGCGGTGGCGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCGGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGt                                                                                                                                         <  1:1525450/144‑1 (MQ=255)
           gcgcATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGt                                                                                                                                         >  2:1663438/1‑150 (MQ=255)
             gcATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTTCAGACCGCGGAAAGCGTCAATCACGCTTTCcgccgcc                                                                                                                                        <  2:379424/150‑5 (MQ=38)
                 tCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAc                                                                                                                                   <  2:540145/150‑1 (MQ=255)
                  cGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                  <  1:1185964/150‑1 (MQ=255)
                  cGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                  >  1:1200579/1‑150 (MQ=255)
                  cGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGGGCGCCGCTGGTGGTCATGGTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcg                                                                                                                                  <  1:832416/150‑1 (MQ=255)
                   gtcgtatgatACCAGCGATTTATTCGACATGGTCGGTGGTGCGCCGCTGGTGTTCATGTTGGTTGAAGGCACGCAGGGATTTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAAcgc                                                                                                                                 <  2:326382/145‑1 (MQ=255)
                         tgatACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCatat                                                                                                                           <  2:1327160/150‑1 (MQ=255)
                             aCCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGCCGCTTTCCGCGGTCTGCACGCGCATATTCTg                                                                                                                       >  1:1071320/1‑150 (MQ=255)
                              ccAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAACGCGTGATTGACGCTTTCCGCGGTCTGACCGCGCATATTGTgg                                                                                                                      >  1:51581/1‑150 (MQ=255)
                              ccAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTgg                                                                                                                      <  1:1423373/150‑1 (MQ=255)
                              ccAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTgg                                                                                                                      <  1:394811/150‑1 (MQ=255)
                               cAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTAGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGt                                                                                                                     >  1:236051/1‑150 (MQ=255)
                                aGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTg                                                                                                                    <  2:1301412/150‑1 (MQ=255)
                                 gCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGc                                                                                                                   <  1:1315087/150‑1 (MQ=255)
                                  cGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCa                                                                                                                  >  1:1280807/1‑150 (MQ=255)
                                   gATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAg                                                                                                                 >  2:561561/1‑150 (MQ=255)
                                    aTTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCACGGTGGTTGAAGGCACGCAGGGACTTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAgg                                                                                                                <  1:705019/150‑1 (MQ=255)
                                    aTTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAgg                                                                                                                <  1:577647/150‑1 (MQ=255)
                                       tactCGACTTGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGGGGTCTGAACGCGCATATTCTGGTGCAGGAat                                                                                                             <  1:592727/147‑1 (MQ=255)
                                       tAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCATGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAat                                                                                                             <  2:1631773/150‑1 (MQ=255)
                                        aaTCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAata                                                                                                            >  2:1688699/1‑150 (MQ=255)
                                        aaTCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAata                                                                                                            <  2:1200579/150‑1 (MQ=255)
                                            gACATGGTCGGTGGTGCGCCGCTGGTGGTCATGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATc                                                                                                        <  1:585076/150‑1 (MQ=255)
                                            gACAGGGGCGGTGGTGCGCCGCTGGTGGTCACGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGGGCTGGCGGAGACGCGGCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATc                                                                                                        <  1:88240/150‑1 (MQ=255)
                                             ccATGGTCGGTGGTGCGCCGCTGGTGGTCAAGGTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCa                                                                                                       <  2:1465289/149‑1 (MQ=255)
                                                  gTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAagag                                                                                                  <  1:1530843/150‑1 (MQ=255)
                                                   tCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAgg                                                                                                 <  2:161584/150‑1 (MQ=255)
                                                     ggtggtGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGgcg                                                                                               >  2:696692/1‑150 (MQ=255)
                                                      gtggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGgcgc                                                                                              >  1:843077/1‑150 (MQ=255)
                                                        ggtgCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCaa                                                                                            >  1:1704103/1‑150 (MQ=255)
                                                             gccgcTTGTGGTCTAGTTGGTGGATGGCTCGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCTGGCGGCGGATAGGGTGATTGACGCTTTCGGCGGTCTGTACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCTTGGGTg                                                                                       <  1:234737/150‑1 (MQ=255)
                                                             gccgcTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGTCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACTCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTg                                                                                       <  1:1688699/150‑1 (MQ=255)
                                                              ccgcTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGGCGTGCGGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGc                                                                                      <  2:1442581/150‑1 (MQ=255)
                                                                gcTGGAGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGGCAGGCGGCGGATAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCATGGGTGCGa                                                                                    <  2:1618480/150‑1 (MQ=255)
                                                                 cTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGat                                                                                   <  1:1263311/150‑1 (MQ=255)
                                                                 cTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGaa                                                                                   >  1:1679989/1‑149 (MQ=255)
                                                                       gtCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATTTATCAAAGAGTCGCAAGGGTGCGATATCCGc                                                                             >  2:1129789/1‑150 (MQ=255)
                                                                        tCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCt                                                                            >  2:1229605/1‑150 (MQ=255)
                                                                          aaGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGGCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGt                                                                          <  1:1288736/150‑1 (MQ=255)
                                                                            gttggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCt                                                                        >  2:120869/1‑150 (MQ=255)
                                                                             ttggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTg                                                                       <  1:195342/150‑1 (MQ=255)
                                                                               ggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGCCGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGgt                                                                     >  1:1225830/1‑150 (MQ=255)
                                                                               ggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGgt                                                                     <  2:1067350/150‑1 (MQ=255)
                                                                               ggttgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGgt                                                                     <  1:1530489/150‑1 (MQ=255)
                                                                                  tgAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGgttgt                                                                  <  2:1343619/150‑1 (MQ=255)
                                                                                    aaGGCCCGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCGGGTGCAGGAATATATCAAAGCGGCGCAAGGGTGCGCTATCCGCTGTCGGGttgttg                                                                >  1:501517/1‑150 (MQ=255)
                                                                                    aaGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGttgttg                                                                >  2:710589/1‑150 (MQ=255)
                                                                                      ggCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGc                                                              <  2:954166/150‑1 (MQ=255)
                                                                                        cACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTGTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGa                                                            >  2:175822/1‑150 (MQ=255)
                                                                                        cACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGa                                                            >  2:490595/1‑150 (MQ=255)
                                                                                         aCGCAGGGAATTGGCGTCGTGCGGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGAt                                                           <  2:843077/150‑1 (MQ=255)
                                                                                           gCAGGGAATTCGCGTCGCGCTGGCGGAGACGCGGCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGa                                                         <  2:231557/150‑1 (MQ=255)
                                                                                            cAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGaa                                                        <  2:999427/150‑1 (MQ=255)
                                                                                              gggAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGt                                                      <  1:710589/150‑1 (MQ=255)
                                                                                               ggAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTg                                                     <  1:561561/150‑1 (MQ=255)
                                                                                                   ttGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCg                                                 <  1:696692/150‑1 (MQ=255)
                                                                                                         tcgtGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGa                                           <  2:1280807/150‑1 (MQ=255)
                                                                                                           gtGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGTACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGAtt                                         <  1:534286/150‑1 (MQ=255)
                                                                                                            tGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGATGTGGTCGCTGc                                             <  1:233181/145‑1 (MQ=255)
                                                                                                            tGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGATGTGGTCGCTGc                                             >  2:233181/1‑145 (MQ=255)
                                                                                                            tGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTg                                        >  2:1149599/1‑150 (MQ=255)
                                                                                                            tGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTg                                        >  2:1143924/1‑150 (MQ=255)
                                                                                                                ggCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGACCGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAAcg                                    >  1:252678/1‑150 (MQ=255)
                                                                                                                ggCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAAcg                                    >  2:623548/1‑150 (MQ=255)
                                                                                                                      gaCGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACggcgggc                              <  1:1335836/150‑1 (MQ=255)
                                                                                                                         gcgTCAGGCGGCGGAAAGCGTGATTGACGCGTTCCGCGGGCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGaa                           <  1:129593/150‑1 (MQ=255)
                                                                                                                              cAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAagag                       <  1:120869/150‑1 (MQ=255)
                                                                                                                               aGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAgg                      >  1:88149/1‑150 (MQ=255)
                                                                                                                                ggcggcGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAggg                     <  1:1663438/150‑1 (MQ=255)
                                                                                                                                ggcggcGGAAAGCGGGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAggg                     <  1:522355/150‑1 (MQ=255)
                                                                                                                                ggcggaGGAAAGCGTGATTGACGCTTTCCGCTGTCTGAACGCGGATTTTCTGGTGCAGGAATATATCAATGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGGTGCGATTGAACGGCGGGCGAAAGAggg                     <  1:673042/150‑1 (MQ=255)
                                                                                                                                 gcggcgGAAAGGGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGGGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGc                    <  2:259469/150‑1 (MQ=255)
                                                                                                                                  ccgcggAAAGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCg                   >  2:1108043/3‑150 (MQ=255)
                                                                                                                                     cggAAAGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGAtt                >  1:1331192/1‑150 (MQ=255)
                                                                                                                                      ggAAAGCGTGATTGACGCTTTCCGCGGTCTGACCGCGCATATTCTGGTGCAGGAATATATAAAAGATTAACAAGGGTGAGATATACGGTGTCTGGTTGGTGGCGAGGAAGTGGTCGCTGAGATTCAACCGCGCGCGAAAGCggtagagtt               >  2:297051/1‑143 (MQ=255)
                                                                                                                                      ggAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGAttt               >  2:187242/1‑150 (MQ=255)
                                                                                                                                      ggAAAGCGTCAATCACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGAttt               >  2:681248/1‑150 (MQ=255)
                                                                                                                                       gAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGAtttt              >  1:145738/1‑150 (MQ=255)
                                                                                                                                        aaaGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTc             >  1:813800/1‑150 (MQ=255)
                                                                                                                                           gCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGtt          <  1:969966/150‑1 (MQ=255)
                                                                                                                                            cGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTc         >  1:47374/1‑150 (MQ=255)
                                                                                                                                            cGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTc         >  1:583581/1‑150 (MQ=255)
                                                                                                                                                aTTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAAt     >  1:737566/1‑150 (MQ=255)
                                                                                                                                                   gACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTg  >  2:1544054/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCACGGGCATTGCGCATTCGCCGGATGATACCAGCGATTTAATCGACATGGTCGGTGGTGCGCCGCTGGTGGTCAAGTTGGTTGAAGGCACGCAGGGAATTGGCGTCGTGCTGGCGGAGACGCGTCAGGCGGCGGAAAGCGTGATTGACGCTTTCCGCGGTCTGAACGCGCATATTCTGGTGCAGGAATATATCAAAGAGGCGCAAGGGTGCGATATCCGCTGTCTGGTTGTTGGCGATGAAGTGGTCGCTGCGATTGAACGGCGGGCGAAAGAGGGCGATTTTCGTTCCAATTTG  >  NC_000913/892313‑892608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: