Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 925,585 G→A 10.3% intergenic (+45/+299) clpA → / ← serW ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913925,5850GA10.3% 333.8 / 19.4 116intergenic (+45/+299)clpA/serWATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base G (44/60);  new base A (7/5);  total (51/65)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.78e-02

Only 100 of 118 total aligned reads displayed.

CTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGC  >  NC_000913/925436‑925734
                                                                                                                                                     |                                                                                                                                                     
cTGGTGGACGGCGGTCAGGTCACCGTCGCGCAGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTATTCTCCAAAGAgg                                                                                                                                                       >  2:129504/1‑150 (MQ=255)
 tggtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGt                                                                                                                                                      <  2:255906/150‑1 (MQ=255)
   gtggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTa                                                                                                                                                    <  2:1009766/150‑1 (MQ=255)
    tggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTACACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                                   <  1:332081/150‑1 (MQ=255)
    tggACGGCGGTCAGGTCACCGGCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                                   <  1:719517/150‑1 (MQ=255)
    gggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTAc                                                                                                                                                   <  2:1512778/149‑1 (MQ=255)
     ggACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACg                                                                                                                                                  <  1:1322825/150‑1 (MQ=255)
      gACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACgg                                                                                                                                                 <  2:332224/150‑1 (MQ=255)
      gACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACgg                                                                                                                                                 >  1:1279752/1‑150 (MQ=255)
       aCGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGa                                                                                                                                                <  1:1468181/150‑1 (MQ=255)
       aCGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACATACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGa                                                                                                                                                <  2:96777/150‑1 (MQ=255)
           cggTCAGGACACCGTCGCGCTGGATAAAGACCAAACTGAGCTGACTTACGGATGCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGACttg                                                                                                                                            <  1:98995/150‑2 (MQ=255)
            ggtcaggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACtttt                                                                                                                                           <  2:63411/150‑1 (MQ=255)
               caggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                        <  1:1183120/150‑1 (MQ=255)
                 ggtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAACCTCTTTCGAGATTACGGAc                                                                                                                                               >  1:1506927/1‑141 (MQ=255)
                  gtcaCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTa                                                                                                                                     >  1:1465670/1‑150 (MQ=255)
                    caCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                        <  1:1241074/145‑1 (MQ=255)
                    caCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGt                                                                                                                                        >  2:1241074/1‑145 (MQ=255)
                     aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                                  <  1:784324/150‑1 (MQ=255)
                     aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                                  <  1:16647/150‑1 (MQ=255)
                     aCCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATgg                                                                                                                                  <  1:935002/150‑1 (MQ=255)
                               tgtaTAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTTATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGgaggag                                                                                                                        <  2:141480/147‑1 (MQ=255)
                               tGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGgaggag                                                                                                                        >  1:120261/1‑150 (MQ=255)
                                ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGaggagg                                                                                                                       >  2:286174/1‑150 (MQ=255)
                                ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGaggagg                                                                                                                       >  2:495678/1‑150 (MQ=255)
                                ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGGGGaggagg                                                                                                                       <  1:631195/150‑1 (MQ=255)
                                ggATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGCCAAGTCCGTAATCTCGAATGAGGTTACGGACTTTTTGTTTATGGGGGGGaggagg                                                                                                                       <  2:516790/150‑1 (MQ=255)
                                   aaaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTc                                                                                                                    <  1:1340177/149‑1 (MQ=255)
                                    aaaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCa                                                                                                                   <  2:1172108/150‑1 (MQ=255)
                                     aaGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAg                                                                                                                  >  1:1541728/1‑150 (MQ=255)
                                       tagaAAAATGCGCTGACTTACGGCTTCCAGAGTGCACAAACGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                                                <  2:805740/149‑1 (MQ=255)
                                       tagaAAAATGAGCTGACTTACGGATTCCAGAGTGAACAAAAGCACAGGGCTGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGGTCAGAc                                                                                                                <  2:1312603/149‑1 (MQ=255)
                                       gagaAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAc                                                                                                                <  2:609716/150‑1 (MQ=255)
                                           aaaaTGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGAGAATTAATCTGATTGTTAGGTAGGTTGGTCAATTCGTTAATCTAGAAAGATGTTACGGACTTTTTGTTTATGTGGTGGAGGAGGTTCAGAccata                                                                                                            >  2:33432/1‑147 (MQ=255)
                                                gAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGCCCCttttttt                                                                                                       >  2:1358676/1‑150 (MQ=255)
                                                gAGCTGACTTACGGATTCCAGAGAGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCttttttt                                                                                                       <  2:120261/150‑1 (MQ=255)
                                                  gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                                     >  1:384064/1‑150 (MQ=255)
                                                  gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                                     >  1:833392/1‑150 (MQ=255)
                                                  gCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTaa                                                                                                     <  1:169897/150‑1 (MQ=255)
                                                   cTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAat                                                                                                    <  2:1331859/150‑1 (MQ=255)
                                                     gACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTATGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatga                                                                                                  <  1:763379/150‑1 (MQ=255)
                                                      aCTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgat                                                                                                 <  2:1488576/150‑1 (MQ=255)
                                                       cTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatg                                                                                                >  2:1639406/1‑150 (MQ=255)
                                                        ttACGGATTCCAGAGGGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAatgatga                                                                                               <  1:128066/150‑1 (MQ=255)
                                                                 ccAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATTATGGTAAgtt                                                                                      >  2:1254280/1‑150 (MQ=255)
                                                                 ccAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAgtt                                                                                      >  1:1146042/1‑150 (MQ=255)
                                                                     agagCAGAACAGCACAAGGCTGAAGCAGAGCATTAATCTGATTGTTAGGTTGGTTGGTCAAGTCCGTTATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAGCCTTTTTTTAATGATGATGGTAAGttgttg                                                                                  <  1:260496/147‑1 (MQ=255)
                                                                         cacaAAAGCACAAGGCGGCAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGAACCTTTTTTTAATGATGATGGTAAGTTGTTGATaa                                                                              >  1:241353/1‑150 (MQ=255)
                                                                         cacaAAAGCACAAGGCGGAAGCAGCGCATTTATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATaa                                                                              >  2:386890/1‑150 (MQ=255)
                                                                         cacaAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGAtta                                                                              >  2:801651/1‑148 (MQ=255)
                                                                             aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                          <  2:1525360/150‑1 (MQ=255)
                                                                             aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                          <  2:358600/150‑1 (MQ=255)
                                                                             aaaGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAg                                                                          <  1:1030672/150‑1 (MQ=255)
                                                                               aGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGtg                                                                        >  1:864404/1‑150 (MQ=255)
                                                                                gCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTAACGGACTATTTGTTTATGGGGTGGAGGAGGTTCAAACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGtgc                                                                       >  2:1534367/1‑150 (MQ=255)
                                                                                     aGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCgg                                                                  <  2:1279752/150‑1 (MQ=255)
                                                                                         ggAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAg                                                              >  2:575910/1‑150 (MQ=255)
                                                                                              catcgcATTAATGTTTTTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTTTTTATGGGGTGGAGGTGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAg                                                         <  2:1465670/147‑1 (MQ=255)
                                                                                                gcgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGTGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGa                                                       >  1:914959/1‑150 (MQ=255)
                                                                                                gcgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGa                                                       >  1:908870/1‑150 (MQ=255)
                                                                                                 cgcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGAt                                                      >  1:1101619/1‑150 (MQ=255)
                                                                                                 cgcATTAATCTGATTGTTAGGTAGGTTGGGCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGAt                                                      <  1:778997/150‑1 (MQ=255)
                                                                                                  gcATTAATCTGATTGTTAGGTAGGTTGGTCAAGTACGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATa                                                     >  2:602386/1‑150 (MQ=255)
                                                                                                    aTTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaa                                                   <  1:1639406/150‑1 (MQ=255)
                                                                                                     ttAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaa                                                  <  1:575910/150‑1 (MQ=255)
                                                                                                       aaTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaaaa                                                >  2:916027/1‑150 (MQ=255)
                                                                                                       aaTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATaaaaaa                                                >  1:856418/1‑150 (MQ=255)
                                                                                                        aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                               <  2:856418/150‑1 (MQ=255)
                                                                                                        aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                               <  1:286174/150‑1 (MQ=255)
                                                                                                        aTCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                               <  1:495678/150‑1 (MQ=255)
                                                                                                        aTCTGATTGTTACGTAGGTTGGTGAAGTCCGTAATGTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATTATTGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAg                                               <  1:154779/150‑1 (MQ=255)
                                                                                                         tCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAGGTAAGGATAAAAAAggg                                             >  2:1250508/1‑150 (MQ=255)
                                                                                                           tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                            >  1:978439/1‑150 (MQ=255)
                                                                                                           tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                            >  2:1337443/1‑150 (MQ=255)
                                                                                                           tGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGt                                            <  2:1101619/150‑1 (MQ=255)
                                                                                                            gATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtg                                           >  1:1700807/1‑150 (MQ=255)
                                                                                                              ttGTTAGTTAGGTTTGTCAAGTCCGTAATCTCGATAGAGGTTACGTACTTTTTGTATATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtgct                                         <  1:658844/150‑1 (MQ=255)
                                                                                                                gTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGtgctgc                                       <  2:1087083/150‑1 (MQ=255)
                                                                                                                   aggtaggtTGGTCAAGTCCATAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAgg                                    <  1:58775/150‑1 (MQ=255)
                                                                                                                   aggtagggTGGTCAAGTCCGTAATCTCGAAATAGGTTACGGACTTTTTGTTTATGGGGGGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTGGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAgg                                    <  1:1312402/150‑1 (MQ=255)
                                                                                                                     gtaggtTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTTGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGgat                                  >  1:160477/1‑149 (MQ=255)
                                                                                                                       aggtTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGaa                                <  2:1541728/150‑1 (MQ=255)
                                                                                                                          aacaaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                             >  2:1569607/7‑150 (MQ=38)
                                                                                                                           tGGTCAAGTCCGTAATCTCGATAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATggg                            >  1:273603/1‑150 (MQ=255)
                                                                                                                           acaaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATggg                            >  1:628994/6‑150 (MQ=38)
                                                                                                                            ggTAAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTCTGGGGGGGAGGCGGCTCAGACCCTTTTTTTAATGTTGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGa                           <  1:1534367/150‑1 (MQ=255)
                                                                                                                             aaaaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGTGAAGGTAAGGATAAAAAAGGGTTCTGCAGGAGAATGGGAt                          >  2:253000/4‑150 (MQ=255)
                                                                                                                              tCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATg                         <  1:1485252/150‑1 (MQ=255)
                                                                                                                               aaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                             >  1:231913/2‑145 (MQ=255)
                                                                                                                               aaaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATgg                             <  2:231913/144‑1 (MQ=255)
                                                                                                                                aaGTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTATGTTGTTGATTATTAGTGCTGAGGGATGGTAAGGATAAAAAAGGGTGCAGCAGGAGAATGGGATGGt                       >  2:269208/1‑150 (MQ=255)
                                                                                                                                  gTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTCTGGGGTGGAGGAGGTTCAGACCCTTTTTTTTATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt                     <  1:1254280/150‑1 (MQ=255)
                                                                                                                                  gTCCGTAACCTCTTTCGAGATTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGttt                     >  2:1227959/1‑150 (MQ=255)
                                                                                                                                    ccGTAATCTCGAAAGAGGGTACGGACTTTTTGTTTATGGGGTGGAGGAGGTGCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTg                   <  1:1358676/150‑1 (MQ=255)
                                                                                                                                           ctcGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTttatt            >  1:824587/1‑150 (MQ=255)
                                                                                                                                            tcGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTttatta           <  1:1017341/150‑1 (MQ=255)
                                                                                                                                              gAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTaac         <  2:377981/150‑1 (MQ=255)
                                                                                                                                               aaaGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGATGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTaaca        <  1:1582284/150‑1 (MQ=255)
                                                                                                                                                  gagGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACg     >  1:863850/1‑150 (MQ=255)
                                                                                                                                                     gTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGc  <  1:491021/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCTGACTTACGGATTCCAGAGTGCACAAAAGCACAAGGCGGAAGCAGCGCATTAATCTGATTGTTAGGTAGGTTGGTCAAGTCCGTAATCTCGAAAGAGGTTACGGACTTTTTGTTTATGGGGTGGAGGAGGTTCAGACCCTTTTTTTAATGATGATGGTAAGTTGTTGATAATTAGTGCTGCGGGAAGGTAAGGATAAAAAAGGGTGCTGCAGGAGAATGGGATGGTTTTGCTTTATTAACAACGGGC  >  NC_000913/925436‑925734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: