Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 925,580 A→T 11.8% intergenic (+40/+304) clpA → / ← serW ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913925,5800AT11.8% 284.8 / 22.7 116intergenic (+40/+304)clpA/serWATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (43/59);  new base T (8/6);  total (51/65)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-01

Only 100 of 119 total aligned reads displayed.

GTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACA  >  NC_000913/925431‑925728
                                                                                                                                                     |                                                                                                                                                    
gTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGaa                                                                                                                                                      >  2:432950/1‑150 (MQ=255)
gTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGaa                                                                                                                                                      <  1:1235285/150‑1 (MQ=255)
    gCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAagag                                                                                                                                                  <  2:374515/150‑1 (MQ=255)
      tggtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGt                                                                                                                                                <  2:255906/150‑1 (MQ=255)
        gtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTa                                                                                                                                              <  2:1009766/150‑1 (MQ=255)
         tggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTACACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                             <  1:332081/150‑1 (MQ=255)
         tggACGGCGGTCAGGTCACCGGCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                             <  1:719517/150‑1 (MQ=255)
         gggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                             <  2:1512778/149‑1 (MQ=255)
          ggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACg                                                                                                                                            <  1:1322825/150‑1 (MQ=255)
           gACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACgg                                                                                                                                           <  2:332224/150‑1 (MQ=255)
           gACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACgg                                                                                                                                           >  1:1279752/1‑150 (MQ=255)
            aCGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACATACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGa                                                                                                                                          <  2:96777/150‑1 (MQ=255)
                 ggtcaggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACtttt                                                                                                                                     <  2:63411/150‑1 (MQ=255)
                    caggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                  <  1:1183120/150‑1 (MQ=255)
                      ggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGAc                                                                                                                                         <  2:1506927/141‑1 (MQ=255)
                      ggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGAc                                                                                                                                         >  1:1506927/1‑141 (MQ=255)
                       gtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTa                                                                                                                               >  1:1465670/1‑150 (MQ=255)
                         caCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                  >  2:1241074/1‑145 (MQ=255)
                         caCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                  <  1:1241074/145‑1 (MQ=255)
                          aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                            <  1:935002/150‑1 (MQ=255)
                          aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                            <  1:784324/150‑1 (MQ=255)
                          aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                            <  1:16647/150‑1 (MQ=255)
                                 cgcTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTggag                                                                                                                     >  1:629740/1‑150 (MQ=255)
                                  gcTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTggagg                                                                                                                    >  2:132400/1‑150 (MQ=255)
                                    tGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGgaggag                                                                                                                  >  1:120261/1‑150 (MQ=255)
                                     ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGaggagg                                                                                                                 >  2:286174/1‑150 (MQ=255)
                                     ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGGGGaggagg                                                                                                                 <  1:631195/150‑1 (MQ=255)
                                     ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGCCAAGTCCGTAATCTCGAATGAGGTTACGGACTTTTTGTTTATGGGGGGGaggagg                                                                                                                 <  2:516790/150‑1 (MQ=255)
                                        aaaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTc                                                                                                              <  1:1340177/149‑1 (MQ=255)
                                         aaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCa                                                                                                             <  2:1172108/150‑1 (MQ=255)
                                          aaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAg                                                                                                            >  1:1541728/1‑150 (MQ=255)
                                           agagAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGa                                                                                                           >  2:435591/1‑150 (MQ=255)
                                            tagaAAAATGCGCTGACTTACGGCTTCCAGAGTGCACAAACGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                                          <  2:805740/149‑1 (MQ=255)
                                            tagaAAAATGAGCTGACTTACGGATTCCAGAGTGAACAAAAGCACAGGGCTGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGGTCAGAc                                                                                                          <  2:1312603/149‑1 (MQ=255)
                                            gagaAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                                          <  1:1638528/150‑1 (MQ=255)
                                            gagaAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                                          <  2:609716/150‑1 (MQ=255)
                                                aaaaTGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGAGAATTAATCTGATTGTTAGGTAGGTTGGTCAATTCGTTAATCTAGAAAGATGTTACGGACTTTTTGTTTATGTGGTGGAGGAGGTTCAGAccata                                                                                                      >  2:33432/1‑147 (MQ=255)
                                                    tGAGCTGACTTACGGATTGCAGACTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCATGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTTGAGGAGGTTCAGACCCtttttt                                                                                                  <  2:1044757/150‑1 (MQ=255)
                                                     gAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGCCCCttttttt                                                                                                 >  2:1358676/1‑150 (MQ=255)
                                                       gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                               >  1:384064/1‑150 (MQ=255)
                                                       gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                               <  1:169897/150‑1 (MQ=255)
                                                       gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                               >  1:833392/1‑150 (MQ=255)
                                                        cTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAat                                                                                              <  2:1331859/150‑1 (MQ=255)
                                                          gACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTATGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatga                                                                                            <  1:763379/150‑1 (MQ=255)
                                                           aCTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgat                                                                                           <  2:1488576/150‑1 (MQ=255)
                                                            cTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatg                                                                                          >  2:1639406/1‑150 (MQ=255)
                                                             ttACGGATTCCAGAGTACACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatga                                                                                         <  1:873637/150‑1 (MQ=255)
                                                             ttACGGATTCCAGAGGGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatga                                                                                         <  1:128066/150‑1 (MQ=255)
                                                                      ccAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATTATGGTAAgtt                                                                                >  2:1254280/1‑150 (MQ=255)
                                                                      ccAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAgtt                                                                                >  1:1146042/1‑150 (MQ=255)
                                                                        agagTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTATTTTAATGATGATGGTAAgttct                                                                              >  1:80490/1‑148 (MQ=255)
                                                                         gagTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAgttgtt                                                                             <  2:1248205/150‑1 (MQ=255)
                                                                          agagCAGAACAGCACAAGGCTGAAGCAGAGCATTAATCTGATTGTTAGGTTGGTTGGTCAAGTCCGTTATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAGCCTTTTTTTAATGATGATGGTAAGttgttg                                                                            <  1:260496/147‑1 (MQ=255)
                                                                              cacaAAAGCACAAGGCGGCAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAACCTTTTTTTAATGATGATGGTAAGTTGTTGATaa                                                                        >  1:241353/1‑150 (MQ=255)
                                                                              cacaAAAGCACAAGGCGGAAGCAGCGCATTTATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATaa                                                                        >  2:386890/1‑150 (MQ=255)
                                                                              cacaAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGAtta                                                                        >  2:801651/1‑148 (MQ=255)
                                                                               acaAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACTGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTTTTGATAAt                                                                       >  1:150037/1‑150 (MQ=255)
                                                                                  aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                    <  2:1525360/150‑1 (MQ=255)
                                                                                  aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                    <  1:1030672/150‑1 (MQ=255)
                                                                                  aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTGTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                    >  1:457076/1‑150 (MQ=255)
                                                                                  aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTACGTAATCTCGAAAGAGGTTACTGACTTTTTGTTTATGGTGTTGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                    <  1:238917/150‑1 (MQ=255)
                                                                                          aGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCgg                                                            <  2:1279752/150‑1 (MQ=255)
                                                                                              ggAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAg                                                        >  2:575910/1‑150 (MQ=255)
                                                                                                   catcgcATTAATGTTTTTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTTTTTATGGGGTGGAGGTGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAg                                                   <  2:1465670/147‑1 (MQ=255)
                                                                                                     gcgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGTGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGa                                                 >  1:914959/1‑150 (MQ=255)
                                                                                                     gcgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGa                                                 >  1:908870/1‑150 (MQ=255)
                                                                                                      cgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGAt                                                >  1:1101619/1‑150 (MQ=255)
                                                                                                       gcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATa                                               <  1:132400/150‑1 (MQ=255)
                                                                                                       gcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTACGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATa                                               >  2:602386/1‑150 (MQ=255)
                                                                                                         aTTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaa                                             <  1:1639406/150‑1 (MQ=255)
                                                                                                          ttAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaa                                            <  1:575910/150‑1 (MQ=255)
                                                                                                            aaTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaaaa                                          >  2:916027/1‑150 (MQ=255)
                                                                                                            aaTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaaaa                                          >  1:856418/1‑150 (MQ=255)
                                                                                                             aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                         <  2:856418/150‑1 (MQ=255)
                                                                                                             aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                         <  1:495678/150‑1 (MQ=255)
                                                                                                             aTCTGATTGTTACGTAGGTTGGTGAAGTCCGTAATGTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATTATTGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                         <  1:154779/150‑1 (MQ=255)
                                                                                                              tCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAGGTAAGGATAAAAAAggg                                       >  2:1250508/1‑150 (MQ=255)
                                                                                                                tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                      <  2:1101619/150‑1 (MQ=255)
                                                                                                                tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                      >  2:1337443/1‑150 (MQ=255)
                                                                                                                tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                      >  1:978439/1‑150 (MQ=255)
                                                                                                                   ttGTTAGTTAGGTTTGTCAAGTCCGTAATCTCGATAGAGGTTACGTACTTTTTGTATATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtgct                                   <  1:658844/150‑1 (MQ=255)
                                                                                                                     gTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtgctgc                                 <  2:1087083/150‑1 (MQ=255)
                                                                                                                          gtaggtTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTTGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGgat                            >  1:160477/1‑149 (MQ=255)
                                                                                                                            aggtTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGaa                          <  2:1541728/150‑1 (MQ=255)
                                                                                                                               aacaaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                       >  2:1569607/7‑150 (MQ=38)
                                                                                                                                tGGTCAAGTCCGTAATCTCGATAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATggg                      >  1:273603/1‑150 (MQ=255)
                                                                                                                                acaaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATggg                      >  1:628994/6‑150 (MQ=38)
                                                                                                                                 ggTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTCCAGGAGAATGGGa                     >  2:297347/1‑150 (MQ=255)
                                                                                                                                 ggTAAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTCTGGGGGGGAGGCGGCTCAGACCCTTTTTTTAATGTTGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGa                     <  1:1534367/150‑1 (MQ=255)
                                                                                                                                   tCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATg                   <  1:1485252/150‑1 (MQ=255)
                                                                                                                                    aaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                       >  1:231913/2‑145 (MQ=255)
                                                                                                                                    aaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                       <  2:231913/144‑1 (MQ=255)
                                                                                                                                     aaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTATGTTGTTGATTATTAGTGCTGAGGGATGGTAAGGATAAAAAAGGGTGCAGCAGGAGAATGGGATGGt                 >  2:269208/1‑150 (MQ=255)
                                                                                                                                       gTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTCTGGGGTGGAGGAGGTTCAGACCCTTTTTTTTATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt               <  1:1254280/150‑1 (MQ=255)
                                                                                                                                       gTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt               >  2:1582284/1‑150 (MQ=255)
                                                                                                                                       gTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt               >  2:1227959/1‑150 (MQ=255)
                                                                                                                                         ccGTAATCTCGAAAGAGGGTACGGACTTTTTGTTTATGGGGTGGAGGAGGTGCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTg             <  1:1358676/150‑1 (MQ=255)
                                                                                                                                                ctcGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTttatt      >  1:824587/1‑150 (MQ=255)
                                                                                                                                                 tcGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTttatta     <  1:1017341/150‑1 (MQ=255)
                                                                                                                                                   gAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTaac   <  2:377981/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACA  >  NC_000913/925431‑925728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: