Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 65,813 A→C 6.3% intergenic (‑33/+42) polB ← / ← araD DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091365,8130AC 6.3% 340.9 / 10.8 122intergenic (‑33/+42)polB/araDDNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (60/53);  new base C (5/3);  total (66/56)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

Only 100 of 124 total aligned reads displayed.

TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATA  >  NC_000913/65664‑65958
                                                                                                                                                     |                                                                                                                                                 
 tGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGtat                                                                                                                                                  <  1:497280/150‑1 (MQ=255)
    ggTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGc                                                                                                                                               <  1:323386/150‑1 (MQ=255)
       gCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTg                                                                                                                                            <  2:1678356/150‑1 (MQ=255)
       gCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTg                                                                                                                                            >  2:1451228/1‑150 (MQ=255)
         aaGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGt                                                                                                                                          >  1:124289/1‑150 (MQ=255)
          aGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTATACTCAAGCCTGGtt                                                                                                                                         <  2:1123172/150‑1 (MQ=255)
           gCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTATACTCAAGCCTGGttt                                                                                                                                        <  1:296949/150‑1 (MQ=255)
            cGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGCCTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTc                                                                                                                                       >  1:1221853/1‑150 (MQ=255)
              tAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTATACTCAAGCCTGGtttttt                                                                                                                                     <  1:743835/150‑4 (MQ=255)
               aaCCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGtt                                                                                                                                    <  2:1568794/150‑1 (MQ=255)
                 ccTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGGGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTg                                                                                                                                  <  2:1156033/150‑1 (MQ=255)
                  cTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGa                                                                                                                                 >  1:166524/1‑150 (MQ=255)
                   tGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGGTTCGTATGAt                                                                                                                                >  1:1073208/1‑150 (MQ=255)
                      aaCGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTgg                                                                                                                             >  1:1567276/1‑150 (MQ=255)
                          gCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCtgt                                                                                                                         >  1:1019274/1‑150 (MQ=255)
                           cccGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCtgtg                                                                                                                        >  1:559046/1‑150 (MQ=255)
                           cccGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCtgtg                                                                                                                        <  2:1304359/150‑1 (MQ=255)
                                tGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGtttt                                                                                                                   <  2:1567213/150‑1 (MQ=255)
                                tGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGtttt                                                                                                                   >  2:135297/1‑150 (MQ=255)
                                 gtcggtcgCCAGACAGAAGGAGACGTCGGTCCCTTGCTGGGTGTCCCGCAAGTGGCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCTTCAAAAAACCAGGCTCGAGTATTGCCTGGTTTCGTTTGATTGGCTGTGGTTTta                                                                                                                  <  1:1426117/150‑1 (MQ=255)
                                 gTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGACCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTta                                                                                                                  >  1:292635/1‑150 (MQ=255)
                                    cGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                                                                               <  2:976573/150‑1 (MQ=255)
                                             ccagAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTAc                                                                                                      <  2:1637184/150‑1 (MQ=255)
                                              cagAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTAcg                                                                                                     >  1:1496745/1‑149 (MQ=255)
                                                  aGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGccc                                                                                                 <  2:1647887/150‑1 (MQ=255)
                                                     agaCTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTa                                                                                              <  1:1442217/150‑1 (MQ=255)
                                                       aCTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAat                                                                                            >  1:1233563/1‑150 (MQ=255)
                                                        cTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCTTAata                                                                                           >  1:1387019/1‑150 (MQ=255)
                                                          tCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATg                                                                                         >  1:60351/1‑150 (MQ=255)
                                                          tCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACGGGCAGTAatgac                                                                                         <  1:477830/150‑4 (MQ=255)
                                                          tCTGTCCCTTGCGGGGTGTCACGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTTGTTTTATACAGTCATTACTGCCCGTAATATg                                                                                         <  1:126362/150‑1 (MQ=255)
                                                             gTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCt                                                                                      <  1:1451228/150‑1 (MQ=255)
                                                               cccTTGCGGAGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTc                                                                                    >  1:486965/1‑150 (MQ=255)
                                                                 cTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTTGCTGTGGTTTTATACAGTCAATACTGCCCGTAAAATGCCATcgc                                                                                  >  2:787351/1‑150 (MQ=255)
                                                                 cTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTcgc                                                                                  >  2:1650003/1‑150 (MQ=255)
                                                                    gCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATGAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGcc                                                                               <  1:233530/150‑1 (MQ=255)
                                                                    gCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGcc                                                                               <  2:626529/150‑1 (MQ=255)
                                                                    gCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGcc                                                                               >  1:1060793/1‑150 (MQ=255)
                                                                     cGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCa                                                                              <  2:1519313/150‑1 (MQ=255)
                                                                      ggggTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCAt                                                                             <  2:1060793/150‑1 (MQ=255)
                                                                             cccGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCTCGCTGAAAATCCATCAAAAAACCAGGCTTGAGGATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACg                                                                      >  1:862720/1‑150 (MQ=255)
                                                                             cccGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACg                                                                      <  1:698029/150‑1 (MQ=255)
                                                                                  cAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTGGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGAt                                                                 >  1:1137534/1‑150 (MQ=255)
                                                                                  cAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGAt                                                                 <  1:580491/150‑1 (MQ=255)
                                                                                  cAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTATCGTTAGATTGGCTCTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCTTGCTTACGGAgct                                                                 >  1:890243/1‑148 (MQ=255)
                                                                                    gtgtCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGCAATATGCCTTCGCGCCATGCTTACGCAGATAg                                                               <  2:536713/150‑1 (MQ=255)
                                                                                    gtgtCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGCAATATGCCTTCGCGCCATGCTTACGCAGATAg                                                               <  2:124289/150‑1 (MQ=255)
                                                                                     tgtCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAgt                                                              >  2:407051/1‑150 (MQ=255)
                                                                                       tctgtttATGATATAACCTGACTGCGACAAGCTGAAAATCCATAAATAAACGTGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATTCAGTCATTACTGCCCGTTATATGGCTTCGCGCCATGCTTACGCAGATAgtgt                                                            <  1:787351/145‑1 (MQ=255)
                                                                                         gggTTAAGATACATCCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCATGCTAGAGTATAGACTGGTTTCCTTTGATTGGCTGTGATTTTATACAGTCATTACTGGCCGGCTTATGCGTTAGAGCCATGCTTCCGCAGATTGTGttt                                                          >  1:150661/1‑150 (MQ=255)
                                                                                         gggTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGttt                                                          >  1:1189817/1‑150 (MQ=255)
                                                                                           gTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTAt                                                        <  2:1613134/150‑1 (MQ=255)
                                                                                             tAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATcc                                                      <  2:1564416/150‑1 (MQ=255)
                                                                                               aGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCcag                                                    >  1:626662/1‑150 (MQ=255)
                                                                                               aGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCcag                                                    >  2:1329965/1‑150 (MQ=255)
                                                                                                gATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTTTAGCCTGGTTTCGTTTGATTGGCTGTGTTTTTATACAGTCATGACTGCCCGTAATATGCCTTCGCGCCATGCTTTCGCAGATTGTGTTTATCctgc                                                   >  2:315380/1‑150 (MQ=255)
                                                                                                gATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTCCGCAGATAGTGTTTATCcagc                                                   >  2:166542/1‑150 (MQ=255)
                                                                                                gATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCcagc                                                   >  1:782428/1‑150 (MQ=255)
                                                                                                 aTAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTTAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGAGCCATGCTTACGCAGATACTGTTTATCcagca                                                  >  1:138926/1‑150 (MQ=255)
                                                                                                 aTAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTAATTCACGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCcagca                                                  >  2:32526/1‑150 (MQ=255)
                                                                                                  tAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCcagcag                                                 >  1:1412185/1‑150 (MQ=255)
                                                                                                  tAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCcagcag                                                 <  2:60351/150‑1 (MQ=255)
                                                                                                  tAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCcagcag                                                 <  2:559046/150‑1 (MQ=255)
                                                                                                        ctgcctgcGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTtg                                           <  2:292635/150‑1 (MQ=255)
                                                                                                          gcctgcGCCACGCTGAAAATCCATCAAAAAACGAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTtgct                                         <  1:1616047/150‑1 (MQ=255)
                                                                                                          gcctgcGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTtgct                                         >  2:1574139/1‑150 (MQ=255)
                                                                                                          gcctgcGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTtgct                                         >  2:1716127/1‑150 (MQ=255)
                                                                                                          gcctgcGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCATCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTtgct                                         >  2:1078831/1‑150 (MQ=255)
                                                                                                           cctgcGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTtgctg                                        >  1:1163570/1‑150 (MQ=255)
                                                                                                           cctgcGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTtgctg                                        <  2:581808/150‑1 (MQ=255)
                                                                                                            ctgcGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTtgctgc                                       <  1:1375362/150‑1 (MQ=255)
                                                                                                             tgcGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCa                                      <  2:326343/150‑1 (MQ=255)
                                                                                                              gcgcCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCat                                     <  2:486965/150‑1 (MQ=255)
                                                                                                                  cACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATcc                                 <  2:563104/150‑1 (MQ=255)
                                                                                                                  cACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATcc                                 <  1:890244/150‑1 (MQ=255)
                                                                                                                   aCGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCg                                >  2:666736/1‑150 (MQ=255)
                                                                                                                      cTGAAAATCCATCAAAATAACAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTTCTGCATATCCGGTa                             <  1:169612/150‑1 (MQ=255)
                                                                                                                       tGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTaa                            <  2:1153715/150‑1 (MQ=255)
                                                                                                                       tGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTaa                            <  2:1406020/150‑1 (MQ=255)
                                                                                                                        gAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAAc                           >  1:1628950/1‑150 (MQ=255)
                                                                                                                        gAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTCGTTTCGTTTGATTGGCAGTGGATTTATACAGTCAATACTGCCCGTAATATGCCATCGAGCCAAGCTTACGCAGATAGTGTTAATCCAGCAGCGTTTGCTGCATATCCGGTAAc                           >  2:1337066/1‑150 (MQ=255)
                                                                                                                        gAAAATCCACCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAAc                           >  2:386443/1‑150 (MQ=255)
                                                                                                                           aaTCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTgc                        >  2:907324/1‑150 (MQ=255)
                                                                                                                           aaTCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTAATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTgc                        >  2:1637160/1‑150 (MQ=255)
                                                                                                                               aaTCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTgcggcg                    <  2:1672229/149‑1 (MQ=255)
                                                                                                                                  cAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTa                 >  2:403195/1‑150 (MQ=255)
                                                                                                                                  cAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTa                 >  2:245701/1‑150 (MQ=255)
                                                                                                                                  aacgaaaCCAGGCTATACTCAAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTa                 >  1:1630686/5‑150 (MQ=255)
                                                                                                                                    cgaaaCCAGGCTATACTCAAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAAc               >  1:1442959/3‑150 (MQ=255)
                                                                                                                                      aaaCCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTg             >  2:964233/1‑150 (MQ=255)
                                                                                                                                      aaaCCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTg             >  2:529599/1‑150 (MQ=255)
                                                                                                                                      aaaCCAGGCTATACTCAAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTGACTg             >  1:1163059/1‑150 (MQ=255)
                                                                                                                                      aaaCCAGGCTATACTCAAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTg             >  1:97567/1‑150 (MQ=255)
                                                                                                                                         ccAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACg          <  2:1378099/150‑1 (MQ=255)
                                                                                                                                         ccAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATACGGTAACTGCGGCGCTAACTGACg          >  1:1170735/1‑150 (MQ=255)
                                                                                                                                           aGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGc        <  2:1412185/150‑1 (MQ=255)
                                                                                                                                            ggCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCa       >  1:1075960/1‑150 (MQ=255)
                                                                                                                                               ttGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGaa    >  1:416580/1‑150 (MQ=255)
                                                                                                                                                tGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCATTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAat   >  1:1395576/1‑150 (MQ=255)
                                                                                                                                                 gAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAata  <  2:1596756/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATA  >  NC_000913/65664‑65958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: