Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 950,314 | A→T | 8.1% | intergenic (+56/+26) | ycaK → / ← pflA | putative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 950,314 | 0 | A | T | 8.1% | 341.1 / 15.0 | 123 | intergenic (+56/+26) | ycaK/pflA | putative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase |
Reads supporting (aligned to +/- strand): ref base A (57/56); new base T (6/4); total (63/60) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.44e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 |
Only 100 of 125 total aligned reads displayed.
TGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGT > NC_000913/950166‑950460 | tgtAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCaa > 2:146073/1‑150 (MQ=255) tAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGAAGAATGAAGCGCGGAATAAAAAAGCTGAAACTAAATa > 1:192481/1‑150 (MQ=255) aTACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATaaa > 2:207255/1‑150 (MQ=255) tACAGTGGTATACGATGGGGAATAACTTCACGCGAGCTATTATCTGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAg < 2:146123/150‑1 (MQ=255) aCAGTGGTATTTGATGTTGAATAACTTCACGCTTGCTATTATCAGTCTTTATTATCTCAGGTACGGGAAATGGTAGATGCACTATAAGATTTGTTAATATCTCTGTAGCAGAATGAAGCGCGGATTAAAAAAGCGGCAACTCAATTAAGt < 1:125034/150‑1 (MQ=255) cAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGtt > 2:1112177/1‑150 (MQ=255) aGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTg < 1:1036871/150‑1 (MQ=255) aGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTg < 2:1405371/150‑1 (MQ=255) gTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGc > 1:794538/1‑150 (MQ=255) gTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGc > 1:1515106/1‑150 (MQ=255) gTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGc < 1:140953/150‑1 (MQ=255) gTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGc < 1:146073/150‑1 (MQ=255) tGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGcc < 2:655610/150‑1 (MQ=255) ttATTCGATGGGGAAGAACTTCACGCGAGCTTTTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGc < 2:1465584/149‑1 (MQ=255) gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc < 1:187511/150‑1 (MQ=255) gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGc > 2:1274238/1‑150 (MQ=255) tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAATGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCt < 1:187138/150‑1 (MQ=255) tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGCt < 2:1515106/150‑1 (MQ=255) tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGCt < 2:794538/150‑1 (MQ=255) tCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGCtttt < 2:103932/150‑2 (MQ=255) cGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTAc > 2:1647760/1‑150 (MQ=255) gATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACg > 2:272110/1‑150 (MQ=255) attgggAAGAACTTCACGCGAGCTATACTCAGTCGTTATTATCTCAGGAACGTGATATGGTAGATGCACTAAAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACgg < 2:450428/147‑1 (MQ=255) aTGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACgg > 1:1036566/1‑150 (MQ=255) aTGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACgg < 1:507050/150‑1 (MQ=255) aTGCGGAAGAACTGCACGCTAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGAAGCACTATAAGCTGTGTTAAAACCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACgg < 2:252991/150‑1 (MQ=255) ggAAGAACTTCACGCGAGCTATTATCAGTTGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGaa > 2:530310/1‑150 (MQ=255) gaagaaCTTCACGCGAGCTATTCTCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGaaa < 2:1569070/150‑1 (MQ=255) agaaCTTCTCGCGAGCTATTATCAGTCGTTATAATATCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAAtt < 2:192481/150‑1 (MQ=255) gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGcc < 1:935871/133‑1 (MQ=255) gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa > 1:398697/1‑150 (MQ=255) cTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATAAACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGaa > 2:861979/1‑150 (MQ=255) cgcgAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAAT‑‑‑AAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGTGAAATTAGAACATTACCt > 2:367470/1‑150 (MQ=255) aGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCtt > 2:42724/1‑150 (MQ=255) aGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTAc > 1:511837/1‑150 (MQ=255) gTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCt < 2:415109/150‑1 (MQ=255) tattatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCa > 2:1494820/1‑150 (MQ=255) tattagCTCCTGTACGGGATTTGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGCACCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCa < 2:163725/150‑1 (MQ=255) atCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGaa < 1:362913/150‑1 (MQ=255) ctcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATg > 2:148133/1‑150 (MQ=255) ggTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCtt < 1:1087326/150‑1 (MQ=255) cGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCAGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGATCAAGAATGCCTTTCAc > 1:847884/1‑150 (MQ=255) gggATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCAcg < 1:1307553/150‑1 (MQ=255) ggATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCTGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCActc > 1:110236/1‑148 (MQ=255) gTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGTGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCAt < 1:272110/150‑1 (MQ=255) tAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATg < 1:530310/150‑1 (MQ=255) aGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATgg < 1:583421/150‑1 (MQ=255) gCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGtctc < 1:270993/150‑1 (MQ=255) aCTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctt < 2:618995/150‑1 (MQ=255) aCTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctt < 2:732524/150‑1 (MQ=255) cTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACTCGTTCCATGGTCtcttt > 2:116567/1‑150 (MQ=255) ataAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATATAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctttct < 1:264537/150‑1 (MQ=255) taAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctttctt > 1:582267/1‑150 (MQ=255) taAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtctttctt > 2:802027/1‑150 (MQ=255) aaGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTttcttc < 2:796721/150‑1 (MQ=255) aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg > 2:456884/1‑150 (MQ=255) aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg > 2:1425021/1‑150 (MQ=255) gATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCATGAATGACTTTCACGCGTTCCATGGTCTCTTTCTTCgg > 2:900879/1‑150 (MQ=255) aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt < 1:1462557/150‑1 (MQ=255) aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAAT‑‑‑AAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtggt < 1:367470/150‑1 (MQ=255) gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg < 2:1372751/150‑1 (MQ=255) gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg > 2:1592830/1‑150 (MQ=255) gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg < 2:1531359/150‑1 (MQ=255) gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt < 2:61137/150‑1 (MQ=255) gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt > 2:864795/1‑150 (MQ=255) ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt < 1:1274238/150‑1 (MQ=255) tAAAAACGCTGTAGCAGTATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTa < 2:628864/150‑1 (MQ=255) aaaaaCGCTTTAGCACAATGAAGCGCGGAATAAAAAAGCGACAACTCAATAAAGATGCAGCTTTAAGGTGTAATTAGAACATTACCTTATGACCGAACTGCTCAATAATGCATTTCACGCGTTCGATGGTCTCTTTCTTCGGAGGTTTaa > 2:371518/1‑150 (MQ=255) aaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAac > 2:1378943/1‑150 (MQ=255) aaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAac > 1:40631/1‑150 (MQ=255) aaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCATTCCGGGGAAATTAGCACATTACCTGATGACCGTACTGCTCACGAATCCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTatc > 1:253953/1‑148 (MQ=255) aaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAaca > 1:1503340/1‑150 (MQ=255) aCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACAcc > 2:1323369/1‑150 (MQ=255) cGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCCCGCGTTCCGTGGTCTCTTTCTTCGGTGGTTTAACACCg > 1:1171697/1‑150 (MQ=255) cGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCg < 1:1719742/150‑1 (MQ=255) cGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCg > 2:1240571/1‑150 (MQ=255) tGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCg < 1:1647760/150‑1 (MQ=255) aGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGt > 2:1302467/1‑150 (MQ=255) aGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGt > 2:769733/1‑150 (MQ=255) gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt < 2:1466418/150‑1 (MQ=255) gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt < 1:861979/150‑1 (MQ=255) cAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGttt < 2:511837/150‑1 (MQ=255) aGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTg > 1:1556416/1‑150 (MQ=255) tGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTAct < 2:1413155/150‑1 (MQ=255) aaGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTActct < 1:1494820/150‑1 (MQ=255) aaGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTActct < 2:1632970/150‑1 (MQ=255) gcgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCAc < 1:148133/150‑1 (MQ=255) cgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAAGACCGTCGAGTTTGTTATCTTCAcc > 2:244564/1‑150 (MQ=255) gAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCa > 2:1512658/1‑150 (MQ=255) aaaaaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGcc < 1:1112177/150‑1 (MQ=255) aaaGCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAc > 1:709603/1‑150 (MQ=255) aGCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTACCACCGTCGAGTTTGTACTCTTCACCCATTGCCAccc > 2:268317/1‑150 (MQ=255) gCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTAAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCa > 1:1011479/1‑150 (MQ=255) gCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATTCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCa > 2:686454/1‑150 (MQ=255) cGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCAt > 2:914637/1‑150 (MQ=255) cGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCAt > 2:1083659/1‑150 (MQ=255) ggCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAAATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACAGTCGAGTATGTACTCTTCACACATTGCAACCCAtt > 1:1025714/1‑150 (MQ=255) aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgt < 1:609379/150‑1 (MQ=255) aCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCGTCACCCATTGCCACCCATTtgtg > 2:1390512/1‑150 (MQ=255) cTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCAATTTgtgt > 1:487657/1‑150 (MQ=255) | TGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGT > NC_000913/950166‑950460 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |