Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,052,264 A→G 5.7% intergenic (+24/+25) gnsA → / ← yccM putative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,052,2640AG 5.7% 262.5 / 5.0 88intergenic (+24/+25)gnsA/yccMputative phosphatidylethanolamine synthesis regulator/putative 4Fe‑4S membrane protein
Reads supporting (aligned to +/- strand):  ref base A (41/42);  new base G (3/2);  total (44/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

TTTATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTA  >  NC_000913/1052115‑1052413
                                                                                                                                                     |                                                                                                                                                     
tttATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGGTGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGa                                                                                                                                                       <  1:1338810/150‑1 (MQ=255)
 ttttCGCGCTAAAAATCGCCGAGCTGAACAAGAATACAGGGATAGAAGTCTCTGAAATTCGCTTCACCGCTCGAGTAAAAATGACCGGCCTTGAAAGTTATGATGTCAAAATCAAACTAATGTGATTTAGTGAACATCACCCCGTGCGAg                                                                                                                                                      <  1:1675532/147‑1 (MQ=255)
 ttATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAg                                                                                                                                                      <  1:911121/150‑1 (MQ=255)
        gcAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGt                                                                                                                                               <  1:1645432/150‑1 (MQ=255)
         cAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGtt                                                                                                                                              >  1:946793/1‑150 (MQ=255)
          aaaaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATGTTc                                                                                                                                             <  2:1169504/150‑1 (MQ=255)
          aaaaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTc                                                                                                                                             <  1:827617/150‑1 (MQ=255)
            aaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCTCGCACGGGGTGATGTTcac                                                                                                                                           <  1:876238/150‑3 (MQ=255)
            aaaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGGCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCg                                                                                                                                           <  1:144210/150‑1 (MQ=255)
             aaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGc                                                                                                                                          <  1:364437/150‑1 (MQ=255)
             aaaTCGCCGAGCTGAACAAGAATACAGGGAAAGAACTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATCATGTCAAAATCAAAATAATGAGATATTGTGAACATCACCCCGTGCGAGGTGATGTTCAGc                                                                                                                                          >  1:351975/1‑150 (MQ=255)
               aTCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCtt                                                                                                                                        >  2:1426248/1‑150 (MQ=255)
                   ccGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCttgttg                                                                                                                                    >  1:1455469/1‑150 (MQ=255)
                     taGCTGAACAAGAATACAGGGAAAGAAGTCTCTGTAATTCGCTTCACCGCACGAGAAATAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCt                                                                                                                                  <  1:402684/149‑1 (MQ=255)
                     gAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCt                                                                                                                                  >  2:943162/1‑150 (MQ=255)
                       gCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTaa                                                                                                                                >  2:1467353/1‑150 (MQ=255)
                          gAACAACAATACAGGGAAAGAAGTCTATTAAATTCTCTTCACCGCACGAGAAAAAATGACCGGTCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCTAGGTTATGTTCCGCTTGTTGCTAAttt                                                                                                                             <  2:833037/150‑1 (MQ=255)
                            aCAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAATGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAg                                                                                                                           <  1:21047/150‑1 (MQ=255)
                            aCAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCTAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGATCAGCTTGTTGCTAATTTat                                                                                                                           >  1:503806/1‑149 (MQ=255)
                                 aaTACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGAcc                                                                                                                      <  2:946793/150‑1 (MQ=255)
                                    aCAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAAt                                                                                                                   <  2:968614/150‑1 (MQ=255)
                                     cAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATc                                                                                                                  >  2:1555304/1‑150 (MQ=255)
                                              aagTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCt                                                                                                         <  2:1612076/150‑1 (MQ=255)
                                               agTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCtt                                                                                                        >  2:142197/1‑150 (MQ=255)
                                               agTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCtt                                                                                                        >  2:380923/1‑150 (MQ=255)
                                                gTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtg                                                                                                       >  2:1503942/1‑150 (MQ=255)
                                                 tctcTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgt                                                                                                      <  1:792864/150‑1 (MQ=255)
                                                  ctctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgtg                                                                                                     <  1:1408677/150‑1 (MQ=255)
                                                  ctctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTCCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTtgtg                                                                                                     <  2:7416/150‑1 (MQ=255)
                                                   tctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTgg                                                                                                    >  1:1049622/1‑150 (MQ=255)
                                                    ctGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGa                                                                                                   >  1:892845/1‑150 (MQ=255)
                                                            cGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCg                                                                                           >  1:1614080/1‑150 (MQ=255)
                                                             gCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGt                                                                                          <  1:1057980/150‑1 (MQ=255)
                                                                tCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCgg                                                                                       <  1:755392/150‑1 (MQ=255)
                                                                 cACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGt                                                                                      <  2:778142/150‑1 (MQ=255)
                                                                 cACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGt                                                                                      <  2:1614080/150‑1 (MQ=255)
                                                                   ccGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTAc                                                                                    >  1:1627450/1‑150 (MQ=255)
                                                                    cGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACa                                                                                   >  2:1342678/1‑150 (MQ=255)
                                                                      cACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAAt                                                                                 >  2:235998/1‑150 (MQ=255)
                                                                        cGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtc                                                                               <  1:1545346/150‑1 (MQ=255)
                                                                         gagaAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACAAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcc                                                                              >  1:80378/1‑150 (MQ=255)
                                                                           gaAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTACTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATtcctc                                                                            >  1:1450714/1‑150 (MQ=255)
                                                                             aaaaaTGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCg                                                                          >  1:1643486/1‑150 (MQ=255)
                                                                                    aCCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCgg                                                                   >  2:1580179/1‑150 (MQ=255)
                                                                                     ccGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCggg                                                                  >  2:215224/1‑150 (MQ=255)
                                                                                        ggCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCtt                                                               >  1:1099460/1‑150 (MQ=255)
                                                                                          cTTGAAAGTTATGATGGCAAAATCAAAATAATGTGATTTTGTGTACATCACCCAGTGCGAGGTGTTGTTCCGCTTGTTGCTAATTTAGTGTCCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTg                                                             <  2:351975/150‑1 (MQ=255)
                                                                                            tGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGcc                                                           >  1:758798/1‑150 (MQ=255)
                                                                                            tGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTACGCTTGTTGCTAATATAGTGCCCAATCATTGGCGCTTGTGGAATTAATCTTCGGTACAATACCTCCGGCACCGGGCTTTGcc                                                           >  2:825188/1‑150 (MQ=255)
                                                                                              aaaGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCAt                                                         >  2:1486393/1‑150 (MQ=255)
                                                                                              aaaGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCAt                                                         <  1:1034634/150‑1 (MQ=255)
                                                                                                aGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGTTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATAc                                                       <  2:209835/150‑1 (MQ=255)
                                                                                                     tgatgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCg                                                  <  2:1304976/150‑1 (MQ=255)
                                                                                                     tgatgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGAACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCg                                                  >  1:754356/1‑150 (MQ=255)
                                                                                                     tgatgTCAAAATCAAAATAAAGTGATTTTGTGAACATCACCCCGAGCGAGGTGATGTTCCGCTTGTTGATAATTTAGAGACAAATCATTGTCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCTGAACCGTTCATTGACATACTCACg                                                  >  2:1133719/1‑150 (MQ=255)
                                                                                                        tgTCAAAATCAAAATTATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCAt                                               <  1:800821/150‑1 (MQ=255)
                                                                                                        tgTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCAt                                               >  1:847180/1‑150 (MQ=255)
                                                                                                         gTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATa                                              >  1:572150/1‑150 (MQ=255)
                                                                                                          tcaaaatcaaaaTAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATAc                                             <  1:1555304/150‑1 (MQ=255)
                                                                                                           caaaatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACa                                            >  1:398691/1‑150 (MQ=255)
                                                                                                             aaatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGACACCGGGCTTTGCCATACTCCCGCATACAtt                                          >  1:1478640/1‑150 (MQ=255)
                                                                                                              aatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                         <  1:380923/150‑1 (MQ=255)
                                                                                                              aatcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTg                                         <  1:1503942/150‑1 (MQ=255)
                                                                                                               atcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGc                                        <  1:215224/150‑1 (MQ=255)
                                                                                                               atcaaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGc                                        <  1:235998/150‑1 (MQ=255)
                                                                                                                 caaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGGTAATTTAGTGACCAATCCTTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGt                                      <  2:114446/150‑1 (MQ=255)
                                                                                                                 caaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGt                                      <  1:1288649/150‑1 (MQ=255)
                                                                                                                  aaaatAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTa                                     <  2:1058540/150‑1 (MQ=255)
                                                                                                                      taatGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCa                                 >  2:1539637/1‑150 (MQ=255)
                                                                                                                         tgtgATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACAAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATc                              <  2:80378/150‑1 (MQ=255)
                                                                                                                            gATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCAcc                           >  1:1206566/1‑150 (MQ=255)
                                                                                                                            gATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCAcc                           >  2:1208511/1‑150 (MQ=255)
                                                                                                                            gATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCAcc                           >  1:595591/1‑150 (MQ=255)
                                                                                                                            gATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACAAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCAcc                           >  1:286717/1‑150 (MQ=255)
                                                                                                                             aTTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCg                          <  1:943162/150‑1 (MQ=255)
                                                                                                                              ttttGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGc                         <  2:892845/150‑1 (MQ=255)
                                                                                                                                  gtgAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTTACCAATCACCGCAAAc                     >  2:1146124/1‑150 (MQ=255)
                                                                                                                                  cggAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAAc                     >  1:366206/3‑150 (MQ=255)
                                                                                                                                  cggAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAAACAATCACAGCAAAc                     >  2:1542511/3‑150 (MQ=255)
                                                                                                                                    gAACATCACCTCGCACGGGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACAt                   >  2:1391070/1‑150 (MQ=255)
                                                                                                                                     aaCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTTGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATa                  <  1:142197/150‑1 (MQ=255)
                                                                                                                                     aaCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCCCCGCACACATa                  >  2:469633/1‑150 (MQ=255)
                                                                                                                                     aaCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATa                  >  2:1230010/1‑150 (MQ=255)
                                                                                                                                     aaCATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATa                  <  1:1208511/150‑1 (MQ=255)
                                                                                                                                          cACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATccc             <  2:1455469/150‑1 (MQ=255)
                                                                                                                                            ccccGTGCGAGGTGATGGTCCGCTTGGTGCTAATTTAGTGACCTATCATTGGCGCGTGTGGTATTAAGCGTCGGTACAATTCCTCCGGCCCCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCCCCGCAAACATAATCCCCa           <  2:1643486/150‑1 (MQ=255)
                                                                                                                                              ccGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGACACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGa         <  2:1478640/150‑1 (MQ=255)
                                                                                                                                                 tGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAAcc      <  2:1206566/150‑1 (MQ=255)
                                                                                                                                                   cGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAg    >  1:1374757/1‑150 (MQ=255)
                                                                                                                                                     aGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTa  >  1:516350/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTATCGCGCAAAAAATCGCCGAGCTGAACAAGAATACAGGGAAAGAAGTCTCTGAAATTCGCTTCACCGCACGAGAAAAAATGACCGGGCTTGAAAGTTATGATGTCAAAATCAAAATAATGTGATTTTGTGAACATCACCCCGTGCGAGGTGATGTTCCGCTTGTTGCTAATTTAGTGACCAATCATTGGCGCTTGTGGAATTAAGCGTCGGTACAATTCCTCCGGCACCGGGCTTTGCCATACTCCCGCATACATTGCGTAACCAATCACCGCAAACATAATCCCCAGAACCAGTA  >  NC_000913/1052115‑1052413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: