BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,064,161	G→A	12.7%	L42L (CTG→CTA) ‡	yccE →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,064,161	0	G	A	12.7%	278.5 / 21.2	102	L42L (CTG→CTA) ‡	yccE	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (49/40); new base A (4/9); total (53/49)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 105 total aligned reads displayed.

TATTAAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAA  >  NC_000913/1064012‑1064310

tattaAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTg                                                                                                                                                       <  2:834279/150‑1 (MQ=255)

 attaAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTgg                                                                                                                                                      >  2:616554/1‑150 (MQ=255)

   taAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtg                                                                                                                                                    <  2:149834/150‑1 (MQ=255)

      aTTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGgtgtgt                                                                                                                                                 <  1:1711586/150‑1 (MQ=255)

          tataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTa                                                                                                                                             <  1:372567/150‑1 (MQ=255)

          tataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttc                                                                                                                                             <  1:270010/150‑2 (MQ=255)

          tataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttc                                                                                                                                             <  2:66359/150‑2 (MQ=255)

          tataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttc                                                                                                                                             <  2:1672167/150‑2 (MQ=255)

           ataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttca                                                                                                                                            <  2:90756/150‑3 (MQ=255)

           ataGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttca                                                                                                                                            <  2:120621/150‑3 (MQ=255)

            taGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttcaa                                                                                                                                           <  2:1104099/150‑4 (MQ=255)

            taGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttcaa                                                                                                                                           <  2:1549408/150‑4 (MQ=255)

             aGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGc                                                                                                                                          <  1:1169166/150‑1 (MQ=255)

              gACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCg                                                                                                                                         >  2:666874/1‑150 (MQ=255)

                cGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGt                                                                                                                                       <  2:947884/150‑1 (MQ=255)

                cGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACCAGTGTGttttcaaaaag                                                                                                                                       <  1:1297873/150‑8 (MQ=255)

                  cTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTta                                                                                                                                     >  2:1717016/1‑150 (MQ=255)

                  cTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTta                                                                                                                                     >  1:1328549/1‑150 (MQ=255)

                    atatatGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTtata                                                                                                                                   <  1:1610602/150‑1 (MQ=255)

                       tatGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGTTTATACaa                                                                                                                                >  1:1472508/1‑150 (MQ=255)

                        atGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAAt                                                                                                                               >  1:479817/1‑150 (MQ=255)

                        atGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAAt                                                                                                                               <  2:505684/150‑1 (MQ=255)

                          gggTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGa                                                                                                                             >  1:165637/1‑150 (MQ=255)

                                taATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAg                                                                                                                       <  2:575496/150‑1 (MQ=255)

                                 aaTATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTTGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGa                                                                                                                      <  1:850071/150‑1 (MQ=255)

                                   tataCATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACg                                                                                                                    <  2:1225461/150‑1 (MQ=255)

                                       cATGGAATTAGTTGCACTGCAAATAAATATATGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTAATACAATGAACTCAGACgtaaa                                                                                                                >  1:1645443/1‑150 (MQ=255)

                                        aTGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaag                                                                                                               <  1:1203215/150‑1 (MQ=255)

                                         tGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagt                                                                                                              >  1:1594959/1‑150 (MQ=255)

                                         tGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACgtaaagt                                                                                                              <  1:1440410/150‑1 (MQ=255)

                                             attattTGCAATTCAAATAATTATTTGAAACATGCCTTGAAAGAAATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAAt                                                                                                          <  1:321523/150‑1 (MQ=255)

                                               tAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGa                                                                                                        <  2:1695552/150‑1 (MQ=255)

                                                aGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCATACGTAAATTAAATGaa                                                                                                       >  1:58011/1‑150 (MQ=255)

                                                 gTTGCCCTGCGAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAAGCAACCATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCATACGTAAAGTAAATGAag                                                                                                      >  2:432004/1‑150 (MQ=255)

                                                    gCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAgg                                                                                                   <  2:1482464/150‑1 (MQ=255)

                                                        tGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATAc                                                                                               >  2:985900/1‑150 (MQ=255)

                                                         gCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACt                                                                                              >  1:1423418/1‑150 (MQ=255)

                                                           aaaTAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTcc                                                                                            >  2:846214/1‑150 (MQ=255)

                                                              taatTATTTGAAACAGGCCTGGAACAATATAAAAAATGAGTACGAAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCAt                                                                                          >  2:632376/1‑150 (MQ=255)

                                                               aatTATTTGAAACAGGCCTGGAACAATATAAAAAATGAGTACGAAAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATg                                                                                         <  1:632376/150‑1 (MQ=255)

                                                                   attTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTgg                                                                                    <  2:1544170/150‑1 (MQ=255)

                                                                   attTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTgg                                                                                    >  2:728442/1‑150 (MQ=255)

                                                                    tttGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGa                                                                                   >  2:964004/1‑150 (MQ=255)

                                                                    tttGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGa                                                                                   >  1:1239981/1‑150 (MQ=255)

                                                                      tGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAAt                                                                                 >  1:705699/1‑150 (MQ=255)

                                                                           cAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGaa                                                                            <  2:1690103/150‑1 (MQ=255)

                                                                           cAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGaa                                                                            <  1:985900/150‑1 (MQ=255)

                                                                           cAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGaa                                                                            <  1:616554/150‑1 (MQ=255)

                                                                            aGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAAt                                                                           <  2:36588/150‑1 (MQ=255)

                                                                                cTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACt                                                                       <  1:752413/150‑1 (MQ=255)

                                                                                 tGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTa                                                                      >  2:659454/1‑150 (MQ=255)

                                                                                    aaCGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTAAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaa                                                                   <  1:93829/150‑1 (MQ=255)

                                                                                      cGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaa                                                                 <  1:688958/150‑1 (MQ=255)

                                                                                       tatatAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAACGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaaa                                                                <  2:1056416/149‑1 (MQ=255)

                                                                                       gATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGaaaaa                                                                <  2:1244021/150‑1 (MQ=255)

                                                                                           taAAAAATGAGTTCA‑AATTATATCAATGATTTTCTATCACGGTTTTTTATATCACACTGGTGTTTATTATGCGGTTATACAATGATCTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGTAATGTGAATCACTAGAAAAAGAAt                                                            <  2:466384/150‑1 (MQ=255)

                                                                                           taAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAAt                                                            >  1:754532/1‑150 (MQ=255)

                                                                                               aaaTGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGa                                                        >  2:1325127/1‑150 (MQ=255)

                                                                                                 aTGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAgg                                                      >  2:526924/1‑150 (MQ=255)

                                                                                                  tGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGa                                                     >  2:724651/1‑150 (MQ=255)

                                                                                                  tGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGa                                                     <  2:678691/150‑1 (MQ=255)

                                                                                                    aGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGaaa                                                   >  1:1228092/1‑150 (MQ=255)

                                                                                                     gTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAAt                                                  >  1:420517/1‑150 (MQ=255)

                                                                                                      taag‑AAAAAAATCAAACATAATCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATTCAGAGGATACTCCATGTCTGGAATGTGAATCACCAGAAAAAGAATTTGAGGAAATg                                                 <  1:450933/147‑1 (MQ=255)

                                                                                                        cG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCa                                               >  2:1037296/1‑150 (MQ=255)

                                                                                                        cG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTAAACAATGAACTCAGACGTAAAGTAAATGATGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATTCa                                               >  2:1037299/1‑150 (MQ=255)

                                                                                                        actaaaataaTCAAACATATTAAATCACGATTTTTGAAAACACACTGGGGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGc                                                <  2:847258/147‑1 (MQ=255)

                                                                                                         g‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAATACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAg                                              >  1:1331700/1‑150 (MQ=255)

                                                                                                           aaaaaaaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATAAAAAGAACTCAGACGTAAATTAAAAGAAGAGGATACTCCATGTCTCGAATGTGAATCACTAGAAAAAGAATTTGAGAAAATCCAGa                                             >  1:1240116/1‑150 (MQ=255)

                                                                                                             aaaaaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaat                                           >  2:794039/1‑150 (MQ=255)

                                                                                                              aaaaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatg                                          <  1:846214/150‑1 (MQ=255)

                                                                                                                aaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgat                                        >  2:638922/1‑150 (MQ=255)

                                                                                                                aaTCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgat                                        <  2:1423418/150‑1 (MQ=255)

                                                                                                                  tCAAACATATTCAATCACGCTTTTTGTAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataa                                      <  1:1089456/150‑1 (MQ=255)

                                                                                                                    aaaCATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGaatgataatg                                    <  2:1594959/150‑1 (MQ=255)

                                                                                                                           ttCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATc                             >  2:1624682/1‑150 (MQ=255)

                                                                                                                             cAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCat                           >  1:439936/1‑150 (MQ=255)

                                                                                                                             cAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCat                           >  2:1009433/1‑150 (MQ=255)

                                                                                                                               aTCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCatta                         >  1:1580427/1‑150 (MQ=255)

                                                                                                                                 cACGCTTTTTGAAAACCCACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCattatt                       >  2:1318255/1‑150 (MQ=255)

                                                                                                                                 cACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTTAGGAAATGCTGAATGATAATGATCTATCattatt                       >  2:1689496/1‑150 (MQ=255)

                                                                                                                                 cACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCattatt                       >  2:1612779/1‑150 (MQ=255)

                                                                                                                                    gCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCAttatttat                    >  2:14921/1‑150 (MQ=255)

                                                                                                                                     cTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATAGATCattatcattc                   <  2:1228092/150‑6 (MQ=255)

                                                                                                                                      tttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCTGAATGATAATGATCTATCATTATTTATga                  <  1:1008351/150‑1 (MQ=255)

                                                                                                                                      tttttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATga                  >  1:1140247/1‑150 (MQ=255)

                                                                                                                                        gcataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATgaga                >  2:1366572/5‑150 (MQ=255)

                                                                                                                                         ttGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGaa               >  1:1258845/1‑150 (MQ=255)

                                                                                                                                         cataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCatta                         >  1:1608050/4‑140 (MQ=255)

                                                                                                                                         cataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCatta                         <  2:1608050/137‑1 (MQ=255)

                                                                                                                                          ataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAat              >  1:1497998/3‑150 (MQ=255)

                                                                                                                                          ataaaaCACACCAGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAat              >  2:415879/3‑150 (MQ=255)

                                                                                                                                           gAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAata             >  1:186854/1‑150 (MQ=255)

                                                                                                                                           gAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAata             <  1:993939/150‑1 (MQ=255)

                                                                                                                                            aaaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAatat            >  1:567654/1‑150 (MQ=255)

                                                                                                                                             aaaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATAtt           >  1:993964/1‑150 (MQ=255)

                                                                                                                                              aaCACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTg          <  2:314721/150‑1 (MQ=255)

                                                                                                                                               acactcTGGTGTGTTTTATGCGGGTATACAATCAACTCTGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGATAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGc         <  2:1645443/150‑1 (MQ=255)

                                                                                                                                                cacacTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCg        >  1:406246/1‑150 (MQ=255)

                                                                                                                                                      ggTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAATGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTaa  >  1:821767/1‑150 (MQ=255)

TATTAAATTTTATAGACGCTATATATGGGTAGTAATATACATGGAATTAGTTGCACTGCAAATAATTATTTGAAACAGGCCTGGAACGATATAAAAAATGAGTACG‑AAAAAAATCAAACATATTCAATCACGCTTTTTGAAAACACACTGGTGTGTTTTATGCGGTTATACAATGAACTCAGACGTAAAGTAAATGAAGAGGATACTCCATGTCTGGAATGTGAATCACTAGAAAAAGAATTTGAGGAAATGCAGAATGATAATGATCTATCATTATTTATGAGAATATTGCGTACTAA  >  NC_000913/1064012‑1064310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`