Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,052,260 T→C 5.6% intergenic (+20/+29) gnsA → / ← yccM putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,052,2600TC 5.6% 262.9 / 5.1 90intergenic (+20/+29)gnsA/yccMputative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein
Reads supporting (aligned to +/- strand):  ref base T (39/45);  new base C (3/2);  total (43/47)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-01

CGACTTTATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACC  >  NC_000913/1052111‑1052409
                                                                                                                                                     |                                                                                                                                                     
cGACTTTATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGt                                                                                                                                                       >  2:1545346/1‑150 (MQ=255)
 gACTTTATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTg                                                                                                                                                      <  2:458789/150‑1 (MQ=255)
 gACTTTATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTg                                                                                                                                                      <  1:1252339/150‑1 (MQ=255)
   cTTTATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAAAGACCGGGCTGGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCg                                                                                                                                                    <  2:851342/150‑1 (MQ=255)
    tttATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGGTGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGa                                                                                                                                                   <  1:1338810/150‑1 (MQ=255)
     ttttCGCGCTAAAAATCGCCGAGCTGAACAAGAATACAGGGATAGAAGTCTCTGAAATTCGCTTCACCGCTCGAGTAAAAATGACCGGCCTTGAAAGTTATGATGTCAAAATCAAACTAATGTGATTTAGTGAACATCACCCCGTGCGAg                                                                                                                                                  <  1:1675532/147‑1 (MQ=255)
     ttATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAg                                                                                                                                                  <  1:911121/150‑1 (MQ=255)
            gcAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGt                                                                                                                                           <  1:1645432/150‑1 (MQ=255)
             cAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGtt                                                                                                                                          >  1:946793/1‑150 (MQ=255)
              aaaaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATGTTc                                                                                                                                         <  2:1169504/150‑1 (MQ=255)
              aaaaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTc                                                                                                                                         <  1:827617/150‑1 (MQ=255)
                aaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATGTTcac                                                                                                                                       <  1:876238/150‑3 (MQ=255)
                aaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGGCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCg                                                                                                                                       <  1:144210/150‑1 (MQ=255)
                 aaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGc                                                                                                                                      <  1:364437/150‑1 (MQ=255)
                 aaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAACTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATCATGTCAAAATCAAAATAATGAGATATTGTGAACATCACCCCGTGCGAGGTGATGTTCAGc                                                                                                                                      >  1:351975/1‑150 (MQ=255)
                   aTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCtt                                                                                                                                    >  2:1426248/1‑150 (MQ=255)
                       ccGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCttgttg                                                                                                                                >  1:1455469/1‑150 (MQ=255)
                         taGCTGAACAAGAATACAGGGAAAGAAGTCTCTGTAATTCGCTTCACCGCACGAGAAATAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCt                                                                                                                              <  1:402684/149‑1 (MQ=255)
                         gAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCt                                                                                                                              >  2:943162/1‑150 (MQ=255)
                           gCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTaa                                                                                                                            >  2:1467353/1‑150 (MQ=255)
                              gAACAACAATACAGGGAAAGAAGTCTATTAAATTCTCTTCACCGCACGAGAAAAAATGACCGGTCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCTAGGTTATGTTCCGCTTGTTGCTAAttt                                                                                                                         <  2:833037/150‑1 (MQ=255)
                                aCAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAATGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAg                                                                                                                       <  1:21047/150‑1 (MQ=255)
                                aCAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCTAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGATCAGCTTGTTGCTAATTTat                                                                                                                       >  1:503806/1‑149 (MQ=255)
                                     aaTACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGAcc                                                                                                                  <  2:946793/150‑1 (MQ=255)
                                        aCAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAAt                                                                                                               <  2:968614/150‑1 (MQ=255)
                                         cAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATc                                                                                                              >  2:1555304/1‑150 (MQ=255)
                                                  aagTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCt                                                                                                     <  2:1612076/150‑1 (MQ=255)
                                                   agTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCtt                                                                                                    >  2:142197/1‑150 (MQ=255)
                                                   agTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCtt                                                                                                    >  2:380923/1‑150 (MQ=255)
                                                    gTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtg                                                                                                   >  2:1503942/1‑150 (MQ=255)
                                                     tctcTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgt                                                                                                  <  1:792864/150‑1 (MQ=255)
                                                      ctctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgtg                                                                                                 <  1:1408677/150‑1 (MQ=255)
                                                      ctctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTCCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgtg                                                                                                 <  2:7416/150‑1 (MQ=255)
                                                       tctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTgg                                                                                                >  1:1049622/1‑150 (MQ=255)
                                                        ctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGa                                                                                               >  1:892845/1‑150 (MQ=255)
                                                                cGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCg                                                                                       >  1:1614080/1‑150 (MQ=255)
                                                                 gCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGt                                                                                      <  1:1057980/150‑1 (MQ=255)
                                                                    tCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCgg                                                                                   <  1:755392/150‑1 (MQ=255)
                                                                     cACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGt                                                                                  <  2:778142/150‑1 (MQ=255)
                                                                     cACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGt                                                                                  <  2:1614080/150‑1 (MQ=255)
                                                                       ccGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTAc                                                                                >  1:1627450/1‑150 (MQ=255)
                                                                        cGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACa                                                                               >  2:1342678/1‑150 (MQ=255)
                                                                          cACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAAt                                                                             >  2:235998/1‑150 (MQ=255)
                                                                            cGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtc                                                                           <  1:1545346/150‑1 (MQ=255)
                                                                             gagaAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACAAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcc                                                                          >  1:80378/1‑150 (MQ=255)
                                                                               gaAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTACTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcctc                                                                        >  1:1450714/1‑150 (MQ=255)
                                                                                 aaaaaTGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCg                                                                      >  1:1643486/1‑150 (MQ=255)
                                                                                        aCCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCgg                                                               >  2:1580179/1‑150 (MQ=255)
                                                                                         ccGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCggg                                                              >  2:215224/1‑150 (MQ=255)
                                                                                            ggCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCtt                                                           >  1:1099460/1‑150 (MQ=255)
                                                                                              cTTGAAAGTTATGATGGCAAAATCAAAATAATGTGATTTTGTGTACATCACCCAGTGCGAGGTGTTGTTCCGCTTGTTGCTAATTTAGTGTCCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTg                                                         <  2:351975/150‑1 (MQ=255)
                                                                                                tGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGcc                                                       >  1:758798/1‑150 (MQ=255)
                                                                                                tGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTACGCTTGTTGCTAATATAGTGCCCAATCATTGGCGCTTGTGGAATTAATCTTCGGTACAATACCTCCGGCACCGGGCTTTGcc                                                       >  2:825188/1‑150 (MQ=255)
                                                                                                  aaaGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCAt                                                     >  2:1486393/1‑150 (MQ=255)
                                                                                                  aaaGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCAt                                                     <  1:1034634/150‑1 (MQ=255)
                                                                                                    aGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGTTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATAc                                                   <  2:209835/150‑1 (MQ=255)
                                                                                                         tgatgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCg                                              <  2:1304976/150‑1 (MQ=255)
                                                                                                         tgatgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGAACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCg                                              >  1:754356/1‑150 (MQ=255)
                                                                                                         tgatgTCAAAATCAAAATAAAGTGATTTTGTGAACATCACCCCGAGCGAGGTGATGTTCCGCTTGTTGATAATTTAGAGACAAATCATTGTCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCTGAACCGTTCATTGACATACTCACg                                              >  2:1133719/1‑150 (MQ=255)
                                                                                                            tgTCAAAATCAAAATTATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCAt                                           <  1:800821/150‑1 (MQ=255)
                                                                                                            tgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCAt                                           >  1:847180/1‑150 (MQ=255)
                                                                                                             gTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATa                                          >  1:572150/1‑150 (MQ=255)
                                                                                                              tcaaaatcaaaaTAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATAc                                         <  1:1555304/150‑1 (MQ=255)
                                                                                                               caaaatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACa                                        >  1:398691/1‑150 (MQ=255)
                                                                                                                 aaatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGACACCGGGCTTTGCCATACTCCCGCATACAtt                                      >  1:1478640/1‑150 (MQ=255)
                                                                                                                  aatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                     <  1:380923/150‑1 (MQ=255)
                                                                                                                  aatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                     <  1:1503942/150‑1 (MQ=255)
                                                                                                                   atcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGc                                    <  1:235998/150‑1 (MQ=255)
                                                                                                                   atcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGc                                    <  1:215224/150‑1 (MQ=255)
                                                                                                                     caaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGGTAATTTAGTGACCAATCCTTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGt                                  <  2:114446/150‑1 (MQ=255)
                                                                                                                     caaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGt                                  <  1:1288649/150‑1 (MQ=255)
                                                                                                                      aaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTa                                 <  2:1058540/150‑1 (MQ=255)
                                                                                                                          taatGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCa                             >  2:1539637/1‑150 (MQ=255)
                                                                                                                             tgtgATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACAAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATc                          <  2:80378/150‑1 (MQ=255)
                                                                                                                                gATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCAcc                       >  2:1208511/1‑150 (MQ=255)
                                                                                                                                gATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCAcc                       >  1:595591/1‑150 (MQ=255)
                                                                                                                                gATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCAcc                       >  1:1206566/1‑150 (MQ=255)
                                                                                                                                gATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACAAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCAcc                       >  1:286717/1‑150 (MQ=255)
                                                                                                                                 aTTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCg                      <  1:943162/150‑1 (MQ=255)
                                                                                                                                  ttttGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGc                     <  2:892845/150‑1 (MQ=255)
                                                                                                                                      gtgAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTTACCAATCACCGCAAAc                 >  2:1146124/1‑150 (MQ=255)
                                                                                                                                      cggAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAAc                 >  1:366206/3‑150 (MQ=255)
                                                                                                                                      cggAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAAACAATCACAGCAAAc                 >  2:1542511/3‑150 (MQ=255)
                                                                                                                                        gAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACAt               >  2:1391070/1‑150 (MQ=255)
                                                                                                                                         aaCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTTGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATa              <  1:142197/150‑1 (MQ=255)
                                                                                                                                         aaCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCCCCGCACACATa              >  2:469633/1‑150 (MQ=255)
                                                                                                                                         aaCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATa              <  1:1208511/150‑1 (MQ=255)
                                                                                                                                         aaCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATa              >  2:1230010/1‑150 (MQ=255)
                                                                                                                                              cACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATccc         <  2:1455469/150‑1 (MQ=255)
                                                                                                                                                ccccGTGCGAGGTGATGGTCCGCTTGGTGCTAATTTAGTGACCTATCATTGGCGCGTGTGGTATTAAGCGTCGGTACAATTCCTCCGGCCCCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCCCCGCAAACATAATCCCCa       <  2:1643486/150‑1 (MQ=255)
                                                                                                                                                  ccGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGACACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGa     <  2:1478640/150‑1 (MQ=255)
                                                                                                                                                     tGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAAcc  <  2:1206566/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGACTTTATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACC  >  NC_000913/1052111‑1052409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑