Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 925,574 T→C 10.3% intergenic (+34/+310) clpA → / ← serW ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913925,5740TC10.3% 303.0 / 22.9 116intergenic (+34/+310)clpA/serWATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (44/60);  new base C (7/5);  total (51/65)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01

Only 100 of 118 total aligned reads displayed.

TGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTG  >  NC_000913/925425‑925717
                                                                                                                                                     |                                                                                                                                               
tgTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAAt                                                                                                                                                 >  2:1638528/1‑150 (MQ=255)
    gggTGCGCTGGTGGCCGGCGGTCAGGTCACCGTCGCGCGGGATAAAGAGAAAACTGAGCTGACTTACGGATTCCAGAGTGCACAAACGCACAAGGCGGAAGCAGCGCATTAATCTGAGTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCg                                                                                                                                             <  1:1072765/149‑1 (MQ=255)
      gTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGaa                                                                                                                                           >  2:432950/1‑150 (MQ=255)
      gTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGaa                                                                                                                                           <  1:1235285/150‑1 (MQ=255)
          gCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAagag                                                                                                                                       <  2:374515/150‑1 (MQ=255)
           cTGGTGGACGGCGGTCAGGTCACCGTCGCGCAGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTATTCTCCAAAGAgg                                                                                                                                      >  2:129504/1‑150 (MQ=255)
            tggtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGt                                                                                                                                     <  2:255906/150‑1 (MQ=255)
              gtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTa                                                                                                                                   <  2:1009766/150‑1 (MQ=255)
               tggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTACACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                  <  1:332081/150‑1 (MQ=255)
               tggACGGCGGTCAGGTCACCGGCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                  <  1:719517/150‑1 (MQ=255)
               gggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                  <  2:1512778/149‑1 (MQ=255)
                 gACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACgg                                                                                                                                >  1:1279752/1‑150 (MQ=255)
                  aCGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGa                                                                                                                               <  1:1468181/150‑1 (MQ=255)
                  aCGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACATACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGa                                                                                                                               <  2:96777/150‑1 (MQ=255)
                      cggTCAGGACACCGTCGCGCTGGATAAAGACCAAACTGAGCTGACTTACGGATGCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGACttg                                                                                                                           <  1:98995/150‑2 (MQ=255)
                       ggtcaggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACtttt                                                                                                                          <  2:63411/150‑1 (MQ=255)
                          caggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                       <  1:1183120/150‑1 (MQ=255)
                            ggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGAc                                                                                                                              <  2:1506927/141‑1 (MQ=255)
                            ggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGAc                                                                                                                              >  1:1506927/1‑141 (MQ=255)
                             gtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTa                                                                                                                    >  1:1465670/1‑150 (MQ=255)
                               caCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                       >  2:1241074/1‑145 (MQ=255)
                               caCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                       <  1:1241074/145‑1 (MQ=255)
                                aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                 <  1:784324/150‑1 (MQ=255)
                                aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                 <  1:935002/150‑1 (MQ=255)
                                aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                 <  1:16647/150‑1 (MQ=255)
                                        gcTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTggagg                                                                                                         >  2:132400/1‑150 (MQ=255)
                                          tgtaTAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTTATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGgaggag                                                                                                       <  2:141480/147‑1 (MQ=255)
                                          tGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGgaggag                                                                                                       >  1:120261/1‑150 (MQ=255)
                                           ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGaggagg                                                                                                      >  2:495678/1‑150 (MQ=255)
                                           ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGaggagg                                                                                                      >  2:286174/1‑150 (MQ=255)
                                           ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGGGGaggagg                                                                                                      <  1:631195/150‑1 (MQ=255)
                                           ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGCCAAGTCCGTAATCTCGAATGAGGTTACGGACTTTTTGTTTATGGGGGGGaggagg                                                                                                      <  2:516790/150‑1 (MQ=255)
                                              aaaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTc                                                                                                   <  1:1340177/149‑1 (MQ=255)
                                               aaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCa                                                                                                  <  2:1172108/150‑1 (MQ=255)
                                                aaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAg                                                                                                 >  1:1541728/1‑150 (MQ=255)
                                                  tagaAAAATGCGCTGACTTACGGCTTCCAGAGTGCACAAACGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                               <  2:805740/149‑1 (MQ=255)
                                                  tagaAAAATGAGCTGACTTACGGATTCCAGAGTGAACAAAAGCACAGGGCTGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGGTCAGAc                                                                                               <  2:1312603/149‑1 (MQ=255)
                                                  gagaAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                               <  1:1638528/150‑1 (MQ=255)
                                                  gagaAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                               <  2:609716/150‑1 (MQ=255)
                                                      aaaaTGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGAGAATTAATCTGATTGTTAGGTAGGTTGGTCAATTCGTTAATCTAGAAAGATGTTACGGACTTTTTGTTTATGTGGTGGAGGAGGTTCAGAccata                                                                                           >  2:33432/1‑147 (MQ=255)
                                                          tGAGCTGACTTACGGATTGCAGACTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCATGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTTGAGGAGGTTCAGACCCtttttt                                                                                       <  2:1044757/150‑1 (MQ=255)
                                                           gAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGCCCCttttttt                                                                                      >  2:1358676/1‑150 (MQ=255)
                                                           gAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCttttttt                                                                                      <  2:120261/150‑1 (MQ=255)
                                                             gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                    >  1:833392/1‑150 (MQ=255)
                                                             gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                    <  1:169897/150‑1 (MQ=255)
                                                              cTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAat                                                                                   <  2:1331859/150‑1 (MQ=255)
                                                                gACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTATGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatga                                                                                 <  1:763379/150‑1 (MQ=255)
                                                                 aCTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgat                                                                                <  2:1488576/150‑1 (MQ=255)
                                                                   ttACGGATTCCAGAGTACACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatga                                                                              <  1:873637/150‑1 (MQ=255)
                                                                   ttACGGATTCCAGAGGGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatga                                                                              <  1:128066/150‑1 (MQ=255)
                                                                            ccAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAgtt                                                                     >  1:1146042/1‑150 (MQ=255)
                                                                               gagTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAgttgtt                                                                  <  2:1248205/150‑1 (MQ=255)
                                                                                agagCAGAACAGCACAAGGCTGAAGCAGAGCATTAATCTGATTGTTAGGTTGGTTGGTCAAGTCCGTTATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAGCCTTTTTTTAATGATGATGGTAAGttgttg                                                                 <  1:260496/147‑1 (MQ=255)
                                                                                    cacaAAAGCACAAGGCGGCAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAACCTTTTTTTAATGATGATGGTAAGTTGTTGATaa                                                             >  1:241353/1‑150 (MQ=255)
                                                                                    cacaAAAGCACAAGGCGGAAGCAGCGCATTTATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATaa                                                             >  2:386890/1‑150 (MQ=255)
                                                                                    cacaAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGAtta                                                             >  2:801651/1‑148 (MQ=255)
                                                                                     acaAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACTGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTTTTGATAAt                                                            >  1:150037/1‑150 (MQ=255)
                                                                                        aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                         <  1:1030672/150‑1 (MQ=255)
                                                                                        aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                         <  2:358600/150‑1 (MQ=255)
                                                                                        aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTGTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                         >  1:457076/1‑150 (MQ=255)
                                                                                        aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTACGTAATCTCGAAAGAGGTTACTGACTTTTTGTTTATGGTGTTGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                         <  1:238917/150‑1 (MQ=255)
                                                                                          aGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGtg                                                       >  1:864404/1‑150 (MQ=255)
                                                                                           gCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTAACGGACTATTTGTTTATGGGGTGGAGGAGGTTCAAACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGtgc                                                      >  2:1534367/1‑150 (MQ=255)
                                                                                                aGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCgg                                                 <  2:1279752/150‑1 (MQ=255)
                                                                                                    ggAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAg                                             >  2:575910/1‑150 (MQ=255)
                                                                                                         catcgcATTAATGTTTTTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTTTTTATGGGGTGGAGGTGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAg                                        <  2:1465670/147‑1 (MQ=255)
                                                                                                           gcgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGa                                      >  1:908870/1‑150 (MQ=255)
                                                                                                            cgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGAt                                     >  1:1101619/1‑150 (MQ=255)
                                                                                                            cgcATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGAt                                     <  1:778997/150‑1 (MQ=255)
                                                                                                             gcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATa                                    <  1:132400/150‑1 (MQ=255)
                                                                                                             gcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTACGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATa                                    >  2:602386/1‑150 (MQ=255)
                                                                                                               aTTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaa                                  <  1:1639406/150‑1 (MQ=255)
                                                                                                                ttAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaa                                 <  1:575910/150‑1 (MQ=255)
                                                                                                                  aaTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaaaa                               >  1:856418/1‑150 (MQ=255)
                                                                                                                  aaTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaaaa                               >  2:916027/1‑150 (MQ=255)
                                                                                                                   aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                              <  1:495678/150‑1 (MQ=255)
                                                                                                                   aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                              <  2:856418/150‑1 (MQ=255)
                                                                                                                   aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                              <  1:286174/150‑1 (MQ=255)
                                                                                                                   aTCTGATTGTTACGTAGGTTGGTGAAGTCCGTAATGTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATTATTGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                              <  1:154779/150‑1 (MQ=255)
                                                                                                                    tCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAGGTAAGGATAAAAAAggg                            >  2:1250508/1‑150 (MQ=255)
                                                                                                                      tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                           >  1:978439/1‑150 (MQ=255)
                                                                                                                       gATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtg                          >  1:1700807/1‑150 (MQ=255)
                                                                                                                         ttGTTAGTTAGGTTTGTCAAGTCCGTAATCTCGATAGAGGTTACGTACTTTTTGTATATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtgct                        <  1:658844/150‑1 (MQ=255)
                                                                                                                           gTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtgctgc                      <  2:1087083/150‑1 (MQ=255)
                                                                                                                              aggtaggtTGGTCAAGTCCATAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAgg                   <  1:58775/150‑1 (MQ=255)
                                                                                                                              aggtagggTGGTCAAGTCCGTAATCTCGAAATAGGTTACGGACTTTTTGTTTATGGGGGGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTGGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAgg                   <  1:1312402/150‑1 (MQ=255)
                                                                                                                                gtaggtTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTTGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGgat                 >  1:160477/1‑149 (MQ=255)
                                                                                                                                  aggtTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGaa               <  2:1541728/150‑1 (MQ=255)
                                                                                                                                     aacaaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg            >  2:1569607/7‑150 (MQ=38)
                                                                                                                                      tGGTCAAGTCCGTAATCTCGATAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATggg           >  1:273603/1‑150 (MQ=255)
                                                                                                                                      acaaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATggg           >  1:628994/6‑150 (MQ=38)
                                                                                                                                       ggTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTCCAGGAGAATGGGa          >  2:297347/1‑150 (MQ=255)
                                                                                                                                       ggTAAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTCTGGGGGGGAGGCGGCTCAGACCCTTTTTTTAATGTTGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGa          <  1:1534367/150‑1 (MQ=255)
                                                                                                                                        aaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGTGAAGGTAAGGATAAAAAAGGGTTCTGCAGGAGAATGGGAt         >  2:253000/4‑150 (MQ=255)
                                                                                                                                          aaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg            <  2:231913/144‑1 (MQ=255)
                                                                                                                                           aaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTATGTTGTTGATTATTAGTGCTGAGGGATGGTAAGGATAAAAAAGGGTGCAGCAGGAGAATGGGATGGt      >  2:269208/1‑150 (MQ=255)
                                                                                                                                             gTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTCTGGGGTGGAGGAGGTTCAGACCCTTTTTTTTATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt    <  1:1254280/150‑1 (MQ=255)
                                                                                                                                             gTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt    >  2:1582284/1‑150 (MQ=255)
                                                                                                                                             gTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt    >  2:1227959/1‑150 (MQ=255)
                                                                                                                                               ccGTAATCTCGAAAGAGGGTACGGACTTTTTGTTTATGGGGTGGAGGAGGTGCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTg  <  1:1358676/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
TGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTG  >  NC_000913/925425‑925717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: