Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,026,539 T→A 10.7% intergenic (+14/+18) helD → / ← mgsA DNA helicase IV/methylglyoxal synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,026,5390TA10.7% 197.4 / 12.2 80intergenic (+14/+18)helD/mgsADNA helicase IV/methylglyoxal synthase
Reads supporting (aligned to +/- strand):  ref base T (35/36);  new base A (4/4);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

GAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACC  >  NC_000913/1026391‑1026686
                                                                                                                                                    |                                                                                                                                                   
gaagaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCTGTGGCGAGAAAACCGTAAGAAACAGGTGGCGtt                                                                                                                                                    <  1:293999/150‑1 (MQ=255)
 aagaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGttt                                                                                                                                                   >  2:234865/1‑150 (MQ=255)
  agaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTg                                                                                                                                                  <  1:868963/150‑1 (MQ=255)
  agaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTg                                                                                                                                                  <  1:1235259/150‑1 (MQ=255)
   gaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGc                                                                                                                                                 <  1:1194979/150‑1 (MQ=255)
   gaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGc                                                                                                                                                 <  1:348180/150‑1 (MQ=255)
   gaaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGc                                                                                                                                                 <  2:389647/150‑1 (MQ=255)
    aaCGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGcc                                                                                                                                                <  1:2847/150‑1 (MQ=255)
       ggcggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCTCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCAcc                                                                                                                                             <  2:1194207/150‑1 (MQ=255)
       ggcggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCAcc                                                                                                                                             <  1:1022888/150‑1 (MQ=255)
       ggcggTTAATGGACGGGGCGCTGACCCGGGCACGCCATCGGTTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCAcc                                                                                                                                             <  2:263824/150‑1 (MQ=255)
         cggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtg                                                                                                                                           <  1:158679/150‑1 (MQ=255)
          ggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAATAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCtgt                                                                                                                                          <  2:1157158/150‑1 (MQ=255)
          ggTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgt                                                                                                                                          <  1:1560731/150‑1 (MQ=255)
           gTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtt                                                                                                                                         >  1:1206677/1‑149 (MQ=255)
           gTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtt                                                                                                                                         <  2:1206677/150‑2 (MQ=255)
           gTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTCAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCtgtg                                                                                                                                         <  2:829368/150‑1 (MQ=255)
             tAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCAAACGCCACCtgtttc                                                                                                                                       <  2:1245911/150‑4 (MQ=255)
                 gTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAatat                                                                                                                                   >  2:1650005/1‑150 (MQ=255)
                     gTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACt                                                                                                                               >  2:814644/1‑150 (MQ=255)
                       ggCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTc                                                                                                                             <  1:939129/150‑1 (MQ=255)
                           cTGACCCGGGCACGCCATCGGGACTGGGCACTGAATACCCAAGAGAATCCCTCTCCCTTTGTGGAAATACAGAAAAATCTGGATGTGCCGGCGGCGAGAAAACCGTAAGAAACAGGTGGCGCTTGCCACCTGTGCAATATTACTTCAGAc                                                                                                                         <  1:315800/150‑1 (MQ=255)
                              aCCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGt                                                                                                                      >  2:1384834/1‑150 (MQ=255)
                                  gggCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCcgc                                                                                                                  >  2:808213/1‑150 (MQ=255)
                                       cGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGAt                                                                                                             >  2:356941/1‑150 (MQ=255)
                                        gCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATa                                                                                                            >  2:684529/1‑150 (MQ=255)
                                         ccATCGGGTATGGGCACTGTTTAACAATGATAATCCCTCTCCCTTTGTGGAATTACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGGAATAAACAGGTGGCGTTTGCCAGCTGTGCAATATTAATTAAGACGGTCCGCGATATaa                                                                                                           >  2:943446/1‑150 (MQ=255)
                                          cATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAAc                                                                                                          <  1:1485406/150‑1 (MQ=255)
                                               gggaTGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTCAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGAAATATTACTTCAGACGGTCCGCGAGATAACGCTGa                                                                                                     <  1:1073875/147‑1 (MQ=255)
                                               ggTATGTGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGa                                                                                                     <  1:469643/150‑1 (MQ=255)
                                                gTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGAt                                                                                                    <  2:1376473/150‑1 (MQ=255)
                                                    gggCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATc                                                                                                <  1:573831/150‑1 (MQ=255)
                                                      gCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCAACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCgg                                                                                              >  1:203636/1‑150 (MQ=255)
                                                               aaCAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGaa                                                                                     >  1:1538891/1‑150 (MQ=255)
                                                                aCAAATAGAAGCCCTCTCCCTTTGTGGAAATACTGCAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTTCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAat                                                                                    <  2:1077223/150‑1 (MQ=255)
                                                                 cAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAata                                                                                   >  2:1564189/1‑150 (MQ=255)
                                                                  aaaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAatat                                                                                  <  1:695239/150‑1 (MQ=255)
                                                                  aaaGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGAATCAGAatat                                                                                  >  1:78914/1‑150 (MQ=255)
                                                                       gaATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGccc                                                                             >  2:1267754/1‑150 (MQ=255)
                                                                         aTCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACcgc                                                                           >  1:1611796/1‑150 (MQ=255)
                                                                         aTCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACcgc                                                                           >  2:943278/1‑150 (MQ=255)
                                                                           cccTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgt                                                                         <  1:547699/150‑1 (MQ=255)
                                                                            ccTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAACCCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgtc                                                                        >  1:343286/1‑150 (MQ=255)
                                                                             ctctCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGcgtcg                                                                       <  1:808213/150‑1 (MQ=255)
                                                                                tcCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTg                                                                    >  1:803887/1‑150 (MQ=255)
                                                                                 cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                   >  2:604413/1‑150 (MQ=255)
                                                                                 cccTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGa                                                                   <  2:1140713/150‑1 (MQ=255)
                                                                                        tgGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATgcgg                                                            <  1:234865/150‑1 (MQ=255)
                                                                                         ggAAATACTGAAAAATCTGGATGTGCAGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCTGGGATCAGAATATCGACCGCGTCGTTGAAATgcggc                                                           >  2:1253918/1‑150 (MQ=255)
                                                                                             aTACTGAAAACTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACt                                                       <  2:60624/150‑1 (MQ=255)
                                                                                                cTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGa                                                    >  2:842375/1‑150 (MQ=255)
                                                                                                  taaaaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCTCCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATAGGGGAGCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                  <  1:790156/149‑1 (MQ=255)
                                                                                                  gAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGAtt                                                  >  2:1315732/1‑150 (MQ=255)
                                                                                                    aaaaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTAt                                                >  2:257563/1‑150 (MQ=255)
                                                                                                      aaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGa                                              <  1:547013/150‑1 (MQ=255)
                                                                                                      aaTCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGa                                              <  2:504562/150‑1 (MQ=255)
                                                                                                          tGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGta                                          >  2:590141/1‑149 (MQ=255)
                                                                                                           ggATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCt                                         >  2:631357/1‑150 (MQ=255)
                                                                                                            gATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCATTGAAATGCGGCAACTGGATTATGAAGTCTg                                        <  2:771203/150‑1 (MQ=255)
                                                                                                             aTGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGc                                       <  1:814644/150‑1 (MQ=255)
                                                                                                             aTGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGc                                       >  1:1068557/1‑150 (MQ=255)
                                                                                                              tgtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCgggg                                                                                            >  1:710668/1‑96 (MQ=255)
                                                                                                              tgtgCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCgggg                                                                                            <  2:710668/96‑1 (MQ=255)
                                                                                                                 gCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTACGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACAGCGTCGTTGAAATTCGGCGACTGGATTATGAATTCTTCCGtt                                   >  2:755993/1‑150 (MQ=255)
                                                                                                                  ccGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTg                                  >  2:1323672/1‑150 (MQ=255)
                                                                                                                    ggTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGcc                                >  2:62925/1‑150 (MQ=255)
                                                                                                                      tGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCAc                              >  2:5093/1‑150 (MQ=255)
                                                                                                                       ggCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACg                             <  1:1576855/150‑1 (MQ=255)
                                                                                                                        gCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGt                            >  1:1700235/1‑150 (MQ=255)
                                                                                                                         ggagaAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGtt                           <  2:1068557/149‑1 (MQ=255)
                                                                                                                         cGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGtt                           >  1:893055/1‑150 (MQ=255)
                                                                                                                           agaAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTtgg                         <  2:210878/150‑1 (MQ=255)
                                                                                                                               aaCCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTtggtgg                      >  2:1439120/1‑149 (MQ=255)
                                                                                                                               aaCCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTtggtgg                      <  1:1439120/149‑1 (MQ=255)
                                                                                                                                  cGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGAc                  >  2:1132774/1‑150 (MQ=255)
                                                                                                                                        gcaCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGCCCGCGTCGTTGAAATGCGGCGACTGGATTAGGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAAt            >  2:120516/3‑150 (MQ=255)
                                                                                                                                         caCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATg           >  1:507357/2‑150 (MQ=255)
                                                                                                                                         aaCAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATg           >  1:590616/1‑150 (MQ=255)
                                                                                                                                          aCAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGt          >  1:632592/1‑150 (MQ=255)
                                                                                                                                          aCAGGTGGCAAACGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGt          >  2:401195/1‑150 (MQ=255)
                                                                                                                                             ggTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTcc       <  1:1313039/150‑1 (MQ=255)
                                                                                                                                                  cGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATAcc  <  1:1564189/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTCTCCCTTTGTGGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAAGAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATATCGACCGCGTCGTTGAAATGCGGCGACTGGATTATGAAGTCTGCCGTTGCCACGTTGGTGGCGACCGGAATGTTCCATACC  >  NC_000913/1026391‑1026686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: