Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,157,633 A→T 5.5% intergenic (+59/‑236) ycfH → / → ptsG putative DNase/fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,157,6330AT 5.5% 326.0 / 6.2 109intergenic (+59/‑236)ycfH/ptsGputative DNase/fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (55/48);  new base T (3/3);  total (58/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

Only 100 of 110 total aligned reads displayed.

TGCCGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTT  >  NC_000913/1157487‑1157782
                                                                                                                                                  |                                                                                                                                                     
tGCCGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCAGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTg                                                                                                                                                    <  1:1133365/150‑1 (MQ=255)
 gCCGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTgg                                                                                                                                                   >  2:846536/1‑150 (MQ=255)
  ccGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTggg                                                                                                                                                  <  1:1463824/150‑1 (MQ=255)
   cGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGc                                                                                                                                                 >  2:1152091/1‑150 (MQ=255)
   cGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGc                                                                                                                                                 <  2:1297733/150‑1 (MQ=255)
    gTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCg                                                                                                                                                <  1:1250341/150‑1 (MQ=255)
    gTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGACCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCAGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCg                                                                                                                                                >  2:803391/1‑150 (MQ=255)
       gaagaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTg                                                                                                                                             >  2:72588/1‑150 (MQ=255)
        aagaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGa                                                                                                                                            >  2:1425936/1‑150 (MQ=255)
         agaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGaa                                                                                                                                           >  1:1283774/1‑150 (MQ=255)
          gaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGTGaac                                                                                                                                          <  1:1256536/150‑2 (MQ=255)
          gaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGTGaac                                                                                                                                          <  1:1257395/150‑2 (MQ=255)
          gaaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCAGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTAGTAATTAATGGCTAAAACGAGTAAAGTTCACCTCCGAAAATTGGGCGGTGAAt                                                                                                                                          >  2:883891/1‑150 (MQ=255)
           aaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATa                                                                                                                                         >  1:887461/1‑150 (MQ=255)
           aaCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATa                                                                                                                                         <  1:1152091/150‑1 (MQ=255)
            aCTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCCAATTTTCGGCGGTGaactt                                                                                                                                        <  1:1712779/150‑4 (MQ=255)
             cTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAAc                                                                                                                                       >  2:468201/1‑150 (MQ=255)
             cTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAAc                                                                                                                                       <  1:600459/150‑1 (MQ=255)
                 cgcAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATACCCACg                                                                                                                                   >  1:534016/1‑150 (MQ=255)
                 cgcAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACg                                                                                                                                   >  2:784014/1‑150 (MQ=255)
                 cgcAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACg                                                                                                                                   >  2:408223/1‑150 (MQ=255)
                 cgcAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACg                                                                                                                                   >  1:509629/1‑150 (MQ=255)
                  gcAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGt                                                                                                                                  <  1:816720/150‑1 (MQ=255)
                     ggTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTg                                                                                                                               >  1:769208/1‑150 (MQ=255)
                     ggTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTg                                                                                                                               >  1:142386/1‑150 (MQ=255)
                      gTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGa                                                                                                                              <  1:1161687/150‑1 (MQ=255)
                       tAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGaa                                                                                                                             >  1:355610/1‑150 (MQ=255)
                         accaccGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAat                                                                                                                           >  1:1133402/1‑150 (MQ=255)
                         accaccGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCAGTTGAATTAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAat                                                                                                                           <  2:123587/150‑1 (MQ=255)
                          ccaccGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAata                                                                                                                          >  1:481612/1‑150 (MQ=255)
                                   aCTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACat                                                                                                                 >  1:461577/1‑150 (MQ=255)
                                     ttCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACatat                                                                                                               >  1:259853/1‑150 (MQ=255)
                                       cGCCCGTCTGTTTCACATCGACGCATCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCAAGTTTGAAATATTGTGACatattt                                                                                                             >  1:1087876/1‑148 (MQ=255)
                                        gCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGtt                                                                                                            <  2:481612/150‑1 (MQ=255)
                                         cccGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGttt                                                                                                           <  2:885259/150‑1 (MQ=255)
                                            gTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGt                                                                                                        <  2:142386/150‑1 (MQ=255)
                                              cTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCa                                                                                                      <  2:410725/150‑1 (MQ=255)
                                              cTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCa                                                                                                      <  1:72588/150‑1 (MQ=255)
                                               tGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCaa                                                                                                     <  2:355610/150‑1 (MQ=255)
                                                gTTTCACATCGACGCTTCCCGCCTTCAATCAATCCGTAGAATGAGTTTTTTTAAACCTCGTAATTAATGGCTAAAACGAGTAAAGATCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAAAAATTTGAAATAAGTTTTGTCaaa                                                                                                    >  1:548759/1‑150 (MQ=255)
                                                  ttCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAt                                                                                                  >  1:372880/1‑150 (MQ=255)
                                                  ttCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAt                                                                                                  <  2:1412702/150‑1 (MQ=255)
                                                   tCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAAtg                                                                                                 <  2:1133402/150‑1 (MQ=255)
                                                      caTCGACGCTTCCCGCCTTCAATCCCTCCGTTGACTGAGTTTTGTTACAGCTCGTAATTAATGGCTAAACCGAGTAAAGTTCACAGCCGAAAAGTGGGCGGTGAATAAACACGTTTGAAATATTGTGACCTATGTTTTGTCAAAATGTGc                                                                                              >  1:71079/1‑150 (MQ=255)
                                                       aTCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCa                                                                                             >  1:910945/1‑150 (MQ=255)
                                                       aTCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCa                                                                                             <  2:259853/150‑1 (MQ=255)
                                                         cGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAAc                                                                                           <  2:1358907/150‑1 (MQ=255)
                                                           aCGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGGAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATTTGCAACtt                                                                                         >  1:860806/1‑150 (MQ=255)
                                                              cTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACAGCCGAAAATTCGGCTGTGAATACACTCGTTTGAATTATTGTGACATATGTTTTGTCAAAATGTGCAACTtctc                                                                                      >  1:252997/1‑150 (MQ=255)
                                                                   cGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGTCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATg                                                                                 <  1:105844/150‑1 (MQ=255)
                                                                     ccTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCTCGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGAt                                                                               >  1:1403247/1‑150 (MQ=255)
                                                                     ccTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGAt                                                                               >  1:1342923/1‑150 (MQ=255)
                                                                      cTTCAATCTATAATTTTAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACTAGTAAAGTTCACCGAAGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATc                                                                              <  1:1543051/150‑1 (MQ=255)
                                                                       ttCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTTGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCACCAATGATCt                                                                             >  1:139991/1‑150 (MQ=255)
                                                                       ttCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCt                                                                             <  1:1129418/150‑1 (MQ=255)
                                                                       ttCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCt                                                                             <  2:769208/150‑1 (MQ=255)
                                                                           atccatccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAg                                                                         <  1:795545/150‑1 (MQ=255)
                                                                           atccatccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAg                                                                         <  1:1088650/150‑1 (MQ=255)
                                                                            tccatccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGt                                                                        <  1:846536/150‑1 (MQ=255)
                                                                             ccatccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGtt                                                                       <  1:1264732/150‑1 (MQ=255)
                                                                              catccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTg                                                                      >  2:1673337/1‑150 (MQ=255)
                                                                              catccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTg                                                                      <  2:521475/150‑1 (MQ=255)
                                                                              catccGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTg                                                                      <  2:517293/150‑1 (MQ=255)
                                                                                  cGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAAc                                                                  >  1:1524597/1‑150 (MQ=255)
                                                                                   gTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACg                                                                 >  2:1057358/1‑150 (MQ=255)
                                                                                   gTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACg                                                                 >  2:76049/1‑150 (MQ=255)
                                                                                   gTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACg                                                                 <  1:468201/150‑1 (MQ=255)
                                                                                      gAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGa                                                              >  2:930031/1‑150 (MQ=255)
                                                                                      gAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGa                                                              >  1:1529255/1‑150 (MQ=255)
                                                                                       aaTGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGaa                                                             >  1:945161/1‑149 (MQ=255)
                                                                                           aGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGcc                                                         >  1:967462/1‑150 (MQ=255)
                                                                                            gTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCg                                                        >  2:1206158/1‑150 (MQ=255)
                                                                                              ttttttAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTc                                                      >  1:1666812/1‑150 (MQ=255)
                                                                                               tttttAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTca                                                     >  2:668286/1‑150 (MQ=255)
                                                                                                ttttAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCAAATGATCTGAAGTTGAAACGTGATAGCCGTaaa                                                    >  2:190485/1‑150 (MQ=255)
                                                                                                   tAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTcaaaca                                                 <  1:1206158/150‑1 (MQ=255)
                                                                                                   tAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATCTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTcaaaca                                                 >  1:1418705/1‑150 (MQ=255)
                                                                                                    aaaGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTcaaacaa                                                >  1:1528056/1‑150 (MQ=255)
                                                                                                      aGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAAt                                              >  2:380655/1‑150 (MQ=255)
                                                                                                       gCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCGGAAGTTGAAACGTGATAGCCGTCAAACAAAtt                                             >  2:633922/1‑150 (MQ=255)
                                                                                                          cGTAATTAATGTCTAAAACGAGTAAAGTTCACCGGCGAAAATTGGGCGGTGAATAACCACGTTTGAATTATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGc                                          <  1:347413/150‑1 (MQ=255)
                                                                                                             aattaatGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGAGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACt                                       <  2:71079/150‑1 (MQ=255)
                                                                                                              attaatGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTg                                      <  2:461577/150‑1 (MQ=255)
                                                                                                                 aatGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAat                                   <  2:372880/150‑1 (MQ=255)
                                                                                                                   tGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAattt                                 >  1:1316247/1‑149 (MQ=255)
                                                                                                                    ggCCAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAattat                                <  1:1487262/150‑1 (MQ=255)
                                                                                                                      cTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTAttt                              <  2:317159/150‑1 (MQ=255)
                                                                                                                         aaaCGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTAc                           <  2:1409543/150‑1 (MQ=255)
                                                                                                                             gAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCtg                       >  1:572800/1‑150 (MQ=255)
                                                                                                                               gTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCtgtg                     >  1:17932/1‑150 (MQ=255)
                                                                                                                                tAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTgtgt                    <  2:50127/150‑1 (MQ=255)
                                                                                                                                tAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTgtgt                    <  1:1708168/150‑1 (MQ=255)
                                                                                                                                     ttCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaataa               >  2:775783/1‑150 (MQ=255)
                                                                                                                                     ttCACCGCCCAATTTTCGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaataa               >  1:1455976/1‑150 (MQ=255)
                                                                                                                                     ttCACCGCCCAATTTTCGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGtaataa               >  1:1161592/1‑150 (MQ=255)
                                                                                                                                      tCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaa              >  1:1653797/1‑150 (MQ=255)
                                                                                                                                      tCACCGCCCAATTTTCGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTaataaa              >  2:1554129/1‑150 (MQ=255)
                                                                                                                                           gccgAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGATACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAAtaaataaat         >  1:1460074/1‑149 (MQ=255)
                                                                                                                                             cgAAAATTGGTCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAggg       <  1:1048208/150‑1 (MQ=255)
                                                                                                                                                  aTTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAAAAAATAAAGGGCGCtt  <  2:583228/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TGCCGTTGAAGAACTGGCGCAGGTAACCACCGATAACTTCGCCCGTCTGTTTCACATCGACGCTTCCCGCCTTCAATCCATCCGTTGAATGAGTTTTTTTAAAGCTCGTAATTAATGGCTAAAACGAGTAAAGTTCACCGCCGAAAATTGGGCGGTGAATAACCACGTTTGAAATATTGTGACATATGTTTTGTCAAAATGTGCAACTTCTCCAATGATCTGAAGTTGAAACGTGATAGCCGTCAAACAAATTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCGCTT  >  NC_000913/1157487‑1157782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: