Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,175,528 T→C 5.1% I34I (ATT→ATC lolC → lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,175,5280TC 5.1% 358.4 / 6.8 118I34I (ATT→ATClolClipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (58/54);  new base C (2/4);  total (60/58)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-01

Only 100 of 120 total aligned reads displayed.

CTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAAC  >  NC_000913/1175379‑1175674
                                                                                                                                                     |                                                                                                                                                  
cTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCTTGCAGCGGATCGCTTAGGTCGTTTAGTCTCCTGGCTTTATAACATCTGCAtt                                                                                                                                                    >  1:1286928/1‑150 (MQ=255)
cTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCAtt                                                                                                                                                    >  1:247504/1‑150 (MQ=255)
  ttCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTAc                                                                                                                                                  <  2:953962/150‑1 (MQ=255)
    cGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTAccc                                                                                                                                                <  2:979086/150‑1 (MQ=255)
        tatataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCgg                                                                                                                                            >  1:748307/1‑150 (MQ=255)
        tatataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCgg                                                                                                                                            >  1:443161/1‑150 (MQ=255)
        tatataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGAGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCgg                                                                                                                                            >  2:1401377/1‑150 (MQ=255)
          tataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGAGCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCgggg                                                                                                                                          <  1:1131399/150‑1 (MQ=255)
           ataCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGCCATCAccccgagggt                                                                                                                                         <  1:1427213/150‑8 (MQ=255)
            taCTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTg                                                                                                                                        >  1:1654933/1‑150 (MQ=255)
              cTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGAt                                                                                                                                      >  1:455741/1‑150 (MQ=255)
                cGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATgg                                                                                                                                    >  1:1015167/1‑150 (MQ=255)
                cGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGATTCGTCTACTGGCTTTCTACCATCGGCATTACCCACGGGCTGATgc                                                                                                                                    >  2:699869/1‑149 (MQ=255)
                  tgtCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGgcg                                                                                                                                  >  2:12123/1‑150 (MQ=255)
                     cTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTg                                                                                                                               <  2:1437466/150‑1 (MQ=255)
                       ttGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGt                                                                                                                             <  2:1506778/150‑1 (MQ=255)
                       ttGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGCCATCACCCCGAGGGTAATGCCGATGGt                                                                                                                             <  2:1204355/150‑1 (MQ=255)
                        tGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTc                                                                                                                            <  2:1105723/150‑1 (MQ=255)
                          cTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTcac                                                                                                                          >  2:200672/1‑150 (MQ=255)
                           tACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCAGCGCCATCACCCCGAGGGTAATGCCGATGGtagaa                                                                                                                         <  2:620089/150‑5 (MQ=38)
                                  aCCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCAGGTCACAGTATTGt                                                                                                                  >  2:1280279/1‑150 (MQ=255)
                                     aGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAg                                                                                                               <  1:200672/150‑1 (MQ=255)
                                      gACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGt                                                                                                              >  1:324988/1‑150 (MQ=255)
                                       aCGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGGGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtg                                                                                                             <  1:95171/150‑1 (MQ=255)
                                        cGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtga                                                                                                            >  1:1029012/1‑150 (MQ=255)
                                        cGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGGCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtga                                                                                                            <  1:440167/150‑1 (MQ=255)
                                          gATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTAACCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtgatt                                                                                                          >  2:1256672/1‑149 (MQ=255)
                                           aTTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCTGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGtgatga                                                                                                         >  2:299374/1‑150 (MQ=255)
                                              acATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGCGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACg                                                                                                      <  1:256613/149‑1 (MQ=255)
                                                 tGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCt                                                                                                   >  1:1305118/1‑150 (MQ=255)
                                                  gTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCtt                                                                                                  >  2:1411179/1‑150 (MQ=255)
                                                  gTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGCGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCtt                                                                                                  <  1:1401377/150‑1 (MQ=255)
                                                    aCCCACCTGTCGCTCTATATATTGGCCTGCGCTACATGCGTGGGCGGGCAGCGGAGCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                                <  1:118420/150‑1 (MQ=255)
                                                    aCCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                                <  2:1353860/150‑1 (MQ=255)
                                                    aCCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                                >  2:1279019/1‑150 (MQ=255)
                                                    aCCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                                >  1:1006014/1‑150 (MQ=255)
                                                    aCCAACCTGACGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTg                                                                                                <  2:1654933/150‑1 (MQ=255)
                                                      cAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCGCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTgcg                                                                                              >  2:1096882/1‑148 (MQ=255)
                                                       aaCCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAgc                                                                                             >  1:851015/1‑150 (MQ=255)
                                                       aaCCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGGCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAgc                                                                                             <  2:559245/150‑1 (MQ=255)
                                                          cTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGcgcg                                                                                          >  2:342686/1‑150 (MQ=255)
                                                           tGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGa                                                                                         >  1:1367045/1‑150 (MQ=255)
                                                           tGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGa                                                                                         >  2:300287/1‑150 (MQ=255)
                                                             tCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGc                                                                                       <  1:985486/150‑1 (MQ=255)
                                                                ctctATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCAAAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGc                                                                                    >  2:260666/1‑150 (MQ=255)
                                                                 tctATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCa                                                                                   >  1:722650/1‑150 (MQ=255)
                                                                  ctATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCaa                                                                                  <  1:702672/150‑1 (MQ=255)
                                                                  ctATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCaa                                                                                  >  2:833918/1‑150 (MQ=255)
                                                                   tatttcttGGCCTGCGTTACATGCGTGCGCTTGCAGCGGATCGCTTCGGTCGTCTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCaaa                                                                                 <  1:837059/150‑1 (MQ=255)
                                                                       tattGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAaacaa                                                                             >  1:977471/1‑150 (MQ=255)
                                                                         ttGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAacaaca                                                                           >  1:1147197/1‑150 (MQ=255)
                                                                         ttGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCGGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAacaaca                                                                           >  1:1499713/1‑150 (MQ=255)
                                                                           ggCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACACTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATc                                                                         >  2:1073345/1‑150 (MQ=255)
                                                                            gCCTGCGTTACATGCGTGGGCTTGCAGCGGATCGCTTCGGACGTTTCGTCTCCTGGCTTTCTACCATCGGCAATACCCTCGGCTTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACATCATcc                                                                        >  2:1711143/1‑150 (MQ=255)
                                                                            gCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATcc                                                                        >  1:1467253/1‑150 (MQ=255)
                                                                             ccTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCt                                                                       <  1:501237/150‑1 (MQ=255)
                                                                              cTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCtt                                                                      >  1:178701/1‑150 (MQ=255)
                                                                               tGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTg                                                                     >  1:1477202/1‑150 (MQ=255)
                                                                                 cGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGc                                                                   <  1:506690/150‑1 (MQ=255)
                                                                                  gTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGcc                                                                  <  2:22506/150‑1 (MQ=255)
                                                                                   ttACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCt                                                                 >  1:1195127/1‑150 (MQ=255)
                                                                                    tACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTg                                                                <  2:1367045/150‑1 (MQ=255)
                                                                                     aCATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGa                                                               >  2:400024/1‑150 (MQ=255)
                                                                                       aTGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATg                                                             <  2:691148/150‑1 (MQ=255)
                                                                                        tGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACACCATCCTAGGCCTGATGc                                                            >  2:111605/1‑150 (MQ=255)
                                                                                        tGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGc                                                            >  2:81636/1‑150 (MQ=255)
                                                                                        tGCGTGGGCGTGCAGCGGATCGCTTCGGGCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTAGTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGc                                                            <  1:279850/150‑1 (MQ=255)
                                                                                         gCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGcc                                                           <  1:1052249/150‑1 (MQ=255)
                                                                                             gggCGTGCAGCGGAGCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAg                                                       <  1:84292/150‑1 (MQ=255)
                                                                                              ggCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAgg                                                      <  2:1305118/150‑1 (MQ=255)
                                                                                              ggCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAgg                                                      <  1:12123/150‑1 (MQ=255)
                                                                                                  tGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGACGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAAt                                                  >  2:604679/1‑150 (MQ=255)
                                                                                                   gCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAAtt                                                 >  1:1699790/1‑150 (MQ=255)
                                                                                                    cAGCGGATCGCTTCGGTCGATTAGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGAATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCATTGGCCAGATGCCACAGGCAATtc                                                >  2:350508/1‑150 (MQ=255)
                                                                                                          aTCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCtctt                                          <  2:1477202/150‑1 (MQ=255)
                                                                                                              cTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTgc                                      >  1:845731/1‑149 (MQ=255)
                                                                                                              cTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGa                                      <  2:349905/150‑1 (MQ=255)
                                                                                                              cTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGa                                      >  1:1130102/1‑150 (MQ=255)
                                                                                                                tCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGACGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGc                                    <  1:604679/150‑1 (MQ=255)
                                                                                                                  ggTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCAt                                  >  2:317439/1‑150 (MQ=255)
                                                                                                                      gTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGc                                                     >  1:417198/1‑127 (MQ=255)
                                                                                                                      gTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGc                                                     <  2:417198/127‑1 (MQ=255)
                                                                                                                          cGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGctctct                          >  1:423286/1‑150 (MQ=255)
                                                                                                                                cTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAAccc                    <  1:342686/150‑1 (MQ=255)
                                                                                                                                  ggCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgc                  >  1:1064499/1‑150 (MQ=255)
                                                                                                                                   gCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgca                 >  1:930288/1‑150 (MQ=255)
                                                                                                                                    cTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcag                <  1:1110123/150‑1 (MQ=255)
                                                                                                                                    cTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcag                <  2:851015/150‑1 (MQ=255)
                                                                                                                                     tttCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTTAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc               <  2:1493307/150‑1 (MQ=255)
                                                                                                                                     tttCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc               <  2:1499713/150‑1 (MQ=255)
                                                                                                                                     tttCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc               <  2:1147197/150‑1 (MQ=255)
                                                                                                                                     tttCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc               <  2:324988/150‑1 (MQ=255)
                                                                                                                                     ctgtgaCCAGCGCCATCACCCCGAGGGTAATGCCGATGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCgcagc               >  2:1709871/6‑150 (MQ=37)
                                                                                                                                        cTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAAc            <  2:1015167/150‑1 (MQ=255)
                                                                                                                                         tACCAGCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCGGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACt           <  1:111605/150‑1 (MQ=255)
                                                                                                                                           ccATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTcc         <  2:560297/150‑1 (MQ=255)
                                                                                                                                           ccATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTcc         <  2:682359/150‑1 (MQ=255)
                                                                                                                                            cATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTccc        <  2:455741/150‑1 (MQ=255)
                                                                                                                                             aTCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCa       <  2:423286/150‑1 (MQ=255)
                                                                                                                                                  cATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGCGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAAc  >  2:356249/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CTTTCGCTTATATACTCGTGTCTTTGCTACAGCAACCAGACGGATTTCATGTACCAACCTGTCGCTCTATTTATTGGCCTGCGTTACATGCGTGGGCGTGCAGCGGATCGCTTCGGTCGTTTCGTCTCCTGGCTTTCTACCATCGGCATTACCCTCGGGGTGATGGCGCTGGTCACAGTATTGTCAGTGATGAACGGCTTTGAGCGCGAGCTGCAAAACAACATCCTTGGCCTGATGCCACAGGCAATTCTCTCTTCTGAGCATGGCTCTCTTAACCCGCAGCAACTCCCAGAAAC  >  NC_000913/1175379‑1175674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: