Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 243 C→T 8.0% N18N (AAC→AAT thrL → thr operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132430CT 8.0% 333.8 / 12.1 112N18N (AAC→AATthrLthr operon leader peptide
Reads supporting (aligned to +/- strand):  ref base C (52/51);  new base T (3/6);  total (55/57)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

Only 100 of 113 total aligned reads displayed.

AGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGC  >  NC_000913/94‑391
                                                                                                                                                     |                                                                                                                                                        
aGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAAc                                                                                                                                                          <  1:469441/150‑1 (MQ=255)
  tAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTCCAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACgg                                                                                                                                                        <  1:1359835/150‑1 (MQ=255)
   aaaTTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGt                                                                                                                                                       <  1:1458839/150‑1 (MQ=255)
   aaaTTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGt                                                                                                                                                       >  2:1346276/1‑150 (MQ=255)
     aTTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGc                                                                                                                                                     <  2:1523711/150‑1 (MQ=255)
      ttAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCg                                                                                                                                                    <  2:456338/150‑1 (MQ=255)
      ttAAAATTTTATTGACTTAGGTAACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACAAAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCg                                                                                                                                                    <  2:325961/150‑1 (MQ=255)
       tAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCgg                                                                                                                                                   >  1:1098441/1‑150 (MQ=255)
       aaagtatttAGTGACCTAAGTCAATAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCgg                                                                                                                                                   >  2:823668/7‑150 (MQ=255)
         aaaTTTTATTGACTTAGGTCACTAAATACTTTACCCAATATAGGCATAGCGCACAGACAGATAAAAATAACAGAGTACACAACATCAAAGAAACGCATTAGCAACACCAATACCACCACCATAACAATTACCAAAGGTAACGGTGAGGGc                                                                                                                                                 >  1:93108/1‑150 (MQ=255)
         aaaTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGTgatggt                                                                                                                                                 <  1:472417/150‑6 (MQ=255)
         aaaTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGTgatggt                                                                                                                                                 <  1:151751/150‑6 (MQ=255)
          aaTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGTgatggtg                                                                                                                                                <  2:1154501/150‑7 (MQ=255)
           aTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGTgatggtgg                                                                                                                                               <  1:1438047/150‑8 (MQ=255)
           aTTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCGTTACCTGTGGTAATGGTgatggtgg                                                                                                                                               <  1:1389996/150‑8 (MQ=255)
              ttATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGAcg                                                                                                                                            <  2:352459/150‑1 (MQ=255)
                   gACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTAc                                                                                                                                       >  1:435786/1‑150 (MQ=255)
                    aCTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGAAACGGTGCGGGCTGACGCGTACa                                                                                                                                      <  2:386667/150‑1 (MQ=255)
                          gTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAac                                                                                                                                <  1:1126133/150‑1 (MQ=255)
                          gTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAac                                                                                                                                >  2:178938/1‑150 (MQ=255)
                          gTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAac                                                                                                                                >  1:1662501/1‑150 (MQ=255)
                             aCTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAAcaca                                                                                                                             >  2:794818/1‑150 (MQ=255)
                               tAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGa                                                                                                                          <  1:1013869/150‑1 (MQ=255)
                                aaaTACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGaa                                                                                                                         >  2:1359183/1‑150 (MQ=255)
                                    aCTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGaaaaaa                                                                                                                     >  2:1467998/1‑150 (MQ=255)
                                    aCTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGaaaaaa                                                                                                                     >  1:1178123/1‑150 (MQ=255)
                                     cTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAg                                                                                                                    <  2:1178123/150‑1 (MQ=255)
                                       ttAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGcc                                                                                                                  <  1:823668/150‑1 (MQ=255)
                                          aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                               >  2:1248978/1‑150 (MQ=255)
                                          aCCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGc                                                                                                               <  2:1063421/150‑1 (MQ=255)
                                            cAATATAGGCATAGCGCCCAGACAGATAAAAAATACCGAGTACACAACATCCATGAAACGAAATACCACCAACATTACCACAACCATCACCATTTCCACAGGGAACGGTGCGGCATGACGCGAACAGGAAGCACAGAACAAAACCCCCAc                                                                                                             >  1:1142372/1‑150 (MQ=255)
                                                 taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGAc                                                                                                        >  1:993145/1‑150 (MQ=255)
                                                    gCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGt                                                                                                    >  1:180572/1‑150 (MQ=255)
                                                       tAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCg                                                                                                 >  2:864179/1‑150 (MQ=255)
                                                        aGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCgg                                                                                                <  1:335805/150‑1 (MQ=255)
                                                         gcgcACAGACAGATAAAAATTACAGAGTACACAACATCGATGAAACGCATAAGCACCACCATTACCACCACCATCACCATTACCACAGGTATCGCGGGGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCggg                                                                                               <  2:1406653/150‑1 (MQ=255)
                                                         gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCggg                                                                                               <  2:1638288/150‑1 (MQ=255)
                                                          cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGc                                                                                              <  1:1467998/150‑1 (MQ=255)
                                                          cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGc                                                                                              <  2:1098441/150‑1 (MQ=255)
                                                           gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCt                                                                                            >  1:1422712/1‑150 (MQ=255)
                                                           gcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCt                                                                                            <  1:1617580/150‑1 (MQ=255)
                                                            cacaGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtt                                                                                           <  2:1037534/150‑1 (MQ=255)
                                                             acagacagATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCttt                                                                                          <  1:705800/150‑1 (MQ=255)
                                                                gacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCCGACAGTGCGGGCtttttt                                                                                      >  1:1468242/1‑150 (MQ=255)
                                                                  cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt                                                                                    >  2:1665723/1‑150 (MQ=255)
                                                                  cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCGGACAGTGCGGGCtttttttt                                                                                    >  1:186766/1‑150 (MQ=255)
                                                                  attaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCttttttct                                                                                    <  2:1662501/147‑3 (MQ=39)
                                                                   agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttttt                                                                                   <  2:1183107/150‑1 (MQ=255)
                                                                   agaTAAAAATTACAGAGGACTCAACATCCATGAACCGCATTAGCACCACCATAACCACCACCATCACCATAACCACAGGTAACGGTGCGGGCTGAAGCGTACAGGAAACACAGAAAAAAAGCCCGCACTGTCAGGTGCGGGCttttttct                                                                                    <  2:278534/150‑3 (MQ=37)
                                                                    gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTc                                                                                  >  2:875165/1‑150 (MQ=255)
                                                                     aTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACTGTCAGGTGCGGGCttttttctgtg                                                                                 <  2:149740/150‑6 (MQ=39)
                                                                      tAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCgc                                                                                >  1:941855/1‑149 (MQ=255)
                                                                       aaaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGATACACCGAAAAAAGCCCGCACTGTCAGGTGCGGGCttttttctgtgtt                                                                               <  1:271591/150‑8 (MQ=38)
                                                                        aaaaTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATAACCACAGGTAACGGTGCGGGCTGACGGGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGAGCGGGCTTTTTTTTTCGagc                                                                              >  2:837152/1‑148 (MQ=255)
                                                                               cAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTa                                                                       <  1:1462508/150‑1 (MQ=255)
                                                                               cAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTa                                                                       <  1:1346276/150‑1 (MQ=255)
                                                                                    tACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCAACACCATTACCACAGGTAACGGTGCGGGCTGACGCGAACAGGAAACACAGAAAAAAGCCCGCACATGACAGTGCGGGCTTTTTTTTTCGACCAAAGATAACCAg                                                                  >  1:88768/1‑150 (MQ=255)
                                                                                      cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGt                                                                >  1:1481330/1‑150 (MQ=255)
                                                                                           caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTaacaa                                                           >  1:790084/1‑150 (MQ=255)
                                                                                           caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGGAACGAGGTaacaa                                                           >  2:852408/1‑150 (MQ=255)
                                                                                             aacATTAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACATAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAAcc                                                         <  2:1439670/148‑1 (MQ=255)
                                                                                              ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa                                                        <  2:707146/150‑1 (MQ=255)
                                                                                              ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa                                                        <  2:701122/150‑1 (MQ=255)
                                                                                                 tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCCACCAAAGGTACCGAGGTAACAACCATGc                                                     >  1:313501/1‑150 (MQ=255)
                                                                                                    aaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAg                                                  <  1:178938/150‑1 (MQ=255)
                                                                                                      cGCATTAGCACCACCATTACCACCACCATCACCATTACCTCAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACTGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgtg                                                <  2:1408212/150‑1 (MQ=255)
                                                                                                           tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa                                           <  1:1665723/150‑1 (MQ=255)
                                                                                                            aGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAg                                          >  1:789747/1‑150 (MQ=255)
                                                                                                             gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGt                                         >  2:1501786/1‑150 (MQ=255)
                                                                                                               accaccATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc                                       >  2:1525902/1‑150 (MQ=255)
                                                                                                                ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg                                      >  2:1192090/1‑150 (MQ=255)
                                                                                                                caaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGATACACTGAAAAAAGGCGGCACCTGACAGTGCGGGCTTTTTTTTTCGACCATAGGTAACGAGGTAACAAGCATGCGAGTGATGAAGTTcg                                      <  2:939580/148‑1 (MQ=255)
                                                                                                                 caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg                                     <  1:1680191/150‑1 (MQ=255)
                                                                                                                   ccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACCACCATGCGAGTGGTGAAGTTcggcg                                   >  2:1205127/1‑150 (MQ=255)
                                                                                                                      ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa                                <  1:1359/150‑1 (MQ=255)
                                                                                                                      ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa                                <  2:568944/150‑1 (MQ=255)
                                                                                                                       tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAc                               <  2:1422712/150‑1 (MQ=255)
                                                                                                                       tACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTAc                               <  1:794818/150‑1 (MQ=255)
                                                                                                                         ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt                             >  1:870123/1‑150 (MQ=255)
                                                                                                                         caaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCACGCACCTGACAGTGGGGGCTGTTTTTTTCGACCAAAGGTAAAGAGGTAACACCACTGCGAGTTTTGAAGTTAGGCGGTACAt                             >  2:1280236/3‑150 (MQ=255)
                                                                                                                          caccaccaTAACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACAAAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATc                            <  2:88768/150‑1 (MQ=255)
                                                                                                                            ccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAg                          >  1:931054/1‑150 (MQ=255)
                                                                                                                                ccgcaccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTATATTTCGACCAAAGGTAACGATGTAACAACCATGCGAGTGTTGAAGTTAGGCGGTACAACAGTGGc                      >  2:589140/4‑150 (MQ=255)
                                                                                                                                catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc                      >  1:1573245/1‑150 (MQ=255)
                                                                                                                                   caccgtTACCTGTGGTAATGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTGTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCaa                    >  1:1109522/1‑150 (MQ=255)
                                                                                                                                    accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCGTTTTTTTTCGCCCAAAGGTAACGAGGTTACAACCATGCGAGTGTTTAAGTACGGCGGTACATCAGTGGCAAAt                  >  1:1528644/1‑150 (MQ=255)
                                                                                                                                      caatACAACAGGTAACGGTCCGGGCTGACGGGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCATTGGCAAATg                 <  2:878638/147‑1 (MQ=255)
                                                                                                                                       atTACCACAGGTAACGGTGCGGGCTGACGGGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa               <  1:1609719/150‑1 (MQ=255)
                                                                                                                                          aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa            >  1:762714/1‑150 (MQ=255)
                                                                                                                                          aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAAAAAACATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa            >  1:562923/1‑150 (MQ=255)
                                                                                                                                          aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGAAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATTAAGaa            >  1:762716/1‑150 (MQ=255)
                                                                                                                                          aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTGTTTTTTTCGACCAAAGGTAACGAGTGTACAACCATGCGAGTGTTGAAGTTGGGGGGTACATAAGTGGCAAATGCAGaa            >  2:1089475/1‑150 (MQ=255)
                                                                                                                                           ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAAc           >  1:1507716/1‑150 (MQ=255)
                                                                                                                                             acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATACAGAACGt         <  2:1483218/150‑1 (MQ=255)
                                                                                                                                             acaGGTAACGGTGCGGGATGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTTACAACCATGCGAGTGTTTAAGTTGGGCGGTACATAAGTGCCAAAAGCAGAACGt         >  1:756242/1‑150 (MQ=255)
                                                                                                                                                ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGtttt      >  1:1459414/1‑150 (MQ=255)
                                                                                                                                                 gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc     <  2:146419/150‑1 (MQ=255)
                                                                                                                                                   aaCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTg   >  2:578839/1‑150 (MQ=255)
                                                                                                                                                   aaCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTg   >  1:1424896/1‑150 (MQ=255)
                                                                                                                                                    aCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGc  >  1:495280/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
AGTAAATTAAAATTTTATTGACTTAGGTCACTAAATACTTTAACCAATATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGC  >  NC_000913/94‑391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: