Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 950,313 C→T 8.0% intergenic (+55/+27) ycaK → / ← pflA putative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913950,3130CT 8.0% 376.8 / 14.8 125intergenic (+55/+27)ycaK/pflAputative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase
Reads supporting (aligned to +/- strand):  ref base C (58/55);  new base T (6/4);  total (65/60)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 127 total aligned reads displayed.

TGTGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGT  >  NC_000913/950164‑950460
                                                                                                                                                     |                                                                                                                                                   
  tgtAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCaa                                                                                                                                                   >  2:146073/1‑150 (MQ=255)
      aTACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATaaa                                                                                                                                               >  2:207255/1‑150 (MQ=255)
      aTACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATaaa                                                                                                                                               >  2:1719742/1‑150 (MQ=255)
       tACAGTGGTATACGATGGGGAATAACTTCACGCGAGCTATTATCTGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAg                                                                                                                                              <  2:146123/150‑1 (MQ=255)
        aCAGTGGTATTTGATGTTGAATAACTTCACGCTTGCTATTATCAGTCTTTATTATCTCAGGTACGGGAAATGGTAGATGCACTATAAGATTTGTTAATATCTCTGTAGCAGAATGAAGCGCGGATTAAAAAAGCGGCAACTCAATTAAGt                                                                                                                                             <  1:125034/150‑1 (MQ=255)
        aCAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGt                                                                                                                                             <  2:1497310/150‑1 (MQ=255)
         cAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGtt                                                                                                                                            >  2:1112177/1‑150 (MQ=255)
          aGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTg                                                                                                                                           <  2:1405371/150‑1 (MQ=255)
          aGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTg                                                                                                                                           <  1:1036871/150‑1 (MQ=255)
           gTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGc                                                                                                                                          >  1:794538/1‑150 (MQ=255)
           gTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGc                                                                                                                                          >  1:1515106/1‑150 (MQ=255)
           gTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGc                                                                                                                                          <  1:140953/150‑1 (MQ=255)
           gTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGc                                                                                                                                          <  1:146073/150‑1 (MQ=255)
            tGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGcc                                                                                                                                         <  2:655610/150‑1 (MQ=255)
              ttATTCGATGGGGAAGAACTTCACGCGAGCTTTTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGc                                                                                                                                       <  2:1465584/149‑1 (MQ=255)
              gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc                                                                                                                                       <  1:187511/150‑1 (MQ=255)
              gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGc                                                                                                                                       >  2:1274238/1‑150 (MQ=255)
               tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAATGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCt                                                                                                                                      <  1:187138/150‑1 (MQ=255)
               tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGCt                                                                                                                                      <  2:794538/150‑1 (MQ=255)
               tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGCt                                                                                                                                      <  2:1515106/150‑1 (MQ=255)
                    gATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACg                                                                                                                                 >  2:272110/1‑150 (MQ=255)
                     attgggAAGAACTTCACGCGAGCTATACTCAGTCGTTATTATCTCAGGAACGTGATATGGTAGATGCACTAAAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACgg                                                                                                                                <  2:450428/147‑1 (MQ=255)
                     aTGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACgg                                                                                                                                <  1:507050/150‑1 (MQ=255)
                     aTGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACgg                                                                                                                                >  1:1036566/1‑150 (MQ=255)
                            agaaCTTCTCGCGAGCTATTATCAGTCGTTATAATATCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAAtt                                                                                                                         <  2:192481/150‑1 (MQ=255)
                            agaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAAtt                                                                                                                         <  2:808771/150‑1 (MQ=255)
                             gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGcc                                                                                                                                         <  1:935871/133‑1 (MQ=255)
                                     cgcgAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAAT‑‑‑AAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGTGAAATTAGAACATTACCt                                                                                                             >  2:367470/1‑150 (MQ=255)
                                          gCTATTATCAGTCGTTATTATATCAGGTACGGGATATTGTATATGCACTATAAGATGTGTTAAAAACTCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCTGCAACTAAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTa                                                                                                           <  2:620832/150‑1 (MQ=255)
                                                       gTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCt                                                                                              <  2:415109/150‑1 (MQ=255)
                                                         tattatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCa                                                                                            >  2:469769/1‑150 (MQ=255)
                                                         tattagCTCCTGTACGGGATTTGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGCACCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCa                                                                                            <  2:163725/150‑1 (MQ=255)
                                                             atCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGaa                                                                                        <  1:362913/150‑1 (MQ=255)
                                                               ctcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATg                                                                                      >  2:148133/1‑150 (MQ=255)
                                                                   ggTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCtt                                                                                  <  1:1087326/150‑1 (MQ=255)
                                                                       cGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCAGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGATCAAGAATGCCTTTCAc                                                                              >  1:847884/1‑150 (MQ=255)
                                                                        gggATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCAcg                                                                             <  1:1307553/150‑1 (MQ=255)
                                                                         ggATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCTGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCActc                                                                            >  1:110236/1‑148 (MQ=255)
                                                                              tGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTcc                                                                       >  2:264537/1‑150 (MQ=255)
                                                                                gTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGTGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCAt                                                                     <  1:272110/150‑1 (MQ=255)
                                                                                 tAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATg                                                                    <  1:530310/150‑1 (MQ=255)
                                                                                  aGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATgg                                                                   <  1:583421/150‑1 (MQ=255)
                                                                                  aGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATgg                                                                   >  1:732524/1‑150 (MQ=255)
                                                                                     tGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGtct                                                                <  1:307030/150‑1 (MQ=255)
                                                                                        aCTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctt                                                             <  2:618995/150‑1 (MQ=255)
                                                                                        aCTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctt                                                             <  2:732524/150‑1 (MQ=255)
                                                                                         cTATAAGATGTGTTAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtcttt                                                            <  1:566857/150‑1 (MQ=255)
                                                                                         cTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACTCGTTCCATGGTCtcttt                                                            >  2:116567/1‑150 (MQ=255)
                                                                                           ataAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATATAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctttct                                                          <  1:264537/150‑1 (MQ=255)
                                                                                            taAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctttctt                                                         >  1:582267/1‑150 (MQ=255)
                                                                                            taAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctttctt                                                         >  2:802027/1‑150 (MQ=255)
                                                                                             aaGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTttcttc                                                        <  2:796721/150‑1 (MQ=255)
                                                                                              aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                       >  2:1425021/1‑150 (MQ=255)
                                                                                              aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                       >  2:456884/1‑150 (MQ=255)
                                                                                               gATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCATGAATGACTTTCACGCGTTCCATGGTCTCTTTCTTCgg                                                      >  2:900879/1‑150 (MQ=255)
                                                                                                aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                     <  1:1462557/150‑1 (MQ=255)
                                                                                                aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAAT‑‑‑AAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtggt                                                  <  1:367470/150‑1 (MQ=255)
                                                                                                  gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg                                                   >  2:1592830/1‑150 (MQ=255)
                                                                                                  gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg                                                   <  2:1531359/150‑1 (MQ=255)
                                                                                                  gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg                                                   <  2:1221478/150‑1 (MQ=255)
                                                                                                  gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg                                                   <  2:1372751/150‑1 (MQ=255)
                                                                                                    gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt                                                 >  1:967336/1‑150 (MQ=255)
                                                                                                    gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt                                                 >  2:864795/1‑150 (MQ=255)
                                                                                                    gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt                                                 <  2:61137/150‑1 (MQ=255)
                                                                                                     ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                <  1:1274238/150‑1 (MQ=255)
                                                                                                       aaaaaCGCTTTAGCACAATGAAGCGCGGAATAAAAAAGCGACAACTCAATAAAGATGCAGCTTTAAGGTGTAATTAGAACATTACCTTATGACCGAACTGCTCAATAATGCATTTCACGCGTTCGATGGTCTCTTTCTTCGGAGGTTTaa                                              >  2:371518/1‑150 (MQ=255)
                                                                                                        aaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAac                                             >  1:40631/1‑150 (MQ=255)
                                                                                                        aaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAac                                             >  2:1378943/1‑150 (MQ=255)
                                                                                                        aaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCATTCCGGGGAAATTAGCACATTACCTGATGACCGTACTGCTCACGAATCCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTatc                                             >  1:253953/1‑148 (MQ=255)
                                                                                                         aaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAaca                                            >  1:1503340/1‑150 (MQ=255)
                                                                                                           aCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACAcc                                          >  2:1323369/1‑150 (MQ=255)
                                                                                                            cGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCCCGCGTTCCGTGGTCTCTTTCTTCGGTGGTTTAACACCg                                         >  1:1171697/1‑150 (MQ=255)
                                                                                                            cGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCg                                         <  1:207255/150‑1 (MQ=255)
                                                                                                            cGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCg                                         <  1:1719742/150‑1 (MQ=255)
                                                                                                                gTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATTAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTTATGCTCAAGAATGCGTTTCACGCGGTCCATGGTCTCTTTCTTCGGTGGTTTTACACAGTCGa                                     >  2:762220/1‑150 (MQ=255)
                                                                                                                  aGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGt                                   >  2:1302467/1‑150 (MQ=255)
                                                                                                                   gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt                                  <  2:1466418/150‑1 (MQ=255)
                                                                                                                   gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt                                  <  1:861979/150‑1 (MQ=255)
                                                                                                                    cAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGttt                                 <  2:511837/150‑1 (MQ=255)
                                                                                                                     aGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTg                                >  1:1556416/1‑150 (MQ=255)
                                                                                                                         tGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTAct                            <  2:1413155/150‑1 (MQ=255)
                                                                                                                           aaGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTActct                          <  2:1632970/150‑1 (MQ=255)
                                                                                                                              cgcgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCa                       >  1:165868/1‑150 (MQ=255)
                                                                                                                               gcgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCAc                      <  1:148133/150‑1 (MQ=255)
                                                                                                                                cgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAAGACCGTCGAGTTTGTTATCTTCAcc                     >  2:244564/1‑150 (MQ=255)
                                                                                                                                  gAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCa                   >  2:1512658/1‑150 (MQ=255)
                                                                                                                                    aTAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCAtt                 >  1:149821/1‑150 (MQ=255)
                                                                                                                                       aaaaaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGcc              <  1:1112177/150‑1 (MQ=255)
                                                                                                                                         aaaGCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAc            >  1:709603/1‑150 (MQ=255)
                                                                                                                                           aGCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTACCACCGTCGAGTTTGTACTCTTCACCCATTGCCAccc          >  2:268317/1‑150 (MQ=255)
                                                                                                                                           aGCGGCAACTTTATTGAGTTGCCGCTTTACGGGAAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTTTACTCTTCACCCATTGCCAccc          >  2:1577821/1‑150 (MQ=255)
                                                                                                                                           aGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAccc          >  1:461496/1‑150 (MQ=255)
                                                                                                                                            gCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTAAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCa         >  1:1011479/1‑150 (MQ=255)
                                                                                                                                            gCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATTCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCa         >  2:686454/1‑150 (MQ=255)
                                                                                                                                             cGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCAt        >  2:914637/1‑150 (MQ=255)
                                                                                                                                             cGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCAt        >  2:1083659/1‑150 (MQ=255)
                                                                                                                                              ggCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAAATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACAGTCGAGTATGTACTCTTCACACATTGCAACCCAtt       >  1:1025714/1‑150 (MQ=255)
                                                                                                                                                  aCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgtg   <  2:398697/150‑1 (MQ=255)
                                                                                                                                                  aCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCGTCACCCATTGCCACCCATTtgtg   >  2:1390512/1‑150 (MQ=255)
                                                                                                                                                   cTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCAATTTgtgt  >  1:487657/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGTGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGT  >  NC_000913/950164‑950460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: