Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 925,578 G→T 10.3% intergenic (+38/+306) clpA → / ← serW ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913925,5780GT10.3% 323.7 / 22.7 117intergenic (+38/+306)clpA/serWATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (44/60);  new base T (7/5);  total (52/65)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.52e-01

Only 100 of 119 total aligned reads displayed.

TGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAAC  >  NC_000913/925429‑925727
                                                                                                                                                     |                                                                                                                                                     
gggTGCGCTGGTGGCCGGCGGTCAGGTCACCGTCGCGCGGGATAAAGAGAAAACTGAGCTGACTTACGGATTCCAGAGTGCACAAACGCACAAGGCGGAAGCAGCGCATTAATCTGAGTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCg                                                                                                                                                       <  1:1072765/149‑1 (MQ=255)
  gTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGaa                                                                                                                                                     >  2:432950/1‑150 (MQ=255)
  gTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGaa                                                                                                                                                     <  1:1235285/150‑1 (MQ=255)
      gCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAagag                                                                                                                                                 <  2:374515/150‑1 (MQ=255)
        tggtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGt                                                                                                                                               <  2:255906/150‑1 (MQ=255)
          gtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTa                                                                                                                                             <  2:1009766/150‑1 (MQ=255)
           tggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTACACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                            <  1:332081/150‑1 (MQ=255)
           tggACGGCGGTCAGGTCACCGGCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                            <  1:719517/150‑1 (MQ=255)
           gggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                            <  2:1512778/149‑1 (MQ=255)
            ggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACg                                                                                                                                           <  1:1322825/150‑1 (MQ=255)
             gACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACgg                                                                                                                                          >  1:1279752/1‑150 (MQ=255)
             gACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACgg                                                                                                                                          <  2:332224/150‑1 (MQ=255)
              aCGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGa                                                                                                                                         <  1:1468181/150‑1 (MQ=255)
              aCGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACATACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGa                                                                                                                                         <  2:96777/150‑1 (MQ=255)
                   ggtcaggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACtttt                                                                                                                                    <  2:63411/150‑1 (MQ=255)
                      caggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                 <  1:1183120/150‑1 (MQ=255)
                        ggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGAc                                                                                                                                        <  2:1506927/141‑1 (MQ=255)
                        ggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGAc                                                                                                                                        >  1:1506927/1‑141 (MQ=255)
                           caCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                 >  2:1241074/1‑145 (MQ=255)
                           caCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                 <  1:1241074/145‑1 (MQ=255)
                            aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                           <  1:935002/150‑1 (MQ=255)
                            aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                           <  1:16647/150‑1 (MQ=255)
                            aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                           <  1:784324/150‑1 (MQ=255)
                              cGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgggg                                                                                                                         >  1:12272/1‑150 (MQ=255)
                                   cgcTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTggag                                                                                                                    >  1:629740/1‑150 (MQ=255)
                                    gcTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTggagg                                                                                                                   >  2:132400/1‑150 (MQ=255)
                                       ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGaggagg                                                                                                                >  2:286174/1‑150 (MQ=255)
                                       ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGGGGaggagg                                                                                                                <  1:631195/150‑1 (MQ=255)
                                       ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGCCAAGTCCGTAATCTCGAATGAGGTTACGGACTTTTTGTTTATGGGGGGGaggagg                                                                                                                <  2:516790/150‑1 (MQ=255)
                                          aaaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTc                                                                                                             <  1:1340177/149‑1 (MQ=255)
                                           aaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCa                                                                                                            <  2:1172108/150‑1 (MQ=255)
                                            aaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAg                                                                                                           >  1:1541728/1‑150 (MQ=255)
                                             agagAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGa                                                                                                          >  2:435591/1‑150 (MQ=255)
                                              tagaAAAATGCGCTGACTTACGGCTTCCAGAGTGCACAAACGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                                         <  2:805740/149‑1 (MQ=255)
                                              tagaAAAATGAGCTGACTTACGGATTCCAGAGTGAACAAAAGCACAGGGCTGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGGTCAGAc                                                                                                         <  2:1312603/149‑1 (MQ=255)
                                              gagaAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                                         <  2:609716/150‑1 (MQ=255)
                                              gagaAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                                         <  1:1638528/150‑1 (MQ=255)
                                                  aaaaTGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGAGAATTAATCTGATTGTTAGGTAGGTTGGTCAATTCGTTAATCTAGAAAGATGTTACGGACTTTTTGTTTATGTGGTGGAGGAGGTTCAGAccata                                                                                                     >  2:33432/1‑147 (MQ=255)
                                                      tGAGCTGACTTACGGATTGCAGACTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCATGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTTGAGGAGGTTCAGACCCtttttt                                                                                                 <  2:1044757/150‑1 (MQ=255)
                                                       gAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGCCCCttttttt                                                                                                >  2:1358676/1‑150 (MQ=255)
                                                         gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                              >  1:384064/1‑150 (MQ=255)
                                                         gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                              <  1:169897/150‑1 (MQ=255)
                                                         gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                              >  1:833392/1‑150 (MQ=255)
                                                          cTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAat                                                                                             <  2:1331859/150‑1 (MQ=255)
                                                            gACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTATGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatga                                                                                           <  1:763379/150‑1 (MQ=255)
                                                             aCTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgat                                                                                          <  2:1488576/150‑1 (MQ=255)
                                                              cTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatg                                                                                         >  2:1639406/1‑150 (MQ=255)
                                                               ttACGGATTCCAGAGTACACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatga                                                                                        <  1:873637/150‑1 (MQ=255)
                                                                        ccAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATTATGGTAAgtt                                                                               >  2:1254280/1‑150 (MQ=255)
                                                                        ccAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAgtt                                                                               >  1:1146042/1‑150 (MQ=255)
                                                                          agagTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTATTTTAATGATGATGGTAAgttct                                                                             >  1:80490/1‑148 (MQ=255)
                                                                           gagTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAgttgtt                                                                            <  2:1248205/150‑1 (MQ=255)
                                                                            agagCAGAACAGCACAAGGCTGAAGCAGAGCATTAATCTGATTGTTAGGTTGGTTGGTCAAGTCCGTTATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAGCCTTTTTTTAATGATGATGGTAAGttgttg                                                                           <  1:260496/147‑1 (MQ=255)
                                                                                cacaAAAGCACAAGGCGGCAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAACCTTTTTTTAATGATGATGGTAAGTTGTTGATaa                                                                       >  1:241353/1‑150 (MQ=255)
                                                                                cacaAAAGCACAAGGCGGAAGCAGCGCATTTATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATaa                                                                       >  2:386890/1‑150 (MQ=255)
                                                                                cacaAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGAtta                                                                       >  2:801651/1‑148 (MQ=255)
                                                                                 acaAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACTGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTTTTGATAAt                                                                      >  1:150037/1‑150 (MQ=255)
                                                                                    aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                   <  2:1525360/150‑1 (MQ=255)
                                                                                    aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                   <  1:1030672/150‑1 (MQ=255)
                                                                                    aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTGTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                   >  1:457076/1‑150 (MQ=255)
                                                                                    aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTACGTAATCTCGAAAGAGGTTACTGACTTTTTGTTTATGGTGTTGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                   <  1:238917/150‑1 (MQ=255)
                                                                                            aGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCgg                                                           <  2:1279752/150‑1 (MQ=255)
                                                                                                ggAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAg                                                       >  2:575910/1‑150 (MQ=255)
                                                                                                     catcgcATTAATGTTTTTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTTTTTATGGGGTGGAGGTGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAg                                                  <  2:1465670/147‑1 (MQ=255)
                                                                                                       gcgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGTGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGa                                                >  1:914959/1‑150 (MQ=255)
                                                                                                       gcgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGa                                                >  1:908870/1‑150 (MQ=255)
                                                                                                        cgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGAt                                               >  1:1101619/1‑150 (MQ=255)
                                                                                                         gcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATa                                              <  1:132400/150‑1 (MQ=255)
                                                                                                         gcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTACGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATa                                              >  2:602386/1‑150 (MQ=255)
                                                                                                           aTTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaa                                            <  1:1639406/150‑1 (MQ=255)
                                                                                                            ttAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaa                                           <  1:575910/150‑1 (MQ=255)
                                                                                                              aaTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaaaa                                         >  2:916027/1‑150 (MQ=255)
                                                                                                              aaTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaaaa                                         >  1:856418/1‑150 (MQ=255)
                                                                                                               aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                        <  2:856418/150‑1 (MQ=255)
                                                                                                               aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                        <  1:495678/150‑1 (MQ=255)
                                                                                                                tCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAGGTAAGGATAAAAAAggg                                      >  2:1250508/1‑150 (MQ=255)
                                                                                                                  tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                     <  2:1101619/150‑1 (MQ=255)
                                                                                                                  tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                     >  1:978439/1‑150 (MQ=255)
                                                                                                                  tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                     >  2:1337443/1‑150 (MQ=255)
                                                                                                                     ttGTTAGTTAGGTTTGTCAAGTCCGTAATCTCGATAGAGGTTACGTACTTTTTGTATATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtgct                                  <  1:658844/150‑1 (MQ=255)
                                                                                                                       gTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtgctgc                                <  2:1087083/150‑1 (MQ=255)
                                                                                                                          aggtagggTGGTCAAGTCCGTAATCTCGAAATAGGTTACGGACTTTTTGTTTATGGGGGGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTGGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAgg                             <  1:1312402/150‑1 (MQ=255)
                                                                                                                            gtaggtTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTTGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGgat                           >  1:160477/1‑149 (MQ=255)
                                                                                                                              aggtTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGaa                         <  2:1541728/150‑1 (MQ=255)
                                                                                                                                 aacaaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                      >  2:1569607/7‑150 (MQ=38)
                                                                                                                                  tGGTCAAGTCCGTAATCTCGATAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATggg                     >  1:273603/1‑150 (MQ=255)
                                                                                                                                  acaaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATggg                     >  1:628994/6‑150 (MQ=38)
                                                                                                                                   ggTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTCCAGGAGAATGGGa                    >  2:297347/1‑150 (MQ=255)
                                                                                                                                   ggTAAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTCTGGGGGGGAGGCGGCTCAGACCCTTTTTTTAATGTTGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGa                    <  1:1534367/150‑1 (MQ=255)
                                                                                                                                     tCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATg                  <  1:1485252/150‑1 (MQ=255)
                                                                                                                                      aaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                      <  2:231913/144‑1 (MQ=255)
                                                                                                                                      aaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                      >  1:231913/2‑145 (MQ=255)
                                                                                                                                       aaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTATGTTGTTGATTATTAGTGCTGAGGGATGGTAAGGATAAAAAAGGGTGCAGCAGGAGAATGGGATGGt                >  2:269208/1‑150 (MQ=255)
                                                                                                                                         gTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTCTGGGGTGGAGGAGGTTCAGACCCTTTTTTTTATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt              <  1:1254280/150‑1 (MQ=255)
                                                                                                                                         gTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt              >  2:1582284/1‑150 (MQ=255)
                                                                                                                                         gTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt              >  2:1227959/1‑150 (MQ=255)
                                                                                                                                           ccGTAATCTCGAAAGAGGGTACGGACTTTTTGTTTATGGGGTGGAGGAGGTGCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTg            <  1:1358676/150‑1 (MQ=255)
                                                                                                                                                  ctcGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTttatt     >  1:824587/1‑150 (MQ=255)
                                                                                                                                                   tcGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTttatta    <  1:1017341/150‑1 (MQ=255)
                                                                                                                                                     gAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTaac  <  2:377981/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAAC  >  NC_000913/925429‑925727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: