Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 899,707 C→T 5.8% intergenic (+62/+137) rlmC → / ← artJ 23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913899,7070CT 5.8% 271.0 / 3.3 85intergenic (+62/+137)rlmC/artJ23S rRNA m(5)U747 methyltransferase, SAM‑dependent/arginine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (38/42);  new base T (2/3);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

TGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAA  >  NC_000913/899558‑899855
                                                                                                                                                     |                                                                                                                                                    
tGGGTTTCTTATTGAACTGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATTAAGTGATTACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATgcggc                                                                                                                                                      <  2:145995/150‑1 (MQ=39)
   gTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTa                                                                                                                                                   <  2:41683/150‑1 (MQ=255)
        gaaTTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACAATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGc                                                                                                                                              <  2:426369/148‑1 (MQ=255)
         tATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGTGTAAACGcc                                                                                                                                             <  1:1511269/150‑1 (MQ=255)
         tATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGcc                                                                                                                                             <  2:1354191/150‑1 (MQ=255)
         tATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGcc                                                                                                                                             >  2:847599/1‑150 (MQ=255)
               aCGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGTCTTATcc                                                                                                                                       <  1:877061/150‑1 (MQ=255)
               aCGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                       >  2:63532/1‑150 (MQ=255)
               aCGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                       <  2:318664/150‑1 (MQ=255)
               aCGGGTGCAGCTATTCGCTCTGGTCCCGCATACCGCTCACTATGATCTGCTGACTCTGGTGGTGACGCAATAAAAATGGCGCATGTGCGGCTTCAGATTGGAGACAAAGGGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                       <  1:281240/150‑1 (MQ=25)
                  ggTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGAGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                    >  2:835805/1‑150 (MQ=255)
                   gTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                   <  1:802000/150‑1 (MQ=255)
                   gTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                   <  2:612171/150‑1 (MQ=255)
                       aGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                                                                               <  1:1482678/150‑1 (MQ=255)
                        gCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa                                                                                                                              >  1:104867/1‑150 (MQ=255)
                           tttCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAg                                                                                                                           <  1:250609/150‑1 (MQ=255)
                              cGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGt                                                                                                                        >  2:1623837/1‑150 (MQ=255)
                               gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTg                                                                                                                       >  1:1505118/1‑150 (MQ=255)
                               gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTg                                                                                                                       <  2:279757/150‑1 (MQ=255)
                               gATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTg                                                                                                                       >  2:294046/1‑150 (MQ=255)
                                 tatGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCa                                                                                                                     >  1:568991/1‑150 (MQ=255)
                                  atGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCaa                                                                                                                    <  2:1016022/150‑1 (MQ=255)
                                     ttCCCGCATACCGCGCACTATGAAGCGCGGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAACCGCCTTATCCGGCCTACAAAAGCGTGCAAAtt                                                                                                                 <  2:1710374/150‑1 (MQ=255)
                                     ttCCCGCATACCGAGCCCTAAGAAGTGCTGCCTCTGCTGGAGAATCAATAAAAAATCCGCATGTGCGGCTTAAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTGATCCGTCCTACAAACTCGTGCAAAtt                                                                                                                 >  2:288827/1‑150 (MQ=21)
                                      tCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTc                                                                                                                <  2:721629/150‑1 (MQ=255)
                                      tCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTACAAATTc                                                                                                                <  2:89264/150‑1 (MQ=255)
                                       cccGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCa                                                                                                               >  2:843387/1‑150 (MQ=255)
                                         cGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAAt                                                                                                             <  1:727437/150‑1 (MQ=255)
                                           cATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATAc                                                                                                           <  1:1053277/150‑1 (MQ=255)
                                              aCCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACAtt                                                                                                        >  1:1268398/1‑150 (MQ=255)
                                                      cTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                                                                                >  2:1540232/1‑150 (MQ=255)
                                                      cTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                                                                                >  2:522498/1‑150 (MQ=255)
                                                      cTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                                                                                >  1:1474941/1‑150 (MQ=255)
                                                      cTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATCCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGc                                                                                                >  2:1604654/1‑150 (MQ=255)
                                                       tATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGcc                                                                                               >  1:1192573/1‑150 (MQ=255)
                                                           aaGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGt                                                                                           <  2:271996/150‑1 (MQ=255)
                                                             gTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAg                                                                                         >  2:212993/1‑150 (MQ=255)
                                                                 tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGTGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCt                                                                                     >  2:141164/1‑150 (MQ=255)
                                                                 tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGTGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCt                                                                                     >  2:141165/1‑150 (MQ=255)
                                                                 tGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCt                                                                                     <  2:1153893/150‑1 (MQ=255)
                                                                  gACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTg                                                                                    <  1:1521507/150‑1 (MQ=255)
                                                                   aCGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGa                                                                                   <  1:909242/150‑1 (MQ=255)
                                                                     gctgctGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATa                                                                                 <  2:1407601/150‑1 (MQ=255)
                                                                          tgGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGt                                                                            <  2:104867/150‑1 (MQ=255)
                                                                           ggTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTa                                                                           >  2:626769/1‑150 (MQ=255)
                                                                             tGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAgc                                                                         >  1:1310073/1‑150 (MQ=255)
                                                                             ggAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAgc                                                                         <  2:1041103/149‑1 (MQ=255)
                                                                                  cAATAAAAAAGCCGCATGTGCGGCTTCATTATGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATc                                                                    <  1:1412013/150‑1 (MQ=255)
                                                                                   caatctgaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCa                                                                   >  2:984140/8‑150 (MQ=255)
                                                                                   aaTAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCa                                                                   >  1:1567879/1‑150 (MQ=255)
                                                                                    aatctgaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAg                                                                  >  1:1009416/7‑150 (MQ=255)
                                                                                     atctgaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                 >  2:741916/6‑150 (MQ=255)
                                                                                     atctgaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                 >  2:811880/6‑150 (MQ=255)
                                                                                     atctgaaGCCGCACATGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAgg                                                                 >  2:844035/6‑150 (MQ=255)
                                                                                       aaaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACAAAAGCGTGAAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                               >  1:1031555/1‑150 (MQ=255)
                                                                                        aaaaGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                              <  2:1268398/150‑1 (MQ=255)
                                                                                             ccGCATGTTCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCAGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCATATTCAATACATTGCATTGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTa                                                         <  2:1191859/150‑1 (MQ=255)
                                                                                               gCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTAcc                                                       >  1:1130923/1‑150 (MQ=255)
                                                                                                cATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCt                                                      <  2:1310073/150‑1 (MQ=255)
                                                                                                  tgtgCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCttt                                                    >  2:980196/1‑150 (MQ=255)
                                                                                                   atccGGCTTAAGATTGCTTACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTg                                                   <  1:527969/147‑1 (MQ=255)
                                                                                                    tgCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAAAGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGt                                                  <  2:771819/150‑1 (MQ=255)
                                                                                                     gCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                >  2:930990/1‑135 (MQ=255)
                                                                                                     gCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                <  1:930990/135‑1 (MQ=255)
                                                                                                     gCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGtc                                                 >  2:1244392/1‑150 (MQ=255)
                                                                                                     gCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGtc                                                 <  1:843387/150‑1 (MQ=255)
                                                                                                        gCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGtcatc                                              <  1:294046/150‑1 (MQ=255)
                                                                                                         cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGtcatca                                             <  2:568991/150‑1 (MQ=255)
                                                                                                         cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATACAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTTGCGCAGCAGGCAATTTTACCTTTGtcatca                                             >  2:330178/1‑150 (MQ=255)
                                                                                                          ttCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAg                                            <  2:1474941/150‑1 (MQ=255)
                                                                                                              gATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGTGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGtctc                                        <  1:141164/150‑1 (MQ=255)
                                                                                                              gATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGTGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGtcta                                        <  1:141165/150‑2 (MQ=255)
                                                                                                               aTTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCa                                       <  1:899753/150‑1 (MQ=255)
                                                                                                                  gCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGc                                    >  2:1400120/1‑150 (MQ=255)
                                                                                                                        aaaGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTGGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCcgcaac                              >  1:679814/1‑147 (MQ=255)
                                                                                                                        aaaGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGt                              <  2:1315979/150‑1 (MQ=255)
                                                                                                                           gTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGc                           <  1:1356232/150‑1 (MQ=255)
                                                                                                                                  ttgttTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCTAGCCGCGGTTGCGGCTTTc                    <  1:741916/150‑1 (MQ=255)
                                                                                                                                  ttgttTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTc                    <  1:811880/150‑1 (MQ=255)
                                                                                                                                    gttTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTg                  >  2:1441069/1‑150 (MQ=255)
                                                                                                                                        aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATc              >  2:100705/3‑150 (MQ=25)
                                                                                                                                           ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTa           >  2:1648689/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTa           >  2:1022944/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTa           <  2:1009416/150‑1 (MQ=255)
                                                                                                                                              gATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACtg        <  1:847599/150‑1 (MQ=255)
                                                                                                                                                tGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACtgtg      >  1:214382/1‑150 (MQ=255)
                                                                                                                                                    gcgTAAACGCCTTATCCGGCCTACAAAAGCGTGCTAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGATTTCTGAATCTTACTGTGGGaa  >  1:230103/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGGTTTCGTATTGAACGGGTGCAGCTTTTCGATATGTTCCCGCATACCGCGCACTATGAAGTGCTGACGCTGCTGGTGAAGCAATAAAAAAGCCGCATGTGCGGCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAATACATTGCATGGGCCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTACCTTTGTCATCAGTCTCAAGCCGCGGTTGCGGCTTTCTGAATCTTACTGTGGGAA  >  NC_000913/899558‑899855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: