Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,136,026 G→T 10.1% V155L (GTA→TTA) ‡ flgH → flagellar protein of basal‑body outer‑membrane L ring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,136,0260GT10.1% 276.6 / 16.9 96V155L (GTA→TTA) ‡flgHflagellar protein of basal‑body outer‑membrane L ring
Reads supporting (aligned to +/- strand):  ref base G (36/49);  new base T (5/5);  total (42/54)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

GCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCG  >  NC_000913/1135877‑1136174
                                                                                                                                                     |                                                                                                                                                    
gCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAgg                                                                                                                                                      <  2:1190944/150‑1 (MQ=255)
gCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAgg                                                                                                                                                      <  2:1144849/150‑1 (MQ=255)
gCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTATGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAgg                                                                                                                                                      <  1:1325682/150‑1 (MQ=255)
 cTTTGATACTGTGCCGCGCTATTTGCCGCGGCTGTTTGGTAACGCTCGGGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGt                                                                                                                                                     <  1:1542796/150‑1 (MQ=255)
 cTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTGCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGt                                                                                                                                                     <  1:1078772/150‑1 (MQ=255)
 cTTTGAGACTGTGCCGCGCTATTTGCAGGGGCGGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACTTTGACGGTGACGGTTGACCAGGt                                                                                                                                                     <  2:1548677/150‑1 (MQ=255)
    tGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACt                                                                                                                                                  >  2:1369809/1‑150 (MQ=255)
    tGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACt                                                                                                                                                  <  1:1544010/150‑1 (MQ=255)
     gATACTGTGCCTCGCTAGTTGCAGGGGCGGTTTGGTAACGCTCGTGCCGCTGGCGCAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTg                                                                                                                                                 <  2:1118507/150‑1 (MQ=255)
     gATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTg                                                                                                                                                 >  1:1578951/1‑150 (MQ=255)
        aCTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCTATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTc                                                                                                                                              <  1:309839/150‑1 (MQ=255)
         cTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCa                                                                                                                                             >  1:1149445/1‑150 (MQ=255)
           gtgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAc                                                                                                                                           <  1:807866/150‑1 (MQ=255)
            tgCCGCGCTATTTGCAGTGGCGGTTTGGTAACGCTCGTGCCGATGCCGACGCCGCCGGTGGGAACCCGTTCCACGGAACGGGCGGGGCCAAGGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAcc                                                                                                                                          <  1:93941/150‑2 (MQ=255)
            tgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACg                                                                                                                                          >  1:626806/1‑150 (MQ=255)
            tgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGGGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAcc                                                                                                                                          <  2:1429611/150‑2 (MQ=255)
            ggCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCGCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAcc                                                                                                                                          <  1:880776/149‑2 (MQ=255)
             ggcgcgTTTTTTGCATGGGCTTTTTGGTTACTGTCGTGCCGTTTTCTTATCCTCCGGTGGTAACCCGTTCCACGGAAATGTGGGTGCCAATGCAAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACgg                                                                                                                                         <  2:111058/148‑1 (MQ=255)
             gCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACgg                                                                                                                                         <  1:12978/150‑1 (MQ=255)
             gCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGGAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAccg                                                                                                                                         <  1:297588/150‑3 (MQ=255)
                  gcTATTTGCAGGGGCTGTTTGGTCACGCTCGTGCCGAAGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCTGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGACAACGGCAAcc                                                                                                                                    <  2:253190/150‑1 (MQ=255)
                  gcTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAAcc                                                                                                                                    <  2:799461/150‑1 (MQ=255)
                  gcTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAAcc                                                                                                                                    <  2:525619/150‑1 (MQ=255)
                  gcTATTTGCAGGGGCTGGTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGGTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAAcc                                                                                                                                    <  2:901197/150‑1 (MQ=255)
                   cTAATTGCCGGGGCTGTTTGGTCACGCTCGGGCCGATGTCTAAGACTCCGGCGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAAAACCTTAAGCGGCACGTTGACGGTGACGTTTGACCAGGTACTGGTCAACGGCAACCt                                                                                                                                   <  1:948943/150‑1 (MQ=255)
                          cAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTGGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCAtgtg                                                                                                                            >  1:565358/1‑150 (MQ=255)
                             gggCTGTTTGGTAACGCGCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATgtggtg                                                                                                                         <  1:128971/150‑1 (MQ=255)
                              ggCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGGGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGtggtgg                                                                                                                        <  1:1370798/150‑1 (MQ=255)
                                cTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTggtgggt                                                                                                                      >  2:436877/1‑150 (MQ=255)
                                 tGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTggtgggtg                                                                                                                     <  2:678397/150‑1 (MQ=255)
                                    ttGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGaaa                                                                                                                  <  2:1198645/150‑1 (MQ=255)
                                        tAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACa                                                                                                              >  1:339864/1‑150 (MQ=255)
                                        tAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACa                                                                                                              <  2:400725/150‑1 (MQ=255)
                                            gCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGAtt                                                                                                          <  2:652031/150‑1 (MQ=255)
                                               cGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGcc                                                                                                       >  1:422465/1‑150 (MQ=255)
                                                    cGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTaa                                                                                                  >  1:959955/1‑150 (MQ=255)
                                                      caggcgcaGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATc                                                                                                <  1:674701/143‑1 (MQ=255)
                                                      atttCGACGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATc                                                                                                <  1:1318793/147‑1 (MQ=255)
                                                      atatCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGCCCAGGTACTGGTCAACGGCAACCTGCAGGTGGTGGGTGAACAACAGATTGCCATTAATc                                                                                                >  2:1505972/4‑150 (MQ=255)
                                                      aTGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATc                                                                                                >  2:678139/1‑150 (MQ=255)
                                                      aTGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATc                                                                                                <  2:757597/150‑1 (MQ=255)
                                                       tGTCGCAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCa                                                                                               <  1:458307/150‑1 (MQ=255)
                                                        gTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAg                                                                                              <  2:172566/150‑1 (MQ=255)
                                                         tCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAgg                                                                                             <  2:959955/150‑1 (MQ=255)
                                                          cgcatccTCCGGTGGTAACACGTTCAACGGAAAGGGCGGTGCCAATGCCAGCAATACCTTTAGCGTCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGTCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAggg                                                                                            <  2:152670/145‑1 (MQ=255)
                                                          cGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAggg                                                                                            >  1:55727/1‑150 (MQ=255)
                                                          cGAAGCCTACGGTGGAAACAAGTTCAACGTAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAggg                                                                                            <  2:169066/150‑1 (MQ=255)
                                                             tgCCTCCGGTGGTCACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTAc                                                                                         <  2:60780/149‑1 (MQ=255)
                                                                   cGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAAtt                                                                                   <  2:401328/150‑1 (MQ=255)
                                                                   cGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAAtt                                                                                   <  2:1149445/150‑1 (MQ=255)
                                                                   cGGTGGAAACACGTTCAACCGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTTACCAGCAACTGGTCAACGGCAACCTGCATGTGGTGGGTCAAAAACAGATTGCCATTAATCAGGCTACCGaaat                                                                                   >  2:1648045/1‑148 (MQ=255)
                                                                    ggtggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTAAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAAttt                                                                                  <  1:213177/150‑1 (MQ=255)
                                                                     gtggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTa                                                                                 <  2:422465/150‑1 (MQ=255)
                                                                     gtggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTa                                                                                 <  2:885937/150‑1 (MQ=255)
                                                                      tggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTAt                                                                                >  1:749000/1‑150 (MQ=255)
                                                                       ggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTAtt                                                                               >  2:1377470/1‑150 (MQ=255)
                                                                        gtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACAAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTc                                                                              >  1:562804/1‑150 (MQ=255)
                                                                         tAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCg                                                                             >  2:752606/1‑150 (MQ=255)
                                                                             acGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTtc                                                                         <  2:1100511/150‑1 (MQ=255)
                                                                              cGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTtct                                                                        >  2:529145/1‑150 (MQ=255)
                                                                                 tCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTg                                                                     <  2:1578951/150‑1 (MQ=255)
                                                                                  cAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTgg                                                                    <  2:463541/150‑1 (MQ=255)
                                                                                    aCGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGCCGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCg                                                                  >  1:1077501/1‑150 (MQ=255)
                                                                                     cGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGt                                                                 >  1:469525/1‑150 (MQ=255)
                                                                                       gAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCCGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTgg                                                               >  1:389696/1‑150 (MQ=255)
                                                                                          aGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGCCGGTGCCGGTTGACCAGGTACTGGTCAACGGCCAGCTGCATGTGGTGGGTGAAAAACAGATTGCCATTATTCAGGGTTCCGAATTTATTCGCTTCTCTGGCGTGGTTa                                                            >  2:1634629/1‑150 (MQ=255)
                                                                                             gCGGGGGCAATGCCAGCAATACCTTTTGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATc                                                         <  1:569092/150‑1 (MQ=255)
                                                                                             gCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATc                                                         >  2:833909/1‑150 (MQ=255)
                                                                                                 ggCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACg                                                     <  2:936574/150‑1 (MQ=255)
                                                                                                       tGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTAt                                               >  2:1006342/1‑150 (MQ=255)
                                                                                                       tGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTAt                                               >  2:480424/1‑150 (MQ=255)
                                                                                                        gCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATc                                              >  1:1212467/1‑150 (MQ=255)
                                                                                                           aGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCCGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGc                                           <  2:389696/150‑1 (MQ=255)
                                                                                                            gCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCg                                          >  1:366408/1‑150 (MQ=255)
                                                                                                             cAATACCTTTAGCGGCACGTTGACGGTGACGGTTGCCCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCgg                                         >  1:185886/1‑150 (MQ=255)
                                                                                                              aaTACCTTTAGCGGCACGTTGACGGTGACTGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGgc                                        <  1:35686/150‑1 (MQ=255)
                                                                                                                  tcgacatcGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGgcagca                                    <  2:902229/143‑1 (MQ=255)
                                                                                                                   cTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCaa                                   <  2:694289/150‑1 (MQ=255)
                                                                                                                       aGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtacc                               >  2:1108272/1‑150 (MQ=255)
                                                                                                                          ggCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccgta                            <  1:993198/150‑1 (MQ=255)
                                                                                                                           gCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccgtac                           >  1:514985/1‑150 (MQ=255)
                                                                                                                            cACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATc                                              >  1:1030661/1‑130 (MQ=255)
                                                                                                                            cACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATc                                              <  2:1030661/130‑1 (MQ=255)
                                                                                                                             aCGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccgtaccg                         <  2:1077501/150‑1 (MQ=255)
                                                                                                                                    cggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTc                  >  2:1548571/1‑150 (MQ=255)
                                                                                                                                    cggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCAACGCACTATCAGCGGCAGCAATACCGGACCGTCTtctc                  >  2:232052/1‑150 (MQ=255)
                                                                                                                                     ggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCa                 >  2:1294111/1‑150 (MQ=255)
                                                                                                                                     ggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCa                 <  1:1127238/150‑1 (MQ=255)
                                                                                                                                      gtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCTGCGGCAGCATTACCGTCCCGGCTACTCAg                >  1:279821/1‑150 (MQ=255)
                                                                                                                                         gccgtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTg             >  1:710686/4‑150 (MQ=255)
                                                                                                                                         acggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTg             >  1:988498/1‑150 (MQ=255)
                                                                                                                                          ccgtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCTTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTgg            >  1:332057/3‑150 (MQ=255)
                                                                                                                                          ccgtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTgg            >  1:777993/3‑150 (MQ=255)
                                                                                                                                           cgtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGc           >  1:1694826/2‑150 (MQ=255)
                                                                                                                                            gtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCg          >  1:410768/1‑150 (MQ=255)
                                                                                                                                            gtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGTTGAAAAACAGATTGCCATTAATCAGTGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCCCGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCg          <  2:316985/150‑1 (MQ=255)
                                                                                                                                            gtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCg          <  1:1369809/150‑1 (MQ=255)
                                                                                                                                            gtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGGGGGTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCg          <  2:869693/150‑1 (MQ=255)
                                                                                                                                                    ggTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATgcgcg  >  1:1620023/1‑150 (MQ=255)
                                                                                                                                                    ggTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATgcgcg  >  1:178854/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCTTTGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCG  >  NC_000913/1135877‑1136174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: