Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 900,710 G→C 5.2% intergenic (‑135/+156) artJ ← / ← artM arginine ABC transporter periplasmic binding protein/arginine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913900,7100GC 5.2% 447.5 / 9.5 130intergenic (‑135/+156)artJ/artMarginine ABC transporter periplasmic binding protein/arginine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (68/55);  new base C (5/2);  total (73/57)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 130 total aligned reads displayed.

ACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGAATGTACG  >  NC_000913/900562‑900857
                                                                                                                                                     |                                                                                                                                                   
actaactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGt                                                                                                                                                    >  2:1108087/1‑150 (MQ=255)
 ctaactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTTAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTg                                                                                                                                                   >  1:359136/1‑150 (MQ=255)
  taactTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTgg                                                                                                                                                  <  1:34351/150‑1 (MQ=255)
       ttttCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGt                                                                                                                                             <  2:1007929/150‑1 (MQ=255)
       ttttCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGt                                                                                                                                             >  1:760240/1‑150 (MQ=255)
         ttCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTcc                                                                                                                                           <  2:1435318/150‑1 (MQ=255)
         ttCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTcc                                                                                                                                           <  2:606291/150‑1 (MQ=255)
         ttCATGATGGAACACCCGGCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTcc                                                                                                                                           <  1:85037/150‑1 (MQ=255)
          tCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCg                                                                                                                                          <  2:802094/150‑1 (MQ=255)
           cATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGt                                                                                                                                         <  1:1495981/150‑1 (MQ=255)
           cATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGt                                                                                                                                         >  2:1179903/1‑150 (MQ=255)
           cATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTAAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGt                                                                                                                                         >  1:1682998/1‑150 (MQ=255)
            atgatgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTc                                                                                                                                        <  1:1545901/150‑1 (MQ=255)
             tgatgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCt                                                                                                                                       >  2:1587539/1‑150 (MQ=255)
               atgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACCCACTAAGTTGTCCGTCttt                                                                                                                                     <  1:1659474/150‑2 (MQ=255)
                tgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCa                                                                                                                                    >  2:500123/1‑150 (MQ=255)
                tgGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCa                                                                                                                                    <  1:1250706/150‑1 (MQ=255)
                    aCTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATtat                                                                                                                                <  1:661938/150‑1 (MQ=255)
                    aCTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACCCACTAAGTTGTCCGTCTTTTTtat                                                                                                                                <  1:1432585/150‑1 (MQ=39)
                     cTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGTATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATtata                                                                                                                               <  1:1135860/150‑1 (MQ=255)
                          gTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGGTGTCCGTCTTCATTATAAGAAt                                                                                                                          <  2:1499686/150‑1 (MQ=255)
                             tGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTa                                                                                                                       <  2:1713458/150‑1 (MQ=255)
                                cAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGc                                                                                                                    >  2:1052467/1‑150 (MQ=255)
                                cAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGc                                                                                                                    >  1:1627827/1‑150 (MQ=255)
                                    cTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTa                                                                                                                >  1:1698706/1‑150 (MQ=255)
                                     ttATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTAt                                                                                                               >  1:1508773/1‑150 (MQ=255)
                                      tATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGTTTGTCCGTCTTCATTATAAGAATTTATGCACTATg                                                                                                              >  1:1512755/1‑150 (MQ=255)
                                      tATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATg                                                                                                              >  1:1519251/1‑150 (MQ=255)
                                      tATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATg                                                                                                              >  1:1331346/1‑150 (MQ=255)
                                        tGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTa                                                                                                            >  2:319577/1‑150 (MQ=255)
                                         gATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAg                                                                                                           >  1:767835/1‑150 (MQ=255)
                                          aTTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAgg                                                                                                          <  1:478910/150‑1 (MQ=255)
                                             tttGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCg                                                                                                       <  1:1479922/150‑1 (MQ=255)
                                               tGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGa                                                                                                     >  2:227723/1‑150 (MQ=255)
                                                 gCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTTTAAGAATTTATGCACTATGTAGGCCGGATa                                                                                                   >  1:1081928/1‑150 (MQ=255)
                                                 gCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATa                                                                                                   >  1:1207579/1‑150 (MQ=255)
                                                         tGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCTTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTcc                                                                                           >  1:250397/1‑150 (MQ=255)
                                                         tGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCATCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTcc                                                                                           >  2:1268989/1‑150 (MQ=255)
                                                             gCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCAGTCTTCATTATAAGAATATATTCACTATGTAGGCCGGATAAGGCGTCCccgc                                                                                       >  2:1509022/1‑150 (MQ=255)
                                                              cATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCccgcc                                                                                      <  1:1312878/150‑1 (MQ=255)
                                                                  ggATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTTAATAATTTAAATGAAATAAAAACGACGGACAACTTTGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCAt                                                                                  >  1:275234/1‑150 (MQ=255)
                                                                  ggATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCAt                                                                                  >  1:864887/1‑150 (MQ=255)
                                                                  ggATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCAt                                                                                  >  2:42393/1‑150 (MQ=255)
                                                                   gATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATc                                                                                 >  2:1575996/1‑150 (MQ=255)
                                                                    aTAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATcc                                                                                <  1:1011801/150‑1 (MQ=255)
                                                                       aCAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGc                                                                             >  1:525442/1‑150 (MQ=255)
                                                                       aCAATGCCATCAAGTGAATTTATATGCAATAAACATGAATAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGc                                                                             <  1:1521356/150‑1 (MQ=255)
                                                                       aCAATGCCATCAAGTGAATTTATAAGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGc                                                                             >  2:1185021/1‑150 (MQ=255)
                                                                           tGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAg                                                                         <  2:1123369/150‑1 (MQ=255)
                                                                           tGCCATCAAGTGAATTTATATGCAATAAAAATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAg                                                                         <  1:649182/150‑1 (MQ=255)
                                                                            gCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAgg                                                                        <  1:1455661/150‑1 (MQ=255)
                                                                             ccATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGc                                                                       >  1:1256981/1‑150 (MQ=255)
                                                                                tCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCAcc                                                                    >  2:656134/1‑150 (MQ=255)
                                                                                    gTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGc                                                                <  1:1025603/150‑1 (MQ=255)
                                                                                      gAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTACGTCTTCATTATAAGAATTTATGCACTATGAAGGCCGGATAAGGAGTCCCCGCCGCATCCGGCACAGGCACCTTGCTg                                                              >  1:122653/1‑150 (MQ=255)
                                                                                         tttATATGCAATAAACATGATTAAATAATTTAAATGAATTAAAAAAGACGGACAACTTAGTGGGTTGTCAGTCTTCATTATAATAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATg                                                           <  1:1509022/150‑1 (MQ=255)
                                                                                           tataTGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTc                                                         >  1:815122/1‑150 (MQ=255)
                                                                                             tatGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTg                                                       <  2:1373965/150‑1 (MQ=255)
                                                                                               tGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGAt                                                     >  1:883957/1‑150 (MQ=255)
                                                                                                 cAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGAttc                                                   <  2:219750/150‑3 (MQ=255)
                                                                                                 cAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGc                                                   <  1:142612/150‑1 (MQ=255)
                                                                                                 cAAAAAACATGATTAAATAATTTAAATGAAATAAAAAAAGACGTACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATg                                                    <  2:249593/150‑1 (MQ=255)
                                                                                                  aaTAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCg                                                  >  1:806887/1‑150 (MQ=255)
                                                                                                    tAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGAc                                                <  2:767835/150‑1 (MQ=255)
                                                                                                    tAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGAc                                                <  2:142329/150‑1 (MQ=255)
                                                                                                     aaaCATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACg                                               <  2:1508773/150‑1 (MQ=255)
                                                                                                      aaCATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGc                                              >  1:1205719/1‑150 (MQ=255)
                                                                                                      aaCATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGc                                              >  2:660369/1‑150 (MQ=255)
                                                                                                        cATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTTTGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTg                                            >  2:1714444/1‑150 (MQ=255)
                                                                                                         aTGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTgg                                           >  1:1418840/1‑150 (MQ=255)
                                                                                                           gATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGgcg                                         >  1:1711553/1‑150 (MQ=255)
                                                                                                                  taatTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCACCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTa                                  >  1:1484399/1‑150 (MQ=255)
                                                                                                                   aatTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTAt                                 <  2:1207579/150‑1 (MQ=255)
                                                                                                                    atTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATc                                <  1:891703/150‑1 (MQ=255)
                                                                                                                    atTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATc                                <  2:795581/150‑1 (MQ=255)
                                                                                                                      ttAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAg                              <  2:250397/150‑1 (MQ=255)
                                                                                                                       tAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGa                             >  2:426729/1‑150 (MQ=255)
                                                                                                                        aaaTGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGAc                            >  1:382713/1‑150 (MQ=255)
                                                                                                                        aaaTGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGAc                            <  2:864887/150‑1 (MQ=255)
                                                                                                                         aaTGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGAcc                           <  1:439155/150‑1 (MQ=255)
                                                                                                                             aaaTAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACa                       >  2:564387/1‑150 (MQ=255)
                                                                                                                             aaaTAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACa                       >  2:557642/1‑150 (MQ=255)
                                                                                                                                tAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACaaaa                    >  1:1159405/1‑150 (MQ=255)
                                                                                                                                tAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACaaaa                    >  1:1165373/1‑150 (MQ=255)
                                                                                                                                 taatgaaGACGGACAACCCACTAAGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACAGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACaaaa                    >  2:1432893/6‑150 (MQ=38)
                                                                                                                                     aaaGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAAcccc                >  1:825920/1‑150 (MQ=255)
                                                                                                                                       aGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAAcccccc              >  1:816994/1‑150 (MQ=255)
                                                                                                                                       aGACGGACAACCCACTAAGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAAcccccc              >  1:1329556/1‑150 (MQ=38)
                                                                                                                                        gACGGACAACCCACTAAGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACCGGCCCCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCg             >  2:1038230/1‑150 (MQ=38)
                                                                                                                                        gACGGACAACCCACTAAGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCg             >  1:345491/1‑150 (MQ=38)
                                                                                                                                         aCGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCgg            <  2:1682998/150‑1 (MQ=255)
                                                                                                                                         aCGGACAACCCACTAAGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCgg            >  1:1049653/1‑150 (MQ=38)
                                                                                                                                           ggACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCg          >  1:1254727/1‑150 (MQ=255)
                                                                                                                                           ggACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCg          >  2:879712/1‑150 (MQ=255)
                                                                                                                                            gACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGa         >  2:682136/1‑150 (MQ=255)
                                                                                                                                              cAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGAAt       >  1:49557/1‑150 (MQ=255)
                                                                                                                                               aaCTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGAATg      >  2:835016/1‑150 (MQ=255)
                                                                                                                                                 cTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGAATGTa    >  2:1518849/1‑150 (MQ=255)
                                                                                                                                                   tAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGCCATACAAAACCCCCCGGCGAATGTACg  >  1:1250956/1‑150 (MQ=255)
                                                                                                                                                   tAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGAATGTACg  <  2:1562219/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACTAACTTTTTCATGATGGAACTCCCGTCTGTCAATCTTATGATTTTTGGCCGTGTCTGCGGCATGGGATAACAATGCCATCAAGTGAATTTATATGCAATAAACATGATTAAATAATTTAAATGAAATAAAAAAGACGGACAACTTAGTGGGTTGTCCGTCTTCATTATAAGAATTTATGCACTATGTAGGCCGGATAAGGCGTCCCCGCCGCATCCGGCACAGGCACCGTGCTGATGTCTGATGCGACGCTGGCGCGTCTTATCAGACCTACAAAACCCCCCGGCGAATGTACG  >  NC_000913/900562‑900857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: