Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,136,028 A→C 10.3% V155V (GTA→GTC) ‡ flgH → flagellar protein of basal‑body outer‑membrane L ring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,136,0280AC10.3% 260.1 / 17.8 95V155V (GTA→GTC) ‡flgHflagellar protein of basal‑body outer‑membrane L ring
Reads supporting (aligned to +/- strand):  ref base A (39/46);  new base C (5/5);  total (44/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

TGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCAT  >  NC_000913/1135881‑1136177
                                                                                                                                                   |                                                                                                                                                     
tGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACt                                                                                                                                                     <  1:1544010/150‑1 (MQ=255)
tGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACt                                                                                                                                                     >  2:1369809/1‑150 (MQ=255)
 gATACTGTGCCTCGCTAGTTGCAGGGGCGGTTTGGTAACGCTCGTGCCGCTGGCGCAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTg                                                                                                                                                    <  2:1118507/150‑1 (MQ=255)
 gATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTg                                                                                                                                                    >  1:1578951/1‑150 (MQ=255)
    aCTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCTATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTc                                                                                                                                                 <  1:309839/150‑1 (MQ=255)
     cTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCa                                                                                                                                                >  1:1149445/1‑150 (MQ=255)
       gtgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAc                                                                                                                                              <  1:807866/150‑1 (MQ=255)
        tgCCGCGCTATTTGCAGTGGCGGTTTGGTAACGCTCGTGCCGATGCCGACGCCGCCGGTGGGAACCCGTTCCACGGAACGGGCGGGGCCAAGGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAcc                                                                                                                                             <  1:93941/150‑2 (MQ=255)
        tgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACg                                                                                                                                             >  1:626806/1‑150 (MQ=255)
        tgCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGGGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAcc                                                                                                                                             <  2:1429611/150‑2 (MQ=255)
        ggCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCGCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAcc                                                                                                                                             <  1:880776/149‑2 (MQ=255)
         ggcgcgTTTTTTGCATGGGCTTTTTGGTTACTGTCGTGCCGTTTTCTTATCCTCCGGTGGTAACCCGTTCCACGGAAATGTGGGTGCCAATGCAAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACgg                                                                                                                                            <  2:111058/148‑1 (MQ=255)
         gCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACgg                                                                                                                                            <  1:12978/150‑1 (MQ=255)
         gCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGGAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGTACCTGGTCAAccg                                                                                                                                            <  1:297588/150‑3 (MQ=255)
              gcTATTTGCAGGGGCTGTTTGGTCACGCTCGTGCCGAAGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCTGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGACAACGGCAAcc                                                                                                                                       <  2:253190/150‑1 (MQ=255)
              gcTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAAcc                                                                                                                                       <  2:799461/150‑1 (MQ=255)
              gcTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAAcc                                                                                                                                       <  2:525619/150‑1 (MQ=255)
              gcTATTTGCAGGGGCTGGTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGGTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAAcc                                                                                                                                       <  2:901197/150‑1 (MQ=255)
               cTAATTGCCGGGGCTGTTTGGTCACGCTCGGGCCGATGTCTAAGACTCCGGCGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAAAACCTTAAGCGGCACGTTGACGGTGACGTTTGACCAGGTACTGGTCAACGGCAACCt                                                                                                                                      <  1:948943/150‑1 (MQ=255)
                      cAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTGGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCAtgtg                                                                                                                               >  1:565358/1‑150 (MQ=255)
                         gggCTGTTTGGTAACGCGCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATgtggtg                                                                                                                            <  1:128971/150‑1 (MQ=255)
                          ggCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGGGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGtggtgg                                                                                                                           <  1:1370798/150‑1 (MQ=255)
                            cTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTggtgggt                                                                                                                         >  2:436877/1‑150 (MQ=255)
                             tGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTggtgggtg                                                                                                                        <  2:678397/150‑1 (MQ=255)
                                ttGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGaaa                                                                                                                     <  2:1198645/150‑1 (MQ=255)
                                    tAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACa                                                                                                                 >  1:339864/1‑150 (MQ=255)
                                    tAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACa                                                                                                                 <  2:400725/150‑1 (MQ=255)
                                        gCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGAtt                                                                                                             <  2:652031/150‑1 (MQ=255)
                                           cGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGcc                                                                                                          >  1:422465/1‑150 (MQ=255)
                                                cGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTaa                                                                                                     >  1:959955/1‑150 (MQ=255)
                                                  caggcgcaGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATc                                                                                                   <  1:674701/143‑1 (MQ=255)
                                                  atttCGACGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATc                                                                                                   <  1:1318793/147‑1 (MQ=255)
                                                  atatCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGCCCAGGTACTGGTCAACGGCAACCTGCAGGTGGTGGGTGAACAACAGATTGCCATTAATc                                                                                                   >  2:1505972/4‑150 (MQ=255)
                                                  aTGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATc                                                                                                   >  2:678139/1‑150 (MQ=255)
                                                  aTGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATc                                                                                                   <  2:757597/150‑1 (MQ=255)
                                                   tGTCGCAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCa                                                                                                  <  1:458307/150‑1 (MQ=255)
                                                    gTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAg                                                                                                 <  2:172566/150‑1 (MQ=255)
                                                    gTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAg                                                                                                 >  2:993198/1‑150 (MQ=255)
                                                     tCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAgg                                                                                                <  2:959955/150‑1 (MQ=255)
                                                      cgcatccTCCGGTGGTAACACGTTCAACGGAAAGGGCGGTGCCAATGCCAGCAATACCTTTAGCGTCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGTCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAggg                                                                                               <  2:152670/145‑1 (MQ=255)
                                                      cGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAggg                                                                                               >  1:55727/1‑150 (MQ=255)
                                                      cGAAGCCTACGGTGGAAACAAGTTCAACGTAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAggg                                                                                               <  2:169066/150‑1 (MQ=255)
                                                         tgCCTCCGGTGGTCACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTAc                                                                                            <  2:60780/149‑1 (MQ=255)
                                                               cGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAAtt                                                                                      <  2:401328/150‑1 (MQ=255)
                                                               cGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAAtt                                                                                      <  2:1149445/150‑1 (MQ=255)
                                                               cGGTGGAAACACGTTCAACCGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTTACCAGCAACTGGTCAACGGCAACCTGCATGTGGTGGGTCAAAAACAGATTGCCATTAATCAGGCTACCGaaat                                                                                      >  2:1648045/1‑148 (MQ=255)
                                                                ggtggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTAAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAAttt                                                                                     <  1:213177/150‑1 (MQ=255)
                                                                 gtggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTa                                                                                    <  2:422465/150‑1 (MQ=255)
                                                                 gtggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTa                                                                                    <  2:885937/150‑1 (MQ=255)
                                                                  tggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTAt                                                                                   >  1:749000/1‑150 (MQ=255)
                                                                   ggtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTAtt                                                                                  >  2:1377470/1‑150 (MQ=255)
                                                                    gtAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACAAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTc                                                                                 >  1:562804/1‑150 (MQ=255)
                                                                     tAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCg                                                                                >  2:752606/1‑150 (MQ=255)
                                                                         acGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTtc                                                                            <  2:1100511/150‑1 (MQ=255)
                                                                          cGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTtct                                                                           >  2:529145/1‑150 (MQ=255)
                                                                             tCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTg                                                                        <  2:1578951/150‑1 (MQ=255)
                                                                              cAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTgg                                                                       <  2:463541/150‑1 (MQ=255)
                                                                                aCGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGCCGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCg                                                                     >  1:1077501/1‑150 (MQ=255)
                                                                                 cGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGt                                                                    >  1:469525/1‑150 (MQ=255)
                                                                                   gAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCCGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTgg                                                                  >  1:389696/1‑150 (MQ=255)
                                                                                      aGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGCCGGTGCCGGTTGACCAGGTACTGGTCAACGGCCAGCTGCATGTGGTGGGTGAAAAACAGATTGCCATTATTCAGGGTTCCGAATTTATTCGCTTCTCTGGCGTGGTTa                                                               >  2:1634629/1‑150 (MQ=255)
                                                                                         gCGGGGGCAATGCCAGCAATACCTTTTGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATc                                                            <  1:569092/150‑1 (MQ=255)
                                                                                         gCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATc                                                            >  2:833909/1‑150 (MQ=255)
                                                                                             ggCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACg                                                        <  2:936574/150‑1 (MQ=255)
                                                                                                   tGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTAt                                                  >  2:1006342/1‑150 (MQ=255)
                                                                                                   tGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTAt                                                  >  2:480424/1‑150 (MQ=255)
                                                                                                    gCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATc                                                 >  1:1212467/1‑150 (MQ=255)
                                                                                                       aGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCCGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGc                                              <  2:389696/150‑1 (MQ=255)
                                                                                                        gCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCg                                             >  1:366408/1‑150 (MQ=255)
                                                                                                         cAATACCTTTAGCGGCACGTTGACGGTGACGGTTGCCCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCgg                                            >  1:185886/1‑150 (MQ=255)
                                                                                                          aaTACCTTTAGCGGCACGTTGACGGTGACTGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGgc                                           <  1:35686/150‑1 (MQ=255)
                                                                                                              tcgacatcGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGgcagca                                       <  2:902229/143‑1 (MQ=255)
                                                                                                               cTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCaa                                      <  2:694289/150‑1 (MQ=255)
                                                                                                                   aGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtacc                                  >  2:1108272/1‑150 (MQ=255)
                                                                                                                      ggCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccgta                               <  1:993198/150‑1 (MQ=255)
                                                                                                                       gCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccgtac                              >  1:514985/1‑150 (MQ=255)
                                                                                                                        cACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATc                                                 >  1:1030661/1‑130 (MQ=255)
                                                                                                                        cACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATc                                                 <  2:1030661/130‑1 (MQ=255)
                                                                                                                         aCGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAAtaccgtaccg                            <  2:1077501/150‑1 (MQ=255)
                                                                                                                                cggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTc                     >  2:1548571/1‑150 (MQ=255)
                                                                                                                                cggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCAACGCACTATCAGCGGCAGCAATACCGGACCGTCTtctc                     >  2:232052/1‑150 (MQ=255)
                                                                                                                                 ggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCa                    <  1:1127238/150‑1 (MQ=255)
                                                                                                                                 ggtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCa                    >  2:1294111/1‑150 (MQ=255)
                                                                                                                                  gtgacggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCTGCGGCAGCATTACCGTCCCGGCTACTCAg                   >  1:279821/1‑150 (MQ=255)
                                                                                                                                     gccgtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTg                >  1:710686/4‑150 (MQ=255)
                                                                                                                                     acggtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTg                >  1:988498/1‑150 (MQ=255)
                                                                                                                                      ccgtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCTTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTgg               >  1:332057/3‑150 (MQ=255)
                                                                                                                                      ccgtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTgg               >  1:777993/3‑150 (MQ=255)
                                                                                                                                       cgtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGc              >  1:1694826/2‑150 (MQ=255)
                                                                                                                                        gtTGACCAGTACCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCg             >  1:410768/1‑150 (MQ=255)
                                                                                                                                        gtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGTTGAAAAACAGATTGCCATTAATCAGTGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCCCGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCg             <  2:316985/150‑1 (MQ=255)
                                                                                                                                        gtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCg             <  1:1369809/150‑1 (MQ=255)
                                                                                                                                        gtTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGGGGGTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCg             <  2:869693/150‑1 (MQ=255)
                                                                                                                                                ggTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATgcgcg     >  1:1620023/1‑150 (MQ=255)
                                                                                                                                                ggTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATgcgcg     >  1:178854/1‑150 (MQ=255)
                                                                                                                                                   aCTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCAt  >  2:978782/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGATACTGTGCCGCGCTATTTGCAGGGGCTGTTTGGTAACGCTCGTGCCGATGTCGAAGCCTCCGGTGGTAACACGTTCAACGGAAAGGGCGGGGCCAATGCCAGCAATACCTTTAGCGGCACGTTGACGGTGACGGTTGACCAGGTACTGGTCAACGGCAACCTGCATGTGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCAT  >  NC_000913/1135881‑1136177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: