Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,175,524 G→C 5.1% G33A (GGC→GCC)  lolC → lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,175,5240GC 5.1% 379.3 / 9.2 118G33A (GGC→GCC) lolClipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base G (58/54);  new base C (2/4);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.72e-01

Only 100 of 121 total aligned reads displayed.

CTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAA  >  NC_000913/1175375‑1175672
                                                                                                                                                     |                                                                                                                                                    
catGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCgg                                                                                                                                                      >  1:1571793/3‑150 (MQ=255)
cTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCgg                                                                                                                                                      <  2:676847/150‑1 (MQ=255)
    cTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCTTGCAGCGGATCGCTTAGGTCGTTTAGTCTCCTGGCTTTATAACATCTGCAtt                                                                                                                                                  >  1:1286928/1‑150 (MQ=255)
    cTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCAtt                                                                                                                                                  >  1:247504/1‑150 (MQ=255)
      ttCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTAc                                                                                                                                                <  2:953962/150‑1 (MQ=255)
        cGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTAccc                                                                                                                                              <  2:979086/150‑1 (MQ=255)
           ttATATACTCGAGTCTTTTCTACAGCAACCAGACGGATTTCTTGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTTGCTTTCTACCATCGGCTTTACCCTCg                                                                                                                                           <  1:747607/150‑1 (MQ=255)
            tatataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCgg                                                                                                                                          >  1:748307/1‑150 (MQ=255)
            tatataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGAGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCgg                                                                                                                                          >  2:1401377/1‑150 (MQ=255)
              tataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGAGCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCgggg                                                                                                                                        <  1:1131399/150‑1 (MQ=255)
               ataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGCCATCAccccgagggt                                                                                                                                       <  1:1427213/150‑8 (MQ=255)
                taCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTg                                                                                                                                      >  1:1654933/1‑150 (MQ=255)
                  cTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGAt                                                                                                                                    >  1:455741/1‑150 (MQ=255)
                    cGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATgg                                                                                                                                  >  1:1015167/1‑150 (MQ=255)
                    cGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGATTCGTCTACTGGCTTTCTACCATCGGCATTACCCACGGGCTGATgc                                                                                                                                  >  2:699869/1‑149 (MQ=255)
                      tgtCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGgcg                                                                                                                                >  2:12123/1‑150 (MQ=255)
                         cTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTg                                                                                                                             >  2:1052249/1‑150 (MQ=255)
                         cTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTg                                                                                                                             <  2:1437466/150‑1 (MQ=255)
                           ttGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGt                                                                                                                           <  2:1506778/150‑1 (MQ=255)
                           ttGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGCCATCACCCCGAGGGTAATGCCGATGGt                                                                                                                           <  2:1204355/150‑1 (MQ=255)
                              cTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTcac                                                                                                                        >  2:200672/1‑150 (MQ=255)
                                aCAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAg                                                                                                                      <  2:1512823/150‑1 (MQ=255)
                                aCAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAg                                                                                                                      >  1:22506/1‑150 (MQ=255)
                                 aaGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCAGCGCCATCACCCCGAGGGTAATGCCGATGGtagaaag                                                                                                                     <  2:1413531/149‑7 (MQ=37)
                                      aCCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCAGGTCACAGTATTGt                                                                                                                >  2:1280279/1‑150 (MQ=255)
                                         aGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAg                                                                                                             <  1:200672/150‑1 (MQ=255)
                                           aCGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGGGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtg                                                                                                           <  1:95171/150‑1 (MQ=255)
                                            cGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtga                                                                                                          >  1:1029012/1‑150 (MQ=255)
                                            cGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGGCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtga                                                                                                          <  1:440167/150‑1 (MQ=255)
                                                  acATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGCGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACg                                                                                                    <  1:256613/149‑1 (MQ=255)
                                                     tGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCt                                                                                                 >  1:1305118/1‑150 (MQ=255)
                                                      gTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCtt                                                                                                >  2:1411179/1‑150 (MQ=255)
                                                      gTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGCGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCtt                                                                                                <  1:1401377/150‑1 (MQ=255)
                                                       tACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCttt                                                                                               >  1:468040/1‑150 (MQ=255)
                                                        aCCCACCTGTCGCTCTATATATTGGCCTGCGCTACATGCGTGGGCGGGCAGCGGAGCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                              <  1:118420/150‑1 (MQ=255)
                                                        aCCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                              >  2:1279019/1‑150 (MQ=255)
                                                        aCCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                              >  1:1006014/1‑150 (MQ=255)
                                                        aCCAACCTGACGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                              <  2:1654933/150‑1 (MQ=255)
                                                          cAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCGCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTgcg                                                                                            >  2:1096882/1‑148 (MQ=255)
                                                           aaCCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAgc                                                                                           >  1:851015/1‑150 (MQ=255)
                                                           aaCCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGGCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAgc                                                                                           <  2:559245/150‑1 (MQ=255)
                                                               tGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGa                                                                                       >  1:1367045/1‑150 (MQ=255)
                                                               tGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGa                                                                                       >  2:300287/1‑150 (MQ=255)
                                                                gTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGAAGGCGCTGGTCACAGTATTGTCAGAGATGAACGGCTTTGAGCGCGAg                                                                                      >  2:326269/1‑150 (MQ=255)
                                                                 tCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGc                                                                                     <  1:985486/150‑1 (MQ=255)
                                                                 ccGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGAGGAACGGCTTTGAGCGCGAGc                                                                                     <  2:1303416/149‑1 (MQ=255)
                                                                    ctctATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCAAAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGc                                                                                  >  2:260666/1‑150 (MQ=255)
                                                                     tctATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCa                                                                                 >  1:722650/1‑150 (MQ=255)
                                                                      ctATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCaa                                                                                >  2:833918/1‑150 (MQ=255)
                                                                       tatttcttGGCCTGCGTTACATGCGTGCGCTTGCAGCGGATCGCTTCGGTCGTCTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCaaa                                                                               <  1:837059/150‑1 (MQ=255)
                                                                             ttGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAacaaca                                                                         >  1:1147197/1‑150 (MQ=255)
                                                                             ttGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCGGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAacaaca                                                                         >  1:1499713/1‑150 (MQ=255)
                                                                               ggCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACACTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATc                                                                       >  2:1073345/1‑150 (MQ=255)
                                                                                gCCTGCGTTACATGCGTGGGCTTGCAGCGGATCGCTTCGGACGTTTCGTCTCCTGGCTTTCTACCATCGGCAATACCCTCGGCTTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACATCATcc                                                                      >  2:1711143/1‑150 (MQ=255)
                                                                                gCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATcc                                                                      >  1:1467253/1‑150 (MQ=255)
                                                                                 ccTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCt                                                                     <  1:501237/150‑1 (MQ=255)
                                                                                   tGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTg                                                                   >  1:1477202/1‑150 (MQ=255)
                                                                                     cGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGc                                                                 <  1:506690/150‑1 (MQ=255)
                                                                                      gTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGcc                                                                <  2:22506/150‑1 (MQ=255)
                                                                                       ttACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCt                                                               >  1:1195127/1‑150 (MQ=255)
                                                                                        tACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTg                                                              <  2:1367045/150‑1 (MQ=255)
                                                                                         aCATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGa                                                             >  2:400024/1‑150 (MQ=255)
                                                                                           aTGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATg                                                           <  2:691148/150‑1 (MQ=255)
                                                                                            tGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACACCATCCTAGGCCTGATGc                                                          >  2:111605/1‑150 (MQ=255)
                                                                                            tGCGTGGGCGTGCAGCGGATCGCTTCGGGCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTAGTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGc                                                          <  1:279850/150‑1 (MQ=255)
                                                                                             gCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGcc                                                         <  1:1052249/150‑1 (MQ=255)
                                                                                                  ggCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAgg                                                    <  2:1305118/150‑1 (MQ=255)
                                                                                                  ggCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAgg                                                    <  1:12123/150‑1 (MQ=255)
                                                                                                      tGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAAt                                                <  2:748307/150‑1 (MQ=255)
                                                                                                      tGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGACGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAAt                                                >  2:604679/1‑150 (MQ=255)
                                                                                                       gCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAAtt                                               >  1:1699790/1‑150 (MQ=255)
                                                                                                        cAGCGGATCGCTTCGGTCGATTAGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGAATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCATTGGCCAGATGCCACAGGCAATtc                                              >  2:350508/1‑150 (MQ=255)
                                                                                                                  cTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTgc                                    >  1:845731/1‑149 (MQ=255)
                                                                                                                  cTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGa                                    >  1:1130102/1‑150 (MQ=255)
                                                                                                                  cTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGa                                    <  2:349905/150‑1 (MQ=255)
                                                                                                                    tCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGACGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGc                                  <  1:604679/150‑1 (MQ=255)
                                                                                                                      ggTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCAt                                >  2:317439/1‑150 (MQ=255)
                                                                                                                          gTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGc                                                   <  2:417198/127‑1 (MQ=255)
                                                                                                                          gTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGc                                                   >  1:417198/1‑127 (MQ=255)
                                                                                                                            ttCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGctct                          <  2:247504/150‑1 (MQ=255)
                                                                                                                              cGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGctctct                        >  1:423286/1‑150 (MQ=255)
                                                                                                                                   ccTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAAcc                   <  1:400024/150‑1 (MQ=255)
                                                                                                                                    cTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAAccc                  <  1:342686/150‑1 (MQ=255)
                                                                                                                                    cTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAAccc                  >  1:888087/1‑150 (MQ=255)
                                                                                                                                      ggCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgc                >  1:1064499/1‑150 (MQ=255)
                                                                                                                                       gCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgca               >  1:930288/1‑150 (MQ=255)
                                                                                                                                        cTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcag              <  2:403085/150‑1 (MQ=255)
                                                                                                                                        cTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcag              <  1:1110123/150‑1 (MQ=255)
                                                                                                                                        cTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcag              <  2:851015/150‑1 (MQ=255)
                                                                                                                                         tttCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTTAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc             <  2:1493307/150‑1 (MQ=255)
                                                                                                                                         tttCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc             <  2:1499713/150‑1 (MQ=255)
                                                                                                                                         tttCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc             <  2:1147197/150‑1 (MQ=255)
                                                                                                                                         tttCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc             <  2:324988/150‑1 (MQ=255)
                                                                                                                                            cTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAAc          <  2:1015167/150‑1 (MQ=255)
                                                                                                                                             tACCAGCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCGGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACt         <  1:111605/150‑1 (MQ=255)
                                                                                                                                               ccATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTcc       <  2:682359/150‑1 (MQ=255)
                                                                                                                                               ccATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTcc       <  2:567081/150‑1 (MQ=255)
                                                                                                                                               ccATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTcc       <  2:560297/150‑1 (MQ=255)
                                                                                                                                                cATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTccc      <  2:455741/150‑1 (MQ=255)
                                                                                                                                                 aTCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCa     >  2:1163832/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTTGCTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAA  >  NC_000913/1175375‑1175672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: