Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,070,878 T→A 5.0% T26T (ACA→ACT rutE ← putative malonic semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,070,8780TA 5.0% 298.8 / 2.6 106T26T (ACA→ACTrutEputative malonic semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (56/41);  new base A (3/3);  total (59/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CAGATTGCCGCTGGAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTAACAGAGCAT  >  NC_000913/1070731‑1071023
                                                                                                                                                   |                                                                                                                                                 
cAGATTGCCGCTGGAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGgtgt                                                                                                                                                 >  1:1531098/1‑150 (MQ=255)
  gATTGCCGCTGGAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGCCGGGTGtct                                                                                                                                               >  1:1386371/1‑150 (MQ=255)
  gATTGCCGCTGGAAAGTGCAGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGtct                                                                                                                                               >  1:87106/1‑150 (MQ=255)
   aTTGCCGCTGGAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGtctc                                                                                                                                              <  1:1534428/150‑1 (MQ=255)
      gCCGCTGGAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCg                                                                                                                                           <  1:610474/150‑1 (MQ=255)
       ccGCTGGAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGc                                                                                                                                          >  1:1493803/1‑150 (MQ=255)
           tGGAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGc                                                                                                                                      <  1:924789/150‑1 (MQ=255)
            ggAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTCACGTCTCCTCGATGACGGGTGTCTCCCGCCAGcc                                                                                                                                     >  2:309590/1‑150 (MQ=255)
            ggAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGATTGTAAACAAGATACGTGACGGAGAACAGTTAGCTTATGTCGGCCCCCATTTCAGCAGGGCATAAATCTACCGTAACGTCTCATCTCTGACGGGAGTCTCCCGcccggc                                                                                                                                     >  2:314292/1‑146 (MQ=255)
                aGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTtg                                                                                                                                 >  1:59928/1‑150 (MQ=255)
                 gTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTtgt                                                                                                                                >  2:1537083/1‑150 (MQ=255)
                 gTGCCGGACGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTtgt                                                                                                                                >  2:769909/1‑150 (MQ=255)
                  tGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTtgtg                                                                                                                               <  1:569321/150‑1 (MQ=255)
                  tGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTtgtg                                                                                                                               <  1:837490/150‑1 (MQ=255)
                   gCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGGCTCCCGCCAGCCGTTGTGa                                                                                                                              >  2:1373844/1‑150 (MQ=255)
                    ccGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAg                                                                                                                             >  2:620036/1‑150 (MQ=255)
                      gggCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTg                                                                                                                           >  2:64669/1‑150 (MQ=255)
                       ggCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGc                                                                                                                          >  2:731456/1‑150 (MQ=255)
                       ggCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGc                                                                                                                          >  1:1576244/1‑150 (MQ=255)
                         cgcAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCgg                                                                                                                        <  2:287131/150‑1 (MQ=255)
                         cgcAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCgg                                                                                                                        <  2:725752/150‑1 (MQ=255)
                           cAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCggcc                                                                                                                      >  2:66069/1‑148 (MQ=255)
                           cAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGc                                                                                                                      >  1:1404355/1‑150 (MQ=255)
                           cAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGc                                                                                                                      >  2:832941/1‑150 (MQ=255)
                           cAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGc                                                                                                                      <  2:1576244/150‑1 (MQ=255)
                                 ttCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGt                                                                                                                >  1:690708/1‑150 (MQ=255)
                                  tCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTg                                                                                                               <  2:87106/150‑1 (MQ=255)
                                   catttCCTTCAGCCCTCCGGGCAAACACGATCCGTGCCGGAGAACAGATAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCCTAACGTCTCTTCGGTGTCTGGTGTCTGGCGCCAGCCTTTGTCAGTGCGGGCATCGGTGa                                                                                                              <  2:489951/148‑1 (MQ=255)
                                     tttCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAAc                                                                                                            >  1:1434390/1‑150 (MQ=255)
                                        ccTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAgg                                                                                                         >  1:1025855/1‑150 (MQ=255)
                                             tGCCGTGCGGGTAAACACGATCCGTGTCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCTCTGACGGGTGTCTCCCGCCCGCGGGTGTGAGTGCGGGCATCGGTGAACAGGGTGCt                                                                                                    >  2:170855/1‑150 (MQ=255)
                                                cGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAg                                                                                                 >  2:1076979/1‑150 (MQ=255)
                                                  tGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAgcg                                                                                               <  2:495782/150‑1 (MQ=255)
                                                    cGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCa                                                                                             >  1:1115727/1‑150 (MQ=255)
                                                      ggTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTTAGCGCAcc                                                                                           >  1:346763/1‑150 (MQ=255)
                                                         aaaCACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTgg                                                                                        >  1:1301142/1‑150 (MQ=255)
                                                         aaaCACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGGGCGGGCAGCGGTGAACAGGGTGCTGAGCGCACCTgg                                                                                        >  2:854179/1‑150 (MQ=255)
                                                           acacGATCCGTGCCGGAGAACAGTTAGGTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGc                                                                                      <  1:159139/150‑1 (MQ=255)
                                                           acacGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGc                                                                                      <  2:59928/150‑1 (MQ=255)
                                                             acGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTa                                                                                    >  1:704830/1‑150 (MQ=255)
                                                                aTCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCGATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGTCGGGTGTCTCCCGCCAGCCGTTGTGAGTGCTGGCATAGGTGAACAGTGTGCTAAGCGCACCTGGGCTAACg                                                                                 <  1:88459/150‑1 (MQ=255)
                                                                aTCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACg                                                                                 <  1:731456/150‑1 (MQ=255)
                                                                aTCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACg                                                                                 <  2:1252247/150‑1 (MQ=255)
                                                                aTCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACg                                                                                 <  1:606412/150‑1 (MQ=255)
                                                                    gTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCtt                                                                             <  2:1025855/150‑1 (MQ=255)
                                                                      gCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCg                                                                           >  1:579712/1‑150 (MQ=255)
                                                                       ccGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGcgtcgt                                                                          >  2:1156926/1‑150 (MQ=255)
                                                                        cGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCGGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGtt                                                                         <  1:1246372/150‑1 (MQ=255)
                                                                         ggAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTc                                                                        >  1:805807/1‑150 (MQ=255)
                                                                          gagaACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGGGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCa                                                                       <  2:997597/150‑1 (MQ=255)
                                                                          gagaACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCCCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTGGTTCa                                                                       >  2:129858/1‑150 (MQ=255)
                                                                           agaACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCAt                                                                      >  1:3714/1‑150 (MQ=255)
                                                                            gaACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATa                                                                     <  1:1174369/150‑1 (MQ=255)
                                                                             aaCAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAg                                                                    <  1:1040278/150‑1 (MQ=255)
                                                                             aaCAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAg                                                                    >  1:648658/1‑150 (MQ=255)
                                                                              aCAGTTAGCTGATGTCGGCCCCGATTTCATCAGGGCATAAATCTCCCGTAACGTGTCATCGCTGACGGGTGTCTCCCGCCCGCCGTTGAGAGTGCGGGCATCGGTGGACAGGGTGCTATGCGCACCTGGGCTTGCGGCTTCGGTCATAGc                                                                   >  1:1051307/1‑150 (MQ=255)
                                                                                 gTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTACCGGCTTCGTTCAGAGCact                                                                >  1:1075635/1‑148 (MQ=255)
                                                                                 gTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAAt                                                                >  1:1309701/1‑150 (MQ=255)
                                                                                 gTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAAt                                                                >  2:251108/1‑150 (MQ=255)
                                                                                 gTTAGCTGATGTCGGCCCCCAATTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAAt                                                                <  1:987784/150‑1 (MQ=255)
                                                                                  ttAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAAtt                                                               <  1:769909/150‑1 (MQ=255)
                                                                                      cTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCtt                                                           <  1:620036/150‑1 (MQ=255)
                                                                                      cTGAAGTCGGCCCCCTTTTCATCAGGGCATAAATCTCCCGTAACGTCTCCTCGCTGACGGGTGTCTCCGGCCAGCCGTTGTGAGTGCGGGCATCTGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCtt                                                           <  1:243600/150‑1 (MQ=255)
                                                                                        gATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGAGGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTAc                                                         <  1:1580375/150‑1 (MQ=255)
                                                                                             cGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACCGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCGTACAGggc                                                    >  1:1247378/1‑150 (MQ=255)
                                                                                              ggCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACAGCTTCGTTCATAGCAATTCCTTACAGggcg                                                   <  2:1531098/150‑1 (MQ=255)
                                                                                                cccccATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGggcggc                                                 >  1:1296044/1‑150 (MQ=255)
                                                                                                   ccATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTc                                              >  1:1341351/1‑150 (MQ=255)
                                                                                                   ccATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTc                                              >  1:1652106/1‑150 (MQ=255)
                                                                                                     aTTTCATCAGGGCATAAATCTCCCGTCACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCAc                                            <  2:1543902/150‑1 (MQ=255)
                                                                                                     aTTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCAc                                            >  2:622112/1‑150 (MQ=255)
                                                                                                     aTTTCATCAGGGCATAAATATCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGAGCCGGCATCGGTGCACAGGGTGCTAAGAGCACCTGGTCTAACGGCTTCGTTCATAGCAATTCCTAACAGGTCGGCTTCAc                                            >  2:283152/1‑150 (MQ=255)
                                                                                                          atcaGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTTAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAAATCCTTACAGGGCGGCTTCCCGGGGa                                       >  2:1055606/1‑150 (MQ=255)
                                                                                                          atcaGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGa                                       >  2:824149/1‑150 (MQ=255)
                                                                                                             aGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATATCAATTCCTTACAGGGCGGCTTCACGGTGATGt                                    <  1:1579436/150‑1 (MQ=255)
                                                                                                              gggCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTa                                   >  2:789491/1‑150 (MQ=255)
                                                                                                                 caaaaaTCTCCCGGAACGTCTCAGCGCTGACGGGGGTCTCCCGCCAGCCGTTGTGAGGGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCGAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACa                                <  2:1024323/147‑1 (MQ=255)
                                                                                                                 cATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACa                                >  1:1549137/1‑150 (MQ=255)
                                                                                                                 cATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCCGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACa                                >  1:1476758/1‑150 (MQ=255)
                                                                                                                       tctcCCGTCACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACCGGGGGCTAAGCGCACATGGGCTAACGGCTTCGTTCATAGCAATTCCTTACATGGCGGCTTCACGGTGATGTAACAGGCTgg                          <  2:976123/150‑1 (MQ=255)
                                                                                                                        ctcCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGc                         >  2:1644218/1‑150 (MQ=255)
                                                                                                                          cccGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCaa                       >  2:414064/1‑150 (MQ=255)
                                                                                                                          cccGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCaa                       >  2:597013/1‑150 (MQ=255)
                                                                                                                                  gTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTg               >  1:728409/1‑150 (MQ=255)
                                                                                                                                  gTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTg               <  2:1582226/150‑1 (MQ=255)
                                                                                                                                  gTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTg               <  2:1238901/150‑1 (MQ=255)
                                                                                                                                  gTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGGTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTg               <  2:1301142/150‑1 (MQ=255)
                                                                                                                                   tctcATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTg                           >  1:1078691/1‑137 (MQ=255)
                                                                                                                                   tctcATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTg                           <  2:1078691/137‑1 (MQ=255)
                                                                                                                                    ctcATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAAAAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAg             >  2:824525/1‑150 (MQ=255)
                                                                                                                                      cATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTa           >  1:590544/1‑150 (MQ=255)
                                                                                                                                      cATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTa           <  2:1404355/150‑1 (MQ=255)
                                                                                                                                      cATCGCTGACGGGGGGCTCCCGCCAGCCGGTGGGTGGGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTa           <  2:973988/150‑1 (MQ=255)
                                                                                                                                       aTCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGTTGAACAGGGGGCTTCGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACCGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTaa          <  1:854179/150‑1 (MQ=255)
                                                                                                                                       aTCGCTGACGGGTGGCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTaa          <  1:82021/150‑1 (MQ=255)
                                                                                                                                         ggCTGGCGGGAGACACCCGTCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTAACa        >  2:1232768/2‑150 (MQ=255)
                                                                                                                                          gCTGGCGGGAGACACCCGTCAGCCGTTGTGAGTGCGGGAATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATTGCAATTCCTTACAGGGCGGCATCACGGTGATGTAACAGGCTGTCAAGCCCGTTGAGTAACag       >  2:87756/1‑150 (MQ=255)
                                                                                                                                          gCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTAACag       >  1:663443/1‑150 (MQ=255)
                                                                                                                                            tGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTAACagag     <  2:1386371/150‑1 (MQ=255)
                                                                                                                                               cGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTAGCAAGCCCGTTGAGTAACAGAGCAt  <  1:622112/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAGATTGCCGCTGGAAAGTGCCGGGCGCAGACGTTCTTTTCCTTCTGCCGTGCGGGTAAACACGATCCGTGCCGGAGAACAGTTAGCTGATGTCGGCCCCCATTTCATCAGGGCATAAATCTCCCGTAACGTCTCATCGCTGACGGGTGTCTCCCGCCAGCCGTTGTGAGTGCGGGCATCGGTGAACAGGGTGCTAAGCGCACCTGGGCTAACGGCTTCGTTCATAGCAATTCCTTACAGGGCGGCTTCACGGTGATGTAACAGGCTGGCAAGCCCGTTGAGTAACAGAGCAT  >  NC_000913/1070731‑1071023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: