Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,134,676 C→T 5.1% intergenic (+119/‑53) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,134,6760CT 5.1% 320.1 / 3.6 99intergenic (+119/‑53)flgF/flgGflagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
Reads supporting (aligned to +/- strand):  ref base C (54/39);  new base T (2/3);  total (56/43)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

Only 100 of 102 total aligned reads displayed.

CCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACG  >  NC_000913/1134527‑1134825
                                                                                                                                                     |                                                                                                                                                     
ccGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTAcgccgc                                                                                                                                                       >  1:1033927/1‑150 (MQ=255)
 cgggCCAACCAACTGCGGTCGATGTGTTACTTGAAAGGCTACATGACAAGTATAAGTTGCCCGATGCGCAAGGTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCa                                                                                                                                                      <  1:320260/147‑1 (MQ=39)
     ccaaccaaCTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                                                  <  1:492471/150‑1 (MQ=255)
     ccaaccaaCTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                                  >  2:694375/1‑150 (MQ=255)
     ccaaccaaCTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                                  >  2:692015/1‑150 (MQ=255)
      caaccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                                 >  1:813219/1‑150 (MQ=255)
       aaccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                                >  1:860580/1‑150 (MQ=255)
        accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                               <  2:510950/150‑2 (MQ=255)
        accaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                               <  2:754500/150‑2 (MQ=255)
         ccaacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCAGCATCCGGCaa                                                                                                                                              <  2:244375/150‑1 (MQ=255)
          caacTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCaag                                                                                                                                             >  2:970371/1‑150 (MQ=255)
               gctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                                        >  2:1095755/1‑150 (MQ=255)
               gctgTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGAc                                                                                                                                        >  1:1189901/1‑150 (MQ=255)
                  gTCGATGAGTTAATTGAAAGGATACGTGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACata                                                                                                                                     >  1:954032/1‑150 (MQ=255)
                  gTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACata                                                                                                                                     <  2:1453146/150‑1 (MQ=255)
                  gTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACata                                                                                                                                     <  1:1095755/150‑1 (MQ=255)
                    cGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATg                                                                                                                                   >  2:1012433/1‑150 (MQ=255)
                      aTGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCa                                                                                                                                 <  1:669869/150‑1 (MQ=255)
                          gTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGAACttt                                                                                                                             >  2:1140957/1‑150 (MQ=255)
                           ttAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTAATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTg                                                                                                                            <  1:1287517/150‑1 (MQ=255)
                            tAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATTTGCACTTTGt                                                                                                                           >  2:1117607/1‑150 (MQ=255)
                            tAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTATGt                                                                                                                           >  2:304573/1‑150 (MQ=255)
                             aaTTGAAAGGATACATGACAATTATAAGTTGCCCGATGCGCTAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTc                                                                                                                          <  1:314079/150‑1 (MQ=255)
                              aTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCa                                                                                                                         >  1:884798/1‑150 (MQ=255)
                              aTTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCa                                                                                                                         >  1:598415/1‑150 (MQ=255)
                               ttGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCAc                                                                                                                        <  1:1470145/150‑1 (MQ=255)
                                tGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTGTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACt                                                                                                                       >  2:1570833/1‑150 (MQ=255)
                                      gATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGGCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCa                                                                                                                 <  2:45167/150‑1 (MQ=255)
                                           aTGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACa                                                                                                            >  2:1352532/1‑150 (MQ=255)
                                            tGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAg                                                                                                           <  2:711146/150‑1 (MQ=255)
                                             gACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAgg                                                                                                          >  2:910585/1‑150 (MQ=255)
                                              aCAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCAGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGa                                                                                                         >  2:1232477/1‑150 (MQ=255)
                                               cAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGAc                                                                                                        >  2:254965/1‑150 (MQ=255)
                                                 aGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAt                                                                                                      >  2:1576680/1‑150 (MQ=255)
                                                 aGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACAt                                                                                                      >  1:643096/1‑150 (MQ=255)
                                                     taAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGTGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTa                                                                                                  <  1:429025/150‑1 (MQ=255)
                                                     taAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTa                                                                                                  >  2:1335883/1‑150 (MQ=255)
                                                         ttGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGAt                                                                                              <  2:936627/150‑1 (MQ=255)
                                                           gCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCa                                                                                            >  1:384031/1‑150 (MQ=255)
                                                           gCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCa                                                                                            >  1:1557838/1‑150 (MQ=255)
                                                             ccGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGt                                                                                          <  2:289816/150‑1 (MQ=255)
                                                              cGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGtt                                                                                         >  1:203118/1‑150 (MQ=255)
                                                              cGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGtt                                                                                         <  1:970371/150‑1 (MQ=255)
                                                                       aGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGa                                                                                >  1:62864/1‑150 (MQ=255)
                                                                        gTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGAt                                                                               >  1:388841/1‑150 (MQ=255)
                                                                         tttATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATc                                                                              <  1:181018/150‑1 (MQ=255)
                                                                         tttATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATc                                                                              <  1:595637/150‑1 (MQ=255)
                                                                           tATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGc                                                                            <  1:632290/150‑1 (MQ=255)
                                                                               gggTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaa                                                                        >  1:1699019/1‑150 (MQ=255)
                                                                               gggTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaa                                                                        >  1:1627293/1‑150 (MQ=255)
                                                                                ggTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCaaaa                                                                       <  2:445266/150‑1 (MQ=255)
                                                                                 gTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAAc                                                                      <  1:98298/150‑1 (MQ=255)
                                                                                    tATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACggg                                                                   <  2:1627293/150‑1 (MQ=255)
                                                                                    tATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACggg                                                                   >  2:1077039/1‑150 (MQ=255)
                                                                                       gggggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCt                                                                <  1:910585/150‑1 (MQ=255)
                                                                                       gggggCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCAAAAACGGGCCt                                                                <  2:954032/150‑1 (MQ=255)
                                                                                            cAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACg                                                           >  2:360431/1‑150 (MQ=255)
                                                                                              aTCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGcc                                                         >  2:104787/1‑150 (MQ=255)
                                                                                                  cAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGc                                                     <  1:692015/150‑1 (MQ=255)
                                                                                                        aTCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCa                                               <  1:973941/150‑1 (MQ=255)
                                                                                                        aTCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCa                                               >  1:256869/1‑150 (MQ=255)
                                                                                                         tCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCaa                                              >  1:746508/1‑150 (MQ=255)
                                                                                                          cGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAat                                             >  1:872025/1‑150 (MQ=255)
                                                                                                           gATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAata                                            <  1:1981/150‑1 (MQ=255)
                                                                                                              tttGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATgg                                         <  1:1012433/150‑1 (MQ=255)
                                                                                                                  cGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGt                                     >  1:1714800/1‑150 (MQ=255)
                                                                                                                  cGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGt                                     >  2:902317/1‑150 (MQ=255)
                                                                                                                      cACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCAtt                                 <  2:813219/150‑1 (MQ=255)
                                                                                                                        cTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGc                               <  2:388841/150‑1 (MQ=255)
                                                                                                                         ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGcc                              <  2:390030/150‑1 (MQ=255)
                                                                                                                         ttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGcc                              <  1:1352532/150‑1 (MQ=255)
                                                                                                                          tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCca                             >  2:1080196/1‑150 (MQ=255)
                                                                                                                            tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaac                           >  2:501898/1‑150 (MQ=255)
                                                                                                                              tgCCGGATGCGGCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaacaa                         >  1:1406814/2‑150 (MQ=255)
                                                                                                                              ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaacaa                         >  2:1614675/1‑150 (MQ=255)
                                                                                                                              ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaacaa                         >  2:1608367/1‑150 (MQ=255)
                                                                                                                              ggCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCcaacaa                         >  1:784297/1‑150 (MQ=255)
                                                                                                                                ccGGATGCGGCGTAAACGCCTTATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc                       >  1:338029/1‑150 (MQ=255)
                                                                                                                                ccGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAAcc                       >  1:316740/1‑150 (MQ=255)
                                                                                                                                 cGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATATTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCt                      >  2:1535140/1‑150 (MQ=255)
                                                                                                                                  ggATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACATGACATTTTATGATCAGTTCATTATGGATCACCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTg                     <  2:1033927/150‑1 (MQ=255)
                                                                                                                                   gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTgg                    <  2:1049761/150‑1 (MQ=255)
                                                                                                                                   gATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTgg                    >  1:959743/1‑150 (MQ=255)
                                                                                                                                    aTAATGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGc                   <  2:860580/150‑1 (MQ=255)
                                                                                                                                    aTAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGc                   <  2:1422308/150‑1 (MQ=255)
                                                                                                                                     tAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCa                  >  2:34200/1‑150 (MQ=255)
                                                                                                                                     tAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCa                  <  1:1232477/150‑1 (MQ=255)
                                                                                                                                       aGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCaaa                >  1:743622/1‑150 (MQ=255)
                                                                                                                                       aGGCGTTTACGCCGCATCAGGCAAGAAGACATATGCCCTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCaaa                >  1:204656/1‑150 (MQ=255)
                                                                                                                                         gCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACg              >  2:1711567/1‑150 (MQ=255)
                                                                                                                                         gCGTTTACGCCGAATCCGGCAAGAAGGCATATGCACTTTGTCACTAATCCACTACAGGACTTTTTATGTTCAGTTCTTTATTGATCGCCAAAACTGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCTACAACCTGGCAAACg              <  2:1345336/150‑1 (MQ=255)
                                                                                                                                          cGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGt             <  2:884798/150‑1 (MQ=255)
                                                                                                                                          cGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGt             >  1:1602859/1‑150 (MQ=255)
                                                                                                                                          cGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGt             <  2:598415/150‑1 (MQ=255)
                                                                                                                                          cGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCACCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGt             >  2:1151282/1‑150 (MQ=255)
                                                                                                                                           gTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTc            >  1:1397882/1‑150 (MQ=255)
                                                                                                                                             ttACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAg          <  2:1626766/150‑1 (MQ=255)
                                                                                                                                             ttACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAg          <  2:962662/150‑1 (MQ=255)
                                                                                                                                             tcttgccggATGCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAg          >  1:1197154/5‑150 (MQ=255)
                                                                                                                                                     cATCCGGCAAGAAGACATATGCACTTTGTCACTACTCCACTACAGGACATTTTATGATCAGATCAATATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACg  <  1:1028652/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCACTTTGTCACTAATCCACTACAGGACATTTTATGATCAGTTCATTATGGATCGCCAAAACGGGCCTTGACGCCCAGCAAACCAATATGGACGTCATTGCCAACAACCTGGCAAACGTCAGTACTAACG  >  NC_000913/1134527‑1134825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: