Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,150,624 A→T 5.6% E299D (GAA→GAT fabD → malonyl‑CoA‑[acyl‑carrier‑protein] transacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,150,6240AT 5.6% 257.7 / 4.4 99E299D (GAA→GATfabDmalonyl‑CoA‑[acyl‑carrier‑protein] transacylase
Reads supporting (aligned to +/- strand):  ref base A (46/46);  new base T (3/3);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 101 total aligned reads displayed.

GTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAGTTATTGGCAC  >  NC_000913/1150480‑1150773
                                                                                                                                                |                                                                                                                                                     
gTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTc                                                                                                                                                  <  1:485601/150‑1 (MQ=255)
   taACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGc                                                                                                                                               <  1:1673669/150‑1 (MQ=255)
   taACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGc                                                                                                                                               >  1:585763/1‑150 (MQ=255)
     aCCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGa                                                                                                                                             >  1:1320125/1‑150 (MQ=255)
      cccGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGAt                                                                                                                                            >  2:1314409/1‑150 (MQ=255)
        cGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATgg                                                                                                                                          <  2:721816/150‑1 (MQ=255)
        cGGTGCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATgg                                                                                                                                          <  2:726070/150‑1 (MQ=255)
         ggTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGc                                                                                                                                         >  2:961286/1‑150 (MQ=255)
          gTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAAGGTTCGTTCAGCgccgagg                                                                                                                                        <  1:611921/150‑7 (MQ=255)
          gTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAAGGTTCGTTCAGCgccgagg                                                                                                                                        <  1:1392553/150‑7 (MQ=255)
            tCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAgc                                                                                                                                      <  1:600798/150‑1 (MQ=255)
             cAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAgcg                                                                                                                                     <  1:1469062/150‑1 (MQ=255)
              aGTGGACGAAGTCTGTTGAGTACATTGCAGCGCAAGGCGTAGAACTTCTCTATGTAGTCGGCCCGGGAAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGATCCTTCAGCGATGGCAgcgg                                                                                                                                    <  1:1254550/150‑1 (MQ=255)
              aGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAgcgg                                                                                                                                    >  1:750447/1‑150 (MQ=255)
                 ggACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGgcgc                                                                                                                                 <  1:1493604/150‑1 (MQ=255)
                    cGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCg                                                                                                                              <  2:1322940/150‑1 (MQ=255)
                    cGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGAGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCCGCGATGGCAGCGGCGCTCg                                                                                                                              >  2:704073/1‑150 (MQ=255)
                    cGAAGTCTGTGGAGTACATGCCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACTAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCg                                                                                                                              <  2:1412960/150‑1 (MQ=255)
                        gTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCt                                                                                                                          >  2:1720172/1‑150 (MQ=255)
                        gTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCt                                                                                                                          >  2:367978/1‑150 (MQ=255)
                         tCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCtt                                                                                                                         >  2:822072/1‑150 (MQ=255)
                           tGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTa                                                                                                                       >  2:943142/1‑150 (MQ=255)
                            gTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTaa                                                                                                                      >  1:552818/1‑150 (MQ=255)
                             ttGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTaaa                                                                                                                     <  2:448189/150‑1 (MQ=255)
                             gtgcgtccATGGCACCGCAAGCCGTAGAACATCTCAATGAAGTCGGCCCGGGCAAAGGGCAGACTCGCCGGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTaaa                                                                                                                     <  1:302116/143‑1 (MQ=255)
                              tGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTaaaa                                                                                                                    <  1:1482664/150‑1 (MQ=255)
                               gAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAag                                                                                                                   <  2:1071214/150‑1 (MQ=255)
                                aGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGACCCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAaga                                                                                                                  >  2:1397913/1‑150 (MQ=255)
                                 gTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAagag                                                                                                                 <  1:859519/150‑1 (MQ=255)
                                 gTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAagag                                                                                                                 <  2:1181477/150‑1 (MQ=255)
                                 gTACATGGCAGCGCAAGGCGGAGAACATCGCTATGCAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAagag                                                                                                                 <  2:710828/150‑1 (MQ=255)
                                  tACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAgg                                                                                                                >  1:1504784/1‑150 (MQ=255)
                                   aCATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGa                                                                                                               >  1:1202593/1‑150 (MQ=255)
                                    cATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGaa                                                                                                              >  2:1548634/1‑150 (MQ=255)
                                    cATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGaa                                                                                                              >  2:586703/1‑150 (MQ=255)
                                    cATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGAAGGCAGCGGCGCTCGAGCTTTAAAAGAGGaa                                                                                                              <  2:371512/150‑1 (MQ=255)
                                       ggCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGCCACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAAt                                                                                                           >  1:251232/1‑150 (MQ=255)
                                       ggCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAAt                                                                                                           >  2:395135/1‑150 (MQ=255)
                                         cAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCa                                                                                                         >  1:905693/1‑150 (MQ=255)
                                           gcgcAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATg                                                                                                       <  1:1083228/150‑1 (MQ=255)
                                               aaGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAAtt                                                                                                   <  1:1547403/150‑1 (MQ=255)
                                                 ggCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAAtttt                                                                                                 >  2:536032/1‑150 (MQ=255)
                                                 ggCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGAGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAAtttt                                                                                                 <  2:1593256/150‑1 (MQ=255)
                                                  gCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTg                                                                                                <  2:161217/150‑1 (MQ=255)
                                                   cGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAGCGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGa                                                                                               >  1:1015086/1‑150 (MQ=255)
                                                   cGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGa                                                                                               >  1:382821/1‑150 (MQ=255)
                                                    gTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAAt                                                                                                    >  2:1377665/1‑144 (MQ=255)
                                                    gTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAAt                                                                                                    <  1:1377665/144‑1 (MQ=255)
                                                      aGAACATCTCTATGAAGTCGGCACGGGCAAAGTGCTTACTGGCCTGACGAAAAGCATTGTCGACACCCTGACAGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCTCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAgg                                                                                            >  1:1682097/1‑150 (MQ=255)
                                                           aTCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGCACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGaaaaa                                                                                       >  1:993060/1‑150 (MQ=255)
                                                            tctcTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTATAAGAGGAAAATCATGAATTTTGAAGGAAAAAt                                                                                      >  1:774515/1‑150 (MQ=255)
                                                             ctctATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATc                                                                                     <  2:1518050/150‑1 (MQ=255)
                                                                 aTGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACACATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCAc                                                                                 <  1:773648/150‑1 (MQ=255)
                                                                      gTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTa                                                                            >  2:395441/1‑150 (MQ=255)
                                                                         ggCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAAcc                                                                         <  2:628682/150‑1 (MQ=255)
                                                                          gCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCg                                                                        >  2:54589/1‑150 (MQ=255)
                                                                              gggCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGc                                                                    <  2:919310/150‑1 (MQ=255)
                                                                                gCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCGGAACGAACCTTAAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCaa                                                                  >  2:164657/1‑150 (MQ=255)
                                                                                 cAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAg                                                                 <  1:1314409/150‑1 (MQ=255)
                                                                                  aaaGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGc                                                                >  2:14006/1‑150 (MQ=255)
                                                                                  aaaGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGc                                                                <  2:632375/150‑1 (MQ=255)
                                                                                    aGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCcg                                                              <  2:552818/150‑1 (MQ=255)
                                                                                      tGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCcgcg                                                            <  1:1342357/150‑1 (MQ=255)
                                                                                        cTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGa                                                          <  1:961286/150‑1 (MQ=255)
                                                                                          tACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACATTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAAt                                                        >  1:383656/1‑150 (MQ=255)
                                                                                           aCTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAAtt                                                       <  2:1439578/150‑1 (MQ=255)
                                                                                           aCTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAAtt                                                       >  1:1047556/1‑150 (MQ=255)
                                                                                            cTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCATCGGCGCTCGAGCTTTAAAACAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTg                                                      <  2:1060419/150‑1 (MQ=255)
                                                                                             tGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGAGCAAGCCGCGGAATTgg                                                     <  2:1326453/150‑1 (MQ=255)
                                                                                              ggCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGc                                                    <  2:382821/150‑1 (MQ=255)
                                                                                                 cTGACGAAACGCATTGTCGACTCCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcgc                                                 <  1:1595287/150‑1 (MQ=255)
                                                                                                 cTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcgc                                                 >  2:825778/1‑150 (MQ=255)
                                                                                                 cTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcgc                                                 >  2:598495/1‑150 (MQ=255)
                                                                                                 atGACGAAACGCATTGTAGACACACTGACCGCCTCGGCGATGAACGAACCTTCAGCGATTGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcgc                                                 <  2:1682097/149‑1 (MQ=255)
                                                                                                  tGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcgcg                                                <  1:1720172/150‑1 (MQ=255)
                                                                                                  tGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCcgcg                                                <  1:367978/150‑1 (MQ=255)
                                                                                                       aaaCGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAAtt                                           >  1:1522328/1‑150 (MQ=255)
                                                                                                        aaCGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTg                                          <  2:544253/150‑1 (MQ=255)
                                                                                                             aTTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGaa                                     <  1:14006/150‑1 (MQ=255)
                                                                                                                gTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACg                                  >  1:1618419/1‑150 (MQ=255)
                                                                                                                   gACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTc                               >  2:942563/1‑150 (MQ=255)
                                                                                                                       cccTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAg                           >  1:1252825/1‑150 (MQ=255)
                                                                                                                       cccTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAg                           >  1:754145/1‑150 (MQ=255)
                                                                                                                       cccTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAg                           >  2:1005348/1‑150 (MQ=255)
                                                                                                                        ccTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGc                          <  1:1548634/150‑1 (MQ=255)
                                                                                                                        ccTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGc                          >  2:1192236/1‑150 (MQ=255)
                                                                                                                            aCCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGt                      >  1:226926/1‑150 (MQ=255)
                                                                                                                             ccGCCTCGGCGCTGAACGAACCTTCAGCGATGGCTGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTg                     >  1:928820/1‑150 (MQ=255)
                                                                                                                             ccGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTg                     >  1:865443/1‑150 (MQ=255)
                                                                                                                              cGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTgg                    <  1:314323/150‑1 (MQ=255)
                                                                                                                              cGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTgg                    <  1:773309/150‑1 (MQ=255)
                                                                                                                                  tCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCTCTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGcgcg                <  1:704073/150‑1 (MQ=255)
                                                                                                                                  ccatcgcTGAAGGTTCGTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGcgcg                >  1:1405111/5‑150 (MQ=255)
                                                                                                                                     tcgcTGAAGGTTCGTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGaaa             >  1:480576/2‑150 (MQ=255)
                                                                                                                                      cgcTGAAGGTTCGTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAg            >  1:1038977/1‑150 (MQ=255)
                                                                                                                                      cgcTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAg            >  2:526974/1‑150 (MQ=255)
                                                                                                                                      cgcTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAg            >  2:1391957/1‑150 (MQ=255)
                                                                                                                                       gcTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAGt           <  1:942563/150‑1 (MQ=255)
                                                                                                                                           aacgaacCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAGTTAtt       <  1:982222/150‑1 (MQ=255)
                                                                                                                                                acCTTCAGCGAGGGCAGCGGCGCTCGAGCTTTAAAAGATGAAAATCATTAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAGTTATTGGCAc  <  1:183134/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAGTTATTGGCAC  >  NC_000913/1150480‑1150773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: